Pyrolytic profiling nitrosamine specific chemiluminescence detection combined with multivariate chemometric discrimination for non-targeted detection and classification of nitroso compounds in complex samples

Abstract The modification of a newly developed method for determination of apparent total N-nitroso compounds by chemical denitrosation and chemiluminescence detection of nitric oxide (thermal energy analysis) is described. The minimum level of reliable measurement was 0.1 ppm, and the repeatability of the method was 0.2 ppm, based on the response of N-nitrosoproline (NPro). Seventy-three samples of cured-meat products, including frankfurters, bacon, and ham, were examined; 50 samples contained less than 1 ppm. The largest amounts, up to 24.8 ppm, were detected in canned corned beef. This method has several advantages over other methods.

Download Full-text

A Rapid and Selective Method for Determining Potential Nitrosating Agents in Cosmetic Products by Chemiluminescence Detection of Nitric Oxide

Journal of AOAC International ◽

10.1093/jaoac/81.2.368 ◽

1998 ◽

Vol 81 (2) ◽

pp. 368-372 ◽

Cited By ~ 4

Author(s):

Hardy J Chou ◽

Ronald L Yates

Keyword(s):

Nitric Oxide ◽

Detection Limit ◽

False Positive ◽

Nitroso Compounds ◽

Cosmetic Products ◽

Chemiluminescence Detection ◽

Selective Method ◽

Selective Determination ◽

Chemiluminescent Reaction

abstract A method was developed for rapid and selective determination of potential nitrosating agents at the part-per-billion level in cosmetic products. These compounds are chemically reduced to nitric oxide, which is determined by its chemiluminescent reaction with ozone. Suspended materials and colors in cosmetic products do not interfere. Hence their removal before analysis is not required. A detection limit of 33 ppb, calculated as nitrite, was obtained. No false-positive interferences were observed from antifoaming agents, several AZ-nitroso compounds, and nitrate up to 20 ppm. Among cosmetic products surveyed, potential nitrosating agents were found at levels ranging from 113 to 5021 ppb. No consistent relationship was found between levels of potential nitrosating agents and N-nitrosamines in the same products. However, the highest levels of nitrosating agents were most often associated with the highest levels of N-nitrosamines known to be present in the products.

Download Full-text

Separation of assay of N-nitroso compounds by high-performance liquid chromatography with chemiluminescence detection

Journal of Chromatography A ◽

10.1016/s0021-9673(01)84471-x ◽

1989 ◽

Vol 463 ◽

pp. 201-206 ◽

Cited By ~ 6

Author(s):

C. Pinche ◽

J.P. Billard ◽

A.M. Frasey ◽

H. Bargnoux ◽

J. Petit ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Nitroso Compounds ◽

Chemiluminescence Detection

Download Full-text

Using the Doubly Charged Selected Ion Coupled with MS/MS Fragments Monitoring (DCSI-MS/MS) Mode for the Identification of Gelatin Species

Journal of Analytical Methods in Chemistry ◽

10.1155/2014/764397 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 4

Author(s):

Xian-Long Cheng ◽

Feng Wei ◽

Jia Chen ◽

Ming-hua Li ◽

Lei Zhang ◽

...

Keyword(s):

Ion Trap ◽

Time Interval ◽

Complex Samples ◽

Ion Trap Mass Spectrometer ◽

Multiply Charged ◽

Species Specific ◽

Doubly Charged ◽

Charged Ions ◽

Hydrolysis Of

In electrospray ionization (ESI) mode, peptides and proteins can be multiply charged ions; in this situation a doubly charged selected ion (DCSI) coupled with mass spectrometry (MS/MS) fragments monitoring (DCSI-MS/MS) method is the most suitable scanning mode to detect known peptides in complex samples when an ion-trap mass spectrometer is the instrument used for the analysis. In this mode, the MS detector is programmed to only select a doubly charged ion as a precursor and to perform continuous MS/MS on one or more of the selected precursors, either during a specific time interval or along the whole chromatographic run. Gelatin is a mixture of high molecular weight polypeptides from the hydrolysis of collagen. In this study, the DCSI-MS/MS monitoring mode was applied to the detection of previously characterized species-specific peptides from different gelatins. The proposed methodology makes use of tryptic digestion for sample preparation and peptide separation and identification by rapid resolution liquid chromatography coupled to an ion trap working in the DCSI-MS/MS mode for the analysis. This methodology was applied to the differential classification of five commercial, homological species of gelatins and proved to be an excellent tool for gelatin product authentication.

Download Full-text