Low genetic variation in Echinococcus multilocularis from the Western Sichuan Plateau of China revealed by microsatellite and mitochondrial DNA markers

Acta Tropica ◽  
2021 ◽  
pp. 105989
Author(s):  
Jing-Ye Shang ◽  
Guang-Jia Zhang ◽  
Sha Liao ◽  
Wen-Jie Yu ◽  
Wei He ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Dna Markers ◽  
Echinococcus Multilocularis ◽  
Western Sichuan

scholarly journals First molecular description, phylogeny and genetic variation of Taenia hydatigena from Nigerian sheep and goats based on three mitochondrial genes

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
John A. Ohiolei ◽  
Joshua Luka ◽  
Guo-Qiang Zhu ◽  
Hong-Bin Yan ◽  
Li Li ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Dna Markers ◽  
Nadh Dehydrogenase ◽  
Mitochondrial Genes ◽  
Full Length ◽  
Nadh Dehydrogenase Subunit ◽  
Taenia Hydatigena ◽  
Sheep And Goats ◽  
Geographical Regions

Abstract Background Cysticercosis caused by the metacestode larval stage of Taenia hydatigena is a disease of veterinary and economic importance. A considerable level of genetic variation among isolates of different intermediate hosts and locations has been documented. Generally, data on the genetic population structure of T. hydatigena is scanty and lacking in Nigeria. Meanwhile, similar findings in other cestodes like Echinococcus spp. have been found to be of epidemiological importance. Our aim, therefore, was to characterize and compare the genetic diversity of T. hydatigena population in Nigeria based on three mitochondrial DNA markers as well as to assess the phylogenetic relationship with populations from other geographical regions. Methods In the present study, we described the genetic variation and diversity of T. hydatigena isolates from Nigerian sheep and goats using three full-length mitochondrial genes: the cytochrome c oxidase subunit 1 (cox1), NADH dehydrogenase subunit 1 (nad1), and NADH dehydrogenase subunit 5 (nad5). Results The median-joining network of concatenated cox1-nad1-nad5 sequences indicated that T. hydatigena metacestodes of sheep origin were genetically distinct from those obtained in goats and this was supported by high FST values of nad1, cox1, and concatenated cox1-nad1-nad5 sequences. Genetic variation was also found to be higher in isolates from goats than from sheep. Conclusions To the best of our knowledge, the present study described the genetic variation of T. hydatigena population for the first time in Nigeria using full-length mitochondrial genes and suggests the existence of host-specific variants. The population indices of the different DNA markers suggest that analysis of long mitochondrial DNA fragments may provide more information on the molecular ecology of T. hydatigena. We recommend that future studies employ long mitochondrial DNA sequence in order to provide reliable data that would explain the extent of genetic variation in different hosts/locations and the biological and epidemiological significance.

Haplogroup Structure and Genetic Variation Analyses of 60 Mitochondrial DNA Markers in Southern Shaanxi Han Population

2019 ◽  
Vol 58 (2) ◽  
pp. 279-293 ◽  
Author(s):  
Yuan-Yuan Wei ◽  
Zhan-Ping Ren ◽  
Xiao-Ye Jin ◽  
Wei Cui ◽  
Chong Chen ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variation ◽  
Dna Markers ◽  
Han Population ◽  
Southern Shaanxi

scholarly journals First Insight into Groundwater Copepods of the Polish Lowland

Water ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2086
Author(s):  
Maciej Karpowicz ◽  
Sabina Smolska ◽  
Magdalena Świsłocka ◽  
Joanna Moroz
Keyword(s):  
Mitochondrial Dna ◽  
Ribosomal Rna ◽  
Dna Markers ◽  
Coi Gene ◽  
Molecular Characteristics ◽  
Morphological Description ◽  
Single Plate ◽  
North Eastern ◽  
Insight Into

