Athb-12, a homeobox-leucine zipper domain protein from Arabidopsis thaliana, increases salt tolerance in yeast by regulating sodium exclusion

Abstract In flowering plants, repression of the seed maturation program is essential for the transition from the seed to the vegetative phase, but the underlying mechanisms remain poorly understood. The B3-domain protein VIVIPAROUS1/ABSCISIC ACID-INSENSITIVE3-LIKE 1 (VAL1) is involved in repressing the seed maturation program. Here we uncovered a molecular network triggered by the plant hormone brassinosteroid (BR) that inhibits the seed maturation program during the seed-to-seedling transition in Arabidopsis (Arabidopsis thaliana). val1-2 mutant seedlings treated with a BR biosynthesis inhibitor form embryonic structures, whereas BR signaling gain-of-function mutations rescue the embryonic structure trait. Furthermore, the BR-activated transcription factors BRI1-EMS-SUPPRESSOR 1 and BRASSINAZOLE-RESISTANT 1 bind directly to the promoter of AGAMOUS-LIKE15 (AGL15), which encodes a transcription factor involved in activating the seed maturation program, and suppress its expression. Genetic analysis indicated that BR signaling is epistatic to AGL15 and represses the seed maturation program by downregulating AGL15. Finally, we showed that the BR-mediated pathway functions synergistically with the VAL1/2-mediated pathway to ensure the full repression of the seed maturation program. Together, our work uncovered a mechanism underlying the suppression of the seed maturation program, shedding light on how BR promotes seedling growth.

Download Full-text

Overexpression of β-Ketoacyl-CoA Synthase From Vitis vinifera L. Improves Salt Tolerance in Arabidopsis thaliana

Frontiers in Plant Science ◽

10.3389/fpls.2020.564385 ◽

2020 ◽

Vol 11 ◽

Author(s):

Zhen Yang ◽

Xue Yang ◽

Shujia Dong ◽

Yao Ge ◽

Xuenan Zhang ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Salt Tolerance ◽

Vitis Vinifera ◽

Vitis Vinifera L

Download Full-text

Correction for Kang et al., Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.0803745105 ◽

2008 ◽

Vol 105 (22) ◽

pp. 7893-7893

Keyword(s):

Arabidopsis Thaliana ◽

Salt Tolerance ◽

Golgi Apparatus ◽

Glycosylated Proteins

Download Full-text

Heterologous Overexpression of ZmHDZIV13 Enhanced Drought and Salt Tolerance in Arabidopsis and Tobacco

10.21203/rs.3.rs-697943/v1 ◽

2021 ◽

Author(s):

Fang Wang ◽

Peng Fang ◽

Huiping Yan ◽

Xiangzhuo Ji ◽

Yunling Peng

Keyword(s):

Transcription Factor ◽

Abiotic Stress ◽

Salt Tolerance ◽

Leucine Zipper ◽

Abiotic Stress Tolerance ◽

Heterologous Overexpression ◽

Malondialdehyde Content ◽

Drought And Salt Tolerance ◽

Relative Water ◽

Dependent Signaling Pathway

Abstract The homeodomain leucine zipper (HD-Zip) IV transcription factor is indispensable in the response of plants to abiotic stress. Systematic studies have been carried out in Arabidopsis, rice and other species from which a series of stress resistance-related genes have been isolated. However, the function of the HD-Zip-IV protein in maize is not clear. In this study, we cloned the HD-Zip-IV gene ZmHDZIV13 and identified its function in the stress response. Our phylogenetic analysis showed that ZmHDZIV13 and AtHDG11 had high homology and might have similar functions. The heterologous overexpression of ZmHDZIV13 in Arabidopsis resulted in sensitivity to abscisic acid (ABA), salt tolerance during germination and drought tolerance in seedlings. Under drought stress, the transgenic Arabidopsis showed stronger drought resistance than the wild-type showed (control). The malondialdehyde content of ZmHDZIV13 transgenic plants was lower than that of the control, and the relative water content and proline content were significantly higher than those of the control. After the drought was relieved, the expression of P5CS1, RD22, RD29B, RD29A, NCED3 and ERD1 were upregulated in transgenic Arabidopsis. Also, modified tobacco plants (35S::ZmHDZIV13) exhibited proper stomatal changes in response to drought conditions. These results show that ZmHDZIV13, as a stress-responsive transcription factor, plays a role in the positive regulation of abiotic stress tolerance and can regulate an ABA-dependent signaling pathway to regulate drought response in plants.

Download Full-text

Leucine Zipper Domain of HIV-1 gp41 Interacted Specifically with α-Catenin

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.2002.6583 ◽

2002 ◽

Vol 291 (5) ◽

pp. 1239-1244 ◽

Cited By ~ 8

Author(s):

Jong Tae Kim ◽

Eun Mi Kim ◽

Kyoung Hoa Lee ◽

Ji-Eun Choi ◽

Byung H. Jhun ◽

...

Keyword(s):

Leucine Zipper ◽

Leucine Zipper Domain ◽

Hiv 1

Download Full-text

Enhancement by lithium of cAMP-induced CRE/CREB-directed gene transcription conferred by TORC on the CREB basic leucine zipper domain

Biochemical Journal ◽

10.1042/bj20070796 ◽

2007 ◽

Vol 408 (1) ◽

pp. 69-77 ◽

Cited By ~ 24

Author(s):

Ulrike Böer ◽

Julia Eglins ◽

Doris Krause ◽

Susanne Schnell ◽

Christof Schöfl ◽

...

Keyword(s):

Dna Binding ◽

Gene Transcription ◽

Leucine Zipper ◽

Luciferase Reporter ◽

Lithium Salts ◽

Transactivation Domain ◽

Camp Response Element ◽

Basic Leucine Zipper ◽

Response Element ◽

Leucine Zipper Domain

The molecular mechanism of the action of lithium salts in the treatment of bipolar disorder is not well understood. As their therapeutic action requires chronic treatment, adaptive neuronal processes are suggested to be involved. The molecular basis of this are changes in gene expression regulated by transcription factors such as CREB (cAMP-response-element-binding protein). CREB contains a transactivation domain, in which Ser119 is phosphorylated upon activation, and a bZip (basic leucine zipper domain). The bZip is involved in CREB dimerization and DNA-binding, but also contributes to CREB transactivation by recruiting the coactivator TORC (transducer of regulated CREB). In the present study, the effect of lithium on CRE (cAMP response element)/CREB-directed gene transcription was investigated. Electrically excitable cells were transfected with CRE/CREB-driven luciferase reporter genes. LiCl (6 mM or higher) induced an up to 4.7-fold increase in 8-bromo-cAMP-stimulated CRE/CREB-directed transcription. This increase was not due to enhanced Ser119 phosphorylation or DNA-binding of CREB. Also, the known targets inositol monophosphatase and GSK3β (glycogen-synthase-kinase 3β) were not involved as specific GSK3β inhibitors and inositol replenishment did not mimic and abolish respectively the effect of lithium. However, lithium no longer enhanced CREB activity when the CREB-bZip was deleted or the TORC-binding site inside the CREB-bZip was specifically mutated (CREB-R300A). Otherwise, TORC overexpression conferred lithium responsiveness on CREB-bZip or the CRE-containing truncated rat somatostatin promoter. This indicates that lithium enhances cAMP-induced CRE/CREB-directed transcription, conferred by TORC on the CREB-bZip. We thus support the hypothesis that lithium salts modulate CRE/CREB-dependent gene transcription and suggest the CREB coactivator TORC as a new molecular target of lithium.

Download Full-text