Anti-parasitic effect of lacquer tree Rhus verniciflua Stokes lignum against Miamiensis avidus (Ciliophora: Scuticociliatida)

Abstract The Rhus verniciflua Stokes (RVS) extract is used as a traditional herbal medicine in Southeast Asian countries such as Korea and China. In the present study, one phenolic acid and six flavonoids were isolated from an 80% ethanol RVS extract to examine their antimicrobial activities. These compounds were identified as 3′,4′,7-trihydroxyflavone (1), methyl gallate (2), gallic acid (3), fusti (4), fisetin (5), butin (6), and sulfuretin (7) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and nuclear magnetic resonance spectroscopy. The antimicrobial activities of compounds 5 and 6 (at a dose of 16 μg/mL each) were superior to that of the control, cycloheximide (at a dose of 25 μg/mL), against Hypocrea nigricans; additionally, the activities of compounds 1 and 2 (at a dose of 8 μg/mL each) were superior to the control against Penicillium oxalicum. Also, chemical compounds 1 and 5 (at a dose of 16 μg/mL each) had higher activities than the control (25 μg/mL) against Trichoderma virens. Chemical compound 1 (at a dose of 8 μg/mL) had a similar activity to that of the control against Bacillus subtilis. The obtained results suggest that the RVS extract could be a promising food and nutraceutical source because of the antimicrobial properties of its phenolic compounds.

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Rhus verniciflua Stokes Extract and Its Flavonoids Protect PC-12 Cells against H2O2-Induced Cytotoxicity

Journal of Microbiology and Biotechnology ◽

10.4014/jmb.1612.12018 ◽

2017 ◽

Vol 27 (6) ◽

pp. 1090-1097 ◽

Cited By ~ 5

Author(s):

Tae Gyu Nam ◽

Bong Han Lee ◽

Hyo-Kyoung Choi ◽

Ahmad Rois Mansur ◽

Sang Gil Lee ◽

...

Keyword(s):

Pc 12 Cells ◽

Rhus Verniciflua Stokes ◽

Rhus Verniciflua

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Flavonoids purified from Rhus verniciflua Stokes actively inhibit cell growth and induce apoptosis in human osteosarcoma cells

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/j.bbagen.2005.08.010 ◽

2005 ◽

Vol 1726 (3) ◽

pp. 309-316 ◽

Cited By ~ 89

Author(s):

Hyon-Seok Jang ◽

Sung-Ho Kook ◽

Young-Ok Son ◽

Jong-Ghee Kim ◽

Young-Mi Jeon ◽

...

Keyword(s):

Cell Growth ◽

Osteosarcoma Cells ◽

Human Osteosarcoma ◽

Rhus Verniciflua Stokes ◽

Rhus Verniciflua ◽

Human Osteosarcoma Cells ◽

Inhibit Cell Growth

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Extract of Rhus verniciflua Stokes Induces p53-Mediated Apoptosis in MCF-7 Breast Cancer Cells

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/9407340 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Min Sung Kim ◽

Chul Won Lee ◽

Jung-Hoon Kim ◽

Jang-Cheon Lee ◽

Won Gun An

Keyword(s):

Breast Cancer ◽

High Performance ◽

Annexin V ◽

Food Supplement ◽

Dependent Manner ◽

Related Factors ◽

Rhus Verniciflua Stokes ◽

Rhus Verniciflua ◽

Dose Dependent ◽

Mcf 7

Rhus verniciflua Stokes has long been used as a food supplement and traditional herbal medicine for various ailments in East Asia. We evaluated the anticancer effects of Rhus verniciflua Stokes extract (RVSE) on MCF-7 cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, annexin V/7-AAD staining, and western blotting. In addition, the gallic acid content of RVSE was assayed using high-performance liquid chromatography. RVSE inhibited the growth of MCF-7 cells in a dose-dependent manner by inducing apoptosis in the sub-G1 phase. RVSE also significantly increased the number of apoptotic cells and increased the expression of p53 and p21 in a dose-dependent manner. Furthermore, RVSE treatment increased the Bax:Bcl-2 ratio and the levels of apoptosis-related factors, such as cleaved caspase-3 and -9 and PARP, in MCF-7 cells. Our findings suggest that the proapoptotic effect of RVSE on MCF-7 cells is mediated by p53, p21, and the intrinsic mitochondrial cascade. Thus, RVSE shows promise for the prevention and treatment of breast cancer.

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