Functional remodeling after vocal fold injury by small intestinal submucosa gel containing hepatocyte growth factor

Biomaterials ◽  
2015 ◽  
Vol 40 ◽  
pp. 98-106 ◽  
Author(s):  
Jeong-Seok Choi ◽  
Songyi Lee ◽  
Da Yeon Kim ◽  
Young-Mo Kim ◽  
Moon Suk Kim ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Vocal Fold ◽  
Small Intestinal Submucosa ◽  
Functional Remodeling ◽  
Small Intestinal ◽  
Intestinal Submucosa ◽  
Hepatocyte Growth

scholarly journals Controlled release of hepatocyte growth factor from a bovine acellular scaffold for vocal fold reconstruction

2009 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
Chet C. Xu ◽  
Roger W. Chan ◽  
Debra G. Weinberger ◽  
Guy Efune ◽  
Karen S. Pawlowski
Keyword(s):  
Controlled Release ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Vocal Fold ◽  
Acellular Scaffold ◽  
Hepatocyte Growth

Effect of Exogenous Hepatocyte Growth Factor on Vocal Fold Fibroblasts

2009 ◽  
Vol 118 (8) ◽  
pp. 606-611 ◽  
Author(s):  
Yo Kishimoto ◽  
Shigeru Hirano ◽  
Atsushi Suehiro ◽  
Ichiro Tateya ◽  
Shin-Ichi Kanemaru ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Vocal Fold ◽  
Transforming Growth Factor ◽  
Therapeutic Potential ◽  
Vocal Folds ◽  
Messenger Rnas ◽  
Extracellular Matrix Components ◽  
Hepatocyte Growth ◽  
Tgf Β1

Objectives We have previously demonstrated the therapeutic potential of hepatocyte growth factor (HGF) in the treatment of vocal fold scarring, although how exogenous HGF affects gene expression of endogenous HGF or extracellular matrix components in the vocal fold fibroblasts remains unclear. In this in vitro study, we aimed to clarify this aspect in order to better understand the effects of HGF on the vocal folds. Methods Fibroblasts were obtained from the lamina propria of the vocal folds of 5 Sprague-Dawley rats and were cultured with HGF at concentrations of 100, 10, 1, and 0 ng/mL. The cells were collected on days 1, 3, and 7, and the expression of endogenous HGF, its receptor c-Met, transforming growth factor-β1 (TGF-β1), procollagen types I and III, and hyaluronic acid synthase (HAS)-l and HAS-2 messenger RNAs (mRNAs) was examined by real-time reverse transcription-polymerase chain reaction. Results The expression of endogenous HGF and HAS-1 mRNAs increased significantly when exogenous HGF was administered at a concentration of 1 ng/mL. On day 1, the expression of TGF-β1 and HAS-2 mRNAs increased significantly in response to 1 ng/mL HGF. Conclusions Exogenous HGF triggered the up-regulation of endogenous HGF, TGF-β1, HAS-1, and HAS-2 mRNAs in vocal fold fibroblasts.

Controlled release of hepatocyte growth factor from a bovine acellular scaffoldfor vocal fold reconstruction

2009 ◽  
Vol 119 (S3) ◽  
pp. S277-S277
Author(s):  
Chet C. Xu ◽  
Roger W. Chan ◽  
Debra G. Weinberger ◽  
Guy Efune ◽  
Karen S. Pawlowski
Keyword(s):  
Controlled Release ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Vocal Fold ◽  
Hepatocyte Growth

Hepatocyte Growth Factor and its Receptor C-Met in Rat and Rabbit Vocal Folds

2002 ◽  
Vol 111 (8) ◽  
pp. 661-666 ◽  
Author(s):  
Shigeru Hirano ◽  
Susan Thibeault ◽  
Charles N. Ford ◽  
Diane M. Bless ◽  
Shin-Ichi Kanemaru
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Lamina Propria ◽  
Vocal Fold ◽  
Vocal Folds ◽  
Enzyme Linked Immunosorbent Assay ◽  
Alternative Approach ◽  
Fibrotic Scar ◽  
Hepatocyte Growth

