Heterologous expression of Lactobacillus casei RecO improved the multiple-stress tolerance and lactic acid production in Lactococcus lactis NZ9000 during salt stress

2013 ◽  
Vol 143 ◽  
pp. 238-241 ◽  
Author(s):  
Chongde Wu ◽  
Juan Zhang ◽  
Guocheng Du ◽  
Jian Chen
Keyword(s):  
Lactic Acid ◽  
Salt Stress ◽  
Heterologous Expression ◽  
Stress Tolerance ◽  
Lactococcus Lactis ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Lactic Acid Production ◽  
Multiple Stress ◽  
Multiple Stress Tolerance

scholarly journals Expression of PprI from Deinococcus radiodurans Improves Lactic Acid Production and Stress Tolerance in Lactococcus lactis

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142918 ◽  
Author(s):  
Xiangrong Dong ◽  
Bing Tian ◽  
Shang Dai ◽  
Tao Li ◽  
Linna Guo ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Stress Tolerance ◽  
Lactococcus Lactis ◽  
Acid Production ◽  
Deinococcus Radiodurans ◽  
Lactic Acid Production

scholarly journals Improvement of Multiple-Stress Tolerance and Lactic Acid Production in Lactococcus lactis NZ9000 under Conditions of Thermal Stress by Heterologous Expression of Escherichia coli dnaK

2010 ◽  
Vol 76 (13) ◽  
pp. 4277-4285 ◽  
Author(s):  
Abdullah-Al-Mahin ◽  
Shinya Sugimoto ◽  
Chihana Higashi ◽  
Shunsuke Matsumoto ◽  
Kenji Sonomoto
Keyword(s):  
Escherichia Coli ◽  
Heat Treatment ◽  
Lactic Acid ◽  
Heterologous Expression ◽  
Stress Tolerance ◽  
Lactococcus Lactis ◽  
Lactic Acid Production ◽  
Expression System ◽  
Lactic Acid Fermentation ◽  
Acid Fermentation

ABSTRACT The effects of nisin-induced dnaK expression in Lactococcus lactis were examined, and this expression was shown to improve stress tolerance and lactic acid fermentation efficiency. Using a nisin-inducible expression system, DnaK proteins from L. lactis (DnaK Lla ) and Escherichia coli (DnaK Eco ) were produced in L. lactis NZ9000. In comparison to a strain harboring the empty vector pNZ8048 (designated NZ-Vector) and one expressing dnaKLla (designated NZ-LDnaK), the dnaKEco -expressing strain, named NZ-EDnaK, exhibited more tolerance to heat stress at 40°C in GM17 liquid medium. The cell viability of NZ-Vector was reduced 4.6-fold after 6 h of heat treatment. However, NZ-EDnaK showed 13.5-fold increased viability under these conditions, with a very low concentration of DnaK Eco production. Although the heterologous expression of dnaKEco did not effect DnaK Lla production, heat treatment increased the DnaK Lla level 3.5- and 3.6-fold in NZ-Vector and NZ-EDnaK, respectively. Moreover, NZ-EDnaK showed tolerance to multiple stresses, including 3% NaCl, 5% ethanol, and 0.5% lactic acid (pH 5.47). In CMG medium, the lactate yield and the maximum lactate productivity of NZ-EDnaK were higher than the corresponding values for NZ-Vector at 30°C. Interestingly, at 40°C, these values of NZ-EDnaK were not significantly different from the corresponding values for the control strain at 30°C. Lactate dehydrogenase (LDH) activity was also found to be stable at 40°C in the presence of DnaK Eco . These findings suggest that the heterologous expression of dnaKEco enhances the quality control of proteins and enzymes, resulting in improved growth and lactic acid fermentation at high temperature.

L(+)-Lactic Acid Production Using Lactobacillus Casei in Solid-State Fermentation

2005 ◽  
Vol 27 (21) ◽  
pp. 1685-1688 ◽  
Author(s):  
Pappy John Rojan ◽  
Kesavan Madhavan Nampoothiri ◽  
Athira Syamaprasad Nair ◽  
Ashok Pandey
Keyword(s):  
Lactic Acid ◽  
Solid State ◽  
Solid State Fermentation ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Lactic Acid Production

Engineering Lactococcus lactis for D-Lactic Acid Production from Starch

2019 ◽  
Vol 76 (10) ◽  
pp. 1186-1192 ◽  
Author(s):  
Yuji Aso ◽  
Ayaka Hashimoto ◽  
Hitomi Ohara
Keyword(s):  
Lactic Acid ◽  
Lactococcus Lactis ◽  
Acid Production ◽  
Lactic Acid Production
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