scholarly journals The effectivity of Biduri combined with indigenous bacteria in mercury absorption

2021 ◽  
Vol 905 (1) ◽  
pp. 012130
Author(s):  
Sutami ◽  
Purwanto ◽  
R Rosariastuti
Keyword(s):  
Heavy Metals ◽  
Soil Degradation ◽  
Environmental Problems ◽  
Indigenous Bacteria ◽  
Randomized Design ◽  
Heavy Metals Pollution ◽  
Completely Randomized Design ◽  
Soil Hg ◽  
Hyperaccumulator Plant ◽  
Bioremediation Agent

Abstract Heavy metals pollution, especially Mercury (Hg), is one of the most serious environmental problems. The presence of excessive Hg will cause soil degradation and threaten the life of the ecosystem, for that remediation is necessary. Biduri is known to be able to absorb heavy metals, but there is no research on the ability of Biduri in absorb Hg. The use of indigenous bacteria is expected to increase the absorption of Mercury by Biduri. The purpose of this study was to determine the potential of Biduri combined with indigenous bacteria and Agrobacterium sp I37 in absorbing of Hg in the soil. The experimental was designed as factorial with completely randomized design, consisting of 2 factors namely Bioremediation agent (A0: without bioremediation agent, A1: indigenous bacteria, A3: Agrobacterium sp I37) and Hg dosage (D0: without Hg, D1: Hg 0.3 µg.g-1, D2: Hg 0.51 µg.g-1). The results showed that the combination of Biduri with indigenous bacteria + 0.3 µg.g-1 Hg shows highest absorption of Hg at 57.19 µg (99.24% higher than control) and reduce soil Hg levels by 0.09 µg.g-1. Biduri is a hyperaccumulator plant because it is able to absorb more than 10 µg.g-1 of mercury.

scholarly journals POTENSI PENURUNAN KONSENTRASI LOGAM BERAT TEMBAGA (CU) DAN PERTUMBUHAN MIKROALGA SPIRULINA PLANTESIS PADA MEDIA KULTUR

2018 ◽  
Vol 6 (1) ◽  
pp. 83-93
Author(s):  
Mochamad Rendy Sety0 Budi ◽  
Boedi Setya Rahardja ◽  
Endang Dewi Masithah
Keyword(s):  
Water Pollution ◽  
Heavy Metals ◽  
Heavy Metal ◽  
Experimental Method ◽  
Spirulina Platensis ◽  
Living Things ◽  
Randomized Design ◽  
Environmental Component ◽  
Completely Randomized Design ◽  
Bioremediation Agent

ABSTRACT            Water is an important environmental component for life. Heavy metal water pollution comes from many industries. Heavy Metals Copper (Cu) is one of several other heavy metals that are harmful to living things. One way to anticipate the increased pollution of heavy metals Copper (Cu) in waters is bioremediation using microalgae. This study aims to determine the ability of Spirulina plantesis in absorbing heavy metals Copper (Cu) and to determine the influence of heavy metal Copper (Cu) on the growth of Spirulina platensis. This study used an experimental method with Completely Randomized Design (RAL) consisting of four treatments and five replicates, namely A (S. platensis 0 ppm), B (S. platensis 1 ppm), C (S. platensis 3 ppm), D (S. Platensis 5 ppm). The results showed that Spirulina platensis was able to absorb heavy metal of Copper (Cu) so that it can be used as a heavy metal bioremediation agent. On treatment B (1 ppm) absorption of 87,719%, C (3 ppm) equal to 97,886% and D (5ppm) equal to 95,872 % Growth with the addition of Cu affects Spirulina platensis growthKeywords: Bioremediation, Spirulina platensis, Copper, Growth

scholarly journals BIOREMEDIASI Pb OLEH BAKTERI INDIGEN LIMBAH CAIR AGAR

2019 ◽  
Vol 1 (2) ◽  
pp. 51-58
Author(s):  
Senja Ikerismawati
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Research Design ◽  
Bacillus Pumilus ◽  
Liquid Waste ◽  
Bacterial Isolates ◽  
Indigenous Bacteria ◽  
Randomized Design ◽  
Treatment Data ◽  
Completely Randomized Design

