Amperometric glucose oxidase/hydrogen peroxide glucose sensors: the influence of hydrogen peroxide on the function and its potential use in sensor sterilization

1992 ◽  
pp. 513
Author(s):  
Th.v. Woedtke ◽  
P. Abel ◽  
U. Fischer
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Glucose Sensors ◽  
Potential Use

Preparation of micro-biosensor for continuous glucose monitoring

2015 ◽  
Vol 29 (06n07) ◽  
pp. 1540040
Author(s):  
Mikito Yasuzawa ◽  
Taketo Toba ◽  
Kentaro Hiura ◽  
Jiang Li ◽  
Pankaj M. Koinkar ◽  
...  
Keyword(s):  
Thin Film ◽  
Hydrogen Peroxide ◽  
Carbon Fiber ◽  
Glucose Oxidase ◽  
Biological Fluid ◽  
Glucose Monitoring ◽  
Glucose Sensors ◽  
Oxidation Current ◽  
Carbon Fiber Electrodes ◽  
Platinum Thin Film

Amperometric glucose sensors with a diameter of less than 10 μm were fabricated by the immobilization of glucose oxidase ( GOx ) on carbon fiber electrodes. Carbon fiber electrodes with platinum thin film were also prepared using electroplating and sputtering. The combination of electrodeposition and electropolymerization was employed for the immobilization of GOx . Although the introduction of Pt film satisfactorily improved the oxidation current of the electrode to hydrogen peroxide, sensitivities of obtained GOx -immobilized electrodes to glucose were not significant. Formation of electroplated-platinum thin film on carbon fiber was effective to reduce the influence of electroactive compounds existing in biological fluid such as ascorbic and uric acids.

scholarly journals Enzymatically triggered rupture of polymersomes

Soft Matter ◽  
2016 ◽  
Vol 12 (4) ◽  
pp. 1014-1020 ◽  
Author(s):  
Woo-Sik Jang ◽  
Seung Chul Park ◽  
Ellen H. Reed ◽  
Kevin P. Dooley ◽  
Samuel F. Wheeler ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase

Polymersomes are robust vesicles made from di-block co-polymers. We have engineered a two step enzymatic cascade to trigger the release of contents from polymersomes, in which extravesicular glucose oxidase makes hydrogen peroxide, when then penetrates the membrane and is converted by entrapped catalase to oxygen, leading to vesicle failure.

Dual oxygen and Ir oxide regeneration of glucose oxidase in nanostructured thin film glucose sensors

2010 ◽  
Vol 55 (26) ◽  
pp. 7683-7689 ◽  
Author(s):  
Amit S. Jhas ◽  
Hanna Elzanowska ◽  
Bri Sebastian ◽  
Viola Birss
Keyword(s):  
Thin Film ◽  
Glucose Oxidase ◽  
Glucose Sensors ◽  
Nanostructured Thin Film

scholarly journals Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

Polymers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 319 ◽  
Author(s):  
Kentaro Yoshida ◽  
Yu Kashimura ◽  
Toshio Kamijo ◽  
Tetsuya Ono ◽  
Takenori Dairaku ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase ◽  
Dna Cleavage ◽  
Electrostatic Interactions ◽  
Glucose Solution ◽  
Layer By Layer ◽  
Sensitive Layer ◽  
Physiological Conditions ◽  
Lbl Films ◽  
Low Glucose

Glucose-sensitive films were prepared through the layer-by-layer (LbL) deposition of hemin-modified poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx)). H-PEI/DNA + GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA + GOx)5 film was then partially decomposed by hydrogen peroxide (H2O2). The mechanism for the decomposition of the LbL film was considered to involve more reactive oxygen species (ROS) that were formed by the reaction of hemin and H2O2, which then caused nonspecific DNA cleavage. In addition, GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the (H-PEI/DNA + GOx)5 film was observed when the thin film was immersed in a glucose solution. (H-PEI/DNA + GOx)5 films exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated that the decomposition of the film increased with the time to 9.97%, 16.3%, 23.1%, and 30.5%, respectively. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strengths close to physiological conditions, it was possible to slowly decompose the LbL film at low glucose concentrations of 1–10 mM.

