Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions

2014 ◽  
Vol 151 ◽  
pp. 347-354 ◽  
Author(s):  
Yingying Meng ◽  
Changhong Yao ◽  
Song Xue ◽  
Haibo Yang
Keyword(s):  
Fourier Transform ◽  
Ir Spectroscopy ◽  
Fourier Transform Infrared ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

scholarly journals Determination of Glucose in Dried Serum Samples by Fourier-Transform Infrared Spectroscopy

1999 ◽  
Vol 45 (9) ◽  
pp. 1530-1535 ◽  
Author(s):  
Cyril Petibois ◽  
Vincent Rigalleau ◽  
Anne-Marie Melin ◽  
Annie Perromat ◽  
Georges Cazorla ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Ir Spectroscopy ◽  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Internal Standard ◽  
Fourier Transform Infrared ◽  
Serum Samples ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Abstract Background: Practical improvements are needed to allow measurement of glucose concentrations by Fourier- transform infrared (FT-IR) spectroscopy. We developed a new method that allows determination of the glucose concentration in dried sera. Methods: We studied 32 serum samples after fourfold dilution and desiccation before FT-IR analyses on a spectrometer operated at a resolution of 2.0 cm−1. We integrated all spectral windows at the surface of the spectrum in the C—O region. For comparison, glucose was measured in the sera by a glucose oxidase method. Results: One peak within the spectrum was most specific for glucose (997–1062 cm−1). Its surface integration showed a strong relationship with reference data (r = 0.998; P <0.001). FT-IR analyses of five glucose solutions were performed to determine its specific absorption at the same peak. In this way, glucose concentrations in serum spectra could be measured. For the first time while using FT-IR spectroscopy, no manipulation of spectra nor use of internal standard was necessary to obtain results in high accordance with glucose concentration measured by a conventional (glucose-oxidase) method (Sy|x = 0.25 mmol/L; r = 0.998). Conclusions: FT-IR spectroscopy appears to be an easy and accurate method to determine glucose concentration and could be widely used to simultaneously identify and quantify several metabolites in biological fluids or tissues.

Quick determination of melamine in infant powder and liquid milk by Fourier transform infrared spectroscopy

2014 ◽  
Vol 6 (14) ◽  
pp. 5269-5273 ◽  
Author(s):  
Sana Jawaid ◽  
Farah N. Talpur ◽  
Hassan Imran Afridi ◽  
Shafi M. Nizamani ◽  
Abid A. Khaskheli ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ir Spectroscopy ◽  
Cost Effective ◽  
Fourier Transform Infrared ◽  
Ft Ir ◽  
Liquid Milk ◽  
Quick Determination ◽  
Ft Ir Spectroscopy

A simple, cost-effective and environmentally friendly analytical method was developed for the quantification of melamine (MEL) in liquid milk and infant powder by using transmission Fourier transform infrared (FT-IR) spectroscopy.

scholarly journals Bacterial Typing and Identification Based on Fourier Transform Infrared Spectroscopy

2020 ◽  
Author(s):  
Huayan Yang ◽  
Fangling Wu ◽  
Fuxin Xu ◽  
Keqi Tang ◽  
Chuanfan Ding ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Ir Spectroscopy ◽  
Bacterial Culture ◽  
Clinical Laboratory ◽  
Fourier Transform Infrared ◽  
Identification Accuracy ◽  
Bacterial Typing ◽  
Typical Experiment ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Abstract Fourier transform infrared (FT-IR) spectroscopy is a label-free and highly sensitive technique that provides complete information on the chemical composition of biological samples. The bacterial FT-IR signals are extremely specific and highly reproducible fingerprint-like patterns, making FT-IR an efficient tool for bacterial typing and identification. Due to the low cost and high flux, FT-IR has been widely used in hospital hygiene management for infection control, epidemiological studies, and routine bacterial determination of clinical laboratory values. However, the typing and identification accuracy could be affected by many factors, and the bacterial FT-IR data from different laboratories are usually not comparable. A standard protocol is required to improve the accuracy of FT-IR-based typing and identification. Here, we detail the principles and procedures of bacterial typing and identification based on FT-IR spectroscopy, including bacterial culture, sample preparation, instrument operation, spectra collection, spectra preprocessing, and mathematical data analysis. Without bacterial culture, a typical experiment generally takes <2 h.

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