N-terminus plus linker domain of Mg-chelatase D subunit is essential for Mg-chelatase activity in Oryza sativa

2018 ◽  
Vol 497 (2) ◽  
pp. 749-755 ◽  
Author(s):  
Sha Luo ◽  
Tao Luo ◽  
Yinan Liu ◽  
Zunwen Li ◽  
Shuying Fan ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
N Terminus ◽  
Linker Domain ◽  
Mg Chelatase ◽  
D Subunit

scholarly journals Scaffold subunits support associated subunit assembly in the Chlamydomonas ciliary nexin-dynein regulatory complex

2019 ◽  
Author(s):  
Long Gui ◽  
Kangkang Song ◽  
Douglas Tristchler ◽  
Raqual Bower ◽  
Yan Si ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Core Complex ◽  
Ciliary Motility ◽  
C Terminus ◽  
Large N ◽  
Cryo Electron Tomography ◽  
N Terminus ◽  
Regulatory Complex ◽  
Cilia And Flagella ◽  
Linker Domain

ABSTRACTThe nexin-dynein regulatory complex (N-DRC) in motile cilia and flagella functions as a linker between neighboring doublet microtubules, acts to stabilize the axonemal core structure, and serves as a central hub for the regulation of ciliary motility. Although the N-DRC has been studied extensively using genetic, biochemical, and structural approaches, the precise arrangement of the eleven (or more) N-DRC subunits remains unknown. Here, using cryo-electron tomography, we have compared the structure of Chlamydomonas wild-type flagella to that of strains with specific DRC subunit deletions or rescued strains with tagged DRC subunits. Our results show that DRC7 is a central linker subunit that helps connect the N-DRC to the outer dynein arms. DRC11 is required for the assembly of DRC8, and DRC8/11 form a sub-complex in the proximal lobe of the linker domain that is required to form stable contacts to the neighboring B-tubule. Gold labeling of tagged subunits determines the precise locations of the previously ambiguous N-terminus of DRC4 which is now shown to contribute to the core scaffold of the N-DRC and C-terminus of DRC5. Our results reveal the overall architecture of N-DRC, with the three subunits, DRC1/2/4 forming a core complex that serves as the scaffold for the assembly of the “functional subunits” associate, namely DRC3/5-8/11. These findings shed light on N-DRC assembly and its role in regulating flagellar beating.Significance StatementCilia and flagella are small hair-like appendages in eukaryotic cells that play essential roles in cell sensing, signaling, and motility. The highly conserved nexin-dynein regulatory complex (N-DRC) is one of the key regulators for ciliary motility. At least 11 proteins (DRC1–11) have been assigned to the N-DRC, but their precise arrangement within the large N-DRC structure is not yet known. Here, using cryo-electron tomography combined with genetic approaches, we have localized DRC7, the sub-complex DRC8/DRC11, the N-terminus of DRC4, and the C-terminus of DRC5. Our results provide insights into the N-DRC structure, its function in the regulation of dynein activity, and the mechanism by which n-drc mutations can lead to defects in ciliary motility that cause disease.

Reconstitution of barley photosystem I reveals that the N-terminus of the PSI-D subunit is essential for tight binding of PSI-C

1995 ◽  
Vol 95 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Helle Naver ◽  
M. Paul Scott ◽  
Birgitte Andersen ◽  
Birger Lindberg Moller ◽  
Henrik Vibe Scheller
Keyword(s):  
Photosystem I ◽  
Tight Binding ◽  
N Terminus ◽  
D Subunit

Reconstitution of barley photosystem I reveals that the N-terminus of the PSI-D subunit is essential for tight binding of PSI-C

1995 ◽  
Vol 95 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Helle Naver ◽  
M. Paul Scott ◽  
Birgitte Andersen ◽  
Birger Lindberg Moller ◽  
Henrik Vibe Scheller
Keyword(s):  
Photosystem I ◽  
Tight Binding ◽  
N Terminus ◽  
D Subunit

