Mapping Local Cerebral Functional Activity by Measurement of Local Cerebral Glucose Utilization with the [14C] Deoxyglucose Method

1981 ◽  
pp. 1-18
Author(s):  
L. Sokoloff
Keyword(s):  
Functional Activity ◽  
Glucose Utilization ◽  
Local Cerebral Glucose Utilization ◽  
Cerebral Glucose Utilization ◽  
Deoxyglucose Method

scholarly journals Functional Cerebral Activity during Regeneration from Entorhinal Lesions in the Rat

1996 ◽  
Vol 16 (2) ◽  
pp. 342-352 ◽  
Author(s):  
Thomas Beck ◽  
Michael Weber ◽  
Ervin Horváth ◽  
Andreas Wree
Keyword(s):  
Functional Activity ◽  
Glucose Utilization ◽  
Recovery Period ◽  
Brain Regions ◽  
Primary Target ◽  
Local Cerebral Glucose Utilization ◽  
Brain Areas ◽  
Ipsilateral Hemisphere ◽  
Entorhinal Lesion ◽  
Deoxyglucose Method

The consequences of an unilateral electrolytic entorhinal lesion on the functional activity in all major anatomically defined brain regions were evaluated in the rat. The 14C-2-deoxyglucose method served as a tool to quantify alterations of local cerebral glucose utilization (LCGU) ipsilateral and contralateral to the lesion at 4 days, 2 weeks, or 3 months after stereotaxic surgery. Apart from a few minor increases in the contralateral hemisphere, the predominant pattern consisted of reductions in the range of 10–40% in the ipsilateral hemisphere. Ipsilaterally, in extrahippocampal areas, LCGU had regained control levels at 2 weeks postlesion in contrast to hippocampal regions, where reductions were more pronounced than in other brain areas and partially persisted for up to 3 months. Interestingly, the termination zones of entorhinal fibers in the dentate gyrus did not regain control levels within 3 months. We conclude from the data that functional recovery of denervated primary target areas does not occur within 3 months after entorhinal lesions and that altered functional activity may be found beyond the primary target areas predominantly during the acute recovery period after the lesion. The data suggest that sprouting fibers do not reestablish a fully functional neuronal network during the recovery period.

Local cerebral glucose utilization in fetal and neonatal sheep

1984 ◽  
Vol 246 (4) ◽  
pp. R608-R618 ◽  
Author(s):  
R. M. Abrams ◽  
M. Ito ◽  
J. E. Frisinger ◽  
C. S. Patlak ◽  
K. D. Pettigrew ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Glucose Utilization ◽  
Average Rate ◽  
Sensory Stimulation ◽  
Mammalian Brain ◽  
Local Cerebral Glucose Utilization ◽  
Cerebral Energy Metabolism ◽  
Cerebral Glucose Utilization ◽  
The Brain ◽  
Deoxyglucose Method

The newborn mammalian brain of several species has been shown to have a lower average rate of energy metabolism and a narrower range of rates in its various components than is found in maturity. In a further study of cerebral energy metabolism during development, we have employed the [14C]deoxyglucose method for measuring local cerebral glucose utilization in fetal and neonatal sheep. After establishing the lumped constant to be 0.40 and finding the rate constants for the kinetic behavior of deoxyglucose in plasma and brain to be close to those in other species, we measured the rates of glucose utilization in 44 regions of the brain. The rates were low and homogeneous in midgestation, except for those of brain stem nuclei of the auditory and vestibular systems and those of the hippocampus which were relatively high. In the last 7 wk, local rates rose approximately threefold. After birth there was a further average increase of 50% above full-term levels. The study shows that cerebral energy metabolism rises in most structures during prenatal maturation, a time when sensory stimulation is at a relatively low level and behavioral responses are minimal.

scholarly journals Effects of Insulin on Local Cerebral Glucose Utilization in the Rat

1987 ◽  
Vol 7 (3) ◽  
pp. 309-314 ◽  
Author(s):  
Giovanni Lucignani ◽  
Hiroki Namba ◽  
Astrid Nehlig ◽  
Linda J. Porrino ◽  
Charles Kennedy ◽  
...  
Keyword(s):  
Steady State ◽  
Glucose Utilization ◽  
Average Rate ◽  
Experimental Period ◽  
Ventromedial Nucleus ◽  
Local Cerebral Glucose Utilization ◽  
Cerebral Glucose Utilization ◽  
Hypothalamic Nuclei ◽  
The Brain ◽  
Deoxyglucose Method

