APPLICATION OF GC/MS TO THE DETERMINATION OF NORTESTOSTERONE AND METHYLTESTOSTERONE RESIDUES IN EDIBLE TISSUES

1995 ◽  
pp. 208 ◽  
Author(s):  
C. Van Peteghem ◽  
E. Daeseleire ◽  
L. Van Look ◽  
A. De Guesquière
Keyword(s):  
Edible Tissues

Antibiotic Residues Distribute Uniformly in Broiler Chicken Breast Muscle Tissue

2008 ◽  
Vol 71 (1) ◽  
pp. 223-225 ◽  
Author(s):  
IXCHEL REYES-HERRERA ◽  
DAN J. DONOGHUE
Keyword(s):  
Muscle Tissue ◽  
Thigh Muscle ◽  
Chicken Breast ◽  
Antibiotic Residues ◽  
Monitoring Process ◽  
Significant Difference ◽  
Edible Tissues ◽  
Muscle Types ◽  
Fluoroquinolone Antibiotic

Use of antibiotics by the poultry industry has the potential to produce residues in edible tissues. In order to protect consumers, the U.S. federal government performs extensive evaluations to quantify residues in edible tissues to ensure that concentrations do not exceed the tolerance level. However, in the case of muscle tissue, the regulatory process does not differentiate between different edible muscle types in poultry. Previous studies performed by our laboratory determined higher fluoroquinolone residue concentrations in breast versus thigh muscle. Thus, if thigh tissues were used for residue monitoring, it would not accurately depict the higher concentrations. It is also possible that residue concentrations vary within tissues. To evaluate this possibility, fluoroquinolone antibiotic residues were determined for different breast sections. One hundred sixty chickens were randomly divided into four groups and dosed at 33 days of age with the fluoroquinolone antibiotic, enrofloxacin (Baytril), at either 25 ppm for 3 days, 25 ppm for 7 days, 50 ppm for 3 days, or 50 ppm for 7 days. Breast fillets were collected from each bird (n = 5 birds per day per group) during the dosing and withdrawal period. Each breast was divided into four sections (upper left, upper right, lower left, and lower right) that were analyzed as individual samples for determination of fluoroquinolone concentration. Our results indicated no significant difference (P > 0.05) in the levels of enrofloxacin residues between breast sections during the dosing or withdrawal periods. Consequently, samples can be collected from any breast section to evaluate fluoroquinolone residue concentrations during the regulatory monitoring process.

scholarly journals Determination of Residues of Flumequine and 7-Hydroxyflumequine in Edible Sheep Tissues by Liquid Chromatography with Fluorimetric and Ultraviolet Detection

1998 ◽  
Vol 81 (3) ◽  
pp. 519-527 ◽  
Author(s):  
Jean-michel Delmas ◽  
Anne-marie Chape ◽  
Pascal Sanders
Keyword(s):  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Simultaneous Determination ◽  
Rapid Method ◽  
Active Metabolite ◽  
Fluorimetric Detection ◽  
Ultraviolet Detection ◽  
Tissue Extracts ◽  
Edible Tissues

abstract A simple, sensitive, and rapid method for simultaneous determination of residues of flumequine and its microbiologically active metabolite 7-hydroxyflumequine in 100 mg sheep edible tissues (muscle, liver, kidney, and fat) by liquid chromatography is reported. After liquid-liquid cleanup with ethyl acetate, tissue extracts were injected onto a Select B column. The 2 compounds were determined by ultraviolet and fluorimetric detection. The method was repeatable and reproducible for flumequine and 7-hydroxyflumequine in muscle, liver, kidney, and fat, with limits of detection below 2 and 3 μg/kg for flumequine and 7-hydroxyflumequine, respectively. Mean recoveries for flumequine were 90 ± 7, 82 ± 7,89 ± 5, and 82 ± 6% in muscle, liver, kidney, and fat, respectively. Mean recoveries for 7-hydroxyflumequine were 91 ± 2, 90 ± 4, 86 ± 3, and 84 ± 4% in muscle, liver, kidney, and fat, respectively.

Determination of Sulfadimethoxine, Sulfamethoxazole, Trimethoprim and Their Main Metabolites in Lung and Edible Tissues from Pigs by Multi-Dimensional Liquid Chromatography

1993 ◽  
Vol 16 (1) ◽  
pp. 257-278 ◽  
Author(s):  
M. J. B. Mengelers ◽  
A. M. M. Polman ◽  
M. M. L. Aerts ◽  
H. A. Kuiper ◽  
A. S. J. P. A. M. Van Miert
Keyword(s):  
Liquid Chromatography ◽  
Edible Tissues

Simultaneous determination of cyromazine and dicyclanil in animal edible tissues using UPLC-MS/MS

2013 ◽  
Vol 30 (4) ◽  
pp. 660-665 ◽  
Author(s):  
Xiaolin Hou ◽  
Degang Zhou ◽  
Wenhui Huai ◽  
Ross C. Beier ◽  
Yingjian Sun ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Edible Tissues

Determination of Florfenicol Amine Residues in Animal Edible Tissues by an Indirect Competitive ELISA

2008 ◽  
Vol 56 (18) ◽  
pp. 8261-8267 ◽  
Author(s):  
Jin-E Wu ◽  
Chao Chang ◽  
Wen-Ping Ding ◽  
Dong-Ping He
Keyword(s):  
Competitive Elisa ◽  
Indirect Competitive Elisa ◽  
Edible Tissues

High Pressure Liquid Chromatographic Determination of Sulfamethazine in Pork Tissue

1982 ◽  
Vol 65 (6) ◽  
pp. 1311-1315
Author(s):  
Byron L Cox ◽  
Leo F Krzeminski
Keyword(s):  
High Pressure ◽  
Methylene Chloride ◽  
Trisodium Citrate ◽  
Chromatographic Determination ◽  
Hplc Method ◽  
High Pressure Liquid Chromatographic ◽  
Edible Tissues ◽  
Aqueous Mixture ◽  
Liquid Chromatographic

Abstract A high pressure liquid chromatographic (HPLC) method has been developed for determination of sulfamethazine residues in pork liver, kidney, muscle, and fat. The sample was extracted with acetone- chloroform, concentrated in the presence of dilute HC1, and partitioned between dilute HC1 and hexane. The acid solution was washed with methylene chloride and then buffered with trisodium citrate and sodium hydroxide to pH 5.8-5.9. Sulfamethazine was extracted from the aqueous mixture with methylene chloride, concentrated, dissolved in buffer, and eluted from XAD-2 resin with methanol. Sulfamethazine was reliably quantitated at 0.1 ppm by HPLC on a Zorbax ODS column with detection at 254 nm with no interference from tissues or reagents. The average recovery from the edible tissues, i.e., liver, muscle, kidney, and fat, fortified at 0.1-0.4 ppm was 85.6 ± 3.7%.

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