scholarly journals Neural crest-derived cells possess differentiation potential to keratinocytes in the process of wound healing

2022 ◽  
Vol 146 ◽  
pp. 112593
Author(s):  
Hideomi Takizawa ◽  
Akiko Karakawa ◽  
Tetsuo Suzawa ◽  
Masahiro Chatani ◽  
Megumi Ikeda ◽  
...  
Keyword(s):  
Wound Healing ◽  
Neural Crest ◽  
Differentiation Potential

scholarly journals Dynamic expression of MMP28 during cranial morphogenesis

2020 ◽  
Vol 375 (1809) ◽  
pp. 20190559 ◽  
Author(s):  
Nadege Gouignard ◽  
Eric Theveneau ◽  
Jean-Pierre Saint-Jeannet
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Neural Crest ◽  
Large Family ◽  
Tail Bud ◽  
Dynamic Expression ◽  
The Family ◽  
Extracellular Matrix Remodelling ◽  
And Migration ◽  
Human And Mouse

Matrix metalloproteinases (MMPs) are a large family of proteases comprising 24 members in vertebrates. They are well known for their extracellular matrix remodelling activity. MMP28 is the latest member of the family to be discovered. It is a secreted MMP involved in wound healing, immune system maturation, cell survival and migration. MMP28 is also expressed during embryogenesis in human and mouse. Here, we describe the detailed expression profile of MMP28 in Xenopus laevis embryos. We show that MMP28 is expressed maternally and accumulates at neurula and tail bud stages specifically in the cranial placode territories adjacent to migrating neural crest cells. As a secreted MMP, MMP28 may be required in neural crest–placode interactions. This article is part of a discussion meeting issue ‘Contemporary morphogenesis’.

scholarly journals Reactivation of the pluripotency program precedes formation of the cranial neural crest

Science ◽  
2021 ◽  
Vol 371 (6529) ◽  
pp. eabb4776 ◽  
Author(s):  
Antoine Zalc ◽  
Rahul Sinha ◽  
Gunsagar S. Gulati ◽  
Daniel J. Wesche ◽  
Patrycja Daszczuk ◽  
...  
Keyword(s):  
Neural Crest ◽  
Craniofacial Development ◽  
Open Chromatin ◽  
Cranial Neural Crest ◽  
Craniofacial Skeleton ◽  
Differentiation Potential ◽  
Subsequent Formation ◽  
Developmental Potential ◽  
Epiblast Stem Cells ◽  
Cranial Neural Crest Cells

During development, cells progress from a pluripotent state to a more restricted fate within a particular germ layer. However, cranial neural crest cells (CNCCs), a transient cell population that generates most of the craniofacial skeleton, have much broader differentiation potential than their ectodermal lineage of origin. Here, we identify a neuroepithelial precursor population characterized by expression of canonical pluripotency transcription factors that gives rise to CNCCs and is essential for craniofacial development. Pluripotency factor Oct4 is transiently reactivated in CNCCs and is required for the subsequent formation of ectomesenchyme. Furthermore, open chromatin landscapes of Oct4+ CNCC precursors resemble those of epiblast stem cells, with additional features suggestive of priming for mesenchymal programs. We propose that CNCCs expand their developmental potential through a transient reacquisition of molecular signatures of pluripotency.

scholarly journals Isolation and Characterization of a Human Fetal Mesenchymal Stem Cell Population: Exploring the Potential for Cell Banking in Wound Healing Therapies

2019 ◽  
Vol 28 (11) ◽  
pp. 1404-1419
Author(s):  
Roger Esteban-Vives ◽  
Jenny Ziembicki ◽  
Myung Sun Choi ◽  
R. L. Thompson ◽  
Eva Schmelzer ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Dermal Fibroblasts ◽  
Stem Cell Population ◽  
Differentiation Potential ◽  
Isolation And Characterization ◽  
Cell Banking ◽  
Lineage Stability

Various cell-based therapies are in development to address chronic and acute skin wound healing, for example for burns and trauma patients. An off-the-shelf source of allogeneic dermal cells could be beneficial for innovative therapies accelerating the healing in extensive wounds where the availability of a patient’s own cells is limited. Human fetal-derived dermal fibroblasts (hFDFs) show high in vitro division rates, exhibit low immunological rejection properties, and present scarless wound healing in the fetus, and previous studies on human fetal tissue-derived cell therapies have shown promising results on tissue repair. However, little is known about cell lineage stability and cell differentiation during the cell expansion process, required for any potential therapeutic use. We describe an isolation method, characterize a population, and investigate its potential for cell banking and thus suitability as a potential product for cell grafting therapies. Our results show hFDFs and a bone marrow-derived mesenchymal stem cell (BM-MSC) line shared identification markers and in vitro multilineage differentiation potential into osteogenic, chondrogenic, and adipogenic lineages. The hFDF population exhibited similar cell characteristics as BM-MSCs while producing lower pro-inflammatory cytokine IL-6 levels and higher levels of the wound healing factor hepatocyte growth factor. We demonstrate in vitro differentiation of hFDFs, which may be a problem in maintaining long-term lineage stability, potentially limiting their use for cell banking and therapy development.

