Effects of orexin A on PTGS2, PTGES, CBR1 and PGFS mRNA transcript abundances and prostaglandin E2 and F2α concentrations in culture medium of pig uterine explants collected during early gestation and the oestrous cycle

2021 ◽  
pp. 106910
Author(s):  
Kinga Orzechowska ◽  
Marta Kiezun ◽  
Edyta Rytelewska ◽  
Marlena Gudelska ◽  
Katarzyna Kisielewska ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Culture Medium ◽  
Oestrous Cycle ◽  
Mrna Transcript ◽  
Orexin A ◽  
Early Gestation

scholarly journals Effects of stage of oestrous cycle and progesterone supplementation during culture on maturation of canine oocytes in vitro

Reproduction ◽  
2003 ◽  
pp. 501-508 ◽  
Author(s):  
LA Willingham-Rocky ◽  
K Hinrichs ◽  
ME Westhusin ◽  
DC Kraemer
Keyword(s):  
Selection Criteria ◽  
In Vitro Maturation ◽  
Culture Medium ◽  
Oestrous Cycle ◽  
In Vitro Experiments ◽  
Meiotic Arrest ◽  
Treatment Groups ◽  
Key Factor ◽  
Progesterone Supplementation

The aim of this study was to evaluate the effect of progesterone supplementation and stage of oestrous cycle on in vitro maturation (IVM) of canine oocytes. Oocytes were cultured in medium supplemented with 0, 2000, 4000 or 8000 ng progesterone ml(-1) (Expt 1; n=274 oocytes) or 0, 20, 200 or 2000 ng progesterone ml(-1) (Expt 2; n=789 oocytes). In Expt 3, oocytes (n=1202) were cultured in a bi-phasic system of meiotic arrest followed by IVM, both in the presence of 0, 20, 200 or 2000 ng progesterone ml(-1). Rates of meiotic resumption for Expt 1 ranged from 40.0% to 58.5%; there were no significant differences among groups. In Expt 2, rate of meiotic resumption was significantly lower in the 2000 ng progesterone ml(-1) treatment (35.5%) compared with the 200 ng progesterone ml(-1) treatment (54.0%; P<0.05). There were no significant differences in rates of maturation to metaphase II among treatments in Expt 1 (1.8-8.6%) or Expt 2 (8.4-14.7%); however, oocytes collected from ovaries of bitches in oestrus and dioestrus had higher rates of maturation to metaphase II than did oocytes from bitches at pro-oestrus or anoestrus (P<0.01). In Expt 3, no differences were observed in rates of maturation among treatment groups. Rates of maturation to metaphase II of oocytes from bitches in dioestrus were significantly higher than those from bitches in pro-oestrus (P<0.01). These results indicate that supplementation of culture medium with progesterone either during maturation or during meiotic arrest before maturation does not increase the rate of IVM of canine oocytes. However, stage of oestrous cycle is a key factor in the selection criteria for meiotically competent canine oocytes for use in in vitro experiments.

Effect of the conceptus on uterine prostaglandin-F2α and prostaglandin-E2 release and synthesis during the periimplantation period in the pig

2009 ◽  
Vol 21 (5) ◽  
pp. 709 ◽  
Author(s):  
Marta Wasielak ◽  
Katarzyna Kamińska ◽  
Marek Bogacki
Keyword(s):  
Protein Expression ◽  
Prostaglandin E2 ◽  
Prostaglandin F2α ◽  
Systemic Effect ◽  
Local Effect ◽  
Uterine Horn ◽  
Oestrous Cycle ◽  
Prostaglandin Synthase ◽  
Pge2 Concentration ◽  
Pg Release

