Dual-function injectable fibrin gel incorporated with sulfated chitosan nanoparticles for rhBMP-2-induced bone regeneration

2022 ◽  
Vol 26 ◽  
pp. 101347
Author(s):  
Xinye Chen ◽  
Shuang Wang ◽  
Xiaohui Zhang ◽  
Yuanman Yu ◽  
Jing Wang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Chitosan Nanoparticles ◽  
Dual Function ◽  
Fibrin Gel

scholarly journals Photocuring Hyaluronic Acid/Silk Fibroin Hydrogel Containing Curcumin Loaded CHITOSAN Nanoparticles for the Treatment of MG-63 Cells and ME3T3-E1 Cells

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2302
Author(s):  
Qingwen Yu ◽  
Zhiyuan Meng ◽  
Yichao Liu ◽  
Zehao Li ◽  
Xing Sun ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Bone Regeneration ◽  
Water Uptake ◽  
Silk Fibroin ◽  
Bone Repair ◽  
Ph Value ◽  
Chitosan Nanoparticles ◽  
Vitro Assay ◽  
Long Term Treatment

After an osteosarcoma excision, recurrence and bone defects are significant challenges for clinicians. In this study, the curcumin (Cur) loaded chitosan (CS) nanoparticles (CCNP) encapsulated silk fibroin (SF)/hyaluronic acid esterified by methacrylate (HAMA) (CCNPs-SF/HAMA) hydrogel for the osteosarcoma therapy and bone regeneration was developed by photocuring and ethanol treatment. The micro or nanofibers networks were observed in the CCNPs-SF/HAMA hydrogel. The FTIR results demonstrated that alcohol vapor treatment caused an increase in β-sheets of SF, resulting in the high compression stress and Young’s modulus of CCNPs-SF/HAMA hydrogel. According to the water uptake analysis, SF caused a slight decrease in water uptake of CCNPs-SF/HAMA hydrogel while CCNPs could enhance the water uptake of it. The swelling kinetic results showed that both the CCNPs and the SF increased the swelling ratio of CCNPs-SF/HAMA hydrogel. The accumulative release profile of CCNPs-SF/HAMA hydrogel showed that the release of Cur from CCNPs-SF/HAMA hydrogel was accelerated when pH value was decreased from 7.4 to 5.5. Besides, compared with CCNPs, the CCNPs-SF/HAMA hydrogel had a more sustainable drug release, which was beneficial for the long-term treatment of osteosarcoma. In vitro assay results indicated that CCNPs-SF/HAMA hydrogel with equivalent Cur concentration of 150 μg/mL possessed both the effect of anti-cancer and promoting the proliferation of osteoblasts. These results suggest that CCNPs-SF/HAMA hydrogel with superior physical properties and the bifunctional osteosarcoma therapy and bone repair may be an excellent candidate for local cancer therapy and bone regeneration.

scholarly journals Electrospun PCL scaffold modified with chitosan nanoparticles for enhanced bone regeneration

2021 ◽  
Author(s):  
Ameneh Seddighian ◽  
Fariba Ganji ◽  
Mohamadreza Baghaban-Eslaminejad ◽  
Fatemeh Bagheri
Keyword(s):  
Bone Regeneration ◽  
Chitosan Nanoparticles

Vascularization and bone regeneration in a critical sized defect using 2-N,6-O-sulfated chitosan nanoparticles incorporating BMP-2

Biomaterials ◽  
2014 ◽  
Vol 35 (2) ◽  
pp. 684-698 ◽  
Author(s):  
Lingyan Cao ◽  
Jing Wang ◽  
Juan Hou ◽  
Wanli Xing ◽  
Changsheng Liu
Keyword(s):  
Bone Regeneration ◽  
Chitosan Nanoparticles ◽  
Critical Sized Defect

scholarly journals Synthesis of Nano Chitosan as Carrier Material of Cinnamon’s Active Component

2018 ◽  
Vol 21 (2) ◽  
pp. 92-97 ◽  
Author(s):  
Ngadiwiyana Ngadiwiyana ◽  
Enny Fachriyah ◽  
Purbowatiningrum Ria Sarjono ◽  
Nor Basid Adiwibawa Prasetya ◽  
Ismiyarto Ismiyarto ◽  
...  
Keyword(s):  
Particle Size ◽  
Electron Microscope ◽  
Chitosan Nanoparticles ◽  
Dual Function ◽  
Ftir Analysis ◽  
Natural Polymer ◽  
Mild Conditions ◽  
Natural Extracts ◽  
Deacetylation Degree ◽  
Transmission Electron

