Biohardening with Plant Growth Promoting Rhizosphere and Endophytic bacteria induces systemic resistance against Banana bunchy top virus

Background: Symbiotic associations between legumes and Rhizobia are ancient and fundamental. However, the plant growth-promoting endophytes other than Rhizobia are not yet fully explored for pulses productivity. The present study was aimed to isolate efficient endophytic bacteria from pulses, assess their diversity, screen their plant growth-promoting activities and to test their potential as bio inoculants for pulses.Methods: We have isolated several endophytic bacteria from pulse crops more specifically from blackgram (Vigna mungo) and greengram (Vigna radiata). After careful screening, 15 promising endophytic isolates were selected for this study. The identification of endophytic bacterial isolates was performed by 16S rRNA gene sequencing. The isolates were tested for their potential for the plant growth-promoting traits such as nitrogen fixation, phosphate solubilization, indole-3-acetic acid production, siderophore secretion and antifungal activity. Pot culture experiments were conducted with the screened potential endophytic cultures.Result: The 16S rRNA gene sequencing revealed that species of Enterobacter, Bacillus, Pantoea, Pseudomonas, Acromobacter, Ocrobacterium were found as endophytes in blackgram and greengram. The in vitro screening identified Bacillus pumilus (BG-E6), Pseudomonas fluorescens (BG-E5) and Bacillus licheniformis (BG-E3) from blackgram and Pseudomonas chlororaphis (GG-E2) and Bacillus thuringiensis (GG-E7) from greengram as potential plant growth-promoting endophytes. These strains showed antagonism against plant pathogenic fungi. Upon inoculation of these endophytic PGPR strains, the blackgram and greengram growth and yield got increased. Among the strains, BG-E6 recorded 14.7% increased yield in blackgram and GG-E2 accounted for a 19.5% yield increase in greengram compared to respective uninoculated control. The experimental results showed that there was a host specificity found among the endophytic bacterial cultures with pulses. The cross inoculation of endophytic strains did not perform well to enhance the growth and yield of their alternate hosts.

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Isolasi dan Karakterisasi Bakteri Endofit Pelarut Fosfat dan Penghasil Hidrogen Cyanide (HCN) dari Tanaman Bawang Merah (Allium cepa L).

Bioma Berkala Ilmiah Biologi ◽

10.14710/bioma.20.1.9-16 ◽

2018 ◽

Vol 20 (1) ◽

pp. 9

Author(s):

Ryan Hilda Wandita ◽

Sri Pujiyanto ◽

Agung Suprihadi ◽

Ratih Dewi Hastuti

Keyword(s):

Plant Growth ◽

Traditional Medicine ◽

Allium Cepa ◽

Endophytic Bacteria ◽

Economic Value ◽

Average Diameter ◽

Plant Growth Promoting Bacteria ◽

Allium Cepa L ◽

Plant Growth Promoting ◽

Growth Promoting

Onions (Allium cepa L.) is one of the leading horticultural commodities in Indonesia and is often used as seasoning and traditional medicine. Onion has a high economic value and fluctuating prices so that domestic onion production needs to be improved, one of them with a presence of endophytic bacteria that act as plant growth promoting agent or Plant Growth Promoting Bacteria (PGPB). Endophytic bacteria isolated from the root, leaves, and bulbs. In this research has been tested endophytic bacteria of onion plants from Garut regency which has PGPB factors such as able to dissolve phosphate, and produce HCN. The results obtained 251 isolates of endophytic bacteria. Based on the characterization results, the superior isolates capable of dissolving phosphate with an average diameter of 0.45 cm is isolate II.B.1D.3, and 11 isolates capable of producing high HCN. These isolates can be used as PGPB agents so that they can be useful in increasing plant growth and onion production and biocontrol in suppressing pathogens. Keywords: PGPB, endophyte, onion, phosphate, HCN

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Molecular characterization and identification of plant growth promoting endophytic bacteria isolated from the root nodules of pea (Pisum sativum L.)

