Potential of microalgae Parachlorella kessleri Bh-2 as bioremediation agent of heavy metals cadmium and chromium

Algae Spirogyra peipingensis is a group of green algae from the order Zygnematales. Spirogyra algae serves as a bioremediation agent. In addition Spirogyra algae has great potential in reducing toxin levels in cigarette smoke. Because the biomass of alga Spirogyra has an irregular shaped surface texture and consists of amino acid, amide, carboxylic, hydroxyl, and carbonyl group that can bind various heavy metals and other harmful compounds. Alga Spirogyra peipingensis samples used are algae from Bone, South Sulawesi. Prior to the trial, the first pretreatment and processed to become powder. Algae powder was then applied to cigarette filters and tested on several respondents. Results showed that there was a significant decrease tar, nicotine and carbon monoxide levels in cigarette smoke by using Spirogyra peipingensis algae but the most significant decrease of carbon monoxide, while tar and nicotine decreased even though not as high as carbon monoxide.

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THE POTENCY OF COPPER-RESISTANT BACTERIA Cupriavidus sp. IrC4 ISOLATED FROM INDUSTRIAL WASTEWATER TREATMENT PLANT IN RUNGKUT-SURABAYA AS A BIOREMEDIATION AGENT FOR HEAVY METALS

KnE Life Sciences ◽

10.18502/kls.v2i1.179 ◽

2015 ◽

Vol 2 (1) ◽

pp. 375

Author(s):

Wahyu Irawati ◽

Triwibowo Yuwono ◽

Joedoro Soedarsono ◽

Hari Hartiko

Keyword(s):

Heavy Metals ◽

Wastewater Treatment ◽

Wastewater Treatment Plant ◽

Industrial Wastewater ◽

Treatment Plant ◽

Dry Weight ◽

Resistant Bacteria ◽

Industrial Wastewater Treatment ◽

Bioremediation Agent ◽

Copper Lead

Cupriavidus sp. IrC4 is a copper-resistant bacteria isolated from activated sludge in an Industrial Wastewater Treatment Plant in Rungkut-Surabaya, Indonesia. The purpose of this research was to study the potency of Cupriavidus sp. IrC4 as a bioremediation agent for copper, lead, mercury, and cadmium. Resistance of Cupriavidus sp. IrC4 to heavy metals were determined by measuring the minimum inhibitory concentration (MIC). Accumulation of copper, cadmium, and lead were determined by Atomic Absorption Spectrophotometer. Cupriavidus sp. IrC4 showed multiple resistance to heavy metals. The MICs of Cupriavidus sp. IrC4 to copper, lead, mercury, and cadmium were 16 mM, 15 mM, 6 mM, and 5 mM, respectively. The growth of Cupriavidus sp. IrC4 was inhibited by the addition of CuSO4 in the medium. The bacteria survived in the presence of high copper concentration as shown by the extension of the lag phase up to 36 hours. The analysis demonstrated that the copper resistance of the bacteria was facilitated through the accumulation of copper. Cupriavidus sp. IrC4 accumulated up to 367.78 and 260.01 mg/gram dry weight of cells of copper and lead, respectively. The bacteria demonstrated growth in the medium containing the mixture of 0.5 mM copper, lead, cadmium and accumulated those heavy metals up to 0.14, 24.74, and 12.49 mg/g dry weight of cells, respectively. The high resistance and capability of Cupriavidus sp. IrC4 to accumulate heavy metals can be exploited in bioremediation process for removing heavy metals from industrial sewage. Keywords: Accumulation, copper, Cupriavidus sp. IrC4,heavy metals, resistance.