The effects of hyperinsulinemia on local cerebral glucose utilization were studied by the quantitative autoradiographic 2-[14C]deoxyglucose method in normal conscious rats under steady-state normoglycemic conditions. Hyperinsulinemia and a steady state of normoglycemia were achieved and maintained during the experimental period by a continuous intravenous (i.v.) infusion of insulin given simultaneously with a programmed i.v. infusion of D-glucose. Hyperinsulinemia under normoglycemic conditions did not change the average rate of glucose utilization in the brain as a whole, but significant increases in local glucose utilization were found selectively in the ventromedial, dorsomedial, and anterior hypothalamic nuclei. The results suggest that a known anatomical pathway linking the dorsomedial and anterior nuclei with the ventromedial nucleus of the hypothalamus may be physiologically activated in response to hyperinsulinemia.

Local cerebral glucose utilization in brains of lean and genetically obese (fa/fa) rats

1993 ◽  
Vol 264 (1) ◽  
pp. E29-E36
Author(s):  
P. Doyle ◽  
F. Rohner-Jeanrenaud ◽  
B. Jeanrenaud
Keyword(s):  
Gray Matter ◽  
Functional Activity ◽  
Wistar Rats ◽  
Glucose Utilization ◽  
Local Cerebral Glucose Utilization ◽  
Brain Areas ◽  
Afferent Pathways ◽  
Cerebral Glucose Utilization ◽  
Normal Glucose ◽  
Obesity Syndrome

The local cerebral glucose utilization (LCGU) of brains from Wistar, lean Zucker (FA/FA), and obese Zucker (fa/fa) rats was investigated using the method of Sokoloff et al. (L. Sokoloff, M. Reiwich, C. Kennedy, M.H. Des Rosiers, C.S. Patlak, K.D. Pettigrew, O. Sakurada, and M. Shinohara. J. Neurochem. 28: 897–916, 1977.). The LCGU of obese Zucker (fa/fa) rats was decreased in comparison to the relatively high values obtained for the lean Zucker (FA/FA) rats in all gray matter areas studied, on average to the extent of 50%. When compared with Wistar rats, several brain areas of lean Zucker (FA/FA) animals had a normal glucose uptake. When these normal areas were assessed for common efferent and afferent pathways, it was found that many of these common connections had normal glucose utilizations. In direct comparison to the obese fa/fa rat, the LCGU rates of these areas were decreased, hinting that this would also be the case for their functional activity. Because these areas (limbic, thalamic, hypothalamic, autonomic) have been reported to be potentially relevant for bringing about the behavioral and neuroendocrine alterations known to occur in obese fa/fa rats, it is proposed that they represent dysfunctions that are partly responsible for the obesity syndrome of the fa/fa strain.

scholarly journals Increased Local Cerebral Glucose Utilization in the Basal Ganglia of the Rolling Mouse Nagoya

1982 ◽  
Vol 2 (4) ◽  
pp. 385-393 ◽  
Author(s):  
Motohiro Kato ◽  
Shinichi Hosokawa ◽  
Shozo Tobimatsu ◽  
Yoshigoro Kuroiwa
Keyword(s):  
Animal Model ◽  
Basal Ganglia ◽  
Glucose Utilization ◽  
Local Cerebral Glucose Utilization ◽  
Motor Disturbance ◽  
Cerebral Glucose Utilization ◽  
Hind Limbs ◽  
Hereditary Cerebellar Ataxia ◽  
Deoxyglucose Method ◽  
Motor Disturbances

Rolling mouse Nagoya (rolling), an experimental mutant mouse, is characterized by a marked incoordination of the hind limbs and disturbance of gait. These motor disturbances have been attributed to cerebellar dysfunction, and rolling, therefore, has been regarded as an animal model of hereditary cerebellar ataxia. However, definite evidence for this possibility has not yet been provided. In the present study, local cerebral glucose utilization (LCGU) was observed by means of the [14C]deoxyglucose method in rolling ( rol/rol), as well as in behaviorally normal heterozygotes (+/ rol) and normal controls (+/+), in order to study functional activity of the brain in these mice. A definite increase in LCGU was found in the globus pallidus, entopeduncular nucleus, subthalamic nucleus, and substantia nigra of rolling, bilaterally. A minimal decrease in LCGU was also found in the vermis of the cerebellum. These findings reflect the markedly hyperactive state of the basal ganglia and the minimally hypoactive state in the vermis of the cerebellum in rolling. It is concluded that the dysfunction in the basal ganglia is the major cause of the motor disturbances of rolling, and that rolling may be regarded as an animal model of extrapyramidal motor disturbance.

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