Interactions between large and small luteal cells collected during the mid- or late-luteal stages of the bovine oestrous cycle

1995 ◽  
Vol 7 (1) ◽  
pp. 35 ◽  
Author(s):  
Vecchio RP Del ◽  
JK Thibodeaux ◽  
R Saatman ◽  
W Hansel
Keyword(s):  
Arachidonic Acid ◽  
Treatment Group ◽  
Luteinizing Hormone ◽  
Culture Medium ◽  
Oestrous Cycle ◽  
Control Group ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Treatment Groups ◽  
Progesterone Production

The effects of contact between large and small bovine luteal cells together with those of luteinizing hormone (LH) or arachidonic acid (AA) on progesterone production during the oestrous cycle were investigated. Corpora lutea were collected during the mid-luteal stage (Days 10-12; n = 4) and late-luteal stage (Days 17-18; n = 4) of the oestrous cycle. Large and small luteal cells were dispersed and separated and then incubated together or separately. Mid-luteal stage cells were treated with LH (0 or 5 ng) whereas late-luteal stage cells were treated with LH (0 or 5 ng) or AA (0 or 10 microM). Culture medium was collected and replaced 1, 3 and 6 h after starting treatments. Progesterone production decreased (P < 0.0001) with increased incubation time irrespective of cell arrangement, the stage of the oestrous cycle or treatment. During the 18 h before treatment, cells in the contact arrangement produced more progesterone (P < 0.003) than cells without contact in both mid- and late-luteal stages of the oestrous cycle; progesterone production within cell arrangements between prospective treatment groups was similar. After initiating treatments, mid-luteal stage cells in the control group without contact produced more progesterone (P < 0.01) than cells with contact. Mid-luteal stage cells treated with LH produced more (P < 0.0001) than control cells; progesterone production between cell arrangements within the LH treatment group was similar. In the late-luteal stage cells, both LH and AA increased (P < 0.01) progesterone production by comparison with control cells; LH and AA treatment groups produced similar results.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Effect of Ferula communis L. on reproductive parameters in Awassi ewes

2019 ◽  
Vol 70 (3) ◽  
pp. 1625
Author(s):  
A.G. ONAL
Keyword(s):  
Reproductive Hormones ◽  
Control Group ◽  
Corpora Lutea ◽  
Reproductive Parameters ◽  
Treatment Groups ◽  
Progesterone Production ◽  
Awassi Sheep ◽  
To Receive ◽  
Follicular Activity

Nutrient composition of the diet affects follicular activity, embryonic development and reproductive hormones in ruminants. The objective of the present study was to investigate the effects of Ferula communis L. on some reproductive parameters of Awassi sheep. The experiment was carried out on 29 (15-16 months old) Awassi ewes. All ewes were allocated to receive either a control (14% CP and 11.7 MJ ME/kg, n=9) or a diet supplemented with 5% (75g, n=10) or 10% (150g, n=10) powdered F. communis root, respectively for 21 days. Oestrus was synchronized using intravaginal sponges, while oestrus behaviour was observed 24, 36 and 48h after the sponge removal. Blood samples were collected for the assessment of oestradiol and LH. At the end of the 21-day period, animals were slaughtered and ovarian structures were recorded. Corpora lutea tissues were cultured in vitro, and progesterone production was measured. The results indicate that the treatment of animals with 5% of F. communis root increased the percentage of animals in heat (80%, 60% and 10% for 5C, 10C, and the control group, respectively). Furthermore, the number of small follicles (1-3 mm) in treated groups was significantly higher than those of the control group. Moreover, the number of large follicles (>4 mm) in the control group was higher than those of the treatment groups. Plasma concentration of oestrogen and LH peak were similar in the control and treatment groups. Progesterone production by luteal cells cultured in vitro was higher for both treatment groups compared to the control. Herewith, supplementation of the diet of Awassi Sheep with F. communis root during the breeding season may enhance ovulation rate and luteal activity. 

scholarly journals Effects of imidacloprid and thiamethoxam on the development and reproduction of the soybean aphid Aphis glycines

