Different via to apply the Gamaxine® commercial biopromoter to Nile tilapia evaluating the immune system responses to Streptococcus agalactiae Ib

Aquaculture ◽  
2019 ◽  
Vol 503 ◽  
pp. 254-266 ◽  
Author(s):  
Marco Shizuo Owatari ◽  
Gabriel Fernandes Alves Jesus ◽  
Lucas Cardoso ◽  
Tamiris Henrique Ferreira ◽  
José Victor Safadi Ferrarezi ◽  
...  
Keyword(s):  
Immune System ◽  
Streptococcus Agalactiae ◽  
Nile Tilapia

scholarly journals The used of chopped banana Musa paradisiaca stem for stimulating immune responses and streptococcosis resistance of Nile tilapia Oreochromis niloticus

2018 ◽  
Vol 17 (2) ◽  
pp. 147
Author(s):  
Lilis Nurjanah ◽  
Sri Nuryati ◽  
Alimuddin Alimuddin ◽  
Kukuh Nirmala
Keyword(s):  
Immune System ◽  
Streptococcus Agalactiae ◽  
Respiratory Burst ◽  
Nile Tilapia ◽  
Lysozyme Activity ◽  
Time Interval ◽  
Active Compounds ◽  
Percent Survival ◽  
Banana Stem ◽  
Replacement Time

<p align="center"><strong>ABSTRACT</strong></p><p align="center"><strong> </strong></p><p>Streptococcosis caused by <em>Streptococcus agalactiae</em> bacteria, is a type of disease that often found in Nile tilapia farming. This study was performed to determine the effectiveness of the concentration and frequency of giving chopped banana stem as the immunostimulant to stimulate the non‒specific immune system of tilapia against <em>S. agalactiae</em> infection. This study used factorial completely randomized design, consisted of two factors: concentration and changing frequency (replacement time interval) of banana stem on Nile tilapia rearing media with 11 treatment and each treatment was given three replications. The concentration of 5 g/L, 10 g/L and 15 g/L and the three days, seven days and no replacement time interval were used in this study. The immersion treatment with the chopped banana stem was done for 14 days, then the challenge test with <em>S. agalactiae</em> was on the 15th day for 14 days observation. The result showed that chopped banana stem contained active compounds of alkaloids, namely: 17.63% flavonoids, 0.02% tannin and 0.24% saponins. This active compounds could increase the non-specific immune system including respiratory burst<em>, </em>lysozyme activity, phagocyte activity, erythrocyte, leucocyte and haemoglobin. Treatment with a concentration of 5 g/L and no replacement of the chopped banana stem was the best treatment with 75% relative percent survival, highest than other treatments. In addition, it also has highest phagocytes (36.3%), respiratory burst<em> </em>(0.58 at O.D. 630), and lysozyme activity (72.7 unit/mL) after four days challenged with <em>S. agalactiae.</em></p><p> </p><p>Keywords: Banana stem, immunostimulant, Nile tilapia, non-specific immune system, <em>Streptococcus agalatiae</em></p><p><em> </em></p><p> </p><p> </p><p align="center"><strong>ABSTRAK</strong></p><p> </p><p>Penyakit <em>Streptococcosis</em> yang disebabkan oleh bakteri <em>Streptococcus agalactiae</em> merupakan jenis penyakit  yang sering ditemukan pada budidaya ikan nilaPenelitian ini bertujuan untuk menentukan konsentrasi dan frekuensi pergantian cacahan batang pisang sebagai imunostimulan yang optimum sehingga dapat meningkatkan daya tahan tubuh ikan nila terhadap serangan penyakit <em>streptococcosis</em> yang disebabkan oleh bakteri <em>S. agalactiae</em>. Penelitian ini menggunakan rancangan acak lengkap faktorial. Perlakuan terdiri dari dua faktor yaitu konsentrasi dan frekuensi pergantian cacahan batang pisang pada media pemeliharaan ikan dengan 11 perlakuan dan 3 ulangan. Konsentras batang pisang yang digunakan yaitu 5 g/L, 10 g/L dan 15 g/L, sedangkan frekuensi pergantian batang pisang dalam penelitian ini yaitu tiga hari, tujuh hari dan tanpa pergantian batang pisang. Perlakuan perendaman dengan cacahan batang pisang dilakukan selama 14 hari, kemudian dilakukan uji tantang dengan bakteri <em>S. agalactiae</em> pada hari ke-15 selama 14 hari pengamatan. Hasil penelitian menunjukkan bahwa cacahan batang pisang ambon mengandung senyawa aktif berupa alkaloid, flavonoid (17,63%), tanin (0,02%) dan saponin (0,24%). Senyawa aktif tersebut mampu meningkatkan respons imun non-spesifik pada ikan nila yaitu <em>respiratory burst</em>, aktivitas lisozim, aktivitas fagositik, total leukosit, total eritrosit dan hemoglobin. Perlakuan dengan konsentrasi 5 g/L dan tanpa pergantian cacahan batang pisang merupakan perlakuan terbaik dengan nilai <em>relative percent survival</em> (RPS) mencapai 70%, lebih besar dibandingkan dengan perlakuan lainnya. Selain itu, perlakuan ini memiliki nilai tertinggi pada aktivitas fagositik (36,6%), <em>respiratory burst</em> (0,58 pada O.D. 630 nm) dan aktivitas lisozim (72,7 Unit/mL) setelah hari ke‒4 uji tantang dengan bakteri <em>S. agalactiae</em>.</p><p align="center"><strong> </strong></p><p>Kata kunci: batang pisang, ikan nila, imunostimulan, respons imun, <em>Streptococcus agalactiae</em></p><p> </p>

Dietary supplementation of dry oregano leaves increases the innate immunity and resistance of Nile tilapia against Streptococcus agalactiae infection

2019 ◽  
Vol 51 (2) ◽  
pp. 418-436 ◽  
Author(s):  
Amanda H. Espirito Santo ◽  
Túlio S. Brito ◽  
Laura L. Brandão ◽  
Guilherme C. Tavares ◽  
Márcia P. Leibowitz ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Streptococcus Agalactiae ◽  
Nile Tilapia ◽  
Dietary Supplementation

Nonlethal sampling methods for diagnosis of Streptococcus agalactiae infection in Nile tilapia, Oreochromis niloticus (L.)