scholarly journals Stem Cell-Based Therapeutics to Improve Wound Healing

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Michael S. Hu ◽  
Tripp Leavitt ◽  
Samir Malhotra ◽  
Dominik Duscher ◽  
Michael S. Pollhammer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Stem Cell ◽  
Wound Care ◽  
Clinical Applications ◽  
Wound Management ◽  
Tissue Formation ◽  
Wound Therapy ◽  
Differentiation Potential ◽  
Pathological Scar

Issues surrounding wound healing have garnered deep scientific interest as well as booming financial markets invested in novel wound therapies. Much progress has been made in the field, but it is unsurprising to find that recent successes reveal new challenges to be addressed. With regard to wound healing, large tissue deficits, recalcitrant wounds, and pathological scar formation remain but a few of our most pressing challenges. Stem cell-based therapies have been heralded as a promising means by which to surpass current limitations in wound management. The wide differentiation potential of stem cells allows for the possibility of restoring lost or damaged tissue, while their ability to immunomodulate the wound bed from afar suggests that their clinical applications need not be restricted to direct tissue formation. The clinical utility of stem cells has been demonstrated across dozens of clinical trials in chronic wound therapy, but there is hope that other aspects of wound care will inherit similar benefit. Scientific inquiry into stem cell-based wound therapy abounds in research labs around the world. While their clinical applications remain in their infancy, the heavy investment in their potential makes it a worthwhile subject to review for plastic surgeons, in terms of both their current and future applications.

scholarly journals Encapsulation of Adipose-Derived Mesenchymal Stem Cells in Calcium Alginate Maintains Clonogenicity and Enhances their Secretory Profile

2020 ◽  
Vol 21 (17) ◽  
pp. 6316
Author(s):  
Lucille Capin ◽  
Nacira Abbassi ◽  
Maëlle Lachat ◽  
Marie Calteau ◽  
Cynthia Barratier ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Calcium Alginate ◽  
Healing Process ◽  
Spherical Particles ◽  
Differentiation Potential ◽  
Monolayer Cultures ◽  
The Impact

Adipose-derived mesenchymal stem cells (ASCs) are well known for their secretory potential, which confers them useful properties in cell therapy. Nevertheless, this therapeutic potential is reduced after transplantation due to their short survival in the human body and their migration property. This study proposes a method to protect cells during and after injection by encapsulation in microparticles of calcium alginate. Besides, the consequences of encapsulation on ASC proliferation, pluripotential, and secretome were studied. Spherical particles with a mean diameter of 500 µm could be obtained in a reproducible manner with a viability of 70% after 16 days in vitro. Moreover, encapsulation did not alter the proliferative properties of ASCs upon return to culture nor their differentiation potential in adipocytes, chondrocytes, and osteocytes. Concerning their secretome, encapsulated ASCs consistently produced greater amounts of interleukin-6 (IL-6), interleukin-8 (IL-8), and vascular endothelial growth factor (VEGF) compared to monolayer cultures. Encapsulation therefore appears to enrich the secretome with transforming growth factor β1 (TGF-β1) and macrophage inflammatory protein-1β (MIP-1β) not detectable in monolayer cultures. Alginate microparticles seem sufficiently porous to allow diffusion of the cytokines of interest. With all these cytokines playing an important role in wound healing, it appears relevant to investigate the impact of using encapsulated ASCs on the wound healing process.

Localization and osteoblastic differentiation potential of neural crest-derived cells in oral tissues of adult mice

2015 ◽  
Vol 464 (4) ◽  
pp. 1209-1214 ◽  
Author(s):  
Miki Ono ◽  
Tetsuo Suzawa ◽  
Masamichi Takami ◽  
Gou Yamamoto ◽  
Tomohiko Hosono ◽  
...  
Keyword(s):  
Neural Crest ◽  
Osteoblastic Differentiation ◽  
Differentiation Potential ◽  
Adult Mice

scholarly journals Dynamic expression of MMP28 during cranial morphogenesis

2019 ◽  
Author(s):  
Nadege Gouignard ◽  
Eric Theveneau ◽  
Jean-Pierre Saint-Jeannet
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Immune System ◽  
Neural Crest ◽  
Large Family ◽  
Dynamic Expression ◽  
The Family ◽  
Extracellular Matrix Remodelling ◽  
And Migration ◽  
Human And Mouse

AbstractMatrix metalloproteinases (MMP) are a large family of proteases comprising 24 members in vertebrates. They are well known for their extracellular matrix remodelling activity. MMP28 is the last member of the family to be discovered. It is a secreted MMP involved in wound healing, immune system maturation, cell survival and migration. MMP28 is also expressed during embryogenesis in human and mouse. Here we describe the detailed expression profile of MMP28 in Xenopus laevis embryos. We show that MMP28 is expressed maternally and accumulates at neurula and tailbud stages specifically in the cranial placode territories adjacent to migrating neural crest cells. As a secreted MMP, MMP28 may be required in normal neural crest-placode interactions.

Sign in / Sign up

Export Citation Format

Share Document