Bacteria isolated from ad environment contaminated with heavy metals are very potential as heavy metal bioremediation agents called indigenous bacteria. The purpose of this study was to isolate and identify indigenic bacteria that have the potential as Pb bioremediation agents in agar liquid waste and to analyze the ability of indigenic bacteria in reducing Pb. The research design used Completely Randomized Design (RAL) two factorial with variation of bacteria and eight days of treatment. Data were analyzed using two-way ANOVA with Duncan's advanced test. The results showed that there were eight isolates resulting from the isolation of agar liquid waste. The isolates of liquid waste indigen bacteria so that the most potential in reducing Pb were isolated H, E and F. The three isolates were able to reduce Pb in sterile agar liquid waste by 82.6%, 81.3% and 79.3% for eight days of treatment. The identification results using Microbact TM GNB 12A / B / E, 24 Identification Kits showed that H bacterial isolates were Bacillus alvei, E isolates were Bacillus pumilus species and F isolates were Bacillus lichenformis species

scholarly journals Evaluation of Phytoremediation Potential of Castor Cultivars for Heavy Metals from Soil

2019 ◽  
Vol 37 ◽  
Author(s):  
M.J. KHAN ◽  
N. AHMED ◽  
W. HASSAN ◽  
T. SABA ◽  
S. KHAN ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Contaminated Soils ◽  
Metal Uptake ◽  
Plant Biomass ◽  
Ricinus Communis ◽  
Contaminated Sites ◽  
Heavy Metal Uptake ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACT: Phytoremediation is a useful tool to restore heavy metals contaminated soils. This study was carried out to test two castor (Ricinus communis) cultivars [Local and DS-30] for phytoextraction of heavy metals from the soil spiked by known concentrations of seven metals (Cu, Cr, Fe, Mn, Ni, Pb and Zn). A pot experiment was laid out by using a completely randomized design. Soil and plant samples were analyzed at 100 days after planting. The data on heavy metal uptake by plant tissues (roots, leaves and shoots) of the two castor cultivars suggested that a considerable amount of metals (Fe = 27.18 mg L-1; Cu = 5.06 mg L-1; Cr = 2.95 mg L-1; Mn = 0.22 mg L-1; Ni = 4.66 mg L-1; Pb = 3.33 mg L-1; Zn = 15.04 mg L-1) was accumulated in the plant biomass. The soil heavy metal content at the end of experiment significantly decreased with both cultivars, resulting in improved soil quality. Therefore, it is concluded that both castor cultivars, Local and DS-30, can be used for phytoremediation of heavy metal-contaminated sites.

scholarly journals PENGARUH LOGAM BERAT TIMBAL TERHADAP PERTUMBUHAN ALGA Halimeda opuntia YANG DIKULTUR DALAM WADAH TERKONTROL

2020 ◽  
Vol 8 (3) ◽  
pp. 17
Author(s):  
Kristi Aji Sugiarto ◽  
Desy MH Mantiri ◽  
Nickson J Kawung ◽  
Rizald M Rompas ◽  
Sandra O Tilaar ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Content ◽  
Research Method ◽  
Lead Content ◽  
Randomized Design ◽  
Algae Growth ◽  
Completely Randomized Design ◽  
Over Time ◽  
Bioremediation Agent

Heavy metal is a very dangerous pollutant waste for it cannot be destroyed (non-degradable) and over time it will accumulate in the ocean. This study aims to determine the lead content in sediment, water and algal thallus as well as to determine the algae growth that has been treated with lead. The research method used was a completely randomized design (CRD) which consisted of 3 treatments, using different treatments for each sample. The highest metal content obtained in the sediment was 5,124 ppm, in the water was 0.120 ppm, and in the algae thallus was 4,816 ppm respectively. The results showed that Halimeda opuntia algae can grow even in an environment contaminated with lead heavy metal and can become a bioremediation agent by absorbing lead heavy metal. Keywords: Halimeda opuntia, Lead Heavy Metal, Algae Culture, Bioremediation             Logam berat merupakan salah satu limbah pencemar yang sangat berbahaya karena tidak dapat dihancurkan (non degradable) dan dapat terakumulasi di perairan laut. Penelitian ini bertujuan untuk mengetahui kandungan timbal pada sedimen, air dan thallus alga yang dikultur serta mengetahui pertumbuhan alga yang telah diberi perlakuan timbal. Metode penelitian mengunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan, dengan menggunakan perlakuan yang berbeda pada tiap sampel dan kontrol. Kandungan logam tertinggi yang didapatkan pada sedimen yaitu sebesar 5,124 ppm, pada air sebesar 0,120 ppm, dan pada thallus alga sebesar 4,816 ppm. Hasil yang diperoleh yaitu alga Halimeda opuntia dapat bertumbuh walaupun dalam lingkungan yang tercemar logam berat timbal serta mampu menjadi agen bioremediasi dengan menyerap logam berat timbal. Kata kunci: Halimeda opuntia, Logam BeratTimbal, Kultur Alga, Bioremediasi

