Effect of Inula viscosa on the pellicle’s protective properties and initial bioadhesion in-situ

2016 ◽  
Vol 71 ◽  
pp. 87-96 ◽  
Author(s):  
Susann Hertel ◽  
Leif Graffy ◽  
Sandra Pötschke ◽  
Sabine Basche ◽  
Ali Al-Ahmad ◽  
...  
2015 ◽  
Vol 80 (5) ◽  
pp. 705-715 ◽  
Author(s):  
Milica Milosevic ◽  
Ana Krkobabic ◽  
Marija Radoicic ◽  
Zoran Saponjic ◽  
Vesna Lazic ◽  
...  

The possibility of in situ photoreduction of Ag+ ions using colloidal TiO2 nanoparticles deposited on the surface of polyamide fabric in the presence of amino acid alanine and methyl alcohol is discussed. The presence of TiO2/Ag nanoparticles on the polyamide fabric was confirmed by FESEM and ICP analyses. Antibacterial activity of the fabric was tested against Gram-negative bacterium Escherichia coli and Gram-positive bacterium Staphylococcus aureus. Fabricated TiO2/Ag nanoparticles on the surface of polyamide fabric provided maximum bacterial reduction and thus, excellent antibacterial activity. In spite of silver leaching from the fabric during washing, impregnated polyamide fabric preserved maximum reduction of Escherichia coli colonies. Antibacterial activity against Staphylococcus aureus slightly decreased after ten washing cycles, but still antibacterial activity can be considered as satisfactory. In addition, the presence of TiO2/Ag nanoparticles ensured better UV protection efficiency which belongs to very good UV protection category.


2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
A. Kensche ◽  
S. Pötschke ◽  
C. Hannig ◽  
G. Richter ◽  
W. Hoth-Hannig ◽  
...  

For the purpose of erosion prevention the present study aimed to compare the efficacy of two biomimetic products and a fluoride solution to optimize the protective properties of the pellicle. After 1 min ofin situpellicle formation on bovine enamel slabs, 8 subjects adopted CPP-ACP (GC Tooth Mousse), a mouthwash with hydroxyapatite microclusters (Biorepair), or a fluoride based mouthwash (elmex Kariesschutz) for 1 min each. Afterwards, samples were exposed in the oral cavity for 28 min. Native enamel slabs and slabs exposed to the oral cavity for 30 min without any rinse served as controls. After oral exposure, slabs were incubated in HCl (pH values 2, 2.3, and 3) for 120 s and kinetics of calcium and phosphate release were measured photometrically; representative samples were evaluated by SEM and TEM. The physiological pellicle reduced demineralization at all pH values; the protective effect was enhanced by fluoride. The biomimetic materials also reduced ion release but their effect was less pronounced. SEM indicated no layer formation after use of the different products. However, TEM confirmed the potential accumulation of mineral components at the pellicle surface. The tested products improve the protective properties of thein situpellicle but not as effectively as fluorides.


2015 ◽  
Vol 49 (5) ◽  
pp. 477-487 ◽  
Author(s):  
Marie-Theres Weber ◽  
Matthias Hannig ◽  
Sandra Pötschke ◽  
Franziska Höhne ◽  
Christian Hannig

Objectives: Antiadherent and antibacterial effects of certain plant extracts have been proven to be beneficial in preventive dentistry. In the present in situ/in vitro crossover study, the impact of plant extracts rich in polyphenols on the erosion-protective properties of the in situ pellicle was evaluated. Methods: Individual splints were prepared for 12 subjects for intraoral exposure of bovine enamel specimens. Following formation of a 1-min pellicle, watery plant extracts (leaves of the wild form of Ribes nigrum, the wild form of Origanum as well as a combination of both) were administered for 10 min in situ. Alternatively, a mouth rinse with fluorides (Elmex Kariesschutz) was performed for 1 min. After further oral exposure for 19/28 min, respectively, slabs were removed and incubated with HCl in vitro over 120 s (pH 2, 2.3, 3). The resulting calcium and phosphate release was quantified photometrically. Slabs with and without a 30-min in situ pellicle served as controls. The modification of pellicle ultrastructure was evaluated by transmission electron microscopy (TEM). Results: Plant extracts modulated the erosion-protective properties of the native in situ pellicle in all test groups in a pH-dependent manner. The combination of R. nigrum leaves and Origanum enhanced the protective properties of the pellicle at all pH values; the administration of this preparation was comparable, yet superior, to the effect of the fluoridated mouth rinse. TEM images indicated that rinsing with R. nigrum leaves/Origanum yielded a distinctly thicker and more electron-dense pellicle. Conclusion: The combination of certain plant extracts offers a novel approach to the complementary prevention of dental erosion.


