Isopropanol-butanol production from sugarcane and sugarcane-sweet sorghum juices by Clostridium beijerinckii DSM 6423

Biobutanol can be produced by Clostridia via an acetone–butanol–ethanol (ABE) fermentation under strictly anaerobic conditions. Oxygen-free nitrogen (OFN) gas is typically used to create anaerobic conditions for ABE fermentations. However, this method is not appropriate for large-scale fermentations as it is quite costly. The aim of this work was to study the feasibility of butanol production from sweet sorghum juice (SSJ) by Clostridium beijerinckii TISTR 1461 using various methods to create anaerobic conditions, i.e., growth of a strictly aerobic bacterium, an Arthrobacter sp., under different conditions and a chemical method using sodium dithionite (SDTN) to consume residual oxygen. SSJ containing 60 g/L of total sugar supplemented with 1.27 g/L of (NH4)2SO4 was used as a substrate for butanol production. The results showed that 0.25 mM SDTN could create anaerobic conditions, but in this case, C.beijerinckii TISTR 1461 could produce butanol at a concentration (PB) of only 8.51 g/L with a butanol productivity (QB) of 0.10 g/L·h. Arthrobacter sp. BCC 72131 could also be used to create anaerobic conditions. Mixed cultures of C.beijerinckii TISTR 1461 and Arthrobacter sp. BCC 72131 created anaerobic conditions by inoculating the C.beijerinckii 4 h after Arthrobacter. This gave a PB of 10.39 g/L with a QB of 0.20 g/L·h. Comparing butanol production with the control treatment (using OFN gas to create anaerobic conditions, yielding a PB of 9.88 g/L and QB of 0.21 g/L·h) indicated that using Arthrobacter sp. BCC 72131 was an appropriate procedure for creating anaerobic conditions for high levels of butanol production by C. beijerinckii TISTR 1461 from a SSJ medium.

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Optimization of nutrient supplements for butanol production from sweet sorghum stem juice by Clostridium beijerinckii using an orthogonal array design

Current Opinion in Biotechnology ◽

10.1016/j.copbio.2013.05.442 ◽

2013 ◽

Vol 24 ◽

pp. S137

Author(s):

Likit Sirisantimethakom ◽

Patthranit Sanchanda ◽

Jetnipit Chatleudmongkol ◽

Lakkana Laopaiboon ◽

Pattana Laopaiboon

Keyword(s):

Orthogonal Array ◽

Sweet Sorghum ◽

Clostridium Beijerinckii ◽

Butanol Production ◽

Orthogonal Array Design ◽

Array Design ◽

Nutrient Supplements

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Butanol production from wheat straw by simultaneous saccharification and fermentation using Clostridium beijerinckii: Part II—Fed-batch fermentation

Biomass and Bioenergy ◽

10.1016/j.biombioe.2007.07.005 ◽

2008 ◽

Vol 32 (2) ◽

pp. 176-183 ◽

Cited By ~ 76

Author(s):

Nasib Qureshi ◽

Badal C. Saha ◽

Michael A. Cotta

Keyword(s):

Wheat Straw ◽

Batch Fermentation ◽

Simultaneous Saccharification And Fermentation ◽

Clostridium Beijerinckii ◽

Butanol Production ◽

Fed Batch ◽

Fed Batch Fermentation ◽

Simultaneous Saccharification

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Comparison of different Vetiver grass pretreatment techniques and their impact on immobilized butanol production by Clostridium beijerinckii TISTR 1461

Cellulose ◽

10.1007/s10570-021-04101-4 ◽

2021 ◽

Author(s):

Fuangfa Srisuk ◽

Piyawat Chinwatpaiboon ◽

Thanaphat Atjayutpokin ◽

Akarin Boonsombuti ◽

Ancharida Savarajara ◽

...

Keyword(s):

Clostridium Beijerinckii ◽

Vetiver Grass ◽

Butanol Production

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Developing a coculture for enhanced butanol production by Clostridium beijerinckii and Saccharomyces cerevisiae

Bioresource Technology Reports ◽

10.1016/j.biteb.2019.03.006 ◽

2019 ◽

Vol 6 ◽

pp. 223-228 ◽

Cited By ~ 10

Author(s):

Jiwen Wu ◽

Lili Dong ◽

Chunshuang Zhou ◽

Bingfeng Liu ◽

Liping Feng ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Clostridium Beijerinckii ◽

Butanol Production

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Global View of Biofuel Butanol and Economics of Its Production by Fermentation from Sweet Sorghum Bagasse, Food Waste, and Yellow Top Presscake: Application of Novel Technologies

Fermentation ◽

10.3390/fermentation6020058 ◽

2020 ◽

Vol 6 (2) ◽

pp. 58

Author(s):

N. Qureshi ◽

X. Lin ◽

S. Liu ◽

B. C. Saha ◽

A. P. Mariano ◽

...