Nitroalkanes as Reductive Substrates for Flavoprotein Oxidases

1972 ◽  
Vol 27 (9) ◽  
pp. 1052-1053 ◽  
Author(s):  
David J. T. Porter ◽  
Judith G. Voet ◽  
Harold J. Bright
Keyword(s):  
Hydrogen Peroxide ◽  
Amino Acid ◽  
Glucose Oxidase ◽  
Ph Dependence ◽  
Kinetic Mechanism ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Kinetics Of ◽  
Oxidase Reaction ◽  
Detailed Kinetic Mechanism

Nitroalkanes have been found to be general reductive substrates for D-amino acid oxidase, glucose oxidase and L-amino acid oxidase. These enzymes show different specificities for the structure of the nitroalkane substrate.The stoichiometry of the D-amino acid oxidase reaction is straightforward, consisting of the production of one mole each of aldehyde, nitrite and hydrogen peroxide for each mole of nitroalkane and oxygen consumed. The stoichiometry of the glucose oxidase reaction is more complex in that less than one mole of hydrogen peroxide and nitrite is produced and nitrate and traces of 1-dinitroalkane are formed.The kinetics of nitroalkane oxidation show that the nitroalkane anion is much more reactive in reducing the flavin than is the neutral substrate. The pH dependence of flavin reduction strongly suggests that proton abstraction is a necessary event in catalysis. A detailed kinetic mechanism is presented for the oxidation of nitroethane by glucose.It has been possible to trap a form of modified flavin in the reaction of D-amino acid oxidase with nitromethane from which oxidized FAD can be regenerated in aqueous solution in the presence of oxygen.

Permselective monolayer membrane based on two-dimensional cross-linked polysiloxane LB films for hydrogen peroxide detecting glucose sensors

2002 ◽  
pp. 2616-2617 ◽  
Author(s):  
Dai Kato ◽  
Mio Masaike ◽  
Takahito Majima ◽  
Yoshiki Hirata ◽  
Fumio Mizutani ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Sensors ◽  
Two Dimensional ◽  
Lb Films

Photocatalytic Treatment of Wastewater From 5–Fluorouracil Manufacturing

1996 ◽  
Vol 118 (1) ◽  
pp. 2-8 ◽  
Author(s):  
M. Anheden ◽  
D. Y. Goswami ◽  
G. Svedberg
Keyword(s):  
Hydrogen Peroxide ◽  
Photocatalytic Oxidation ◽  
Uv Light ◽  
Substantial Reduction ◽  
Reduction Rate ◽  
Reaction Rates ◽  
Batch Experiments ◽  
Cod Reduction ◽  
Potential Use ◽  
Percent Decrease

This paper presents some of the experimental results from a study conducted to demonstrate the potential use of photocatalytic oxidation for decolorization and COD reduction of wastewater from 5–fluorouracil manufacturing. A series of batch experiments, were carried out using diluted solutions of the wastewater with 0.1 percent w/v TiO2. Low pressure mercury lamps were used to simulate the UV part of sunlight. The experiments showed that a complete decolorization and a substantial reduction of COD was achieved within 20 hours with a 20 percent solution. During the reaction period, the pH was noted to decrease considerably, indicating formation of acids. Adding hydrogen peroxide to the solution was found to significantly increase the reaction rates. Adding 2400 ppm of H2O2 gave an 80 percent decrease in color in one hour and a 70-80 percent decrease in COD in 20 hours. The influence of UV-light intensity was also examined. This experiment showed that with a UV-intensity of 15 W/m2, i.e., a cloudy day, the decolorization rate was still considerable, while the COD reduction rate was very low.

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