scholarly journals POTENSI PENURUNAN KONSENTRASI LOGAM BERAT TEMBAGA (CU) DAN PERTUMBUHAN MIKROALGA SPIRULINA PLANTESIS PADA MEDIA KULTUR

2018 ◽  
Vol 6 (1) ◽  
pp. 83-93
Author(s):  
Mochamad Rendy Sety0 Budi ◽  
Boedi Setya Rahardja ◽  
Endang Dewi Masithah
Keyword(s):  
Water Pollution ◽  
Heavy Metals ◽  
Heavy Metal ◽  
Experimental Method ◽  
Spirulina Platensis ◽  
Living Things ◽  
Randomized Design ◽  
Environmental Component ◽  
Completely Randomized Design ◽  
Bioremediation Agent

ABSTRACT            Water is an important environmental component for life. Heavy metal water pollution comes from many industries. Heavy Metals Copper (Cu) is one of several other heavy metals that are harmful to living things. One way to anticipate the increased pollution of heavy metals Copper (Cu) in waters is bioremediation using microalgae. This study aims to determine the ability of Spirulina plantesis in absorbing heavy metals Copper (Cu) and to determine the influence of heavy metal Copper (Cu) on the growth of Spirulina platensis. This study used an experimental method with Completely Randomized Design (RAL) consisting of four treatments and five replicates, namely A (S. platensis 0 ppm), B (S. platensis 1 ppm), C (S. platensis 3 ppm), D (S. Platensis 5 ppm). The results showed that Spirulina platensis was able to absorb heavy metal of Copper (Cu) so that it can be used as a heavy metal bioremediation agent. On treatment B (1 ppm) absorption of 87,719%, C (3 ppm) equal to 97,886% and D (5ppm) equal to 95,872 % Growth with the addition of Cu affects Spirulina platensis growthKeywords: Bioremediation, Spirulina platensis, Copper, Growth

scholarly journals Korelasi Antara Daya Serap Gracilaria sp. Terhadap Konsentrasi Logam Berat Cu di Media Pemeliharaan

2021 ◽  
Vol 10 (3) ◽  
pp. 363-368
Author(s):  
Regina Amanda ◽  
Ria Azizah Tri Nuraini ◽  
Endang Supriyantini
Keyword(s):  
Water Pollution ◽  
Heavy Metals ◽  
Heavy Metal ◽  
Weight Gain ◽  
Metal Content ◽  
Absorption Capacity ◽  
Control Treatment ◽  
Living Things ◽  
Randomized Design ◽  
Completely Randomized Design

Pencemaran air terhadap lingkungan dapat menyebabkan dampak membahayakan yang dapat dirasakan oleh makhluk hidup. Salah satu logam berat yang sering mencemari air adalah logam berat tembaga (Cu). Tujuan penelitian ini untuk mengetahui kemampuan daya serap rumput laut Gracilaria sp. terhadap logam berat Cu dan pertumbuhannya. Penelitian ini menggunakan metode eksperimental laboratoris dengan Rancangan Acak Lengkap (RAL) dengan menggunakan 4 perlakuan penambahan konsentrasi Cu, yaitu kontrol (0,54 ppm, sesuai dengan konsentrasi Cu pada air laut), 3 ppm, 6 ppm, 9 ppm dengan 3 kali pengulangan. Pengukuran kandungan logam Cu terhadap Gracilaria sp. dengan menggunakan Atomic Absorpsion Spektofotometri (AAS). Hasil penelitian menunjukkan bahwa penyerapan logam berat Cu tertinggi terdapat pada konsentrasi 9 ppm, yaitu sebesar 3,63 ppm dan terendah pada kontrol yaitu sebesar 0,27 ppm.. Pertumbuhan Gracilaria sp. tertinggi terdapat pada perlakuan kontrol dengan pertambahan berat sebesar 209,2 gram dan  terendah pada konsentrasi 9 ppm, yaitu sebesar 162,9 gram. Semakin tinggi konsentrasi Cu yang diberikan maka semakin rendah pertumbuhan Gracilaria sp. Water pollution to the environment can cause dangerous impacts that can be felt by living things. One of the heavy metals that often pollutes water is the heavy metal copper (Cu). The purpose of this study was to determine the absorption capacity of Gracilaria sp. to the heavy metal Cu and its growth. This study used a laboratory experimental method with a completely randomized design (RAL) using 4 additional treatments of Cu concentration, namely control (0.54 ppm, according to the Cu concentration in seawater), 3 ppm, 6 ppm, 9 ppm with 3 repetitions. Measurement of Cu metal content against Gracilaria sp. by using Atomic Absorption Spectophotometry (AAS). The results showed that the highest absorption of heavy metal Cu was at a concentration of 9 ppm, which was 3.63 ppm and the lowest was 0,27 ppm in control.. The Growth of Gracilaria sp. The highest was found in the control treatment with a weight gain of 209.2 grams and the lowest was at a concentration of 9 ppm, which was 162.9 grams. The higher the concentration of Cu given, the lower the growth of Gracilaria sp. 