Keyword(s):

Food Waste ◽

Sweet Sorghum ◽

The United States ◽

Product Recovery ◽

Selling Price ◽

Sweet Sorghum Bagasse ◽

Capital Investments ◽

Butanol Production ◽

Total Capital ◽

Sorghum Bagasse

Worldwide, there are various feedstocks such as straws, corn stover, sugarcane bagasse, sweet sorghum bagasse (SSB), grasses, leaves, whey permeate, household organic waste, and food waste (FW) that can be converted to valuable biofuels such as butanol. For the present studies, an economic analysis was performed to compare butanol production from three feedstocks (SSB; FW; and yellow top presscake, YTP or YT) using a standard process and an advanced integrated process design. The total plant capacity was set at 170,000–171,000 metric tons of total acetone butanol ethanol (ABE) per year (99,300 tons of just butanol per year). Butanol production from SSB typically requires pretreatment, separate hydrolysis, fermentation, and product recovery (SHFR). An advanced process was developed in which the last three steps were combined into a single unit operation for simultaneous saccharification, fermentation, and recovery (SSFR). For the SHFR and SSFR plants, the total capital investments were estimated as $213.72 × 106 and $198.16 × 106, respectively. It was further estimated that the minimum butanol selling price (using SSB as a feedstock) for the two processes were $1.14/kg and $1.05/kg. Therefore, SSFR lowered the production cost markedly compared to that of the base case. Butanol made using FW had an estimated minimum selling price of only $0.42/kg. This low selling price is because the FW to butanol process does not require pretreatment, hydrolysis, and cellulolytic enzymes. For this plant, the total capital investment was projected to be $107.26 × 106. The butanol selling price using YTP as a feedstock was at $0.73/kg and $0.79/kg with total capital investments for SSFR and SHFR of $122.58 × 106 and $132.21 × 106, respectively. In the Results and Discussion section, the availability of different feedstocks in various countries such as Brazil, the European Union, New Zealand, Denmark, and the United States are discussed. Additionally, the use of various microbial strains and product recovery technologies are also discussed.

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Milling byproducts are an economically viable substrate for butanol production using clostridial ABE fermentation

Applied Microbiology and Biotechnology ◽

10.1007/s00253-020-10882-8 ◽

2020 ◽

Vol 104 (20) ◽

pp. 8679-8689

Author(s):

Nils Thieme ◽

Johanna C. Panitz ◽

Claudia Held ◽

Birgit Lewandowski ◽

Wolfgang H. Schwarz ◽

...

Keyword(s):

Clostridium Beijerinckii ◽

Abe Fermentation ◽

Liquid Fuels ◽

Butanol Production ◽

Enzymatic Pretreatment ◽

Profitability Analysis ◽

Key Points ◽

Wide Range ◽

Wheat Middlings ◽

Acetone Butanol Ethanol

Abstract Butanol is a platform chemical that is utilized in a wide range of industrial products and is considered a suitable replacement or additive to liquid fuels. So far, it is mainly produced through petrochemical routes. Alternative production routes, for example through biorefinery, are under investigation but are currently not at a market competitive level. Possible alternatives, such as acetone-butanol-ethanol (ABE) fermentation by solventogenic clostridia are not market-ready to this day either, because of their low butanol titer and the high costs of feedstocks. Here, we analyzed wheat middlings and wheat red dog, two wheat milling byproducts available in large quantities, as substrates for clostridial ABE fermentation. We could identify ten strains that exhibited good butanol yields on wheat red dog. Two of the best ABE producing strains, Clostridium beijerinckii NCIMB 8052 and Clostridium diolis DSM 15410, were used to optimize a laboratory-scale fermentation process. In addition, enzymatic pretreatment of both milling byproducts significantly enhanced ABE production rates of the strains C. beijerinckii NCIMB 8052 and C. diolis DSM 15410. Finally, a profitability analysis was performed for small- to mid-scale ABE fermentation plants that utilize enzymatically pretreated wheat red dog as substrate. The estimations show that such a plant could be commercially successful. Key points • Wheat milling byproducts are suitable substrates for clostridial ABE fermentation. • Enzymatic pretreatment of wheat red dog and middlings increases ABE yield. • ABE fermentation plants using wheat red dog as substrate are economically viable.

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