Development and innovation to improve the efficacy of active ingredients of a plant can be done by using nanoparticle encapsulation of chitosan, which has dual function of protecting natural extracts degradation and delivering natural extracts to the target site. Chitosan is a natural polymer that is nontoxic, mucoadhesive, biodegradable, and biocompatible. This polymer also has a low level of immunogenicity and can be prepared into nanoparticles in mild conditions that make it suitable for natural extracts delivery systems. This paper reported synthesis of chitosan nanoparticles for cinnamon’s natural extract delivery. Chitosan synthesis was carried out by chitin deacetylation isolated from shrimp shells. Chitosan characterization was done by measuring deacetylation degree by FTIR. Chitosan nanoparticles were prepared by ionic gelation method using tripolyphosphate as crosslinker. Morphology and particle size of nano chitosan were characterized using a scanning electron microscope (SEM) and a transmission electron microscope (TEM). The result found that the yield of deproteinated chitin was 62.60%. Further process of demineralization resulted a yield of 52.60%, then depigmentation with a yield of 75.56%, and deacetylation with a yield of 79.02%. FTIR analysis showed that deacetylation degree of chitin into chitosan was found of 87.78%. Characterization by SEM found that nano chitosan has a particle size of 87 nm. While TEM images showed that the nano chitosan has a uniform shape and a lower physical aggregation.

scholarly journals Pre-vascularization in fibrin Gel/PLGA microsphere scaffolds designed for bone regeneration

2018 ◽  
Vol 10 (8) ◽  
pp. 827-839 ◽  
Author(s):  
Jun Liu ◽  
Gang Chen ◽  
Hai Xu ◽  
Ke Hu ◽  
Jianfei Sun ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Plga Microsphere ◽  
Fibrin Gel

Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles

Biomaterials ◽  
2014 ◽  
Vol 35 (9) ◽  
pp. 2730-2742 ◽  
Author(s):  
Lingyan Cao ◽  
Jerome A. Werkmeister ◽  
Jing Wang ◽  
Veronica Glattauer ◽  
Keith M. McLean ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Chitosan Nanoparticles

scholarly journals In Vitro and In Vivo Evaluation of Commercially Available Fibrin Gel as a Carrier of Alendronate for Bone Tissue Engineering

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Beom Su Kim ◽  
Feride Shkembi ◽  
Jun Lee
Keyword(s):  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Release Kinetics ◽  
Human Mesenchymal Stem Cells ◽  
Calvarial Defect ◽  
Defect Model ◽  
Fibrin Gel ◽  
In Vivo Evaluation

Alendronate (ALN) is a bisphosphonate drug that is widely used for the treatment of osteoporosis. Furthermore, local delivery of ALN has the potential to improve the bone regeneration. This study was designed to investigate an ALN-containing fibrin (fibrin/ALN) gel and evaluate the effect of this gel on both in vitro cellular behavior using human mesenchymal stem cells (hMSCs) and in vivo bone regenerative capacity. Fibrin hydrogels were fabricated using various ALN concentrations (10−7–10−4 M) with fibrin glue and the morphology, mechanical properties, and ALN release kinetics were characterized. Proliferation and osteogenic differentiation of and cytotoxicity in fibrin/ALN gel-embedded hMSCs were examined. In vivo bone formation was evaluated using a rabbit calvarial defect model. The fabricated fibrin/ALN gel was transparent with Young’s modulus of ~13 kPa, and these properties were not affected by ALN concentration. The in vitro studies showed sustained release of ALN from the fibrin gel and revealed that hMSCs cultured in fibrin/ALN gel showed significantly increased proliferation and osteogenic differentiation. In addition, microcomputed tomography and histological analysis revealed that the newly formed bone was significantly enhanced by implantation of fibrin/ALN gel in a calvarial defect model. These results suggest that fibrin/ALN has the potential to improve bone regeneration.

scholarly journals BMP6-Engineered MSCs Induce Vertebral Bone Repair in a Pig Model: A Pilot Study

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Gadi Pelled ◽  
Dmitriy Sheyn ◽  
Wafa Tawackoli ◽  
Deuk Soo Jun ◽  
Youngdo Koh ◽  
...  
Keyword(s):  
Pilot Study ◽  
Bone Regeneration ◽  
Ex Vivo ◽  
Pig Model ◽  
Invasive Technique ◽  
Vertebral Compression Fractures ◽  
Control Group ◽  
Large Animal ◽  
Fibrin Gel

Osteoporotic patients, incapacitated due to vertebral compression fractures (VCF), suffer grave financial and clinical burden. Current clinical treatments focus on symptoms’ management but do not combat the issue at the source. In this pilot study, allogeneic, porcine mesenchymal stem cells, overexpressing the BMP6 gene (MSC-BMP6), were suspended in fibrin gel and implanted into a vertebral defect to investigate their effect on bone regeneration in a clinically relevant, large animal pig model. To check the effect of the BMP6-modified cells on bone regeneration, a fibrin gel only construct was used for comparison. Bone healing was evaluated in vivo at 6 and 12 weeks and ex vivo at 6 months. In vivo CT showed bone regeneration within 6 weeks of implantation in the MSC-BMP6 group while only minor bone formation was seen in the defect site of the control group. After 6 months, ex vivo analysis demonstrated enhanced bone regeneration in the BMP6-MSC group, as compared to control. This preclinical study presents an innovative, potentially minimally invasive, technique that can be used to induce bone regeneration using allogeneic gene modified MSCs and therefore revolutionize current treatment of challenging conditions, such as osteoporosis-related VCFs.

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