135 Purification and characterization of human placental-derived exosomes from maternal serum using size exclusion chromatography

2021 ◽  
Vol 224 (2) ◽  
pp. S92-S93
Author(s):  
Kendra Sylvester ◽  
Callie Reeder ◽  
Matthew W. Becker ◽  
Fatima Zahra Aly ◽  
Clive Wasserfall ◽  
...  
Keyword(s):  
Size Exclusion Chromatography ◽  
Maternal Serum ◽  
Size Exclusion ◽  
Purification And Characterization ◽  
Exclusion Chromatography

scholarly journals The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation

Viruses ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2272
Author(s):  
Hussein Kaddour ◽  
Malik Tranquille ◽  
Chioma M. Okeoma
Keyword(s):  
Size Exclusion Chromatography ◽  
Extracellular Vesicles ◽  
Cell Types ◽  
Size Exclusion ◽  
Target Cells ◽  
Purification And Characterization ◽  
Extracellular Milieu ◽  
Exclusion Chromatography ◽  
Single Vesicle

Extracellular vesicles (EVs) are cell-derived membranous particles secreted by all cell types (including virus infected and uninfected cells) into the extracellular milieu. EVs carry, protect, and transport a wide array of bioactive cargoes to recipient/target cells. EVs regulate physiological and pathophysiological processes in recipient cells and are important in therapeutics/drug delivery. Despite these great attributes of EVs, an efficient protocol for EV separation from biofluids is lacking. Numerous techniques have been adapted for the separation of EVs with size exclusion chromatography (SEC)-based methods being the most promising. Here, we review the SEC protocols used for EV separation, and discuss opportunities for significant improvements, such as the development of novel particle purification liquid chromatography (PPLC) system capable of tandem purification and characterization of biological and synthetic particles with near-single vesicle resolution. Finally, we identify future perspectives and current issues to make PPLC a tool capable of providing a unified, automated, adaptable, yet simple and affordable particle separation resource.

Purification and Characterization of Microbial Hyaluronic Acid by Solvent Precipitation and Size-Exclusion Chromatography

2009 ◽  
Vol 44 (4) ◽  
pp. 906-923 ◽  
Author(s):  
Anayla S. Sousa ◽  
Artemízia P. Guimarães ◽  
Caroline V. Gonçalves ◽  
Ivanildo J. Silva ◽  
Celio L. Cavalcante ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Purification And Characterization ◽  
Exclusion Chromatography

scholarly journals The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation

2021 ◽  
Author(s):  
Hussein Kaddour ◽  
Malik Tranquille ◽  
Chioma M. Okeoma
Keyword(s):  
Size Exclusion Chromatography ◽  
Extracellular Vesicles ◽  
Cell Types ◽  
Size Exclusion ◽  
Target Cells ◽  
Purification And Characterization ◽  
Extracellular Milieu ◽  
Exclusion Chromatography ◽  
Single Vesicle

Extracellular vesicles (EVs) are cell-derived membranous particles secreted by all cell types into the extracellular milieu. EVs carry, protect, and transport a wide array of bioactive cargoes to recipient/target cells. EVs regulate physiological and pathophysiological processes in recipient cells and are important in therapeutics/drug delivery. Despite these great attributes of EVs, an efficient protocol for EV separation from biofluids is lacking. Numerous techniques have been adapted for the separation of EVs with size exclusion chromatography (SEC)-based methods being the most promising. Here, we review the SEC protocols used for EV separation, and discuss opportunities for significant improvements, such as the development of novel particle purification liquid chromatography (PPLC) system capable of tandem purification and characterization of biological and synthetic particles with near-single vesicle resolution. Finally, we identify future perspectives and current issues to make PPLC a tool capable of providing a unified, automated, adaptable, yet simple and affordable particle separation resource.

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