Bioconversion of novel substrate Saccharum spontaneum, a weedy material, into ethanol by Pichia stipitis NCIM3498

2011 ◽  
Vol 102 (2) ◽  
pp. 1709-1714 ◽  
Author(s):  
Anuj K. Chandel ◽  
Om V. Singh ◽  
L. Venkateswar Rao ◽  
G. Chandrasekhar ◽  
M. Lakshmi Narasu
Keyword(s):  
Pichia Stipitis ◽  
Saccharum Spontaneum

DNA Barcoding and Phylogenetic Analysis Sugarcane (Saccharum officinarum L.) Based on matK ( maturase K) Gene

2020 ◽  
Vol 840 ◽  
pp. 162-170
Author(s):  
Ganies Riza Aristya ◽  
Fauzana Putri ◽  
Rina Sri Kasiamdari ◽  
Arni Musthofa
Keyword(s):  
Phylogenetic Tree ◽  
Molecular Level ◽  
Economic Value ◽  
Saccharum Officinarum ◽  
Saccharum Spontaneum ◽  
Dna Barcodes ◽  
Pcr Method ◽  
Hybrid Cultivar ◽  
I Gene ◽  
Sequencing Process

Sugarcane (Saccharum officinarum L.) is an agricultural commodities with a great extent of diversity and high economic value. In Indonesia, the great extent of diversity of sugarcane is evidenced by a large number of cultivars cultivated. Sugarcane diversities at the molecular level can be seen using DNA barcodes, one of which is the matK. The purpose of the study was to identify and characterize matK and reconstruct the phylogenetic tree to determine the phylogeny of 24 sugarcane cultivars Indonesia. matK was amplified using the PCR method with matK F-5’ATGATTAATTAAGAGTAAGAGGAT-3’ and matK R-5’AATGCAAAAATTCGAAGGGT-3. Results showed that the matK gene was successfully amplified as many as 1531 bp. The sequencing process was done to determine the nucleotide sequence and compared with those of the GenBank database. It showed that the samples used had a similarity of 98.87%-99.44% to that of matK in Saccharum officinarum, Saccharum hybrid cultivar and Saccharum spontaneum. Reconstruction of the phylogenetic tree showed that the samples used were located in the same clade with a zero genetic distance, while all the references from NCBI were also located in the same clade. The analysis of genetic variation indicated that it had no haplotype value.

Structural and mechanistic insight into stem‐loop RNA processing by yeast Pichia stipitis Dicer

2021 ◽  
Author(s):  
Chan Jing Ru ◽  
Fu Qinqin ◽  
Li Jianwei ◽  
Chen Ying ◽  
Satoru Machida ◽  
...  
Keyword(s):  
Rna Processing ◽  
Pichia Stipitis ◽  
Stem Loop ◽  
Mechanistic Insight ◽  
Insight Into

Ethanol Production from Corn Stover Using Soaking Pretreatment

2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Liquid Ratio ◽  
Mild Conditions ◽  
Whole Process ◽  
Conversion Rates ◽  
Mild Temperature ◽  
Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

scholarly journals 2-Deoxyglucose as a Selective Agent for Derepressed Mutants of Pichia stipitis

1999 ◽  
Vol 77 (1-3) ◽  
pp. 211-222 ◽  
Author(s):  
Hassan K. Sreenath ◽  
Thomas W. Jeffries
Keyword(s):  
Pichia Stipitis ◽  
Selective Agent
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