scholarly journals RESPON PERTUMBUHAN BIBIT API-API ( Avicennia marina ) TERHADAP CEKAMAN MERKURI (Hg)

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Muhammad Hafiz ◽  
Rosa Suryantini ◽  
Sarma Siahaan ◽  
Herlina Darwati
Keyword(s):  
Heavy Metals ◽  
Confidence Level ◽  
Biological Indicators ◽  
Avicennia Marina ◽  
Polluted Environment ◽  
Randomized Design ◽  
Mercury Stress ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Number Of Seeds

Api-api (Avicennia marina) has potential as the phytoremediation because it can live in a polluted environment.  Api-api can be used as biological indicators of the environment that was polluted by heavy metals. The purpose of this research was to measure the effect of growth (the increase in diameter and the number of leaves) of api-api seeds on mercury stress with a dose of 0.5 ml, 2 ml, and 3.5 ml. The research used an experimental method with a completely randomized design, which consists of 4 treatments and 5 repetitions. Each repetition consists of 1 plant, thus the number of seeds that have been observed was 20 plants. The results of the research showed that the mercury solution inhibited the increase in diameter and number of leaves with a confidence level of 95%. Keyword: Avicennia marina, mercury grip, seedling growth.

scholarly journals Influence of mycorrhizal fungi on phytoremediating potential and yield of sunflower in Cd and Pb polluted soils

2010 ◽  
Vol 55 (1) ◽  
pp. 17-28
Author(s):  
M.B. Adewole ◽  
O.O. Awotoye ◽  
M.O. Ohiembor ◽  
A.O. Salami
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizae ◽  
Glomus Intraradices ◽  
Polluted Soils ◽  
Pollution Tolerance ◽  
Randomized Design ◽  
Soil Solutions ◽  
Top Soil ◽  
Heavy Metals Pollution ◽  
Completely Randomized Design

The influence of mycorrhizal fungi in uptake of heavy metals, pollution response index and yield of sunflower in degraded soils were investigated. It was a greenhouse experiment with 2 arbuscular mycorrhizae (Glomus mosseae, Glomus intraradices) and a non-inoculation that served as control. The treatments were replicated 3 times in a completely randomized design. Each of the treatment consisted of 30 pots and each pot was filled with 5 kg by weight of dried top soil. Solutions of lead acetate and cadmium sulphate at variable levels of: 0, 250, 500, 750, 1000 mg kg-1 and 0, 20, 40, 60, 80 mg kg-1 respectively were used to pollute the soils. Increase in pollution-stressed conditions significantly (P<0.05) reduced the infection of sunflower roots, and the uptake of Pb and Cd in the dry root of sunflower was also significantly (P<0.05) reduced. Also, arbuscular mycorrhizae enhanced the root infection of sunflower, increased the pollution tolerance and consequently increased the yield of sunflower.

scholarly journals Heavy Metals Uptake of Salty Soils by Ornamental Sunflower, Using Cow Manure and Biosolids: A Case Study in Alborz city, Iran

2020 ◽  
Vol 13 ◽  
pp. 117862211989846 ◽  
Author(s):  
Ahmadreza Yazdanbakhsh ◽  
Seyed Nadali Alavi ◽  
Seyed Alireza Valadabadi ◽  
Fatemeh Karimi ◽  
Zainab Karimi
Keyword(s):  
Heavy Metals ◽  
Wastewater Treatment Plants ◽  
Environmental Pollutants ◽  
Organic Amendments ◽  
Heavy Metal Accumulation ◽  
Cow Manure ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Heavy Metals Uptake

