Effect of selected B-ring-substituted oxysterols on artificial model erythrocyte membrane and isolated red blood cells

Key PointsInhibitors of human Syk kinase suppress parasite egress. Syk inhibitors prevent the tyrosine phosphorylation of band 3 in P falciparum parasitized red blood cells, reducing the release of microparticles.

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A Numerical Study on Drug Delivery via Multiscale Synergy of Cellular Hitchhiking onto Red Blood Cells

Nanoscale ◽

10.1039/d1nr04057j ◽

2021 ◽

Author(s):

Mehdi Nikfar ◽

Meghdad Razizadeh ◽

Ratul Paul ◽

Vladimir N Muzykantov ◽

Yaling Liu

Keyword(s):

Drug Delivery ◽

Blood Cell ◽

Red Blood Cells ◽

Erythrocyte Membrane ◽

Intravenous Injection ◽

Red Blood Cell ◽

Blood Cells ◽

Numerical Study

Red blood cell (RBC)-hitchhiking, in which different nanocarriers (NCs) shuttled on the erythrocyte membrane and disassociated from RBCs to the first organ downstream of the intravenous injection spot, has recently...

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Targeted mutagenesis of Plasmodium falciparum erythrocyte membrane protein 3 (PfEMP3) disrupts cytoadherence of malaria-infected red blood cells

The EMBO Journal ◽

10.1093/emboj/19.12.2813 ◽

2000 ◽

Vol 19 (12) ◽

pp. 2813-2823 ◽

Cited By ~ 113

Author(s):

J. G. Waterkeyn

Keyword(s):

Plasmodium Falciparum ◽

Membrane Protein ◽

Red Blood Cells ◽

Erythrocyte Membrane ◽

Blood Cells ◽

Targeted Mutagenesis ◽

Erythrocyte Membrane Protein ◽

Falciparum Erythrocyte Membrane Protein

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Ca2+ Binding and Membrane Fluidity in Essential and Renal Hypertension

Clinical Science ◽

10.1042/cs0630281 ◽

1982 ◽

Vol 63 (3) ◽

pp. 281-284 ◽

Cited By ~ 61

Author(s):

Sergei N. Orlov ◽

Yuvenali V. Postnov

Keyword(s):

Essential Hypertension ◽

Red Blood Cells ◽

Membrane Fluidity ◽

Lipid Bilayer ◽

Erythrocyte Membrane ◽

Blood Cells ◽

Membrane Structure ◽

Renal Hypertension ◽

Lateral Diffusion ◽

Binding Ability

1. Ca2+-binding ability and membrane structure of red blood cells of patients with essential and renal hypertension were studied. 2. Ca2+-binding ability of the erythrocyte membrane of patients with essential hypertension was found to be reduced by 30% compared with that of normotensive controls. 3. The rate of lateral diffusion of pyrene in the erythrocyte membrane of patients with essential hypertension was reduced both in the lipid bilayer and in the region of annular lipid compared with that of normotensive patients. 4. There are no differences either in Ca2+-binding ability or in fluidity of the erythrocyte membrane of patients with chronic renal hypertension compared with that of normotensive individuals.

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Erythrocyte-Membrane-Enveloped Perfluorocarbon as Nanoscale Artificial Red Blood Cells to Relieve Tumor Hypoxia and Enhance Cancer Radiotherapy

Advanced Materials ◽

10.1002/adma.201701429 ◽

2017 ◽

Vol 29 (35) ◽

pp. 1701429 ◽

Cited By ~ 184

Author(s):

Min Gao ◽

Chao Liang ◽

Xuejiao Song ◽

Qian Chen ◽

Qiutong Jin ◽

...

