Comparison of oxidative stress induced by clarithromycin in two freshwater microalgae Raphidocelis subcapitata and Chlorella vulgaris

Abstract Two model algae Chlorella vulgaris (C. vulgaris) and Raphidocelis subcapitata (R. subcapitata) were generally used to test chemicals with antimicrobial properties during registration process. However, it has been reported that significant sensitivity difference in two algae when exposure to antibiotics. Furthermore, the selection of an appropriate test species play a vital role in evaluate of environmental hazards and risks of compounds. Since the balance between oxidative stress and antioxidant is a crucial factor on alga growth. This experiment is performed to investigate the working of oxidative stress and mechanism of antioxidant defense system of algae under antibiotic stress. A series of concentration of Tylosin (TYN), a macrolide antibiotic, were used to test in this study. Oxidative stress biomarkers (Malondialdehyde (MDA)), non-enzymatic antioxidants (Reduced glutathione (GSH)), antioxidant enzymes (Superoxide dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GP), Glutathione S-transferase (GST)) and photosynthetic pigments were measured to investigate antioxidant defense system. R. subcapitata was significantly inhibited with increasing concentration of TYN, whereas no effects on C. vulgaris. The contents of MDA increased significantly when species were inhibited, and thus, activating the antioxidant system, companying with the significantly increasing of SOD and CAT.

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Chlorella vulgaris ameliorates testicular toxicity induced by deltamethrin in male rats via modulating oxidative stress

Andrologia ◽

10.1111/and.13214 ◽

2018 ◽

Vol 51 (3) ◽

pp. e13214 ◽

Cited By ~ 3

Author(s):

Eman Osama ◽

Azza A. A. Galal ◽

Hany Abdalla ◽

Sawsan M. A. El-Sheikh

Keyword(s):

Oxidative Stress ◽

Chlorella Vulgaris ◽

Male Rats ◽

Testicular Toxicity

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Evaluation of the toxicity of herbicide topramezone to Chlorella vulgaris : Oxidative stress, cell morphology and photosynthetic activity

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2017.05.022 ◽

2017 ◽

Vol 143 ◽

pp. 129-135 ◽

Cited By ~ 15

Author(s):

Fangfang Zhao ◽

Qingqing Xiang ◽

Ying Zhou ◽

Xiao Xu ◽

Xinyi Qiu ◽

...

Keyword(s):

Oxidative Stress ◽

Chlorella Vulgaris ◽

Cell Morphology ◽

Photosynthetic Activity

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PHYSIOLOGICAL AND BIOCHEMICAL CHANGES IN ALGAL CULTURES OF CHLORELLA VULGARIS AND SYNECHOCYSTIS SALINA (MESOPHILIC AND ANTARCTIC ISOLATES) OCCURING AFTER TREATMENT WITH UV-B RADIATION

10.32006/eeep.2017.1.7382 ◽

2017 ◽

pp. 73-82

Author(s):

Dilyana Doneva ◽

Juliana Ivanova ◽

Lyudmila Kabaivanova

Keyword(s):

Oxidative Stress ◽

Chlorophyll A ◽

Chlorella Vulgaris ◽

Markers Of Oxidative Stress ◽

Protective Strategies ◽

The Antarctic ◽

Antarctic Algae ◽

Β Carotene ◽

Stimulation Of

Determination of biomass production and viability of algal cells of Chlorella vulgaris and Synechocystis salina exposed to UV-B radiation were carried out in this study together with comparison of the mesophilic and antarctic isolates of both investigated strains. Estimation of the content of the pigments: chlorophyll a, chlorophyll b, β-carotene, C-phycocyanin and allo-phycocyanin in algal cells exposed to UV-B radiation was also accomplished. The obtained results showed that the antarctic algae are more resistant to oxidative stress than their mesophilic counterparts. The antarctic isolates of Ch. vulgaris and S. salina compared with the mesophilic ones - up to 72 h showed tolerance to low exposures of radiation, expressed in a slight stimulation of growth and viability of the cells. Antarctic isolates also showed greater resistance to low doses of UV-B radiation manifested by stimulation of the synthesis of chlorophyll a and β-carotene. The registered increase in the amount of C- and allo-phycocyanin in antarctic isolates of S. salina showed that they had developed protective strategies against UV-B radiation by increasing the concentration of the phycobiliproteins. As a result of increased UV-B background, in antarctic isolates, stronger antioxidant defence mechanisms are triggered, which proved the possibility of using them as markers of oxidative stress.

