2-Deoxyglucose as a Selective Agent for Derepressed Mutants of Pichia stipitis

AbstractEndophytic fungi with the ability to produce plant based secondary metabolites are a potential alternative for producing the host plant metabolite and to prevent natural plants from extinction. To isolate a high metabolite yielding endophytic strain from plants, hundreds of endophytic strains are screened and tested for product yield separately under axenic state, before shortlisting the potential endophyte, which involves huge time consumption. In this study, strategies for screening and selection of high camptothecin yielding endophytes from their natural habitat were proposed. A correlation was built between the camptothecin yield in the explants and the endophytes isolated from them. In addition, camptothecin yield was compared between the endophytes isolated from young and matured plants. Further, camptothecin producers and non-producers strains were compared for their tolerance toward camptothecin. The study indicates that high camptothecin yielding endophytes were isolated from high yielding explants and younger plants and they were more tolerant to camptothecin in comparison to non-camptothecin yielding endophytes. Thus, choosing a young and high yielding explant for endophyte isolation, and use of camptothecin as a selective agent in the growth medium, can be instrumental in screening and selection of high camptothecin yielding endophytes from nature in relatively less time.

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Ethanol Production from Corn Stover Using Soaking Pretreatment

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.171-172.261 ◽

2010 ◽

Vol 171-172 ◽

pp. 261-265

Author(s):

Zhuang Zuo ◽

Xiu Shan Yang

Keyword(s):

Ethanol Production ◽

Corn Stover ◽

Simultaneous Saccharification And Fermentation ◽

Pichia Stipitis ◽

Liquid Ratio ◽

Mild Conditions ◽

Whole Process ◽

Conversion Rates ◽

Mild Temperature ◽

Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% deligniﬁcation, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous sacchariﬁcation and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

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Use of paromomycin as a selective agent for oat transformation

Plant Cell Reports ◽

10.1007/bf00232728 ◽

1995 ◽

Vol 14 (10) ◽

pp. 635-640 ◽

Cited By ~ 43

Author(s):

Kimberly A. Torbert ◽

Howard W. Rines ◽

David A. Somers

Keyword(s):

Selective Agent

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U50,488: A kappa-selective agent with poor affinity for mu1 opiate binding sites

Neuropharmacology ◽

10.1016/0028-3908(88)90052-4 ◽

1988 ◽

Vol 27 (3) ◽

pp. 331-332 ◽

Cited By ~ 35

Author(s):

Janet A. Clark ◽

G.W. Pasternak

Keyword(s):

Binding Sites ◽

Selective Agent ◽

Opiate Binding

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Ethanol production by co-fermentation of hexose and pentose from food wastes using Saccharomyces coreanus and Pichia stipitis

Korean Journal of Chemical Engineering ◽

10.1007/s11814-011-0282-3 ◽

2012 ◽

Vol 29 (8) ◽

pp. 1038-1043 ◽

Cited By ~ 19

Author(s):

Seung-Mi Jeong ◽

Yong-Jin Kim ◽

Dong-Hoon Lee

Keyword(s):

Ethanol Production ◽

Pichia Stipitis ◽

Food Wastes

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Study of Red Halophilic Bacteria in Solar Salt and Salted Fish: II. Bacto-oxgall as a Selective Agent for Differentiation

Journal of the Fisheries Research Board of Canada ◽

10.1139/f56-011 ◽

1956 ◽

Vol 13 (2) ◽

pp. 195-199 ◽

Cited By ~ 11

Author(s):

H. P. Dussault

Keyword(s):

Halophilic Bacteria ◽

Partial Identification ◽

Selective Medium ◽

Selective Agent ◽

Simple Method ◽

Solar Salt ◽

Salted Fish ◽

Low Concentrations ◽

Fish Samples

A simple method, based on oxgall tolerance, is proposed for differentiating red halophilic bacteria commonly found in solar salt and discoloured salted codfish. Tests carried out on 18 strains isolated from various sources have shown that the rod forms are inhibited by low concentrations of Bacto-oxgall and that the coccus forms tolerate relatively high ones. Bacto-oxgall can thus be used as the basis of a simple selective medium. This test has been found useful for the isolation, purification and partial identification of unidentified strains and also for determining the relative proportions of the two main types of red halophilic bacteria present in solar salt and salted fish samples.

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