Life ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 56 ◽  
Author(s):  
Teresa Fornaro ◽  
Andrew Steele ◽  
John Brucato

Minerals might have played critical roles for the origin and evolution of possible life forms on Mars. The study of the interactions between the “building blocks of life” and minerals relevant to Mars mineralogy under conditions mimicking the harsh Martian environment may provide key insight into possible prebiotic processes. Therefore, this contribution aims at reviewing the most important investigations carried out so far about the catalytic/protective properties of Martian minerals toward molecular biosignatures under Martian-like conditions. Overall, it turns out that the fate of molecular biosignatures on Mars depends on a delicate balance between multiple preservation and degradation mechanisms, often regulated by minerals, which may take place simultaneously. Such a complexity requires more efforts in simulating realistically the Martian environment in order to better inspect plausible prebiotic pathways and shed light on the nature of the organic compounds detected both in meteorites and on the surface of Mars through in situ analysis.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
A. Kensche ◽  
E. Buschbeck ◽  
B. König ◽  
M. Koch ◽  
J. Kirsch ◽  
...  

2012 ◽  
Vol 46 (5) ◽  
pp. 496-506 ◽  
Author(s):  
C. Hannig ◽  
C. Wagenschwanz ◽  
S. Pötschke ◽  
K. Kümmerer ◽  
A. Kensche ◽  
...  

2016 ◽  
Vol 51 (1) ◽  
pp. 34-45 ◽  
Author(s):  
Susann Hertel ◽  
Sandra Pötschke ◽  
Sabine Basche ◽  
Judith Delius ◽  
Wiebke Hoth-Hannig ◽  
...  

Objectives: In the present in situ/ex vivo study the impact of tannic acid on the erosion-protective properties of the enamel pellicle was tested. Additionally, the antiadherent and antibacterial effects of tannic acid were evaluated. Methods: The pellicle was formed in situ on bovine enamel samples fixed on individual splints worn by 6 subjects. Following 1 min of pellicle formation the volunteers rinsed for 10 min with tannic acid. After further oral exposure for 19 min, 109 min, and 8 h overnight, respectively, slabs were incubated in HCl ex vivo (pH 2.0, 2.3, 3.0) over 120 s. Subsequently, kinetics of calcium and phosphate release were measured photometrically. Samples after a 1-min fluoride mouth rinse as well as enamel samples with and without a 30-min in situ pellicle served as controls. Antiadherent effects were evaluated after a 1-min rinse with tannic acid and oral exposure of the slabs overnight. DAPI (4′,6-diamidino-2-phenylindole) combined with concanavalin A staining and live/dead staining was used for fluorescence microscopic visualization and quantification of adherent bacteria and glucans. Modification of the pellicle's ultrastructure by tannic acid was evaluated by transmission electron microscopy (TEM). Results: Tannic acid significantly improved the erosion-protective properties of the pellicle in a pH-dependent manner. Bacterial adherence and glucan formation on enamel were significantly reduced after rinses with tannic acid as investigated by fluorescence microscopy. TEM imaging indicated that rinsing with tannic acid yielded a sustainable modification of the pellicle; it was distinctly more electron dense. Conclusion: Tannic acid offers an effective and sustainable approach for the prevention of caries and erosion.


2017 ◽  
Vol 7 (1) ◽  
Author(s):  
A. Kensche ◽  
J. Kirsch ◽  
S. Mintert ◽  
F. Enders ◽  
S. Pötschke ◽  
...  

Author(s):  
Teresa Fornaro ◽  
Andrew Steele ◽  
John Robert Brucato

Minerals might have played critical roles for the origin and evolution of possible life forms on Mars. The study of the interactions between “building blocks of life” and minerals relevant to Mars mineralogy under conditions mimicking the harsh Martian environment may provide key insight into possible prebiotic processes. Therefore, this contribution aims at reviewing the most important investigations carried out so far about the catalytic/protective properties of Martian minerals toward molecular biosignatures under Martian-like conditions. Overall, it turns out that the fate of molecular biosignatures on Mars depends on a delicate balance between multiple preservation and degradation mechanisms often regulated by minerals, which may take place simultaneously. Such a complexity requires more efforts in simulating realistically the Martian environment in order to better inspect plausible prebiotic pathways and shed light on the nature of the organic compounds detected both in meteorites and on the surface of Mars through in situ analysis.


2020 ◽  
Vol 4 (3) ◽  
pp. 305-337

The aim of this review is to analyze the existing approaches to the creation and the improvement of materials and fabrics based on metal-organic frameworks (MOFs), which can be the basis for developing a strategy for creating modular MOF materials for modern personal protective equipment (PPE) against toxic chemicals and pathogenic microorganisms. The review provides the details of the use of biomolecules for integration with MOF by pore encapsulation, surface attachment, covalent binding, in situ encapsulation of biomolecules in MOF, and the creation of bio-MOFs. The characteristics of biomolecules that can be included in MOFs are presented. Certain schemes for the inclusion of some biomolecules in MOFs are given. The main properties of MOFs, the main trends of their creation, as well as the most promising directions of application of biomolecules/MOF composites are discussed. The strategy of the creation of modular MOF materials with desired properties for modern PPE, that provide protection against various hazards of chemical, biological and physical nature, is presented. The main trends of the development of modern PPE based on modular MOF materials with desired properties are given. Particular schemes of the creation of separate modules in MOF structures, as well as the determination of their importance and significance for imparting universal protective properties to MOF materials are proposed


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