scholarly journals Evaluation of Phytoremediation Potential of Castor Cultivars for Heavy Metals from Soil

2019 ◽  
Vol 37 ◽  
Author(s):  
M.J. KHAN ◽  
N. AHMED ◽  
W. HASSAN ◽  
T. SABA ◽  
S. KHAN ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Contaminated Soils ◽  
Metal Uptake ◽  
Plant Biomass ◽  
Ricinus Communis ◽  
Contaminated Sites ◽  
Heavy Metal Uptake ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACT: Phytoremediation is a useful tool to restore heavy metals contaminated soils. This study was carried out to test two castor (Ricinus communis) cultivars [Local and DS-30] for phytoextraction of heavy metals from the soil spiked by known concentrations of seven metals (Cu, Cr, Fe, Mn, Ni, Pb and Zn). A pot experiment was laid out by using a completely randomized design. Soil and plant samples were analyzed at 100 days after planting. The data on heavy metal uptake by plant tissues (roots, leaves and shoots) of the two castor cultivars suggested that a considerable amount of metals (Fe = 27.18 mg L-1; Cu = 5.06 mg L-1; Cr = 2.95 mg L-1; Mn = 0.22 mg L-1; Ni = 4.66 mg L-1; Pb = 3.33 mg L-1; Zn = 15.04 mg L-1) was accumulated in the plant biomass. The soil heavy metal content at the end of experiment significantly decreased with both cultivars, resulting in improved soil quality. Therefore, it is concluded that both castor cultivars, Local and DS-30, can be used for phytoremediation of heavy metal-contaminated sites.

scholarly journals PENGARUH LOGAM BERAT TIMBAL TERHADAP PERTUMBUHAN ALGA Halimeda opuntia YANG DIKULTUR DALAM WADAH TERKONTROL

2020 ◽  
Vol 8 (3) ◽  
pp. 17
Author(s):  
Kristi Aji Sugiarto ◽  
Desy MH Mantiri ◽  
Nickson J Kawung ◽  
Rizald M Rompas ◽  
Sandra O Tilaar ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Content ◽  
Research Method ◽  
Lead Content ◽  
Randomized Design ◽  
Algae Growth ◽  
Completely Randomized Design ◽  
Over Time ◽  
Bioremediation Agent

Heavy metal is a very dangerous pollutant waste for it cannot be destroyed (non-degradable) and over time it will accumulate in the ocean. This study aims to determine the lead content in sediment, water and algal thallus as well as to determine the algae growth that has been treated with lead. The research method used was a completely randomized design (CRD) which consisted of 3 treatments, using different treatments for each sample. The highest metal content obtained in the sediment was 5,124 ppm, in the water was 0.120 ppm, and in the algae thallus was 4,816 ppm respectively. The results showed that Halimeda opuntia algae can grow even in an environment contaminated with lead heavy metal and can become a bioremediation agent by absorbing lead heavy metal. Keywords: Halimeda opuntia, Lead Heavy Metal, Algae Culture, Bioremediation             Logam berat merupakan salah satu limbah pencemar yang sangat berbahaya karena tidak dapat dihancurkan (non degradable) dan dapat terakumulasi di perairan laut. Penelitian ini bertujuan untuk mengetahui kandungan timbal pada sedimen, air dan thallus alga yang dikultur serta mengetahui pertumbuhan alga yang telah diberi perlakuan timbal. Metode penelitian mengunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan, dengan menggunakan perlakuan yang berbeda pada tiap sampel dan kontrol. Kandungan logam tertinggi yang didapatkan pada sedimen yaitu sebesar 5,124 ppm, pada air sebesar 0,120 ppm, dan pada thallus alga sebesar 4,816 ppm. Hasil yang diperoleh yaitu alga Halimeda opuntia dapat bertumbuh walaupun dalam lingkungan yang tercemar logam berat timbal serta mampu menjadi agen bioremediasi dengan menyerap logam berat timbal. Kata kunci: Halimeda opuntia, Logam BeratTimbal, Kultur Alga, Bioremediasi

scholarly journals The effectivity of Biduri combined with indigenous bacteria in mercury absorption