Our results are the first insight into groundwater copepods of the Polish Lowland. The sampling was conducted in 28 wells in north-eastern Poland, and Copepoda were present in 16 wells. We have identified six Copepoda species and one Cladocera. We have classified four species as stygophiles—Eucyclops serrulatus, Diacyclops bisetosus, Diacyclops crassicaudis, and Cyclops furcifer. These species were frequently found in studied wells of different regions of north-eastern Poland, often in high numbers, and females with egg sacs were observed. We present a detailed morphological description of these species, together with molecular characteristics based on mitochondrial DNA markers (COI gene) for E. serrulatus, D. bisetosus, and D. crassicaudis, and 12S ribosomal RNA for C. furcifer. We also present the development of abnormal structures in one specimen of D. crassicaudis, where the upper part of furcal rami was fused to form a single plate.

scholarly journals Genetic Variation among and within United States Collard Cultivars and Landraces as Determined by Randomly Amplified Polymorphic DNA Markers

1996 ◽  
Vol 121 (3) ◽  
pp. 374-379 ◽  
Author(s):  
Mark W. Farnham
Keyword(s):  
Genetic Variation ◽  
Dna Markers ◽  
Crop Improvement ◽  
Similarity Index ◽  
Randomly Amplified Polymorphic Dna ◽  
Brussels Sprouts ◽  
Breeding Lines ◽  
Intrapopulation Variation ◽  
Similarity Indices ◽  
Systematic Collection

A collection of collard (Brassica oleracea L., Acephala group) germplasm, including 13 cultivars or breeding lines and 5 landraces, was evaluated using randomly amplified polymorphic DNA (RAPD) markers and compared to representatives of kale (Acephala group), cabbage (Capitata group), broccoli (Italica group), Brussels sprouts (Gemmifera group), and cauliflower (Botrytis group). Objectives were to assess genetic variation and relationships among collard and other crop entries, evaluate intrapopulation variation of open-pollinated (OP) collard lines, and determine the potential of collard landraces to provide new B. oleracea genes. Two hundred nine RAPD bands were scored from 18 oligonucleotide decamer primers when collard and other B. oleracea entries were compared. Of these, 147 (70%) were polymorphic and 29 were specific to collard. Similarity indices between collard entries were computed from RAPD data and these ranged from 0.75 to 0.99 with an average of 0.83. Collard entries were most closely related to cabbage (similarity index = 0.83) and Brussels sprouts entries (index = 0.80). Analysis of individuals of an OP cultivar and landrace indicated that intrapopulation genetic variance accounts for as much variation as that observed between populations. RAPD analysis identified collard landraces as unique genotypes and showed them to be sources of unique DNA markers. The systematic collection of collard landraces should enhance diversity of the B. oleracea germplasm pool and provide genes for future crop improvement.

scholarly journals Molecular Methods for the Detection of Natural Hybrids in Sturgeon Populations

2015 ◽  
Vol 16 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Alexandru Burcea ◽  
Iulia Elena Florescu ◽  
Andreea Dudu ◽  
Sergiu Emil Georgescu ◽  
Marieta Costache
Keyword(s):  
Mitochondrial Dna ◽  
Dna Markers ◽  
Molecular Methods ◽  
Hybrid Species ◽  
Habitat Area ◽  
Natural Hybrids ◽  
Sturgeon Species ◽  
Morphological Criteria ◽  
Hybrid Sturgeon

Abstract Due to construction of the Iron Gates dams, the Lower Danube has suffered a decrease in sturgeon populations. The dams have decreased sturgeon habitat area, which in turn has caused an overlap of reproduction areas for all sturgeon species. The ease with which sturgeon species can create hybrid offsprings gave rise to an increase in the number of hybrid sturgeon species now found in the Lower Danube area. We propose a set of molecular methods for hybrid species using DNA markers represented by microsatellites and mitochondrial DNA. This identification data and methodology is important for use on sturgeon farms due to the need to correctly identify species of sturgeons. Using the proposed methodologies, it is possible to avoid identification errors that might appear when using only morphological criteria to idenfy sturgeons

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