The present study was conducted to evaluate the effect of the conceptus on uterine prostaglandin-F2α (PGF2α) and prostaglandin-E2 (PGE2) release and the expression of prostaglandin synthase enzymes during the periimplantation period in the pig. A surgically generated model with conceptuses developing in only one of the uterine horns was created. The highest concentration of PGF2α and PGE2 was found in the gravid uterine horn, compared with the non-gravid horn and the intact horn of cyclic gilts. Endometrial concentration of both PGs in pregnant gilts was elevated regardless of the conceptus in the uterine horn, whereas only myometrial PGE2 concentration increased during pregnancy. Expression of prostaglandin-E2 synthase (mPGES-1) mRNA in the endometrium was upregulated during the oestrous cycle, while protein expression presented a similar pattern to that of PGE2 concentration in the uterine flushings. Prostaglandin-F2α synthase (PGFS) mRNA and protein expression in the endometrium did not differ between pregnancy and oestrous cycle but PGFS mRNA in the myometrium increased during pregnancy both in the gravid and the non-gravid uterine horns. We suggest a local effect of the conceptus on PG release pathways but also a more systemic effect within the whole uterus with regard to PG synthesis and accumulation in uterine tissues.

The effect of orexin A on CYP17A1 and CYP19A3 expression and on oestradiol, oestrone and testosterone secretion in the porcine uterus during early pregnancy and the oestrous cycle

2017 ◽  
Vol 90 ◽  
pp. 129-140 ◽  
Author(s):  
Marta Kiezun ◽  
Nina Smolinska ◽  
Kamil Dobrzyn ◽  
Karol Szeszko ◽  
Edyta Rytelewska ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Oestrous Cycle ◽  
Orexin A ◽  
Testosterone Secretion

scholarly journals Effect of LH on prostaglandin F2α and prostaglandin E2 secretion by cultured porcine endometrial cells

Reproduction ◽  
2005 ◽  
Vol 130 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Agnieszka Blitek ◽  
Adam J Ziecik
Keyword(s):  
Epithelial Cells ◽  
Prostaglandin E2 ◽  
Stromal Cells ◽  
Prostaglandin F2α ◽  
Cell Types ◽  
Oestrous Cycle ◽  
Time Dependent ◽  
Dependent Effect ◽  
Endometrial Cells ◽  
Time Dependent Effect

LH appears to be a potent stimulator of the release of endometrial prostaglandins (PGs) in the pig. The aim of the present studies was to examine the effect of LH on PGF2αand PGE2secretion by cultured porcine endometrial cells on days 10–12 and 14–16 of the oestrous cycle and to compare its action with oxytocin. A time-dependent effect of LH (10 ng/ml) on PGF2αrelease from luminal epithelial and stromal cells on days 10–12 was observed (experiment 1). The highest increase in PGF2αsecretion in response to LH was detected in stromal cells after 6 h of incubation (P< 0.001). Epithelial cells responded to LH after a longer exposure time (P< 0.01). A concentration-dependent effect of LH (0.1–100 ng/ml) on PGF2αrelease from stromal cells was examined after 6 h and from epithelial cells after 12 h (experiment 2). Effective concentrations of LH were 10 and 100 ng/ml. LH (10 ng/ml) and oxytocin (100 nmol/l) affected PGF2αand PGE2secretion from endometrial cells on days 10–12 and 14–16 of the oestrous cycle (experiment 3). LH stimulated PGF2αsecretion from both cell types and its action was more potent on days 10–12. LH induced PGE2release, especially in epithelial cells on days 14–16. A stimulatory effect of oxytocin on PGF2αwas confirmed in stromal cells, but this hormone was also shown to enhance PGE2output. These results indicated that LH, like oxytocin, a very effective stimulator of PGF2αrelease, could play an important role in the induction of luteolysis.

Cyclic and diurnal alterations in the response of luteinizing hormone and prolactin to prostaglandin E2 during the rat oestrous cycle

1984 ◽  
Vol 106 (1) ◽  
pp. 30-37 ◽  
Author(s):  
Lise Wogensen ◽  
Jørgen Warberg
Keyword(s):  
Luteinizing Hormone ◽  
Prostaglandin E2 ◽  
Lateral Ventricle ◽  
Mature Female ◽  
Oestrous Cycle ◽  
Female Rats ◽  
Male Rats ◽  
Prostaglandin E ◽  
The Brain ◽  
Time Of Administration