2021 ◽  
Vol 905 (1) ◽  
pp. 012130
Author(s):  
Sutami ◽  
Purwanto ◽  
R Rosariastuti
Keyword(s):  
Heavy Metals ◽  
Soil Degradation ◽  
Environmental Problems ◽  
Indigenous Bacteria ◽  
Randomized Design ◽  
Heavy Metals Pollution ◽  
Completely Randomized Design ◽  
Soil Hg ◽  
Hyperaccumulator Plant ◽  
Bioremediation Agent

Abstract Heavy metals pollution, especially Mercury (Hg), is one of the most serious environmental problems. The presence of excessive Hg will cause soil degradation and threaten the life of the ecosystem, for that remediation is necessary. Biduri is known to be able to absorb heavy metals, but there is no research on the ability of Biduri in absorb Hg. The use of indigenous bacteria is expected to increase the absorption of Mercury by Biduri. The purpose of this study was to determine the potential of Biduri combined with indigenous bacteria and Agrobacterium sp I37 in absorbing of Hg in the soil. The experimental was designed as factorial with completely randomized design, consisting of 2 factors namely Bioremediation agent (A0: without bioremediation agent, A1: indigenous bacteria, A3: Agrobacterium sp I37) and Hg dosage (D0: without Hg, D1: Hg 0.3 µg.g-1, D2: Hg 0.51 µg.g-1). The results showed that the combination of Biduri with indigenous bacteria + 0.3 µg.g-1 Hg shows highest absorption of Hg at 57.19 µg (99.24% higher than control) and reduce soil Hg levels by 0.09 µg.g-1. Biduri is a hyperaccumulator plant because it is able to absorb more than 10 µg.g-1 of mercury.

scholarly journals EFFECT OF NITRATE AND PHOSPATE CONCENTRATION ON Spirulina platensis WITH INDOOR SCALE

2021 ◽  
Vol 4 (1) ◽  
pp. 29-35
Author(s):  
Safira Mutia ◽  
Syahril Nedi ◽  
Elizal Elizal
Keyword(s):  
Water Quality ◽  
Fatty Acids ◽  
Experimental Method ◽  
Spirulina Platensis ◽  
Quality Parameters ◽  
Nutritional Content ◽  
Maximum Biomass ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
C 30

One of the microalgae that are widely used in the industrial world is Spirulina platensis which has high nutritional content of protein, fatty acids, vitamins and antioxidants. In its growth, this microalgae requires media and the addition of macronutrients nitrate and phosphate which greatly affect the growth and productivity of S. platensis biomass. This study aims to determine the effect of nitrate and phosphate concentrations on S. platensis biomass on an indoor scale and to determine the optimal nitrate and phosphate concentrations to achieve maximum biomass. This research was conducted in August 2020 as an experimental method, with culturing S. platensis with the addition of different concentrations of nitrate and phosphate. The design used in this study was a Completely Randomized Design consisting of 5 treatments that are 0 ppm, 10 ppm, 20 ppm, 30 ppm, and 40 ppm, each treatment with 3 repetitions. The source of nitrate and phosphate used is NPK. Water quality parameters during culture with temperatures ranging from 29-30oC, salinity 25-26 ppt, and pH 8. The optimal concentrations of nitrate and phosphate to achieve the maximum biomass is in treatment C (30 ppm) with a total of biomass 0,43 g. Whereas in treatment D (40 ppm) the biomass did not show a significant increase because the dose was too high with a total of biomass only 0,299 g

scholarly journals PENGGUNAAN COAL FLY ASH (CFA) DAN TANDAN KOSONG KELAPA SAWIT (TKKS) UNTUK PERBAIKAN TANAH ULTISOL DAN SERAPAN LOGAM BERAT

BUANA SAINS ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 21-28
Author(s):  
Aryo Candra Nugroho ◽  
Amir Hamzah ◽  
Hesti Triana Soelistriari
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Fly Ash ◽  
Metal Content ◽  
Main Type ◽  
Coal Fly Ash ◽  
Land Area ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Very High

