The influence of the corpus luteum on ovarian follicular dynamics during estrous synchronization in goats

2004 ◽  
Vol 84 (3-4) ◽  
pp. 369-375 ◽  
Author(s):  
A Lassala ◽  
J Hernández-Cerón ◽  
R Rodrı́guez-Maltos ◽  
C.G Gutierrez
Keyword(s):  
Corpus Luteum ◽  
Estrous Synchronization ◽  
Follicular Dynamics

Studies on Ovarian Follicular Dynamics and Steroid Profiles in Sahiwal Cattle

2021 ◽  
Author(s):  
Vinny Dodiyar ◽  
Parkash Singh Brar ◽  
Narinder Singh ◽  
Mrigank Honparkhe
Keyword(s):  
Corpus Luteum ◽  
Estrous Cycle ◽  
Maximum Diameter ◽  
Endocrine Regulation ◽  
Dominant Follicle ◽  
Estrous Cycles ◽  
Significant Difference ◽  
Sahiwal Cattle ◽  
Sahiwal Cows ◽  
Follicular Dynamics

Background: Understanding of ovarian follicular dynamics and endocrine regulation is essential to design and use interventions to optimize reproductive efficiency. Bos indicus and Bos taurus cows have some differences in their follicular dynamics and ovarian steroidal hormones. The present study was planned to understand follicular dynamics vis-a-vis ovarian steroids profiles in Sahiwal cattle. Methods: The study was conducted in normal cyclic, pluriparous, non-lactating Sahiwal cows (n=7). Trans-rectal ultrasonography was performed to record the location, size, number of follicles and size of corpus luteum (CL) from the beginning, till the end of experiment. The dataset was used to characterize the follicular wave emergence, growth, regression, time of selection and ovulation of the dominant follicle in each cow. Result: Three Sahiwal cows showed two wave and four cows showed three wave estrous cycles with mean interovulatory length of 20.33±0.33 and 22.50±0.28 days, respectively. No significant difference was recorded in the maximum diameter of corpus luteum, P4 and E2 plasma levels between 2-wave and 3-wave estrous cycles. The peak progesterone values of 6.00±0.91 and 6.2±1.2 ng/ml and peak estradiol values of 15.83±0.60 and 14.31±0.44 ng/ml were recorded in 2-wave and 3-wave estrous cycle, respectively. The results showed that Sahiwal cows had 2-wave and 3-wave estrous cycle and the 3-wave estrous cycle had comparatively longer inter-ovulatory period and smaller maximum diameter of second wave dominant follicle than 2-wave estrous cycle.

174 EVALUATION OF THE 9-DAY PROTOCOL FOR ESTROUS SYNCHRONIZATION IN SANTA INÊS EWES

2011 ◽  
Vol 23 (1) ◽  
pp. 189
Author(s):  
P. Viau ◽  
M. B. Paes de Barros ◽  
L. M. K. Dias ◽  
S. S. Nicolau ◽  
C. T. Marino ◽  
...  
Keyword(s):  
Solid Phase ◽  
Device Removal ◽  
Intact Male ◽  
Estrous Synchronization ◽  
Follicular Wave ◽  
Vaginal Progesterone ◽  
Device Placement ◽  
Santa Inês ◽  
Veterinary Hospital ◽  
Follicular Dynamics