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0250311
Author(s):  
Aonan Zhang ◽  
Lin Zhu ◽  
Zhenghao Shi ◽  
Tianying Liu ◽  
Lanlan Han ◽  
...  
Keyword(s):  
Treatment Group ◽  
Reproductive Rate ◽  
Soybean Aphid ◽  
Aphis Glycines ◽  
Intrinsic Rate Of Increase ◽  
Control Group ◽  
Net Reproductive Rate ◽  
Treatment Groups ◽  
Rate Of Increase ◽  
The Mean

The soybean aphid Aphis glycines Matsumura (Hemiptera: Aphididae) is a primary pest of soybeans and poses a serious threat to soybean production. Our studies were conducted to understand the effects of different concentrations of insecticides (imidacloprid and thiamethoxam) on A. glycines and provided critical information for its effective management. Here, we found that the mean generation time and adult and total pre-nymphiposition periods of the LC50 imidacloprid- and thiamethoxam-treatment groups were significantly longer than those of the control group, although the adult pre-nymphiposition period in LC30 imidacloprid and thiamethoxam treatment groups was significantly shorter than that of the control group. Additionally, the mean fecundity per female adult, net reproductive rate, intrinsic rate of increase, and finite rate of increase of the LC30 imidacloprid-treatment group were significantly lower than those of the control group and higher than those of the LC50 imidacloprid-treatment group (P < 0.05). Moreover, both insecticides exerted stress effects on A. glycines, and specimens treated with the two insecticides at the LC50 showed a significant decrease in their growth rates relative to those treated with the insecticides at LC30. These results provide a reference for exploring the effects of imidacloprid and thiamethoxam on A. glycines population dynamics in the field and offer insight to agricultural producers on the potential of low-lethal concentrations of insecticides to stimulate insect reproduction during insecticide application.

scholarly journals THE TRAINING OF GRATEFULNESS IN IMPROVING LIFE MEANING OF CONVICTS AT CLASS 2 LPKA PEKANBARU

2020 ◽  
Vol 6 (1) ◽  
pp. 42-47
Author(s):  
Vivik Shofiah ◽  
Khairunnas Rajab
Keyword(s):  
Treatment Group ◽  
Meaning Of Life ◽  
T Test ◽  
Average Score ◽  
Control Group ◽  
Life Meaning ◽  
Treatment Groups ◽  
Know How ◽  
Post Test ◽  
Test Control

Religious values need are very imporant for convicts at LPKA Class two Pekanbaru. Religion is an obvious influenced source in issuing the meaning of life to convicts. The research objective is to know how far the influence of gratefulness training in improving life meaning of convicts at LPKA class two Pekanbaru is.The researcher used experiment approaching pre and post-test control group designwith 23 control groupsand 23 treatment groups,anddata analysis independent sample t-test was obtained score  t= 6,894 (p<0,01). Average score from treatment group are higher  (23,057) than control group average (15,6). It shows that life meaning of convicts who get gratefulness training are higher than convicts who don’t join the training. With thus, it can be concluded that gratefulness training very influence significantly to the life meaning of convicts at LPKA class 2 Pekanbaru.

Cat dissection vs. sculpting human structures in clay: an analysis of two approaches to undergraduate human anatomy laboratory education

2005 ◽  
Vol 29 (1) ◽  
pp. 27-34 ◽  
Author(s):  
John R. Waters ◽  
Peggy Van Meter ◽  
William Perrotti ◽  
Salvatore Drogo ◽  
Richard J. Cyr
Keyword(s):  
Treatment Group ◽  
Student Performance ◽  
Student Attitudes ◽  
Experimental Treatment ◽  
Human Anatomy ◽  
Control Group ◽  
Isolation And Identification ◽  
Treatment Groups ◽  
Laboratory Education ◽  
Anatomy Laboratory