Abstract. Two μg of prostaglandin E2 (PGE2) was infused into a lateral ventricle of the brain of female rats at 09.00 or 13.00 h on the different days of the oestrous cycle and the effect on luteinizing hormone (LH) and prolactin (Prl) release was determined. At 09.00 h PGE2 caused a pronounced release of LH in pro-oestrous, oestrous and metoestrous rats whereas the LH response in dioestrous rats was moderate. The secretion of Prl was only stimulated in rats from the pro-oestrous phase. When infused at 13.00 h PGE2 had a marked stimulatory effect on the release of LH in all groups of rats. The response was almost the same in oestrous, metoestrous and dioestrous rats but pro-oestrous rats a 2-fold higher LH response was observed. On each day of the oestrous cycle it was found that the LH-releasing activity of PGE2 was greater at 13.00 h than at 09.00 h. Thus, the overall greatest responsiveness of LH to PGE2 was noted at 13.00 h on pro-oestrus i.e. at a time which was prior to the onset of the spontaneous LH surge. At 13.00 h – as at 09.00 h – PGE2 was only capable of stimulating Prl release in pro-oestrous rats. Resembling the LH response it was found that PGE2-induced Prl release was greater at 13.00 h than at 09.00 h. In adult male rats the stimulatory effect of PGE2 on LH and Prl release was independent of the time of administration. It is concluded that the neuroendocrine elements of the hypothalamo-pituitary unit in mature female rats exhibit cyclic as well as diurnal alterations in the responsiveness to PGE2.

Termination of early gestation with 9-deoxo-16,16-dimethyl-9-methylene prostaglandin E2

Contraception ◽  
1982 ◽  
Vol 26 (3) ◽  
pp. 261-277 ◽  
Author(s):  
Paul F. Brenner ◽  
Richard P. Marrs ◽  
Subir Roy ◽  
Daniel R. Mishell
Keyword(s):  
Prostaglandin E2 ◽  
Early Gestation

Interactions between large and small luteal cells collected during the mid- or late-luteal stages of the bovine oestrous cycle

1995 ◽  
Vol 7 (1) ◽  
pp. 35 ◽  
Author(s):  
Vecchio RP Del ◽  
JK Thibodeaux ◽  
R Saatman ◽  
W Hansel
Keyword(s):  
Arachidonic Acid ◽  
Treatment Group ◽  
Luteinizing Hormone ◽  
Culture Medium ◽  
Oestrous Cycle ◽  
Control Group ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Treatment Groups ◽  
Progesterone Production

The effects of contact between large and small bovine luteal cells together with those of luteinizing hormone (LH) or arachidonic acid (AA) on progesterone production during the oestrous cycle were investigated. Corpora lutea were collected during the mid-luteal stage (Days 10-12; n = 4) and late-luteal stage (Days 17-18; n = 4) of the oestrous cycle. Large and small luteal cells were dispersed and separated and then incubated together or separately. Mid-luteal stage cells were treated with LH (0 or 5 ng) whereas late-luteal stage cells were treated with LH (0 or 5 ng) or AA (0 or 10 microM). Culture medium was collected and replaced 1, 3 and 6 h after starting treatments. Progesterone production decreased (P < 0.0001) with increased incubation time irrespective of cell arrangement, the stage of the oestrous cycle or treatment. During the 18 h before treatment, cells in the contact arrangement produced more progesterone (P < 0.003) than cells without contact in both mid- and late-luteal stages of the oestrous cycle; progesterone production within cell arrangements between prospective treatment groups was similar. After initiating treatments, mid-luteal stage cells in the control group without contact produced more progesterone (P < 0.01) than cells with contact. Mid-luteal stage cells treated with LH produced more (P < 0.0001) than control cells; progesterone production between cell arrangements within the LH treatment group was similar. In the late-luteal stage cells, both LH and AA increased (P < 0.01) progesterone production by comparison with control cells; LH and AA treatment groups produced similar results.(ABSTRACT TRUNCATED AT 250 WORDS)

121 RELATIONSHIP BETWEEN PROSTAGLANDIN E2 METABOLITE, UTERINE ARTERIAL VASODILATORY CHANGES, AND PROGESTERONE DURING THE OESTROUS CYCLE AND EARLY PREGNANCY IN BEEF COWS