The objective of the present study was to characterise follicular dynamics in Santa Ines (SI) during a 9-day protocol for oestrous synchronization and to evaluate the efficiency of the vaginal progesterone-releasing device Primer-PR® (Tecnopec, Brazil). Cyclic females were used (n = 10) at the ruminant sector of the veterinary hospital of the FMVZ–USP, in March 2009. The animals had body scores between 2.5 and 3 and were of proven fertility. Ultrasound examinations were performed using an ALOKA SSD-500 Scanner (Berger, Brazil) with a linear 5.0-MHz transducer, attached to a handle to allow safe intrarectal manipulation. Examinations were performed daily from 3 days (D-3) before the day of device placement (D0) until the day of device removal (D9), and twice daily from device removal until ovulation. All ewes received 0.03 mg of prostaglandin (D-cloprostenol, Prolise®, Tecnopec, Brazil) on D9. Ovulation was assessed by the disappearance of the growing larger follicle or follicles present in the previous examinations. Oestrous detection was performed using an intact male 3 times a day (at 10:00 a.m., 6:00 p.m., and 12:00 p.m.) from D9 until the last acceptant ewe, and blood samples were taken by jugular puncture for progesterone (P4) measurement by radioimmunoassay on solid phase (COAT-A-COUNT, Siemens, USA) from D-3 until the day of ovulation. Data were analysed by Shapiro–Wilk (PROC UNIVARIATE) using the SAS program (SAS Institute Inc., Cary, NC, USA, 2001) and shown as mean ± standard deviation. Plasma P4 concentrations between D0 and D9 were 6.56 ± 2.32 ng mL–1, peaking between D3 and D5 (8.07 ± 2.31 ng mL–1). Oestrous behaviour was shown 45.6 ± 12.71 h after Primer-PR® removal. The first and the last ewe that showed oestrous behaviour was at 30 h and 66 h after Primer-PR® removal, respectively, and the majority of ewes (50%) at 42 h. Oestrous lasted 26.40 ± 9.47 h, and the majority of ewes (70%) showed oestrous behaviour during 24 h. Ovulation occurred 73 ± 14.38 h after Primer–PR® removal and 1.3 ± 0.48 ovulations per animal were observed. From all growing presumptively dominant follicles observed, 92.3% of them ovulated. When double ovulations occurred (n = 3), the interval between first and second ovulation was 16 ± 6.93 h. Emergence of the ovulatory follicular wave occurred at 8.5 days + 16 h after Primer–PR® insertion. The follicles observed to continue growing had a diameter of 3.48 ± 0.28 mm when they were first detected and reached 5.63 ± 0.66 mm, with a growth rate of 0.73 ± 0.43 mm per day. A standard follicle wave within the 9-day protocol was not possible to determine, and the follicles receded in up to 4 days. In conclusion, placement of a Primer-PR® device for 9 days resulted in synchronous oestrus and ovulation in Santa Inês ewes. FAPESP, CAPES, Tecnopec.

Locational relationship between corpus luteum and ovulatory follicle on ovaries alters follicular dynamics and progesterone concentrations of Thai indigenous beef cows exhibiting two follicular waves

2019 ◽  
Vol 59 (12) ◽  
pp. 2161
Author(s):  
P. Yama ◽  
T. Moonmanee ◽  
M. Osathanunkul ◽  
J. Jitjumnong ◽  
W. Karaphuak
Keyword(s):  
Growth Rate ◽  
Corpus Luteum ◽  
Beef Cows ◽  
Follicular Waves ◽  
Ultrasound Monitoring ◽  
Ovulatory Follicle ◽  
The Mean ◽  
The Impact ◽  
The Relationship ◽  
Follicular Dynamics

The present study aims to determine the impact of differences in the locational relationship between the previous corpus luteum (CL) and the further ovulatory follicle (OF) on follicular dynamics and progesterone (P4) concentrations in Thai indigenous beef cows (White Lamphun) exhibiting two follicular waves. Twenty-one cows, exhibiting the two-wave follicular pattern, were studied through interovulatory intervals (IOI), and classified according to the relationship between the previous CL and the further OF on the cattle model ovaries. Classifications were outlined as either an ipsilateral (same ovary) relationship (n = 12), or a contralateral (opposite ovaries) relationship (n = 9). Ultrasound monitoring, which evaluated the follicular diameter, and collection of blood for determining the P4 concentration were performed each day throughout the IOI. The IOI was longer (P < 0.05) in the contralateral cows than in the ipsilateral cows (19.7 ± 0.33 days vs 18.5 ± 0.29 days). Cows with an ipsilateral relationship were found to have further OFs with greater (P < 0.05) diameters than were cows with a contralateral relationship (13.9 ± 0.31 mm vs 12.1 ± 0.21 mm). The mean growth rate of the further OF was greater (P = 0.05) in the ipsilateral cows than in the contralateral cows (1.1 ± 0.11 mm/day vs 0.8 ± 0.04 mm/day). On Day 17 of the IOI, the ipsilateral cows demonstrated their lowest concentration of P4 (P < 0.05). On Day 18 of the IOI, the concentrations of P4 tended to be lower (P = 0.09) in the ipsilateral cows than in the contralateral cows (0.6 ± 0.04 ng/mL vs 1.1 ± 0.12 ng/mL). The interval from the luteinisation until the end of the luteolysis was longer (P < 0.05) in the contralateral group than in the ipsilateral group (18.5 ± 0.50 days vs 16.7 ± 0.33 days). Thus, we conclude that in Thai indigenous beef cows, the growth rate and diameter of the further OF during luteolysis increases more in the ipsilateral relationship than in the contralateral relationship.