Many human anatomy courses are taught using cat dissection. Alternatives are available, but information regarding learning outcomes is incomplete. In 2003, ∼120 undergraduates enrolled in a human anatomy course were assigned to one of two treatment groups. In the control group, students performed cat dissections (emphasizing isolation and identification) of the muscular, digestive, and cardiovascular systems. In the experimental treatment group, students built clay sculptures of each human body system. Student learning was evaluated by using both low- and high-difficulty questions. On pre- and postexperiment control exams, there were no significant differences in student performance. On exams after a cat dissection vs. a human-clay sculpting experience, the students in the human-clay sculpting treatment group scored significantly higher than their classmates in the cat dissection group on both the low- and high-difficulty questions. Student attitudes toward dissection and taking future human anatomy courses were also measured. There were no differences in student attitudes at the beginning of the experiment; afterward, students exposed to a cat dissection experience viewed dissection more favorably than students in the human-clay sculpting treatment group. There were no treatment effects on student willingness to take future human anatomy courses. The experimental design makes it difficult to conclude precisely why students assigned to the human-clay sculpting experience performed better on exams, but as each method was performed in this particular human anatomy course, our data indicate that human-clay sculpting may be a viable alternative to cat dissection in an anatomy course in which the students focus on human anatomy.

scholarly journals Sublethal Effects of Imidacloprid on the Population Development of Western Flower Thrips Frankliniella occidentalis (Thysanoptera: Thripidae)

Insects ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 3 ◽  
Author(s):  
Yu Cao ◽  
Hong Yang ◽  
Jun Li ◽  
Chun Wang ◽  
Can Li ◽  
...  
Keyword(s):  
Treatment Group ◽  
Frankliniella Occidentalis ◽  
Sublethal Effects ◽  
Western Flower Thrips ◽  
Effective Control ◽  
Control Group ◽  
Population Development ◽  
Treatment Groups ◽  
Flower Thrips ◽  
Low Concentrations

The Western flower thrips (WFT, Frankliniella occidentalis) is a global polyphagous pest that is often dependent on chemical control. Imidacloprid has been a commonly used chemical insecticide for effective control of WFT. Low concentrations of insecticides can have sublethal effects on individual insects. However, no more information is known about the effects of exposure at low concentrations of imidacloprid on WFT. Here, we evaluated the effects of imidacloprid at sublethal concentrations on WFT population growth parameters. We first exposed the parental generation to LC10 (56.8 mg/L) and LC25 (79.2 mg/L) concentrations of imidacloprid. We then quantified various parameters related to the development, survival, and fecundity of the F1 generation also exposed to these same concentrations. The development time of the treatment groups exposed to imidacloprid was significantly shorter than the control group, and the net reproductive rate (R0) was significantly higher for treatment groups than for the control group. For both variables, there was no significant difference between LC10 and LC25 exposure. The generational survival rate was significantly higher for the control group, followed by the LC10 treatment group and then the LC25 treatment group. However, the opposite was true for fecundity and intrinsic rate of increase (rm) of WFT. In summary, exposure to low concentrations of imidacloprid was positive for the population development of WFT, which may contribute to the development of insecticide resistance and cause resurgence in WFT populations.

scholarly journals Anti-Inflammatory Effect of Musa acuminata Stem

2020 ◽  
Vol 14 (02) ◽  
pp. 294-298
Author(s):  
Maharani Laillyza Apriasari ◽  
Selviana Rizky Pramitha ◽  
Dewi Puspitasari ◽  
Diah Savitri Ernawati
Keyword(s):  
Treatment Group ◽  
Musa Acuminata ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Tnf Α ◽  
Significant Difference ◽  
Anti Inflammatory ◽  
Mucosal Wound ◽  
Inflammatory Effect