Aquaculture ◽  
2016 ◽  
Vol 454 ◽  
pp. 237-242 ◽  
Author(s):  
Guilherme Campos Tavares ◽  
Frederico Augusto de Alcântara Costa ◽  
Raquel Ribeiro Dias Santos ◽  
Gustavo Morais Barony ◽  
Carlos Augusto Gomes Leal ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Sampling Methods ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Dietary Agaricus blazei Spent Substrate Improves Disease Resistance of Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae In Vivo

2022 ◽  
Vol 10 (1) ◽  
pp. 100
Author(s):  
Po-Tsang Lee ◽  
Yu-Sheng Wu ◽  
Chung-Chih Tseng ◽  
Jia-Yu Lu ◽  
Meng-Chou Lee
Keyword(s):  
Cell Line ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Water Extract ◽  
Feed Additive ◽  
Agaricus Blazei ◽  
Bacterial Challenge ◽  
Spent Mushroom Substrate ◽  
Nile Tilapia Oreochromis Niloticus

This study evaluated the effects of the feeding of spent mushroom substrate from Agaricus blazei on Nile tilapia (Oreochromis niloticus). The safety of 0–1000 μg/mL A. blazei spent substrate water extract (ABSSE) was demonstrated in the primary hepatic and splenic macrophages and the THK cell line (a cell line with characteristics of melanomacrophages) using a cytotoxicity assay. Here, 10 μg/mL of crude ABSSE promoted the phagocytic activity of macrophages and THK cells. Stimulating ABSSE-primed THK cells with lipopolysaccharides or peptidoglycan resulted in higher expression levels of four cytokine genes (e.g., interleukinz (IL)-1β, IL-12b, IL-8 and tumor necrosis factor α (TNFα)) and one cytokine gene (TNFα), respectively. An in vitro bacterial growth inhibition assay demonstrated that ABSSE could inhibit the growth of Streptococcus agalactiae. In the first feeding trial, Nile tilapia were fed with experimental feed containing 0, 1, or 5% of A. blazei spent substrate (ABSS) for seven and fourteen days followed by bacterial challenge assay. The best result was obtained when Nile tilapia were continuously fed for seven days on a diet containing 1% ABSS, with the survival rate being higher than in groups with 0% and 5% ABSS after challenge with S. agalactiae. In the second trial, fish were fed diets supplemented with 0% or 1% ABSS for seven days, and then all the groups were given the control feed for several days prior to bacterial challenge in order to investigate the duration of the protective effect provided by ABSS. The results showed that the protective effects were sustained at day 7 after the feed was switched. Overall, spent mushroom substrate from A. blazei is a cost-effective feed additive for Nile tilapia that protects fish from S. agalactiae infection.

scholarly journals Efikasi Vaksinasi pada Benih Ikan Nila (Oreochromis niloticus) dengan Metode Infiltrasi Hiperosmotik untuk Mencegah Infeksi Streptococcus agalactiae (THE EFFICACY OF VACCINATION ON TILAPIA SEEDS OF (Oreochromis niloticus) USING HYPEROSMOTIC INFILTRATION

2018 ◽  
Vol 18 (4) ◽  
pp. 634 ◽  
Author(s):  
Amalia Putri Firdausi ◽  
Sukenda Sukenda ◽  
Sri Nuryati
Keyword(s):  
Immune System ◽  
Antibody Level ◽  
Specific Antibody ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Challenge Test ◽  
Salinity Treatment ◽  
Post Vaccination ◽  
Randomized Design ◽  
And Control

The objective of this study was to evaluate the effectiveness of vaccination on tilapia seedlings resulted from the vaccinated parent by hyperosmotic infiltration method at four different salinity to prevent Streptococcus agalactiae bacterial infection. A total of 100 seeds aged 20 days from the vaccinated mother were immersed in four different salinity: 0 ppt (control), 10 ppt, 20 ppt, and 30 ppt for five minutes, then removed and transferred into vaccine-containing containers for 30 minutes. Further maintenance was performed in freshwater aquariums and at days 10, 20, and 30 post vaccination, the immune system performance: Relative Percent Survival (RPS) after the challenge test, specific antibody level, and lysozyme was observed. The study was conducted using a complete randomized design with three replications. The results showed that the 10 ppt salinity treatment gave the best results compared to the others and control. The final RPS (10 ppt) value was 84.72%, 66.49%, and 47.06%, on the 10th, 20th, and 30thh days of vaccination, respectively. Specific antibody levels (10 ppt) were 0.077, 0.078, and 0.077 and lysozyme 0.092, 0.084, and 0.032 at days 10, 20, and 30 post vaccination, respectively. The value of RPS and specific antibody level at 10 ppt salinity was significantly different (P <0.05) compared to the other treatments, while the lysozyme treatment activity of 10 ppt was not significantly different compared to the 20 ppt treatment. Vaccination using 10 ppt saline hyperosmotic infiltration can improve the performance of the immune system by improving maternal immune protection against Streptococcus agalactiae infection.

Sign in / Sign up

Export Citation Format

Share Document