118 A retrospective analysis of follicular dynamics, LH, estradiol-17β, and progesterone in prostaglandin F2α-induced estrus of Beetal goats

2019 ◽  
Vol 31 (1) ◽  
pp. 185
Author(s):  
A. Murtaza ◽  
M. I. R. Khan ◽  
W. Ahmad ◽  
T. Sohail ◽  
I. Mohsin ◽  
...  
Keyword(s):  
Single Dose ◽  
Corpus Luteum ◽  
Luteal Phase ◽  
Linear Models ◽  
Variable Interval ◽  
Prostaglandin F2α ◽  
Follicle Diameter ◽  
Coefficient Corrélation ◽  
Student’S T ◽  
Follicular Dynamics

The objective of the study was to reveal the factors associated with variable interval to ovulation following a single dose of prostaglandin F2α (PGF2) in Beetal goats. In experiment 1, the extent of ovulation time was demonstrated after synchronizing Beetal goats (n=38) by using a single injection of PGF2 based on corpus luteum detection by B-mode transrectal ultrasonography. Ovulations were detected every 6h from PGF2 until ovulation. To explore the changes in follicular dynamics, endocrine profiles and behavioural events due to the variation in the interval to ovulation, another set of goats (n=13; experiment 2) were synchronized by a single dose of PGF2 given at random days of the luteal phase. Follicular dynamics were monitored every 12h from PGF2 until ovulation and oestrus was detected by apronized bucks every 6h from PGF2 treatment. Blood sampling (3mL) for oestradiol-17β and progesterone was done every 12h from PGF2 until ovulation and every 2h for LH assay by radioimmunoassay. Data were analysed by Student’s t-test, general linear models, and Pearson coefficient correlation (SPSS, version 20.0, SPSS Inc., Chicago, IL, USA). Results revealed that ovulation time varied from 60 to 96h after PGF2, and 57% of ovulations occurred by 72h after PGF2 (experiment 1). Based on this observation, goats (n=13) in second experiment were retrospectively divided into early and late ovulators (i.e. 72 and 84h following PGF2, respectively). Onset of oestrus, peak oestradiol-17β concentration, and LH surge after PGF2 were first observed in early than late ovulators (P<0.05). The goats having greater follicle diameter and smaller corpus luteum diameter at the time of PGF2 ovulated earlier than late ovulators (5.4±0.2v. 4.3±0.2mm and 10±0.6v. 11.8±0.3mm, respectively; P<0.05). Likewise, plasma progesterone concentration tended to be lower (P=0.087) in early than late ovulators. However, interval from onset of oestrus to peak LH and ovulation, duration of standing oestrus, interval from LH peak to ovulation, diameter and growth rate of preovulatory follicles, and number of ovulations were similar (P>0.05) between early and late ovulators. In conclusion, the size of dominant follicle and corpus luteum at the time of PGF2 determines the interval to ovulation following a single dose of PGF2 during the luteal phase.

scholarly journals The effect of GnRH or oestradiol injected at pro-oestrus on luteal function and follicular dynamics of the subsequent oestrous cycle in non-lactating cycling Holstein cows

2006 ◽  
Vol 73 (1) ◽  
Author(s):  
B.V.E. Segwagwe ◽  
K.L. MacMillan ◽  
P.D. Mansell
Keyword(s):  
Corpus Luteum ◽  
Optimum Concentration ◽  
New Wave ◽  
Luteal Function ◽  
Future Studies ◽  
Induction Of Ovulation ◽  
Follicular Wave ◽  
Oestradiol Benzoate ◽  
Lower Fertility ◽  
Follicular Dynamics

