FLUORESCENCE BEHAVIOUR OF MOLECULAR SIZE FRACTIONS OF SUWANNEE RIVER WATER. THE EFFECT OF PHOTO-OXIDATION

1999 ◽  
pp. 157-167 ◽  
Author(s):  
T.M. Miano ◽  
J.J. Alberts
Keyword(s):  
River Water ◽  
Molecular Size ◽  
Size Fractions ◽  
Photo Oxidation ◽  
Suwannee River

scholarly journals Analysis of fluorophores of organic substances dissolved in Suvani river water using reversed-phase liquid chromatography

2019 ◽  
Vol 46 (4) ◽  
pp. 428-437
Author(s):  
O. A. Trubetskoy ◽  
O. E. Trubetskaya
Keyword(s):  
Liquid Chromatography ◽  
River Water ◽  
Natural Water ◽  
High Efficiency ◽  
Aromatic Amino Acids ◽  
Molecular Size ◽  
Reversed Phase ◽  
Size Exclusion ◽  
Organic Substances ◽  
Suwannee River

Reversed-phase high-efficiency liquid chromatography was used in combination with detection by multiwave fluorescence for analysis of organic substances dissolved in natural water of the Suwannee River. Also analyzed were the stable electrophoretic fractions А, В, and C+D, obtained by a combination of preparative size-exclusion chromatography and analytical electrophoresis in a polyacrylamide gel. Fraction А has the largest molecular size, and fraction C+D, the smallest. Using 3D fluorescent analysis, humic-like fluorescence was detected both in the original sample and in all fractions; protein-like fluorescence is almost fully localized in fractions А and В of the largest and middle molecular sizes. The wide peak of humic-like fluorescence is split into several groups of fluorophores with different emissions maxima (435, 455, 460, and 465 nm) and degrees of hydrophobicity. The obtained results were analyzed in relation to contemporary theories of formation of humic-like fluorescence of dissolved organic substances. The low-molecular free aromatic amino acids tyrosine and tryptophan were identified in fractions А and В of the highest molecular size and constitute >50% of the protein-like fluorescence of the organic substances dissolved in the Suwannee River water. The data obtained ensure better understanding of the molecular nature of protein-like and humic-like fluorescence of organic substance dissolved in natural water.

RP-HPLC and spectroscopic characterization of Suwannee River water NOM after concentrated urea treatment and dialysis

2015 ◽  
Vol 57 (12) ◽  
pp. 5358-5364 ◽  
Author(s):  
Olga E. Trubetskaya ◽  
Claire Richard ◽  
Guillaume Voyard ◽  
Victor V. Marchenkov ◽  
Oleg A. Trubetskoj
Keyword(s):  
River Water ◽  
Spectroscopic Characterization ◽  
Urea Treatment ◽  
Rp Hplc ◽  
Suwannee River

scholarly journals Structural studies on sialylated and sulphated O-glycosidic mannose-linked oligosaccharides in the chondroitin sulphate proteoglycan of brain

1987 ◽  
Vol 245 (1) ◽  
pp. 229-234 ◽  
Author(s):  
T Krusius ◽  
V N Reinhold ◽  
R K Margolis ◽  
R U Margolis
Keyword(s):  
Ion Exchange ◽  
Chondroitin Sulphate ◽  
Gel Filtration ◽  
Molecular Size ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Keratan Sulphate ◽  
Size Fractions ◽  
Sulphate Proteoglycan ◽  
Chondroitin Sulphate Proteoglycan

We have previously described the structures of neutral and sialylated O-glycosidic mannose-linked tetrasaccharides and keratan sulphate polysaccharide chains in the chondroitin sulphate proteoglycan of brain. The present paper provides information on a series of related sialylated and/or sulphated tri- to penta-saccharides released by alkaline-borohydride treatment of the proteoglycan glycopeptides. The oligosaccharides were fractionated by ion-exchange chromatography and gel filtration, and their structural properties were studied by methylation analysis and fast-atom-bombardment mass spectrometry. Five fractions containing [35S]sulphate-labelled oligosaccharides were obtained by ion-exchange chromatography, each of which was eluted from Sephadex G-50 as two well-separated peaks. The apparent Mr values of both the large- and small-molecular-size fractions increased with increasing acidity (and sulphate labelling) of the oligosaccharides. The larger-molecular-size fractions contained short mannose-linked keratan sulphate chains of Mr 3000-4500, together with some asparagine-linked oligosaccharides. The smaller tri- to penta-saccharides, of Mr 800-1400, appear to have a common GlcNac(beta 1-3)Manol core, and to contain one to two residues of sialic acid and/or sulphate.

Isotherm and kinetic studies on the adsorption of humic acid molecular size fractions onto clay minerals

2019 ◽  
Vol 38 (6) ◽  
pp. 863-871 ◽  
Author(s):  
Mohamed E. A. El-Sayed ◽  
Moustafa M. R. Khalaf ◽  
James A. Rice
Keyword(s):  
Humic Acid ◽  
Clay Minerals ◽  
Molecular Size ◽  
Kinetic Studies ◽  
Size Fractions
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