Vocal fold fibrotic scar is characterized by fibrosis of the lamina propria and epithelium, and is difficult to treat. Hepatocyte growth factor (HGF) has antifibrotic activity and has received attention as a possible therapeutic alternative to treat fibrosis. In this study, in order to clarify whether HGF can be involved in vocal fold scarring, we examined the existence of HGF and its receptor, c-Met, in rat vocal folds, and then the activity of HGF in rabbit injured vocal folds, using immunohistochemistry and enzyme-linked immunosorbent assay. We found HGF and c-Met on epithelial cells and gland cells of the rat vocal folds. On the injured vocal folds of rabbits, little HGF was observed immediately after injury, but prominent activity occurred simultaneously with reepithelialization of the vocal fold mucosa on days 10 to 15. The activity of HGF was observed on fibroblasts in the lamina propria, as well as the epithelium. It is suggested that HGF in the vocal folds is produced by the fibroblasts and delivered to the epithelium. The implication of these findings is that HGF is involved in wound healing of the vocal fold, and may provide an alternative approach in preventing and treating vocal fold scarring.

Controlled Release of Hepatocyte Growth Factor from MPEG-b-(PCL-ran-PLLA) Diblock Copolymer for Improved Vocal Fold Regeneration

2016 ◽  
Vol 17 (2) ◽  
pp. 1600163 ◽  
Author(s):  
Jae Won Choi ◽  
Yeon Soo Kim ◽  
Ju Kyeong Park ◽  
Eun Hye Song ◽  
Ji Hoon Park ◽  
...  
Keyword(s):  
Controlled Release ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Diblock Copolymer ◽  
Vocal Fold ◽  
Hepatocyte Growth

Efficacy of autologous fat injection laryngoplasty with an adenoviral vector expressing hepatocyte growth factor in a canine model

2009 ◽  
Vol 123 (S31) ◽  
pp. 24-29 ◽  
Author(s):  
H Umeno ◽  
S Chitose ◽  
Y Murofushi ◽  
K Kosai ◽  
K Sato ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Adenoviral Vector ◽  
Vocal Fold ◽  
Injection Laryngoplasty ◽  
Fat Tissue ◽  
Fat Injection ◽  
Autologous Fat ◽  
Hepatocyte Growth

AbstractObjective:The effectiveness of autologous fat injection laryngoplasty may be reduced by resorption of injected fat tissue. The aim of the present study was to clarify the efficacy of fat injection laryngoplasty using autologous fat plus a replication-defective adenoviral vector expressing hepatocyte growth factor, regarding reduction of injected fat tissue resorption.Material and methods:Four female beagle dogs were used in this study. After sedation, a direct laryngoscope was introduced to enable visualisation of the larynx. In each dog, harvested autologous fat plus an adenoviral vector expressing hepatocyte growth factor was injected into the right true vocal fold, and harvested fat plus an adenoviral vector expressing no gene was injected into the left true vocal fold. A total laryngectomy was performed one year after the intracordal fat injection. Coronal sections of the resected whole larynges were made and the following parameters assessed using light and electron microscopy: size of fat area; number of vasculoendothelial cells surrounding adipocytes; and shape of injected adipocytes in the vocal fold.Results:The fat area was significantly larger and the number of vasculoendothelial cells surrounding adipocytes significantly greater in the intracordal fat injection containing adenoviral vector expressing hepatocyte growth factor, compared with the control intracordal fat injection containing adenoviral vector expressing no gene. When viewed under electron microscopy, the injected adipocytes were observed to have grafted better in the intracordal fat injection with hepatocyte growth factor adenoviral vector, compared with the control intracordal fat injection with adenoviral vector expressing no gene.Conclusions:Injection into the vocal fold of autologous fat containing an adenoviral vector expressing hepatocyte growth factor can reduce subsequent resorption of injected fat.

Hepatocyte growth factor stimulates invasion across reconstituted basement membranes by a new human small intestinal cell line

1994 ◽  
Vol 12 (2) ◽  
pp. 143-154 ◽  
Author(s):  
Iruvanti Sunitha ◽  
Deborah L. Meighen ◽  
Dan -Paul Hartman ◽  
Erik W. Thompson ◽  
Stephen W. Byers ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Cell Line ◽  
Basement Membranes ◽  
Intestinal Cell ◽  
Intestinal Cell Line ◽  
Small Intestinal ◽  
Hepatocyte Growth
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