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The effectivity of Biduri combined with indigenous bacteria in mercury absorption

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/905/1/012130 ◽

2021 ◽

Vol 905 (1) ◽

pp. 012130

Author(s):

Sutami ◽

Purwanto ◽

R Rosariastuti

Keyword(s):

Heavy Metals ◽

Soil Degradation ◽

Environmental Problems ◽

Indigenous Bacteria ◽

Randomized Design ◽

Heavy Metals Pollution ◽

Completely Randomized Design ◽

Soil Hg ◽

Hyperaccumulator Plant ◽

Bioremediation Agent

Abstract Heavy metals pollution, especially Mercury (Hg), is one of the most serious environmental problems. The presence of excessive Hg will cause soil degradation and threaten the life of the ecosystem, for that remediation is necessary. Biduri is known to be able to absorb heavy metals, but there is no research on the ability of Biduri in absorb Hg. The use of indigenous bacteria is expected to increase the absorption of Mercury by Biduri. The purpose of this study was to determine the potential of Biduri combined with indigenous bacteria and Agrobacterium sp I37 in absorbing of Hg in the soil. The experimental was designed as factorial with completely randomized design, consisting of 2 factors namely Bioremediation agent (A0: without bioremediation agent, A1: indigenous bacteria, A3: Agrobacterium sp I37) and Hg dosage (D0: without Hg, D1: Hg 0.3 µg.g-1, D2: Hg 0.51 µg.g-1). The results showed that the combination of Biduri with indigenous bacteria + 0.3 µg.g-1 Hg shows highest absorption of Hg at 57.19 µg (99.24% higher than control) and reduce soil Hg levels by 0.09 µg.g-1. Biduri is a hyperaccumulator plant because it is able to absorb more than 10 µg.g-1 of mercury.

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Indigenous multiresistant bacteria of Cupriavidus pauculus IrC4 isolated from Indonesia as a heavy metal bioremediation agent

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d220641 ◽

2021 ◽

Vol 22 (6) ◽

Author(s):

Irawati Wahyu ◽

STEVANUS ERICK WINOTO ◽

LUCIA KUSUMAWATI ◽

REINHARD PINONTOAN

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Bacterial Resistance ◽

Resistant Bacteria ◽

Lag Phase ◽

Lead And Cadmium ◽

Multiresistant Bacteria ◽

Cupriavidus Pauculus ◽

Bioremediation Agent ◽

Metal Bioremediation

Abstract. Irawati W, Winoto SE, Kusumawati L, Pinontoan R. 2021. Indigenous multiresistant bacteria of Cupriavidus pauculus IrC4 isolated from Indonesia as a heavy metal bioremediation agent. Biodiversitas 22: 3349-3355. Heavy metal pollution is a serious environmental problem because it endangers humans, animals, and plants. Bioremediation of heavy metals using bacteria is an effective method to remove heavy metals. Cupriavidus pauculus IrC4 is an indigenous multi-resistant bacteria isolated from Indonesia. This study aims to determine the growth of this strain in a medium containing cadmium, mercury, lead, copper, and its ability to accumulate heavy metal. Bacterial resistance was observed by cultivating bacteria on a Luria Bertani medium containing various concentrations of heavy metals. Heavy metal accumulation was measured using atomic absorption spectrophotometer. The study showed that this strain could grow in a solid medium containing 5 mM cadmium, 13 mM lead, and 4 mM mercury, also in 0.5 mM of the heavy metal mixture. A high concentration of heavy metals resulted in lag phase elongation and logarithmic growth phase inhibition. C. pauculus IrC4 could accumulate copper, lead, and cadmium and lead up to 371.42 mg, 254.4 mg, 5.8 mg heavy metals per gram of dry weight of cells, respectively. In conclusion, this strain is a promising bacterium for use as a heavy metal bioremediation agent.