Characteristics of the Interaction between Thrombin Exosite1 and the Sequence 269-297 of Platelet Glycoprotein Ibα

1998 ◽  
Vol 80 (08) ◽  
pp. 310-315 ◽  
Author(s):  
Marie-Christine Bouton ◽  
Christophe Thurieau ◽  
Marie-Claude Guillin ◽  
Martine Jandrot-Perrus
Keyword(s):  
Platelet Activation ◽  
Electrostatic Interactions ◽  
Platelet Glycoprotein ◽  
Thrombin Receptor ◽  
Central Position ◽  
Amidolytic Activity ◽  
N Terminus ◽  
Hydrolysis Of ◽  
Chemical Cross Linking ◽  
Positively Charged

SummaryThe interaction between GPIb and thrombin promotes platelet activation elicited via the hydrolysis of the thrombin receptor and involves structures located on the segment 238-290 within the N-terminal domain of GPIbα and the positively charged exosite 1 on thrombin. We have investigated the ability of peptides derived from the 269-287 sequence of GPIbα to interact with thrombin. Three peptides were synthesized, including Ibα 269-287 and two scrambled peptides R1 and R2 which are comparable to Ibα 269-287 with regards to their content and distribution of anionic residues. However, R2 differs from both Ibα 269-287 and R1 by the shifting of one proline from a central position to the N-terminus. By chemical cross-linking, we observed the formation of a complex between 125I-Ibα 269-287 and α-thrombin that was inhibited by hirudin, the C-terminal peptide of hirudin, sodium pyrophosphate but not by heparin. The complex did not form when γ-thrombin was substituted for α-thrombin. Ibα 269-287 produced only slight changes in thrombin amidolytic activity and inhibited thrombin binding to fibrin. R1 and R2 also formed complexes with α-thrombin, modified slightly its catalytic activity and inhibited its binding to fibrin. Peptides Ibα 269-287 and R1 inhibited platelet aggregation and secretion induced by low thrombin concentrations whereas R2 was without effect. Our results indicate that Ibα 269-287 interacts with thrombin exosite 1 via mainly electrostatic interactions, which explains why the scrambled peptides also interact with exosite 1. Nevertheless, the lack of effect of R2 on thrombin-induced platelet activation suggests that proline 280 is important for thrombin interaction with GPIb.

Keragaan Morfologi dan Kandungan Antosianin Padi Beras Merah (Oryza sativa L.) di Kecamatan Munte dan Kecamatan Payung, Kabupaten Karo

1970 ◽  
Vol 5 (3) ◽  
pp. 391-403
Author(s):  
Wiwik Winarti ◽  
Eva Sartini Bayu ◽  
Revandy Iskandar Damanik
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
International Rice Research Institute ◽  
Research Institute

Keragaan morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.) pada Kecamatan Munte dan Kecamatan Payung di Kabupaten Karo. Penelitian ini bertujuan untuk mendapatkan informasi morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.). Penelitian ini dimulai dari Juni 2017dan selesai pada Januari 2018 di kecamatan Munte dan Kecamatan Payung Kabupaten Karo.Metode survei deskriptif menggunakan panduan International Rice Research Institute (IRRI). Teknik penentuan lokasi secara sengaja dan pengamblan sampel secara kebetulan.Uji kandungan antosianin menggunakan metode analitik dengan menghomogenkan sampel. Hasil eksplorasi didapatkan 72 genotipe yang dibagi menjadi tiga lokasi lahan. Berdasaran uji kandungan antosianin didapatkan lahan A memiliki kandungan tertinggi yaitu 0,5 mg/100 g dan terendah yaitu lahan B 0,08 mg/100 g.

Efficacy of some insecticide modules against major insect pests and spider population of rice, Oryza sativa L.