Abstract Objective This study was designed to assess the anti-inflammatory effect of Musa acuminata through the expression of tumor necrosis factor-α (TNF-α) and nuclear factor kappa β (NF-κB) after 3 days of application of Musa acuminata stem extract (MASE) gel on oral mucosal wound. Materials and Methods An experimental study with post-test only control group design was conducted. Twenty male Rattus norvegicus (Wistar) were injured on their left buccal mucosa and treated three times a day with MASE gel of varying concentrations: 0% (as control), MASE 25%, MASE 37.5%, and MASE 50%. On day 3, a biopsy was performed on each mucosal wound for later immunohistochemical analysis for the expressions of TNF-α and NF-κB. Results The highest expression of TNF-α was observed in the control group (13.20 ± 1.79), while the lowest was in the treatment group using 50% MASE (6.40 ± 1.14). Meanwhile the comparison between treatment groups did not highlight any significant difference (p > 0.05). The highest expression of NF-κB was observed in the control group (13.20 ± 1.30), whereas the lowest was in the treatment group using MASE 50% (6.40 ± 1.14). NF-κB was significantly lower in the treatment group using MASE 50% when compared with other treatment groups (p < 0.05). Conclusion Application of MASE on mucosal wound reduces the expression of TNF-α and NF-κB at all concentrations. The anti-inflammatory effect of MASE 50% was the strongest one.

Effects on Gingivitis of Two Different 0.4% SnF2 Gels

1988 ◽  
Vol 67 (2) ◽  
pp. 503-507 ◽  
Author(s):  
R.L. Boyd ◽  
P.J. Leggott ◽  
P.B. Robertson
Keyword(s):  
Treatment Group ◽  
High Availability ◽  
Plaque Index ◽  
Control Group ◽  
Bleeding Tendency ◽  
Gingival Index ◽  
Periodontal Health ◽  
Pocket Depth ◽  
Treatment Groups ◽  
The One

For nine months we monitored the periodontal health of 81 adolescents undergoing orthodontic treatment with fixed appliances, to determine whether daily use of a brush-on 0.4% SnF 2 gel would be better than toothbrushing alone in maintaining periodontal health in these patients, and whether a gel supplying a high percentage of available Sn2+ ion would be more beneficial than a gel supplying a low percentage of available Sn2+ ion. The subjects were matched for age and sex and placed into a control group, which used toothbrushing alone, and two treatment groups, which used toothbrushing supplemented with daily use of a SnF2 gel. One treatment group used a gel with 98% available Sn2+ ion twice daily for the entire nine months. The other treatment group used a gel with less than 2% available Sn2+ once a day for six months, then twice a day for the remaining three months of the study. Clinical assessments (Plaque Index, Gingival Index, Bleeding Tendency, pocket depth, and coronal staining) were made before appliances were placed and at one, three, six, and nine months after appliances were placed. Results indicated that the group using the high-availability Sn 2+ gel twice daily had significantly lower Gingival Index and Bleeding Tendency scores at the one-, three-, six-, and nine-month examinations than did the control group. The group using the low-availability Sn2+ gel showed no significant differences in these assessments from the control group. Neither treatment group showed significant differences from the control group in Plaque Index or pocket depth. In the group using the high-availability Sn2+ gel, one subject developed mild coronal staining, and two developed moderate staining.

Oxytocin may play a role in the control of the human corpus luteum

1982 ◽  
Vol 95 (1) ◽  
pp. 65-70 ◽  
Author(s):  
G. J. S. Tan ◽  
R. Tweedale ◽  
J. S. G. Biggs
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Inhibitory Action ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Human Corpus Luteum

The effects of oxytocin on dispersed luteal cells from human corpora lutea of the menstrual cycle were studied. Oxytocin at a concentration of 4 mi.u./ml produced a slight increase in basal progesterone production. However, higher oxytocin concentrations (400 and 800 mi.u./ml) markedly inhibited both basal and human chorionic gonadotrophin-induced progesterone production. These data provide evidence for an effect of oxytocin on the human corpus luteum. In view of the inhibitory action of oxytocin, increased secretion of this hormone may be important in the demise of the corpus luteum at the end of the menstrual cycle.