Heavy metals are among the most critical environmental pollutants close to industrial areas. One example is the cultivated fields in the south of Alborz industrial city in Iran, which is irrigated by treated industrial wastewater. It is contaminated by heavy metals and irrigation with wastewater treatment plants effluent, which made it salty. In this study, the application of 2 amendments, biosolids and cow manure, in improving the heavy metal accumulation in the ornamental sunflower from these types of soils was investigated. A greenhouse experiment using a completely randomized design with 4 replications and applying cow manure and biosolids in 3 weight ratios (6%, 12%, 25%) was conducted to evaluate the efficiency of sunflower in removing Pb, Ni, and Zn from the soil. Adding the amendments increased the rate of germination by 50% to 176%. Although the simultaneous utilization of cow manure in high ratios with biosolids and cow manure with low biosolids decreased the sunflower survival, nonetheless, the simultaneous addition of these organic amendments could increase the survival rate in other treatments. Moreover, the plants’ biomass was increased by adding modifiers such as cow manure and biosolids. The results showed that in treatments with 2 modifiers, the remediation factor of Pb, Zn, and Ni has increased 83.7 to 95.5, 78.4 to 87.5, and 74.9 to 94.9, respectively, in comparison to the control one. Therefore, we conclude that adding biosolids and cow manure simultaneously could improve the ornamental sunflower ability to accumulate heavy metals.

scholarly journals Respons Hasil Padi dan Hara Tanah Sawah terhadap Bakteri Pelarut Fosfat dan Pemfiksasi Nitrogen

2021 ◽  
Vol 5 (2) ◽  
pp. 29-34
Author(s):  
Muhimmatul Husna ◽  
Sugiyanta Sugiyanta ◽  
Etty Pratiwi
Keyword(s):  
Paddy Field ◽  
Soil Degradation ◽  
Fertilizer Application ◽  
Rice Grain ◽  
Nitrogen Fixer ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Phosphate Solubilizing ◽  
Paddy Cultivation ◽  
Experimental Garden

The condition of unfertile paddy field due to excessive inorganic fertilizer application causing soil degradation. Fertilizing techniques using phosphate solubilizing  and nitrogen fixer bacteria are breakthroughs that should be developed in paddy cultivation to increase fertility soil and harvest of rice. This research aims to study on phosphate solubilizing and nitrogen fixer bacteria ability to increase the harvest of rice by increasing chemical and biological fertility of soil. The research was conducted in the greenhouse of Sawah Baru experimental garden, IPB University. The design used was a completely randomized design with one factor that was dosage of inorganic fertilizers (NPK) with seven levels that were control, NPK 100%, NPK 0% + bacteria, NPK 25% + bacteria, NPK 50% + bacteria, NPK 75% + bacteria and NPK 100% + bacteria. Each stage was repeated four times, so there were 28 units. The results of  phosphate solubilizing and nitrogen fixer bacteria could produce more rice grain 0.32 ton.ha<sup>-1</sup> than NPK 100% (recommended dose). Biological soil fertility improved with the increased population of bacteria, but there was no improved soil chemical fertility of soil.

scholarly journals Kenaf (Hibiscus cannabinus L.) as a remedy to oxisol contaminated with different mercury (Hg2+) concentrations

2021 ◽  
pp. 780-786
Author(s):  
Letícia Fernanda Lavezzo ◽  
Denise de Lima Dias Delarica ◽  
Anne Hélène Fostier ◽  
Everlon Cid Rigobelo ◽  
Roberta Souto Carlos ◽  
...  
Keyword(s):  
Transfer Factor ◽  
Absorption Spectrometry ◽  
Soil Samples ◽  
Hibiscus Cannabinus ◽  
The Other ◽  
Controlled Environment ◽  
Randomized Design ◽  
Total Hg ◽  
Completely Randomized Design ◽  
Soil Hg