Keyword(s):

Red Blood Cells ◽

Erythrocyte Membrane ◽

Blood Cells ◽

Tumor Hypoxia ◽

Cancer Radiotherapy

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Coherent and monochromatic irradiation changes the erythrocyte hemolysis time and the speed of the ion counter transport through the erythrocyte membrane

Energy and automation ◽

10.31548/energiya2020.03.109 ◽

2020 ◽

pp. 109-127

Author(s):

O. B. Almazova ◽

◽

M. L. Lysychenko ◽

Keyword(s):

Ion Transport ◽

Red Blood Cells ◽

Erythrocyte Membrane ◽

Blood Cells ◽

The Body ◽

Effective Thickness ◽

Blood Samples ◽

Low Intensity ◽

Counter Ion ◽

Intensity Laser

The erythrocytes of human blood were sequentially irradiated with a low-intensity laser (λ = 640 nm), a violet LED (λ = 400 nm), a green LED (λ = 540 nm), and a yellow LED (λ = 592 nm). The method of acid (chemical) erythrograms and the method of counter ion transport compared the kinetic characteristics in irradiated and unirradiated blood samples. Received: - by the method of acid erythrograms it was found that in the irradiated blood samples there is a decrease in the time of hemolysis; - low intensity laser radiation, as well as the emission of LEDs, increase the rate of counter ion transport through red blood cells. - a decrease in the time of hemolysis and an increase in the rate of counter ion transport of irradiated blood samples is due to a decrease in the "effective" thickness of the near-membrane diffusion layer - an immiscible layer of water adjoined to the erythrocyte membrane. A decrease in the "effective” thickness of the near-membrane water layer (minimal in the wavelength range of 570-590 nm and 630-640 nm) changes the rate of metabolic processes in the "cell - intercellular medium" system, changing the mode of cell functioning. The altered mode of functioning is a biological response to light radiation. Red blood cells with an altered mode of functioning are signals - stimuli that cause the body to mobilize resources to fight pathology. These circumstances can predict the creation of a universal phototherapeutic equipment for extracorporeal blood irradiation based on light-emitting diodes with certain exposure parameters.

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Photodynamic damage of the erythrocyte membrane caused by protoporphyrin in protoporphyria and in normal red blood cells

Clinica Chimica Acta ◽

10.1016/0009-8981(72)90312-9 ◽

1972 ◽

Vol 39 (1) ◽

pp. 161-170 ◽

Cited By ~ 77

Author(s):

A.A. Schothorst ◽

J. Van Steveninck ◽

L.N. Went ◽

D. Suurmond

Keyword(s):

Red Blood Cells ◽

Erythrocyte Membrane ◽

Blood Cells

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Stochastic bond dynamics facilitates alignment of malaria parasite at erythrocyte membrane upon invasion

10.1101/2020.03.01.971986 ◽

2020 ◽

Author(s):

Sebastian Hillringhaus ◽

Anil K. Dasanna ◽

Gerhard Gompper ◽

Dmitry A. Fedosov

Keyword(s):

Red Blood Cells ◽

Erythrocyte Membrane ◽

Blood Cells ◽

Malaria Parasite ◽

Invasion Process ◽

Random Orientation ◽

Hydrodynamic Simulations ◽

Stochastic Nature ◽

Rbc Membrane ◽

Alignment Process

Malaria parasites invade healthy red blood cells (RBCs) during the blood stage of the disease. Even though parasites initially adhere to RBCs with a random orientation, they need to align their apex toward the membrane in order to start the invasion process. Using hydrodynamic simulations of a RBC and parasite, where both interact through discrete stochastic bonds, we show that parasite alignment is governed by the combination of RBC membrane deformability and dynamics of adhesion bonds. The stochastic nature of bond-based interactions facilitates a diffusive-like re-orientation of the parasite at the RBC membrane, while RBC deformation aids in the establishment of apexmembrane contact through partial parasite wrapping by the membrane. This bond-based model for parasite adhesion quantitatively captures alignment times measured experimentally and demonstrates that alignment times increase drastically with increasing rigidity of the RBC membrane. Our results suggest that the alignment process is mediated simply by passive parasite adhesion.

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Ligation of complement receptor 1 increases erythrocyte membrane deformability

Blood ◽

10.1182/blood-2010-04-273904 ◽

2010 ◽

Vol 116 (26) ◽

pp. 6063-6071 ◽

Cited By ~ 26

Author(s):

Aleksandra M. Glodek ◽

Rossen Mirchev ◽

David E. Golan ◽

Joseph A. Khoory ◽

Jennie M. Burns ◽

...

