Effects of background adaptation on the skin color of Malaysian red tilapia

Aquaculture ◽  
2020 ◽  
Vol 521 ◽  
pp. 735061 ◽  
Author(s):  
Lan-mei Wang ◽  
Ming-kun Luo ◽  
Hao-ran Yin ◽  
Wen-bin Zhu ◽  
Jian-jun Fu ◽  
...  
2018 ◽  
Vol 19 (4) ◽  
pp. 1209 ◽  
Author(s):  
Lanmei Wang ◽  
Wenbin Zhu ◽  
Zaijie Dong ◽  
Feibiao Song ◽  
Juanjuan Dong ◽  
...  

Author(s):  
Lan-mei Wang ◽  
Hong-yi Bu ◽  
Fei-biao Song ◽  
Wen-bin Zhu ◽  
Jian-jun Fu ◽  
...  

2021 ◽  
Author(s):  
bingjie Jiang ◽  
lanmei Wang ◽  
mingkun Luo ◽  
jianjun Fu ◽  
wenbin Zhu ◽  
...  

Abstract The commercial value of red tilapia is hampered by variations in skin color during overwintering. In this study, three types skin of red tilapia, including the skin remained pink color during and after overwintering (WP), the skin changed from pink color to black color during overwintering and remained black color after overwintering (PB), and the skin changed from pink color to black color during overwintering but recovered to pink color when the temperature rose after overwintering (BP), were used to analyze their molecular mechanisms of color variation. The transcriptome results revealed that the PB, WP, and BP libraries had 42, 43, and 43 million clean reads, respectively. The top 10 abundance mRNAs and specific mRNAs (specificity measure SPM > 0.9) were screened. After comparing intergroup gene expression levels, there were 2528, 1924 and 1939 differentially expressed genes (DEGs) between BP and PB, BP and WP, and PB and WP, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of color-related mRNAs showed that a number of DEGs, including tyrp1, tyr, pmel, mitf, mc1r, asip, tat, hpdb and foxd3, might play a potential role in pigmentation. Additionally, the co-expression patterns of genes were detected within the pigment-related pathways by PPI network from PB_WP group. Furthermore, DEGs from the apoptosis and autophagy pathways, such as baxα, beclin1, and atg7, might be involved in the fading of red tilapia melanocytes. The findings will aid in understanding the molecular mechanism underlying skin color variation in red tilapia during and after overwintering, as well as lay a foundation for future research aimed at improving red tilapia skin color characteristics.


2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Wenbin Zhu ◽  
Lanmei Wang ◽  
Zaijie Dong ◽  
Xingting Chen ◽  
Feibiao Song ◽  
...  

Abstract Red tilapia is becoming more popular for aquaculture production in China in recent years. However, the pigmentation differentiation in genetic breeding is the main problem limiting its development of commercial red tilapia culture and the genetic basis of skin color variation is still unknown. In this study, we conducted Illumina sequencing of transcriptome on three color variety red tilapia. A total of 224,895,758 reads were generated, resulting in 160,762 assembled contigs that were used as reference contigs. The contigs of red tilapia transcriptome had hits in the range of 53.4% to 86.7% of the unique proteins of zebrafish, fugu, medaka, three-spined stickleback and tilapia. And 44,723 contigs containing 77,423 simple sequence repeats (SSRs) were identified, with 16,646 contigs containing more than one SSR. Three skin transcriptomes were compared pairwise and the results revealed that there were 148 common significantly differentially expressed unigenes and several key genes related to pigment synthesis, i.e. tyr, tyrp1, silv, sox10, slc24a5, cbs and slc7a11, were included. The results will facilitate understanding the molecular mechanisms of skin pigmentation differentiation in red tilapia and accelerate the molecular selection of the specific strain with consistent skin colors.


2020 ◽  
Author(s):  
Jinzhao He ◽  
Pengfei Feng ◽  
Junqi Qin ◽  
Yun Teng ◽  
Xu Luo ◽  
...  

Abstract Background This study aimed to investigate the genetic molecular mechanism of body color differentiation and variation of red tilapia, selecting the main genes related to the variation and cultivating the pure and stable red tilapia variety. Results The effects of different temperature treatments on body color and survival of Guam red tilapia, pearl white red tilapia and Florida red tilapia were compared. Besides, comparative transcriptome analysis was used to screen the candidate genes linked to the skin color differentiation of pearl white red tilapia. Among them, the body color of Guam red tilapia changed when the water temperature dropped to 16 − 14 °C, and continued to drop to about 11 °C, it was discolored in a large area reaching above 90%. According to the differential analysis: Tyrosine Kinase STYK1, HSP70, HSP30 and Transcription factor Sp6 expressions were significantly increased in the low temperature group, while MC1R, Transcription factor (MafB, jun-D, AP-1, E2F5, ETV6, Sp9, Sp7, E2F1, Sp4) expressions were notably decreased. Conclusions In this study, the expressive quantity of tyrosine protein kinase in the low temperature group HLF7 was significantly higher than that in the normal temperature group HLF3, which indicated that the melanin synthesis ability was enhanced in the low temperature group HLF7. The result was consistent with the tendency of red tilapia to become darker as the temperature gradient decreased.


1998 ◽  
Vol 159 (2) ◽  
pp. 281-286 ◽  
Author(s):  
CH Dotman ◽  
F van Herp ◽  
GJ Martens ◽  
BG Jenks ◽  
EW Roubos

The toad Xenopus laevis is able to adapt its skin color to background light intensity. In this neuroendocrine reflex, the proopiomelanocortin (POMC)-derived peptide alpha-melanophore-stimulating hormone (alphaMSH) is a key regulatory factor. In animals adapting to a black background, release of alphaMSH from the pituitary pars intermedia causes dispersal of melanin in skin melanophores. To investigate the long-term in vivo dynamics of alphaMSH production during black background adaptation, the biosynthetic rate of POMC and the contents of POMC, alphaMSH and the POMC processing enzyme precursor convertase 2 (PC2) have been studied in the pars intermedia using pulse-labeling, Western blot and radioimmunoassay. In control animals, adapted to a white background, the rate of POMC biosynthesis and the POMC content were low, while high alphaMSH and PC2 contents were found. After 1 week of adaptation to a black background, the rate of POMC biosynthesis and the POMC protein content had increased 19- and 3.7-fold respectively. These parameters attained a maximum level (28- and 5. 8-fold higher than control) after 3 weeks and remained at these elevated levels for at least 12 weeks. After 1 week, the pars intermedia content of alphaMSH was only 30% of the control level, but after 6 and 12 weeks, the alphaMSH level had increased to the control level. The PC2 content decreased to 52% of control after 1 week and stabilized after 3 weeks at a level slightly lower than the control value. The results show that during long-term background adaptation a steady-state situation is reached, with a balance between the biosynthesis, enzymatic processing and release of alphaMSH. The in vivo dynamics of the processing enzyme PC2 suggest a parallel storage and release of alphaMSH and mature PC2 in the Xenopus pituitary pars intermedia.


2022 ◽  
Vol 22 ◽  
pp. 100946
Author(s):  
Lan-mei Wang ◽  
Bing-jie Jiang ◽  
Wen-bin Zhu ◽  
Jian-jun Fu ◽  
Ming-kun Luo ◽  
...  

Aquaculture ◽  
2018 ◽  
Vol 490 ◽  
pp. 149-155 ◽  
Author(s):  
Lan-mei Wang ◽  
Wen-bin Zhu ◽  
Jie Yang ◽  
Ling-hong Miao ◽  
Juan-juan Dong ◽  
...  
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