We evaluated Hibiscus cannabinus (kenaf) to remedy oxisol contaminated with Hg2 + potential. The study was conducted in a controlled environment in pots with soil contaminated with HgCl2 solution, in a completely randomized design with 4 treatments: control (without Hg2+) and treatments with 5, 24 and 36 mg Hg2+ kg-1 of soil and 5 replicates / treatment. The quantification of total Hg in plant and soil samples was performed by atomic absorption spectrometry. Kenaf grown in contaminated pots did not show visual symptoms of toxicity. Plant height did not differ among treatments, but the dry shoot phytomass was 21.65% higher in control than the average of treatments with Hg. Treatment with 24 mg kg-1 showed dry root phytomass greater than control and the others. In general, oxisol was responsible for retaining greater amount of Hg than plants. Hg accumulated in greater proportion in roots than in shoots. In pots that received 36 mg kg-1, plants accumulated average of 2.57 mg kg-1 of Hg / pot, differing from the other treatments and the Hg transfer factor (TF) in plants was also calculated as the ratio of the concentration in shoots and in roots. The values were as follows: 3.11 for T1, 1.26 for T2, 0.05 for T3 and 0.02 for T4. Treatments showed no difference between T3 and T4 and TF decreased with increasing Hg dose. It could be concluded that Hg was more adsorbed by oxisol than by plants. Plants showed resistance to different soil Hg concentrations and can be considered as potential Hg2+ stabilizer

scholarly journals Korelasi Antara Daya Serap Gracilaria sp. Terhadap Konsentrasi Logam Berat Cu di Media Pemeliharaan

2021 ◽  
Vol 10 (3) ◽  
pp. 363-368
Author(s):  
Regina Amanda ◽  
Ria Azizah Tri Nuraini ◽  
Endang Supriyantini
Keyword(s):  
Water Pollution ◽  
Heavy Metals ◽  
Heavy Metal ◽  
Weight Gain ◽  
Metal Content ◽  
Absorption Capacity ◽  
Control Treatment ◽  
Living Things ◽  
Randomized Design ◽  
Completely Randomized Design

Pencemaran air terhadap lingkungan dapat menyebabkan dampak membahayakan yang dapat dirasakan oleh makhluk hidup. Salah satu logam berat yang sering mencemari air adalah logam berat tembaga (Cu). Tujuan penelitian ini untuk mengetahui kemampuan daya serap rumput laut Gracilaria sp. terhadap logam berat Cu dan pertumbuhannya. Penelitian ini menggunakan metode eksperimental laboratoris dengan Rancangan Acak Lengkap (RAL) dengan menggunakan 4 perlakuan penambahan konsentrasi Cu, yaitu kontrol (0,54 ppm, sesuai dengan konsentrasi Cu pada air laut), 3 ppm, 6 ppm, 9 ppm dengan 3 kali pengulangan. Pengukuran kandungan logam Cu terhadap Gracilaria sp. dengan menggunakan Atomic Absorpsion Spektofotometri (AAS). Hasil penelitian menunjukkan bahwa penyerapan logam berat Cu tertinggi terdapat pada konsentrasi 9 ppm, yaitu sebesar 3,63 ppm dan terendah pada kontrol yaitu sebesar 0,27 ppm.. Pertumbuhan Gracilaria sp. tertinggi terdapat pada perlakuan kontrol dengan pertambahan berat sebesar 209,2 gram dan  terendah pada konsentrasi 9 ppm, yaitu sebesar 162,9 gram. Semakin tinggi konsentrasi Cu yang diberikan maka semakin rendah pertumbuhan Gracilaria sp. Water pollution to the environment can cause dangerous impacts that can be felt by living things. One of the heavy metals that often pollutes water is the heavy metal copper (Cu). The purpose of this study was to determine the absorption capacity of Gracilaria sp. to the heavy metal Cu and its growth. This study used a laboratory experimental method with a completely randomized design (RAL) using 4 additional treatments of Cu concentration, namely control (0.54 ppm, according to the Cu concentration in seawater), 3 ppm, 6 ppm, 9 ppm with 3 repetitions. Measurement of Cu metal content against Gracilaria sp. by using Atomic Absorption Spectophotometry (AAS). The results showed that the highest absorption of heavy metal Cu was at a concentration of 9 ppm, which was 3.63 ppm and the lowest was 0,27 ppm in control.. The Growth of Gracilaria sp. The highest was found in the control treatment with a weight gain of 209.2 grams and the lowest was at a concentration of 9 ppm, which was 162.9 grams. The higher the concentration of Cu given, the lower the growth of Gracilaria sp. 

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