CHANGES IN THE DENSITY AND PROGESTERONE CONTENT OF LUTEAL TISSUE IN THE EGYPTIAN BUFFALO DURING THE OESTROUS CYCLE

1967 ◽  
Vol 39 (2) ◽  
pp. 163-171 ◽  
Author(s):  
A. S. EL-SHEIKH ◽  
FRANÇOIS B. SAKLA ◽  
SAFAA O. AMIN
Keyword(s):  
Corpus Luteum ◽  
Cell Volume ◽  
Distribution System ◽  
Oestrous Cycle ◽  
Luteal Cell ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Functional Changes ◽  
Parallel Increase ◽  
Luteal Tissue

SUMMARY The histological and functional changes of 31 corpora lutea of Egyptian buffaloes during the various phases of the oestrous cycle were studied. The volumes of the corpora lutea were calculated, the volume per cell, the cell volume and the volume of the intercellular spaces were estimated from transverse serial sections stained with haematoxylin and eosin, Mallory's triple stain or van Gieson's stain. The nuclear volumes were also determined and the cytoplasmic volume was calculated. The progesterone content was estimated using column absorption chromatography and a counter-current distribution system. It was concluded that the luteal cells increase both in volume and in number due to mitosis. The luteal cells decrease in volume after the 15th day after ovulation, the cells lose their distinct outlines in the regressive stage and disappear completely in the corpus albicans. There was a parallel increase in luteal cell volume and progesterone content until the 15th post-ovulatory day followed by a decrease in the regressive phase and disappearance of the hormone in the corpus albicans. A highly significant correlation (r = +0·875) was found between the progesterone content and the cytoplasmic volume. Progesterone concentration/g. luteal tissue increased from the corpus haemorrhagicum to the mature corpus luteum, decreased in the regressive corpus luteum and completely disappeared in the corpus albicans.

PROGESTERONE AND LUTEINIZING HORMONE CONCENTRATION IN SERUM OF PREGNANT GILTS ON DIFFERENT LEVELS OF FEED CONSUMPTION

1980 ◽  
Vol 60 (4) ◽  
pp. 877-884 ◽  
Author(s):  
G. W. DYCK ◽  
W. M. PALMER ◽  
S. SIMARAKS
Keyword(s):  
Luteinizing Hormone ◽  
Feed Consumption ◽  
Blood Collection ◽  
Corpora Lutea ◽  
Serum Progesterone ◽  
Treatment Groups ◽  
Fetal Survival ◽  
Serum Lh ◽  
Luteinizing Hormone Concentration ◽  
Collection Period

Blood serum progesterone (P) and luteinizing hormone (LH) were determined in 31 Lacombe gilts at a 3- to 4-day interval from 10 to 56 days of pregnancy. They were fed 1.50 (L), 2.25 (M) or 3.00 (H) kg of a 16% protein diet/day and had average daily gains of 0.16 ± 0.02 (SE), 0.36 ± 0.02 and 0.60 ± 0.02 kg/day, respectively. All gilts were slaughtered between 59 and 61 days of pregnancy. The treatments had no effect on the number of corpora lutea (13.9 ± 0.4 CL), number of fetuses (10.8 ± 0.6), fetal survival (78.3 ± 3.7%) or mean fetal weight (122 ± 3 g). The gilts on the H level of feeding had heavier (P < 0.05) ovaries (18.4 ± 0.8 g) than those of the L (15.6 ± 0.7 g) or M (15.6 ± 0.8 g) treatment groups. Serum P concentration declined during the blood collection period (b = −0.12, −0.06 and −0.09 for the L, M and H groups, respectively). Serum P concentrations also were different (P < 0.01) for each treatment group (L, 16.7 ± 0.3; M, 13.8 ± 0.03; and H, 11.8 ± 0.3 ng/mL). The serum LH concentration was greater (P < 0.05) for the M gilts (0.63 ± 0.05 ng/mL) than for the L (0.37 ± 0.04 ng/mL) or H (0.47 ± 0.05 ng/mL) gilts. Significant correlations were observed among the number of fetuses, number of CL, ovarian weight and P for the L gilts, and the number of fetuses and CL for the M gilts. These results suggest that growth rate is a major contributor to differences in blood P and LH concentrations during pregnancy and that the animal adjusts its P and LH production to its specific requirements for the maintenance of pregnancy.

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