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Chlorella vulgaris Ameliorates Oxidative Stress and Improves the Muscle Regenerative Capacity of Young and Old Sprague-Dawley Rats

Nutrients ◽

10.3390/nu12123752 ◽

2020 ◽

Vol 12 (12) ◽

pp. 3752

Author(s):

Nurhazirah Zainul Azlan ◽

Yasmin Anum Mohd Yusof ◽

Suzana Makpol

Keyword(s):

Oxidative Stress ◽

Muscle Mass ◽

Chlorella Vulgaris ◽

Untreated Control ◽

Oxidative Stress Marker ◽

Sprague Dawley ◽

Old Rats ◽

Group 2 ◽

And Function ◽

Gastrocnemius Muscles

Muscle atrophy in ageing is a multifactorial degenerative process impacted by cellular ageing biology, which includes oxidative stress. Chlorella vulgaris is a coccoid green eukaryotic microalga rich in antioxidants. The aim of this study was to determine the effect of C. vulgaris in ameliorating oxidative stress, thus elucidating its mechanism in improving muscle mass, strength and function in young and old rats. Fifty-six male Sprague-Dawley (SD) rats aged 3 months (young) and 21 months (old) were divided into three groups: Group 1 (control) was given distilled water; Group 2 was treated with 150 mg/kg body weight (BW) of C. vulgaris; and Group 3 was treated with 300 mg/kg BW of C. vulgaris for three months. Grip and muscle strength and muscle integrity were determined on days 0, 30, 60, and 90 of treatment. Urine and blood were collected on days 0 and 90 of treatment for oxidative stress marker determination, while the gastrocnemius muscles were collected for muscle oxidative stress analysis. Increased grip strength of the front and hind paws was observed in young C. vulgaris-treated rats on days 30, 60, and 90 compared to the untreated control on the same days (p < 0.05). There was a significant increase in lean bone mineral content (BMC) in young rats treated with 300 mg/kg BW C. vulgaris compared to untreated rats on days 30 and 60. The fat mass was significantly decreased in young and old C. vulgaris-treated rats on day 90 compared to the untreated control. The total path was significantly increased for old rats treated with 300 mg/kg BW C. vulgaris on days 60 and 90 compared to day 0. Young and old C. vulgaris-treated rats demonstrated a significant decrease in urinary isoprostane F2t and plasma creatine kinase-MM (CKMM) compared to the control on day 90. A significant decrease in malondialdehyde (MDA) and 4-hydroxyalkenal (HAE) levels were observed in young and old rats treated with C. vulgaris. C. vulgaris improved the muscle mass, strength, and function in young and old rats. This effect could be due to its potency in ameliorating oxidative stress in the skeletal muscle of young and old rats.

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The Extract of Chlorella vulgaris Protects Transformed Retinal Ganglion Cells from Oxidative Stress-induced Cells Death

Journal of Food Biochemistry ◽

10.1111/jfbc.12030 ◽

2013 ◽

Vol 38 (2) ◽

pp. 129-139 ◽

Cited By ~ 2

Author(s):

Kyung-A Kim ◽

Kwang Hyun Cha ◽

Soon-Jung Choi ◽

Cheol-Ho Pan ◽

Sang Hoon Jung

Keyword(s):

Oxidative Stress ◽

Retinal Ganglion Cells ◽

Chlorella Vulgaris ◽

Ganglion Cells ◽

Retinal Ganglion ◽

Cells Death

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Biotoxicity of degradable carbon dots towards microalgae Chlorella vulgaris

Environmental Science Nano ◽

10.1039/c9en00829b ◽

2019 ◽

Vol 6 (11) ◽

pp. 3316-3323 ◽

Cited By ~ 3

Author(s):

Mengling Zhang ◽

Huibo Wang ◽

Peipei Liu ◽

Yuxiang Song ◽

Hui Huang ◽

...

Keyword(s):

Oxidative Stress ◽

Chlorella Vulgaris ◽

Carbon Dots ◽

Rubisco Activity

The CDs inhibit the growth of Chlorella vulgaris through triggering oxidative stress, decreasing the Rubisco activity and inhibiting the transcription of photosynthesis-related genes.

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