Proteins, peptides, and protein hydrolysates as immunomodulatory and antioxidant agents for the formulation of functional foods

2022 ◽  
pp. 137-164
Author(s):  
Andrea Araiza-Calahorra ◽  
Martin Mondor ◽  
Christine Boesch ◽  
Caroline Orfila ◽  
Francisco M. Goycoolea ◽  
...  
LWT ◽  
2013 ◽  
Vol 50 (2) ◽  
pp. 723-731 ◽  
Author(s):  
Maira R. Segura-Campos ◽  
Ine M. Salazar-Vega ◽  
Luis A. Chel-Guerrero ◽  
David A. Betancur-Ancona

Author(s):  
Sergio Moreno-Limón ◽  
Rodrigo González-Luna

Background: Nowadays, there has been an increase in the number of studies focused on the search for bioactive compounds produced by hydrolytic reactions from natural sources, such as the Quinoa grain, which represents an interesting agro-alimentary source that can have a beneficial influence on health, specifically antihypertensive potential. For this reason, the aim of the present study was to evaluate the antihypertensive activity of the protein hydrolysates obtained of Quinoa, which results important at the time to consider the incorporation of such peptides in the design of functional foods. Materials and Methods: Quinoa (Chenopodium quinoa Willd.) seeds were ground and the obtained flour was degreased and the protein isolate was obtained by isoelectric precipitation. The protein isolate was enzymatically hydrolyzed with Alcalase® and Flavourzyme® and the antihypertensive effect of peptides against angiotensin converting enzyme was evaluate using a mixture of 50 μL of sample, 50 μL of ACE working solution, 200 μL of substrate working solution and the fluorescence was determined with a microplate fluorometer following these characteristics: λ (excitation) = 355-375 nm; λ (emission) = 400-430 nm. Results: Peptides obtained using Alcalase® (protein content= 72.13%; DH= 31.22%) showed the highest inhibitory activity against the angiotensin converting enzyme (ACE), close to 88%. Conclusion: The Quinoa protein hydrolysates can be considered as a new agri-food source to be incorporated in the elaboration of functional foods with antihypertensive potential.


Author(s):  
Fathiraja Parthiban ◽  
Robinson Jeya Shakila ◽  
Geevaretnam Jeyasekaran ◽  
Rajendran Shalini

Biopeptides derived from fish protein hydrolysates are gaining popularity as active ingredients in functional foods. In this study, squid protein hydrolysates (SPH) prepared from Uroteuthis (Photololigo) duvaucelii, using alcalase and their biopeptides fractionated using ultrafiltration were investigated for their antioxidant properties in relation to the changes intheir amino acid profiles. Squid mantle hydrolysed within 30 min with a degree of hydrolysis (DH) of 13.7%. SPH had 89% 2, 2-diphenyl-1- picrylhydrazyl (DPPH) inhibition, 94% 2, 2-azino-bis-(3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) inhibition and 96% hydroxyl inhibition at 10 mg ml-1 concentration. In the squid peptides, DPPH inhibition increased with the decrease in the molecular size of peptides from 51% to 95% (3 Ka), ABTS radical inhibition was <42% for peptideswith >5KDa size and >95% for peptides with <5KDa. Hydroxyl radical inhibition increased from 75-98% with decrease in molecular size. Increase in glycine, alanine, leucine and taurine was noticed in peptides with MW <5KDa along with a decrease in methionine and arginine. Histidine, asparagine and tyrosine had also contributed to the activities of peptides with MW 3-5 KDa sizes. This study indicated that peptides with MW <5KDa had more free radicals scavenging activities with more hydrophobic amino acids, tyrosine and taurine which are mainly responsible for antioxidative properties. Therefore, squid peptides with MW <5KDa prepared using alcalase have potential to serve as nutraceuticals.


1949 ◽  
Vol 12 (4) ◽  
pp. 586-596 ◽  
Author(s):  
Mitchell Zweig ◽  
Karl A. Meyer ◽  
Frederick Steigmann
Keyword(s):  

2012 ◽  
Vol 82 (3) ◽  
pp. 144-147 ◽  
Author(s):  
Ibrahim Elmadfa ◽  
Alexa L. Meyer

A high-quality diet is one of the foundations of health and well-being. For a long time in human history, diet was chiefly a source of energy and macronutrients meant to still hunger and give the strength for work and activities that were in general much harder than nowadays. Only few persons could afford to emphasize enjoyment. In the assessment of quality, organoleptic properties were major criteria to detect spoilage and oxidative deterioration of food. Today, food hygiene is a quality aspect that is often taken for granted by consumers, despite its lack being at the origin of most food-borne diseases. The discovery of micronutrients entailed fundamental changes of the concept of diet quality. However, non-essential food components with additional health functions were still barely known or not considered important until recently. With the high burden of obesity and its associated diseases on the rise, affluent, industrialized countries have developed an increased interest in these substances, which has led to the development of functional foods to optimize special body functions, reduce disease risk, or even contribute to therapeutic approaches. Indeed, nowadays, high contents of energy, fat, and sugar are factors associated with a lower quality of food, and products with reduced amounts of these components are valued by many consumers. At the same time, enjoyment and convenience are important quality factors, presenting food manufacturers with the dilemma of reconciling low fat content and applicability with good taste and appealing appearance. Functional foods offer an approach to address this challenge. Deeper insights into nutrient-gene interactions may enable personalized nutrition adapted to the special needs of individuals. However, so far, a varied healthy diet remains the best basis for health and well-being.


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