Dual-mode fluorescent and colorimetric immunoassay for the ultrasensitive detection of alpha-fetoprotein in serum samples

Abstract A sandwich-type heterogeneous enzyme immunoassay with flow-injection analysis for alpha-fetoprotein (AFP) in human serum has been developed. 4-Aminophenol, the product of enzymatic reaction, is detected amperometrically. The interassay CV for this electrochemical enzyme immunoassay was less than 8.2%, with a minimum detection limit for AFP of 0.163 micrograms/L. The calibration curve had a linear range of 0.316-100 micrograms/L. Studies with 48 human maternal serum samples, comparing results by this method with those by a commercial kit, showed a good correlation (r = 0.961). This procedure provides an alternative method for determining low concentrations of AFP in human maternal serum.

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A novel electrochemical immunosensor using β-cyclodextrins functionalized silver supported adamantine-modified glucose oxidase as labels for ultrasensitive detection of alpha-fetoprotein

Analytica Chimica Acta ◽

10.1016/j.aca.2015.08.052 ◽

2015 ◽

Vol 893 ◽

pp. 49-56 ◽

Cited By ~ 20

Author(s):

Jian Gao ◽

Hongmin Ma ◽

Xiaohui Lv ◽

Tao Yan ◽

Na Li ◽

...

Keyword(s):

Glucose Oxidase ◽

Electrochemical Immunosensor ◽

Alpha Fetoprotein ◽

Ultrasensitive Detection

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Improvement and Multicenter Evaluation of the Analytical Performance of an Automated Chemiluminescent Immunoassay for Alpha Fetoprotein

The International Journal of Biological Markers ◽

10.5301/jbm.2011.8738 ◽

2012 ◽

Vol 27 (1) ◽

pp. 39-46 ◽

Cited By ~ 5

Author(s):

Kaori Morota ◽

Makoto Komori ◽

Ryo Fujinami ◽

Koji Yamada ◽

Kageaki Kuribayashi ◽

...

Keyword(s):

Medical Center ◽

Correlation Coefficients ◽

Alpha Fetoprotein ◽

Analytical Performance ◽

Serum Samples ◽

Johns Hopkins University ◽

Limit Of Quantitation ◽

Chemiluminescent Immunoassay ◽

Regression Slopes ◽

Medical University

Background A new ARCHITECT® alpha fetoprotein (AFP) assay was developed to improve the linearity at the upper end of the calibration curve and to enhance other performance characteristics. In addition, this reformulation eliminated the possibility of falsely depressed samples at high AFP concentrations. The purpose of this study was to evaluate its analytical performance at multiple sites. Methods The assay configuration, the diluent formulation, and the manufacturing process were redesigned. Analytical performance was evaluated at Abbott Laboratories, Sapporo Medical University, VU University Medical Center, and Johns Hopkins University. Results The limit of quantitation of the assay was 1.00–1.30 ng/mL. Total precision (%CV) across the assay range varied between 1.41 and 3.52. The assay was linear from 1.19 to 2535 ng/mL, and the range of the assay was expanded from 200 ng/mL to 2000 ng/mL. Comparison of this assay with the on-market ARCHITECT, AxSYM, ADVIA Centaur, DxI, AIA-1800, and E 170 systems yielded regression slopes of 0.91–1.08 and correlation coefficients of ≥0.99 for serum samples. No falsely depressed results were observed in 174 serum samples with AFP concentrations of 2018–1,196,856 ng/mL and in a spiked sample containing up to 10 mg/mL of purified AFP. Conclusions The new AFP assay has improved an issue of the on-market ARCHITECT AFP assay and demonstrated excellent assay performance.

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A Novel Amperometric Immunosensor for Detection of Alpha-Fetoprotein Based on Magnetic and Electroactive Nanoprobes

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.345.411 ◽

2011 ◽

Vol 345 ◽

pp. 411-416

Author(s):

Ling Hua Meng ◽

Yu Ting Cao ◽

Fu Tao Hu ◽

Tian Hua Li ◽

Ning Gan

Keyword(s):