Ultisol is the main type of land in Kalimantan, Sumatra, Papua and Sulawesi, with an area of almost 45.8 million ha or 25% of the land area of Indonesia. Ultisol has a relatively high acidity (average pH 4.5), high Al saturation, reaching 60%. The efforts to increase the fertility of ultisol soils such as the addition of organic materials such as Empty Oil Palm Bunches (OPEFB) and CFA (Coal Fly Ash) or Coal Fly Ash (CFA) improve the physical properties of Ultisol soil. Use of CFA that exceeds the limit can cause pollution especially copper (Cu) and lead (Pb). One way to recover soil from heavy metal contaminants with Phytoremediation technology, mustard (Brassica juncea L.) is one of the plants that easily absorb heavy metals so that it can be used as a Phytoremediation agent. The aims of this research to determine the use of CFA and OPEFB to fix up the ultisol soil and heavy metal content absorbed by plants. This study used Completely Randomized Design with 3 replications. The results showed that using of CFA and OPEFB can increase the available P so that it can increase the growth of mustard plants and absorption of heavy metals. By using the dozes of 54.54g CFA and 18.18g OPEFB (C3K1) can increase P- available at 181.67 m kg-1 . The uptake of heavy metals Cu and Pb in mustard plants is very high. The highest uptake in Cu reached 192.03 mg kg-1 , whereas in Pb it was 80 mg kg-1 . This number is still very high, so it is dangerous if consumed.

scholarly journals The Growth and Antioxidative Responses of Sonchus oleraceus (Linnaeus, 1753) Under Cu(II), Pb(II), Cd(II) and Cr(VI) Stress Condition

2017 ◽  
Vol 3 (4) ◽  
pp. 157
Author(s):  
Nur Hidayaturrohmah ◽  
Sri Kasmiyati ◽  
Sucahyo . ◽  
Zane Vincēviča-Gaile
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Dry Weight ◽  
Sonchus Oleraceus ◽  
Antioxidative Responses ◽  
Randomized Design ◽  
Growing Medium ◽  
Metal Treatment ◽  
Completely Randomized Design ◽  
The Impact

<p class="Els-Abstract-text">Plants growing in soil containing heavy metal  polutan such as chromium (Cr), lead (Pb), cadmium (Cd) and copper (Cu)  will be stunted, and  increase production of Reactive Oxygen Species (ROS). In dealing with the excess amount of ROS, plants have an enzymatic defense system, using superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxide (APX). The aim of this study was to determine and analyze the <em>Sonchus oleraceus</em> (Linnaeus, 1753) plant response to heavy metals stress, seen from the growth and antioxidative defense enzymatically. Research carried out using a Completely Randomized Design (CRD) with four treatments and five replicates. The metal treatment was 10 mg · L<sup>–1</sup>. The presence of heavy metals in the growing medium significantly decreased the plant height and leaf area, so the impact is on the weight of wet and dry weight. The metal treatments of Cr, Cd, Pb and Cu increase the activity of SOD and APX enzymes but decrease the activity of the CAT enzyme. Chromium is a metal that has a significant influence on the growth and activity of SOD, APX, and CAT enzymes in <em>S. oleraceus</em>.</p><div><p class="Els-keywords"><strong>Keywords:</strong> antioxidative responses; growth; heavy metal; <em>Sonchus oleraceus; </em>toxicity.</p></div>

scholarly journals BIOREMEDIASI Pb OLEH BAKTERI INDIGEN LIMBAH CAIR AGAR

2019 ◽  
Vol 1 (2) ◽  
pp. 51-58
Author(s):  
Senja Ikerismawati
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Research Design ◽  
Bacillus Pumilus ◽  
Liquid Waste ◽  
Bacterial Isolates ◽  
Indigenous Bacteria ◽  
Randomized Design ◽  
Treatment Data ◽  
Completely Randomized Design

Bacteria isolated from ad environment contaminated with heavy metals are very potential as heavy metal bioremediation agents called indigenous bacteria. The purpose of this study was to isolate and identify indigenic bacteria that have the potential as Pb bioremediation agents in agar liquid waste and to analyze the ability of indigenic bacteria in reducing Pb. The research design used Completely Randomized Design (RAL) two factorial with variation of bacteria and eight days of treatment. Data were analyzed using two-way ANOVA with Duncan's advanced test. The results showed that there were eight isolates resulting from the isolation of agar liquid waste. The isolates of liquid waste indigen bacteria so that the most potential in reducing Pb were isolated H, E and F. The three isolates were able to reduce Pb in sterile agar liquid waste by 82.6%, 81.3% and 79.3% for eight days of treatment. The identification results using Microbact TM GNB 12A / B / E, 24 Identification Kits showed that H bacterial isolates were Bacillus alvei, E isolates were Bacillus pumilus species and F isolates were Bacillus lichenformis species

scholarly journals Studi Perbandingan Kemampuan Nannochloropsis sp. Dan Spirulina sp. Sebagai Agen Bioremediasi Terhadap Logam Berat Timbal (Pb) [Comparative Study Of Ability Nannochloropsis sp. And Spirulina sp. As Agent Bioremediation Of Heavy Metal Plumbum (Pb) ]