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Evaluation of the Effectiveness of Live Synergistic Bacteria as a Bioremediation agent for selected Heavy Metals in Soils in Apapa-Lagos

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2016.506.048 ◽

2016 ◽

Vol 5 (6) ◽

pp. 413-421

Author(s):

G.I. Oyet ◽

P. Audu

Keyword(s):

Heavy Metals ◽

Heavy Metals In Soils ◽

Bioremediation Agent

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POTENSI PENURUNAN KONSENTRASI LOGAM BERAT TEMBAGA (CU) DAN PERTUMBUHAN MIKROALGA SPIRULINA PLANTESIS PADA MEDIA KULTUR

Jurnal Akuakultur Rawa Indonesia ◽

10.36706/jari.v6i1.7152 ◽

2018 ◽

Vol 6 (1) ◽

pp. 83-93

Author(s):

Mochamad Rendy Sety0 Budi ◽

Boedi Setya Rahardja ◽

Endang Dewi Masithah

Keyword(s):

Water Pollution ◽

Heavy Metals ◽

Heavy Metal ◽

Experimental Method ◽

Spirulina Platensis ◽

Living Things ◽

Randomized Design ◽

Environmental Component ◽

Completely Randomized Design ◽

Bioremediation Agent

ABSTRACT Water is an important environmental component for life. Heavy metal water pollution comes from many industries. Heavy Metals Copper (Cu) is one of several other heavy metals that are harmful to living things. One way to anticipate the increased pollution of heavy metals Copper (Cu) in waters is bioremediation using microalgae. This study aims to determine the ability of Spirulina plantesis in absorbing heavy metals Copper (Cu) and to determine the influence of heavy metal Copper (Cu) on the growth of Spirulina platensis. This study used an experimental method with Completely Randomized Design (RAL) consisting of four treatments and five replicates, namely A (S. platensis 0 ppm), B (S. platensis 1 ppm), C (S. platensis 3 ppm), D (S. Platensis 5 ppm). The results showed that Spirulina platensis was able to absorb heavy metal of Copper (Cu) so that it can be used as a heavy metal bioremediation agent. On treatment B (1 ppm) absorption of 87,719%, C (3 ppm) equal to 97,886% and D (5ppm) equal to 95,872 % Growth with the addition of Cu affects Spirulina platensis growthKeywords: Bioremediation, Spirulina platensis, Copper, Growth

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Sampling and analysis of the air-water interface with SEM and EDS of microlayers

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100157000 ◽

1989 ◽

Vol 47 ◽

pp. 1004-1005

Author(s):

Randall W. Smith ◽

John Dash

Keyword(s):

Heavy Metals ◽

Surface Microlayer ◽

Surface Films ◽

Water Interface ◽

Physical Processes ◽

Infrared Spectroscopic Analysis ◽

Eds Analysis ◽

Air Water Interface ◽

Significant Area ◽

Bulk Chemical

The structure of the air-water interface forms a boundary layer that involves biological ,chemical geological and physical processes in its formation. Freshwater and sea surface microlayers form at the air-water interface and include a diverse assemblage of organic matter, detritus, microorganisms, plankton and heavy metals. The sampling of microlayers and the examination of components is presently a significant area of study because of the input of anthropogenic materials and their accumulation at the air-water interface. The neustonic organisms present in this environment may be sensitive to the toxic components of these inputs. Hardy reports that over 20 different methods have been developed for sampling of microlayers, primarily for bulk chemical analysis. We report here the examination of microlayer films for the documentation of structure and composition.Baier and Gucinski reported the use of Langmuir-Blogett films obtained on germanium prisms for infrared spectroscopic analysis (IR-ATR) of components. The sampling of microlayers has been done by collecting fi1ms on glass plates and teflon drums, We found that microlayers could be collected on 11 mm glass cover slips by pulling a Langmuir-Blogett film from a surface microlayer. Comparative collections were made on methylcel1ulose filter pads. The films could be air-dried or preserved in Lugol's Iodine Several slicks or surface films were sampled in September, 1987 in Chesapeake Bay, Maryland and in August, 1988 in Sequim Bay, Washington, For glass coverslips the films were air-dried, mounted on SEM pegs, ringed with colloidal silver, and sputter coated with Au-Pd, The Langmuir-Blogett film technique maintained the structure of the microlayer intact for examination, SEM observation and EDS analysis were then used to determine organisms and relative concentrations of heavy metals, using a Link AN 10000 EDS system with an ISI SS40 SEM unit. Typical heavy microlayer films are shown in Figure 3.

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