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-013-1488-9 ◽

2013 ◽

Vol 30 (2) ◽

pp. 719-725 ◽

Cited By ~ 37

Author(s):

Mohsin Tariq ◽

Sohail Hameed ◽

Tahira Yasmeen ◽

Mehwish Zahid ◽

Marriam Zafar

Keyword(s):

Pisum Sativum ◽

Plant Growth ◽

Molecular Characterization ◽

Endophytic Bacteria ◽

Root Nodules ◽

Pisum Sativum L ◽

Plant Growth Promoting ◽

Growth Promoting

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Plant growth-promoting rhizobacteria induced resistance in Jatropha curcas through phenyl propanoid metabolism against Rhizoctoniabataticola

Journal of Applied and Natural Science ◽

10.31018/jans.v9i1.1159 ◽

2017 ◽

Vol 9 (1) ◽

pp. 121-128

Author(s):

S. Kumar ◽

M. Singh ◽

Sushil Sharma

Keyword(s):

Plant Growth ◽

Pseudomonas Fluorescens ◽

Jatropha Curcas ◽

Root Rot ◽

Plant Growth Promoting Rhizobacteria ◽

Systemic Resistance ◽

Total Phenols ◽

Phenyl Propanoid ◽

Plant Growth Promoting ◽

Growth Promoting

The root rot disease in Jatropha curcas L. caused by Rhizoctonia. bataticola (Taub.) Butler has been recorded in causing 10-12 per cent mortality of 20-30 days old seedlings of Jatropha curcasin southern Haryana. The incidence of this disease has also been observed from other parts of Haryana too. Induction of systemic resistance in host plants through microbes and their bioactive metabolites are attaining popularity in modern agricultural practices. Studies on the plant growth-promoting rhizobacteria induced resistance in Jatropha curcas through phenyl propanoid metabolism against Rhizoctoniabataticola were undertaken at Chaudhary Charan Singh, Haryana Agricultural University, Regional Research Station, Bawal. Three plant growth-promoting rhizobacteria (PGPRs) viz., Pseudomonas maltophila, Pseudomonas fluorescens and Bacillus subtilis were evaluated for their potential to induce systemic resistance in Jatropha against root rot. The maximum increase of 97 per cent in total phenols, 120 per cent in peroxidase, 123 per cent in polyphenol oxidase, 101 per cent in phenylalanine ammonia lyase and 298 per cent in tyrosine ammonia lyase was detected in plants raised with Pseudomonas fluorescens+ Rhizoctoniaba-taticola inoculation in Jatropha curcas at 10 days post inoculation against control except total phenols where it was maximum (99%) at 30 DPI. There was slight or sharp decline in these parameters with age irrespective of inoculations. The pathogen challenged plants showed lower levels of total phenols and enzymes. The observations revealed that seed bacterization with Pseudomonas fluorescens results in accumulation of phenolics and battery of enzymes in response to pathogen infection and thereby induce resistance systemically.

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Biocontrol potential; antifungal activity and plant growth promoting activities of endophytic bacteria from Raphanus sativus

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v12i2.4646 ◽

2021 ◽

Vol 12 (2) ◽

pp. 1143-1150

Author(s):

Lavanya J ◽

Chanthosh S ◽

Reshma Shrii ◽

Viknesh V ◽

Deepika S ◽

...

Keyword(s):

16S Rrna ◽

Plant Growth ◽

Raphanus Sativus ◽

Endophytic Bacteria ◽

Effective Strain ◽

Pathogenic Fungi ◽

Plant Diseases ◽

Plant Pathogenic Fungi ◽

Plant Growth Promoting ◽

Growth Promoting

The study was aimed to find an alternate approach for chemicals used in agriculture to avoid microbial infections. Fungal pathogens cause different types of plant diseases and affect a majority of edible crops by destroying the tissues of the plant in a direct or indirect mechanism. So, an alternative approach led to the development of biocontrol agents using endophytic bacteria. A total of 8 endophytic bacteria were isolated from the root, stem, and leaves of radish (Raphanus sativus). The antagonistic activity of these bacteria against the 2 isolated plant pathogenic fungi was determined in vitro. Two out of eight bacteria showed more than 50% inhibitory activity against one fungus, were further characterized using the 16s rRNA sequencing method. On the basis of the phylogenetic tree of the 16s rRNA method, the endophytic bacterial samples were identified as Tonsilliphilus suis and Exiguobacterium aurantiacum against plant pathogenic Aspergillus flavus isolated from Raphanus sativus, which makes them highly suitable as an alternative for chemical fertilizers to provide resistance to plant pathogenic fungi. The cell wall degrading activities such as protease activity, amylase activity, and plant growth-promoting properties such as Hydrogen cyanide (HCN), Indole acetic acid (IAA), ammonia production of these endophytic bacteria were evaluated. The results show that T. suis is the most effective strain for radish growth development.

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