Signal To Noise Ratio ◽

Three Dimensional ◽

Alpha Fetoprotein ◽

Serum Samples ◽

Electron Mediator ◽

Experimental Conditions ◽

Au Nps ◽

Transmission Electron ◽

Walled Carbon Nanotubes ◽

First Time

A novel three-dimensional, magnetic and electroactive nanoprobes were constructed for the first time. Using hemin (TPP) as electron mediator, mutli-walled carbon nanotubes with carboxyl groups (MCNTs) as supporter, a novel (MCNTs-TPP-Fe3O4)magnetic nanocomposites were first prepared. Then using alpha-fetoprotein (AFP) as model system, gold nanoparticles (Au NPs) as immobilizing matrix, the AFP/anti-AFP/Au NPs/ MCNTs-TPP-Fe3O4nanoprobes were prepared and then dropped on the surface of screen-printed working electrode (SPCE) to construct a new amperometric immunosensors for detecting biomakers. The microstructure of different nanoparticles were observed by transmission electron microscopy (TEM) andX-ray fluorescence spectrometery (XRFS). Under optimal experimental conditions, the logarithm of response signal was proportional to the logarithm of AFP concentration from 0.1 to 200 ng/mL, with a correlation coefficient of 0.998. The detection limit was 0.04 ng/mL at a signal-to-noise ratio of 3. The proposed method offered a platform for fast, sensitive and simultaneous determination for serum samples.

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Giant Gold Nanowire Vesicle-Based Colorimetric and SERS Dual-Mode Immunosensor for Ultrasensitive Detection of Vibrio parahemolyticus

Analytical Chemistry ◽

10.1021/acs.analchem.8b00292 ◽

2018 ◽

Vol 90 (10) ◽

pp. 6124-6130 ◽

Cited By ~ 25

Author(s):

Zhiyong Guo ◽

Yaru Jia ◽

Xinxin Song ◽

Jing Lu ◽

Xuefei Lu ◽

...

Keyword(s):

Ultrasensitive Detection ◽

Dual Mode ◽

Gold Nanowire ◽

Vibrio Parahemolyticus

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A dual-mode fluorescent and colorimetric immunoassay based on in situ ascorbic acid-induced signal generation from metal-organic frameworks

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2019.127180 ◽

2020 ◽

Vol 302 ◽

pp. 127180 ◽

Cited By ~ 4

Author(s):

Wenyue Xie ◽

Meiling Tian ◽

Xian Luo ◽

Ying Jiang ◽

Na He ◽

...

Keyword(s):

Ascorbic Acid ◽

Metal Organic Frameworks ◽

Signal Generation ◽

Dual Mode ◽

Metal Organic ◽

Colorimetric Immunoassay ◽

Induced Signal

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Development and application of a fluorescence protein microarray for detecting serum alpha-fetoprotein in patients with hepatocellular carcinoma

Journal of International Medical Research ◽

10.1177/0300060516672370 ◽

2016 ◽

Vol 44 (6) ◽

pp. 1414-1423 ◽

Cited By ~ 1

Author(s):

Aiying Zhang ◽

Chengzeng Yin ◽

Zhenshun Wang ◽

Yonghong Zhang ◽

Yuanshun Zhao ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Protein Microarray ◽

Characteristic Curve ◽

Alpha Fetoprotein ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Control Subjects ◽

Serum Alpha Fetoprotein ◽

Healthy Control ◽

Fluorescence Protein

Objective To develop a simple, effective, time-saving and low-cost fluorescence protein microarray method for detecting serum alpha-fetoprotein (AFP) in patients with hepatocellular carcinoma (HCC). Method Non-contact piezoelectric print techniques were applied to fluorescence protein microarray to reduce the cost of prey antibody. Serum samples from patients with HCC and healthy control subjects were collected and evaluated for the presence of AFP using a novel fluorescence protein microarray. To validate the fluorescence protein microarray, serum samples were tested for AFP using an enzyme-linked immunosorbent assay (ELISA). Results A total of 110 serum samples from patients with HCC ( n = 65) and healthy control subjects ( n = 45) were analysed. When the AFP cut-off value was set at 20 ng/ml, the fluorescence protein microarray had a sensitivity of 91.67% and a specificity of 93.24% for detecting serum AFP. Serum AFP quantified via fluorescence protein microarray had a similar diagnostic performance compared with ELISA in distinguishing patients with HCC from healthy control subjects (area under receiver operating characteristic curve: 0.906 for fluorescence protein microarray; 0.880 for ELISA). Conclusion A fluorescence protein microarray method was developed for detecting serum AFP in patients with HCC.

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