ENTOMON ◽  
2018 ◽  
Vol 43 (4) ◽  
pp. 257-262
Author(s):  
Atanu Seni ◽  
Bhimasen Naik
Keyword(s):  
Oryza Sativa ◽  
Grain Yield ◽  
Untreated Control ◽  
Insect Pests ◽  
Oryza Sativa L ◽  
Gall Midge ◽  
Stem Borer ◽  
The Other ◽  
The Third ◽  
Treated Plot

Experiments were carried out to assess some insecticide modules against major insect pests of rice. Each module consists of a basal application of carbofuran 3G @ 1 kg a.i ha-1 at 20 DAT and Rynaxypyr 20 SC @ 30 g a.i ha-1 at 45 DAT except untreated control. All modules differ with each other only in third treatment which was applied in 65 DAT. The third treatment includes: Imidacloprid 17.8 SL @ 27 g a.i ha-1, Pymetrozine 50 WG @ 150 g a.i ha-1, Triflumezopyrim 106 SC @ 27 g a.i ha-1, Buprofezin 25 SC @ 250 g a.i ha-1; Glamore (Imidacloprid 40+Ethiprole 40% w/w) 80 WG @ 100 g a.i. ha-1, Thiacloprid 24 SC @ 60 g a.i ha-1, Azadirachtin 0.03 EC @ 8 g a.i ha-1, Dinotefuran 20 SG@ 40 g a.i ha-1 and untreated control. All the treated plots recorded significantly lower percent of dead heart, white ear- head caused by stem borer and silver shoot caused by gall midge. Module with Pymetrozine 50 WG @ 150 g a.i ha-1 treated plot recorded significantly higher per cent reduction of plant hoppers (>80% over untreated control) and produced higher grain yield (50.75 qha-1) than the other modules. Among the different treated modules the maximum number of spiders was found in Azadirachtin 0.03 EC @ 8 g a.i ha-1 treated module plot followed by other treatments.

Correlation Studies on Grain Yield and its Component Traits in Rice (Oryza sativa L.)

2012 ◽  
Vol 2 (11) ◽  
pp. 13-14
Author(s):  
R. ARULMOZHI R. ARULMOZHI ◽  
◽  
Dr. A. MUTHUSWAMY Dr. A. MUTHUSWAMY
Keyword(s):  
Oryza Sativa ◽  
Grain Yield ◽  
Oryza Sativa L ◽  
Correlation Studies ◽  
Component Traits

Aplikasi Abu Sekam pada Padi Gogo (Oryza sativa L.) terhadap Kandungan Silikat dan Prolin Daun serta Amilosa dan Protein Biji

2011 ◽  
Vol 16 (1) ◽  
Author(s):  
Ahadiyat Yugi Rahayu ◽  
Tri Harjoso
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Screen House

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh aplikasi abu sekam terhadap kandungan prolin dan silikat daun serta kualitas hasil yaitu protein dan amilosa biji padi gogo dan hubungan korelasi antar keempat komponen tersebut pada kondisi pertanaman 80 persen kapasitas lapang pada skala pot. Penelitian dilakukan di polibag dalam screen house Fakultas Pertanian Unsoed dengan menggunakan rancangan acak kelompok pola faktorial dengan faktor varietas (Situ patenggang, Limboto, Towuti, Batutegi dan Aek sibundong) dan faktor abu sekam (0, 2, 4, 6 t/ha), diulang tiga kali. Hasil penelitian menunjukan bahwa pemberian abu sekam dosis 2−6 t/ha mampu meningkatkan kandungan silikat daun antara 18,49−29,43% dan menurunkan kandungan amilosa biji pada lima varietas sekitar 4,19−6,92%. Pemberian abu sekam dosis 2−6 t/ha mampu meningkatkan kandungan prolin daun antara 27,56−70,63% dan protein biji antara 2,35−16,71%. Antarvarietas menunjukan bahwa kandungan prolin tertinggi dihasilkan oleh varietas Batu tegi 18,58 persen dan protein biji pada varietas Situ patenggang 9,55%. Terdapat korelasi antar karakter fisiologis yaitu antara silikat-prolin (0,62) dan kandungan protein-amilosa biji (-0,78).

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