2019 ◽  
Vol 5 (2) ◽  
pp. 175
Author(s):  
Khilyatun Nisak, Boedi Setya Rahardja, Endang Dewi Masithah
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Water Bodies ◽  
Test Results ◽  
Chlorella Sp ◽  
Normality Test ◽  
Toxic Material ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Active Cluster

Abstract Microalgae species Nannochloropsis sp. can used as heavy metal biosorben because of it’s adsorption capability that caused by the active cluster which contained in that species (Sembiring et al., 2008). Chlorella sp. is one of phytoplankton species that has the bioaccumulation ability of heavy metals and easily cultivated (Arifin, 1997). Lead (Pb) is a mineral belonging to microelements, is a heavy metal and is a potentially toxic material. Water bodies that have been polluted by compounds or ions Pb because can lead to the death of aquatic biota, the number of Lead (Pb) present in water bodies exceeding the proper concentration (Palar, 2004). One way to anticipate the increasing heavy metal pollution in the water is to bioremediation. The research using experimentally, the research design used was completely randomized design (CRD) consisting of four treatments with five replications. The concentrations of heavy metals Plumbum (Pb) used is 0 ppm and 0.9 ppm. The main parameters in this study is the ability of bioremediation Plumbum (Pb) by Nannochloropsis sp. and Chlorella sp. SPSS analytics normality test results and test the ability of T 95% in Nannochloropsis sp. and Chlorella sp. in absorbing heavy metals Plumbum (Pb) concentrations of 0 ppm and 0.9 ppm indicate that the data is normal and the results obtained were significantly different / significant. While the analysis of SPSS test T on heavy metal absorption capability comparison Plumbum (Pb) concentration of 0 ppm and 0.9 ppm by Nannochloropsis sp. and Chlorella sp. showed that the results were not significantly different / non significant. On average results obtained, Nannochloropsis sp. have a higher capacity than Chlorella sp. in the bioremediation process of heavy metals Plumbum (Pb).

scholarly journals A New Ex Vivo Model to Evaluate the Hair Protective Effect of a Biomimetic Exopolysaccharide against Water Pollution

Cosmetics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 78
Author(s):  
Claire Tubia ◽  
Alfonso Fernández-Botello ◽  
Jan Dupont ◽  
Eni Gómez ◽  
Jérôme Desroches ◽  
...  
Keyword(s):  
Water Pollution ◽  
Heavy Metals ◽  
Heavy Metal ◽  
Adverse Effects ◽  
Inductively Coupled Plasma ◽  
Ex Vivo ◽  
Polluted Water ◽  
Ex Vivo Model ◽  
Fiber Damage ◽  
The Impact

As an external appendage, hair is exposed to multiple stresses of different origins such as particles and gases in air, or heavy metals and chemicals in water. So far, little research has addressed the impact of water pollution on hair. The present study describes a new ex vivo model that allowed us to document the adverse effects of water pollutants on the structure of hair proteins, as well as the protective potential of active cosmetic ingredients derived from a biomimetic exopolysaccharide (EPS). The impact of water pollution was evaluated on hair from a Caucasian donor repeatedly immersed in heavy metal-containing water. Heavy metal retention in and on hair was then quantified using Inductively Coupled Plasma Spectrometry (ICP/MS). The adverse effects of heavy metals on the internal structure of hair and its prevention by the EPS were assessed through measurement of keratin birefringence. Notably, the method allows the monitoring of the organization of keratin fibers and therefore the initial change on it in order to modulate the global damage in the hair. Results revealed an increasing amount of lead, cadmium and copper, following multiple exposures to polluted water. In parallel, the structure of keratin was also altered with exposures. However, heavy metal-induced keratin fiber damage could be prevented in the presence of the tested EPS, avoiding more drastic hair problems, such as lack of shine, or decrease in strength, due to damage accumulation.

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