Oestrous synchronization involves synchronization of ovarian follicular turnover, new wave emergence, and finally induction of ovulation. The final step can be synchronized by the parenteral administration of either GnRH or oestradiol benzoate. This study investigated corpus luteum and follicular emergence after ovulation had been induced by the administration of either GnRH or oestradiol benzoate. The injection of oestradiol benzoate may have delayed the emergence of the first follicular wave subsequent to the induced ovulation; administration of oestradiol benzoate or GnRH lowered the progesterone rise so that the maximum dioestrous concentration of progesterone on Day 9 was lower when cows were treated during pro-oestrus compared to the spontaneously ovulating controls. One implication of findings from the present study is that induction of ovulation with either oestradiol benzoate or GnRH, administered 24 or 36 h after withdrawal of the CIDR device, respectively, may lower fertility. Future studies must identify the timing of administration relative to the time of CIDR device withdrawal and the optimum concentration of oestradiol benzoate or GnRH that would not have untoward effects on the development of the corpus lutea, particularly within the first week of dioestrus.

scholarly journals Follicle and corpus luteum size and vascularity as predictors of fertility at the time of artificial insemination and embryo transfer in beef cattle

2015 ◽  
Vol 35 (5) ◽  
pp. 470-476 ◽  
Author(s):  
Fábio L.V. Pinaffi ◽  
Ériko S. Santos ◽  
Maurício G. da Silva ◽  
Milton Maturana Filho ◽  
Ed H. Madureira ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Corpus Luteum ◽  
Embryo Transfer ◽  
Artificial Insemination ◽  
Prediction Methods ◽  
Estrous Synchronization ◽  
Hormone Profile ◽  
Lower Fertility ◽  
Follicle Size ◽  
First Time

Abstract:Two ultrasound based fertility prediction methods were tested prior to embryo transfer (ET) and artificial insemination (AI) in cattle. Female bovines were submitted to estrous synchronization prior to ET and AI. Animals were scanned immediately before ET and AI procedure to target follicle and corpus luteum (CL) size and vascularity. In addition, inseminated animals were also scanned eleven days after insemination to target CL size and vascularity. All data was compared with fertility by using gestational diagnosis 35 days after ovulation. Prior to ET, CL vascularity showed a positive correlation with fertility, and no pregnancy occurred in animals with less than 40% of CL vascularity. Prior to AI and also eleven days after AI, no relationship with fertility was seen in all parameters analyzed (follicle and CL size and vascularity), and contrary, cows with CL vascularity greater than 70% exhibit lower fertility. In inseminated animals, follicle size and vascularity was positive related with CL size and vascularity, as shown by the presence of greater CL size and vascularity originated from follicle with also greater size and vascularity. This is the first time that ultrasound based fertility prediction methods were tested prior to ET and AI and showed an application in ET, but not in AI programs. Further studies are needed including hormone profile evaluation to improve conclusion.

Follicular dynamics, corpus luteum growth and regression in lactating dairy cattle

1991 ◽  
Vol 71 (1) ◽  
pp. 61-68 ◽  
Author(s):  
C. Taylor ◽  
R. Rajamahendran
Keyword(s):  
Dairy Cows ◽  
Corpus Luteum ◽  
Estrous Cycle ◽  
Follicular Growth ◽  
Dominant Follicle ◽  
Lactating Dairy Cows ◽  
Ovulatory Follicle ◽  
Second Wave ◽  
Lactating Dairy Cattle ◽  
Follicular Dynamics

Ultrasound imaging and progesterone (P4) profiles were used to characterize follicular and corpus luteum (CL) growth and regression in 10 cycling dairy cows. Thirteen of sixteen cycles were characterized by two waves of follicular growth, the second wave giving rise to the ovulatory follicle. The remaining three cycles had three waves. There was no difference between two- and three-wave cycles with regard to the day of emergence of the first or second dominant follicle (day 2.5 ± 0.9 vs. day 2.4 ± 1.2 and day 12.4 ± 1.6 vs. day 12.0 ± 2.0, respectively) or the onset of regression of the first dominant follicle (day 12.5 ± 1.5 vs. day 12.3 ± 1.5). In two wave cycles, estrous cycle length was shorter (20.8 ± 1.7 d vs. 29.7 ± 4.9 d), the first day of decline in P4 (day 16.2 ± 1.5 vs. day 23.7 ± 5.5) and the onset of CL regression (day 17.1 ± 1.3 vs. day 24.3 ± 5.5) was earlier. It is concluded that two waves of follicular growth in an estrous cycle is the norm in lactating dairy cows. The presence of an additional wave is due to a prolonged luteal phase. Key words: Follicle, corpus luteum, estrous cycle, dairy cow

Sign in / Sign up

Export Citation Format

Share Document