Keyword(s):

Red Blood Cells ◽

Erythrocyte Membrane ◽

Immune Complexes ◽

Blood Cells ◽

Specific Inhibitor ◽

Complement Receptor ◽

Immune Adherence ◽

Rbc Membrane ◽

Complement Receptor 1 ◽

Trpc1 Channels

AbstractMicrobes as well as immune complexes and other continuously generated inflammatory particles are efficiently removed from the human circulation by red blood cells (RBCs) through a process called immune-adherence clearance. During this process, RBCs use complement receptor 1 (CR1, CD35) to bind circulating complement-opsonized particles and transfer them to resident macrophages in the liver and spleen for removal. We here show that ligation of RBC CR1 by antibody and complement-opsonized particles induces a transient Ca++ influx that is proportional to the RBC CR1 levels and is inhibited by T1E3 pAb, a specific inhibitor of TRPC1 channels. The CR1-elicited RBC Ca++ influx is accompanied by an increase in RBC membrane deformability that positively correlates with the number of preexisting CR1 molecules on RBC membranes. Biochemically, ligation of RBC CR1 causes a significant increase in phosphorylation levels of β-spectrin that is inhibited by preincubation of RBCs with DMAT, a specific casein kinase II inhibitor. We hypothesize that the CR1-dependent increase in membrane deformability could be relevant for facilitating the transfer of CR1-bound particles from the RBCs to the hepatic and splenic phagocytes.

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Impact of oxygen toxic action on the erythrocyte membrane and possibility of estimating central nervous system function disturbances

Vojnosanitetski pregled ◽

10.2298/vsp1107539b ◽

2011 ◽

Vol 68 (7) ◽

pp. 539-543 ◽

Cited By ~ 1

Author(s):

Branislava Belic ◽

Marko Cincovic

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Red Blood Cells ◽

Erythrocyte Membrane ◽

Hyperbaric Oxygen ◽

Blood Cells ◽

Hyperbaric Oxygenation ◽

Prolonged Exposure ◽

Regression Equations ◽

Membrane Rupture

Background/Aim. Prolonged exposure to hyperbaric oxygen leads to changes of erythrocytes shape as a consequence of toxic effects of oxygen on the erythrocyte membrane. The aim of this study was to examine the association between occurance of pathological forms of erythrocytes at different time from the start of hyperbaric oxygenation and the moment of convulsions occurrence, an interrelationship of different pathological forms of erythrocytes during exposure to hyperbaric oxygenation, as well as the correlation between the presence of ruptured erythrocytes and function of central nervous system (CNS) after completion of hyperbaric treatment. Methods. Sixty laboratory mice, Mus musculus, were exposed to the wholly-oxygen pressure of 3.5 absolute atmospheres (ATA). Blood was collected at the 32nd, 34th, 36th, 38th and 40th minutes after the exposure to oxygen. Pathological forms of erythrocytes were examined by electron microscopy. A moment of convulsions occurrence was registered in all animals. After decompression neurological examinations of experimental animals were perfomed. The Pearson's coefficient of correlation, and linear regression equations for the parameters outlined in the aim of the study were calculated. Results. Hyperbaric oxygen caused damages of erythrocytes at the 34th minute after beginning of the treatment. Various forms of abnormal red blood cells occured, and immediately before the occurrence of irreversible changes (erythrocyte membrane rupture) echinocyte shape was dominated. A significant correlation between the number of damaged red blood cells at 34th minute and their number at the 36th, 38th and 40th minute was found. Convulsions were diagnosed significantly earlier in mice with a greater number of damaged red blood cells (p < 0.01). There was a negative correlation between the number of irreversiblly damaged red blood cells (ruptured) at the 40th minute and neurological score in the studied animals (p < 0.05). Conclusion. The analysis of altered erythrocytes during hyperbaric oxygenation could predict a moment of seizures occurrence, and therefore the duration of the therapy with hyperbaric oxygen. Ehinocytes indicate impending rupture of red blood cells and a possible occurrence of seizures. An increased number of ruptured red blood cells may also even indicate the potential burden of CNS after cessation of hyperbaric oxygenation.

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