Scaffold-mediated sequential drug/gene delivery to promote nerve regeneration and remyelination following traumatic nerve injuries

Objective. Guiding Regeneration Gel (GRG) was developed in response to the clinical need of improving treatment for peripheral nerve injuries and helping patients regenerate massive regional losses in peripheral nerves. The efficacy of GRG based on tissue engineering technology for the treatment of complete peripheral nerve injury with significant loss defect was investigated.Background. Many severe peripheral nerve injuries can only be treated through surgical reconstructive procedures. Such procedures are challenging, since functional recovery is slow and can be unsatisfactory. One of the most promising solutions already in clinical practice is synthetic nerve conduits connecting the ends of damaged nerve supporting nerve regeneration. However, this solution still does not enable recovery of massive nerve loss defect.The proposed technologyis a biocompatible and biodegradable gel enhancing axonal growth and nerve regeneration. It is composed of a complex of substances comprising transparent, highly viscous gel resembling the extracellular matrix that is almost impermeable to liquids and gasses, flexible, elastic, malleable, and adaptable to various shapes and formats.Preclinical studyon rat model of peripheral nerve injury showed that GRG enhanced nerve regeneration when placed in nerve conduits, enabling recovery of massive nerve loss, previously unbridgeable, and enabled nerve regeneration at least as good as with autologous nerve graft “gold standard” treatment.

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Three-dimensional scaffold platform for controlled therapeutic drug and non-viral gene delivery to enhance nerve regeneration and remyelination in spinal cord injuries

10.32657/10356/74525 ◽

2018 ◽

Author(s):

◽

Lan Huong Nguyen

Keyword(s):

Spinal Cord ◽

Gene Delivery ◽

Nerve Regeneration ◽

Spinal Cord Injuries ◽

Three Dimensional ◽

Therapeutic Drug ◽

Viral Gene ◽

Viral Gene Delivery

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Controlled release of ciliary neurotrophic factor from bioactive nerve grafts promotes nerve regeneration in rats with facial nerve injuries

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.37327 ◽

2021 ◽

Author(s):

Fukai Ma ◽

Hanming Wang ◽

Xiaosheng Yang ◽

Yiwei Wu ◽

Chenlong Liao ◽

...

Keyword(s):

Controlled Release ◽

Facial Nerve ◽

Nerve Regeneration ◽

Neurotrophic Factor ◽

Ciliary Neurotrophic Factor ◽

Nerve Injuries ◽

Nerve Grafts ◽

Facial Nerve Injuries

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Advances in electrical and magnetic stimulation on nerve regeneration

Regenerative Medicine ◽

10.2217/rme-2018-0079 ◽

2019 ◽

Vol 14 (10) ◽

pp. 969-979 ◽

Cited By ~ 5

Author(s):

Yun Qian ◽

Yuan Cheng ◽

Jiangyu Cai ◽

Xiaotian Zhao ◽

Yuanming Ouyang ◽

...

Keyword(s):

Nerve Regeneration ◽

Magnetic Stimulation ◽

Clinical Practices ◽

Nerve Injuries ◽

Physical Stimulation ◽

Ion Channel Regulation ◽

Biomaterial Scaffolds ◽

Underlying Mechanisms ◽

Factor Secretion ◽

Growth Factor Secretion

Central and peripheral nerve injuries pose a great threat to people. Complications such as inflammation, muscle atrophy, traumatic neuromas and delayed reinnervation can bring huge challenges to clinical practices and barriers to complete nerve regrowth. Physical interventions such as electrical and magnetic stimulation show satisfactory results with varying parameters for acute and chronic nerve damages. The biological basis of electrical and magnetic stimulation mainly relies on protein synthesis, ion channel regulation and growth factor secretion. This review focuses on the various paradigms used in different models of electrical and magnetic stimulation and their regenerative potentials and underlying mechanisms in nerve injuries. The combination of physical stimulation and conductive biomaterial scaffolds displays an infinite potentiality in translational application in nerve regeneration.

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Sciatic nerve regeneration induced by glycosaminoglycan and laminin mimetic peptide nanofiber gels

RSC Advances ◽

10.1039/c6ra24450e ◽

2016 ◽

Vol 6 (112) ◽

pp. 110535-110547 ◽

Cited By ~ 11

Author(s):

Busra Mammadov ◽

Melike Sever ◽

Mevhibe Gecer ◽

Fatih Zor ◽

Sinan Ozturk ◽

...

Keyword(s):

Sciatic Nerve ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Bioactive Peptide ◽

Peripheral Nerve Injuries ◽

Nerve Injuries ◽

Mimetic Peptide ◽

The Usa ◽

Sciatic Nerve Regeneration

Bioactive peptide gels enhance the regeneration of peripheral nerve injuries, which affect 20 million patients in the USA.

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Growth factor enhancement of peripheral nerve regeneration through a novel synthetic hydrogel tube

Journal of Neurosurgery ◽

10.3171/jns.2003.99.3.0555 ◽

2003 ◽

Vol 99 (3) ◽

pp. 555-565 ◽

Cited By ~ 141

Author(s):

Rajiv Midha ◽

Catherine A. Munro ◽

Paul D. Dalton ◽

Charles H. Tator ◽

Molly S. Shoichet

Keyword(s):

Growth Factors ◽

Growth Factor ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Collagen Gel ◽

Nerve Injuries ◽

Content Type ◽

Synthetic Hydrogel ◽

Fine Print

Object. The authors' long-term goal is repair of peripheral nerve injuries by using synthetic nerve guidance devices that improve both regeneration and functional outcome relative to an autograft. They report the in vitro processing and in vivo application of synthetic hydrogel tubes that are filled with collagen gel impregnated with growth factors. Methods. Poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) (PHEMA-MMA) porous 12-mm-long tubes with an inner diameter of 1.3 mm and an outer diameter of 1.8 mm were used to repair surgically created 10-mm gaps in the rat sciatic nerve. The inner lumen of the tubes was filled with collagen matrix alone or matrix supplemented with either neurotropin-3 at 1 µg/ml, brain-derived neurotrophic factor at 1 µg/ml, or acidic fibroblast growth factor (FGF-1) at 1 or 10 µg/ml. Nerve regeneration through the growth factor—enhanced tubes was assessed at 8 weeks after repair by histomorphometric analysis at the midgraft level and in the nerve distal to the tube repair. The tubes were biostable and biocompatible, and supported nerve regeneration in more than 90% of cases. Nerve regeneration was improved in tubes in which growth factors were added, compared with empty tubes and those containing collagen gel alone (negative controls). Tubes filled with 10 µg/ml of FGF-1 dispersed in collagen demonstrated regeneration comparable to autografts (positive controls) and showed significantly better regeneration than the other groups. Conclusions. The PHEMA-MMA tubes augmented with FGF-1 in their lumens appear to be a promising alternative to autografts for repair of nerve injuries. Studies are in progress to assess the long-term biocompatibility of these implants and to enhance regeneration further.

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