2013 ◽  
Vol 25 (1) ◽  
pp. 207
Author(s):  
H. L. Sánchez-Rodríguez ◽  
J. M. Feugang ◽  
R. C. Youngblood ◽  
R. C. Vann ◽  
S. T. Willard ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Early Pregnancy ◽  
Vascular Function ◽  
Reproductive Tract ◽  
Sharp Decrease ◽  
Female Reproductive Tract ◽  
Oestrous Cycle ◽  
Potential Candidate ◽  
Vascular Effects ◽  
Uterine Arteries

Vasodilation of the female reproductive vasculature is essential to supply the increasing demands associated with reproductive processes; including maintenance of a healthy functional luteal structure, and the establishment and continuance of pregnancy. Due to its vasodilatory effects, prostaglandin E2 has been identified as a potential candidate sustaining such vascular effects. Here, the relationship between systemic concentrations of prostaglandin E2 metabolite (PGEM) and progesterone (P4), and uterine arterial vasodilatory changes were evaluated during the oestrous cycle and early pregnancy. Synchronised Angus cows (n = 19) were artificially inseminated (AI) and examined for pregnancy 45 d post-AI. Jugular blood samples were collected on Days 0, 3, 6, 10, 16, 20, 25, and 32 (Day 0 = AI) for serum PGEM and P4 evaluation. Vasodilatory changes of the uterine arteries were also characterised using the B-mode ultrasound luminal circumference (UAC). Retrospective comparisons were carried out between pregnant (n = 10) and nonpregnant (n = 9) animals. The GLM Procedure of SAS (SAS Institute Inc., Cary, NC, USA) was used to evaluate the effects of pregnancy status and sampling day over PGEM, UAC, and P4. Pearson correlations between PGEM, UAC, and P4 were obtained by the CORR Procedure of SAS. All variables were measured in triplicate and thresholds for significance and tendency were fixed at P ≤ 0.05 and 0.06 to 0.10, respectively. From Day 0 to Day 20, the overall PGEM concentration tended to be higher in nonpregnant cows compared to pregnant ones (P = 0.08; 90.97 ± 4.38 v. 78.28 ± 3.01 pg mL–1, respectively), while a shift in favor of pregnant cows was observed at Day 32 (P = 0.29; 89.88 ± 14.23 v. 106.3 ± 7.17 pg mL–1, respectively). Both uterine arteries showed similar UAC (P ≥ 0.05); therefore, they were combined in one average value per animal each sampling day. There was an overall increase of the UAC over time, from 10.49 ± 0.01 mm at Day 0 to 12.17 ± 0.02 mm at Day 20 (P ≤ 0.0001), but no significant differences were found between pregnant and nonpregnant cows during this period (P ≥ 0.05). An overall sharp increase in P4 concentrations was observed from Day 3 to Day 16 (0.60 ± 0.12 to 9.66 ± 0.45 ng mL–1, P ≤ 0.0001); while from Day 16 to Day 32, pregnant cows maintained steady and higher P4 concentrations than nonpregnant ones (9.87 ± 0.31 v. 4.40 ± 1.09 ng mL–1, respectively; P = 0.002). As expected, nonpregnant cows showed a sharp decrease in P4 concentrations from Day 16 to Day 25, followed by a second increase at Day 32 (9.27 ± 0.20, 0.85 ± 0.33, and 6.04 ± 1.67 ng mL–1, respectively; P = 0.0007). The UAC and PGEM concentrations were positively correlated in pregnant (r = 0.48; P = 0.002), but not in nonpregnant animals (r = 0.29; P = 0.21). Similarly, UAC and P4 concentrations were also positively associated in pregnant (r = 0.47; P = 0.002), but not in nonpregnant cows (r = 0.01; P = 0.96). Our findings support the role of PGE2 as a potential mediator of the vascular function in the female reproductive tract and, subsequently, as an essential factor to ensure adequate P4 production able to sustain early pregnancy in the bovine. USDA-ARS Grant#58-6402-3-0120.

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