Enhanced Production of Microalgal Biomass and Lipid as An Environmentally Friendly Biodiesel Feedstock Through Actinomycete Co-Culture in Biogas Digestate Effluent

2021 ◽  
pp. 125446
Author(s):  
Bancha Kumsiri ◽  
Jeeraporn Pekkoh ◽  
Wasu Pathom-aree ◽  
Saisamorn Lumyong ◽  
Kittiya Phinyo ◽  
...  
Keyword(s):  
Environmentally Friendly ◽  
Microalgal Biomass ◽  
Enhanced Production ◽  
Biodiesel Feedstock ◽  
Biogas Digestate

scholarly journals Enhanced production of biomass and lipids by Euglena gracilis via co-culturing with a microalga growth-promoting bacterium, Emticicia sp. EG3

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Tadashi Toyama ◽  
Tsubasa Hanaoka ◽  
Koji Yamada ◽  
Kengo Suzuki ◽  
Yasuhiro Tanaka ◽  
...  
Keyword(s):  
Biomass Production ◽  
Euglena Gracilis ◽  
Culture System ◽  
Municipal Wastewater ◽  
Wastewater Effluent ◽  
Biofuel Production ◽  
Second Step ◽  
Microalgal Biomass ◽  
Enhanced Production ◽  
Growth Promoting

Abstract Background Euglena gracilis, a unicellular flagellated microalga, is regarded as one of the most promising species as microalgal feedstock for biofuels. Its lipids (mainly wax esters) are suitable for biodiesel and jet fuel. Culture of E. gracilis using wastewater effluent will improve the economics of E. gracilis biofuel production. Enhancement of the productivity of E. gracilis biomass is critical to creating a highly efficient biofuels production system. Certain bacteria have been found to promote microalgal growth by creating a favorable microenvironment. These bacteria have been characterized as microalgae growth-promoting bacteria (MGPB). Co-culture of microalgae with MGPB might offer an effective strategy to enhance microalgal biomass production in wastewater effluent culture systems. However, no MGPB has been identified to enhance the growth of E. gracilis. The objectives of this study were, therefore, to isolate and characterize the MGPB effective for E. gracilis and to demonstrate that the isolated MGPB indeed enhances the production of biomass and lipids by E. gracilis in wastewater effluent culture system. Results A bacterium, Emticicia sp. EG3, which is capable of promoting the growth of microalga E. gracilis, was isolated from an E. gracilis-municipal wastewater effluent culture. Biomass production rate of E. gracilis was enhanced 3.5-fold and 3.1-fold by EG3 in the co-culture system using a medium of heat-sterilized and non-sterilized wastewater effluent, respectively, compared to growth in the same effluent culture but without EG3. Two-step culture system was examined as follows: E. gracilis was cultured with or without EG3 in wastewater effluent in the first step and was further grown in wastewater effluent in the second step. Production yields of biomass and lipids by E. gracilis were enhanced 3.2-fold and 2.9-fold, respectively, in the second step of the system in which E. gracilis was co-cultured with EG3 in the first step. Conclusion Emticicia sp. EG3 is the first MGPB for E. gracilis. Growth-promoting bacteria such as EG3 will be promising agents for enhancing E. gracilis biomass/biofuel productivities.

Enhanced production of phenol and debromination by co-pyrolysis of the non-metallic fraction of printed circuit boards and waste tire

2021 ◽  
Author(s):  
Chuan Ma ◽  
Shogo Kumagai ◽  
Yuko Saito ◽  
Tomohito Kameda ◽  
Toshiaki Yoshioka
Keyword(s):  
Thermal Treatment ◽  
Printed Circuit Boards ◽  
Environmentally Friendly ◽  
Solid Wastes ◽  
Circuit Boards ◽  
Printed Circuit ◽  
Waste Tire ◽  
Enhanced Production ◽  
Environmentally Friendly Process

The thermal treatment of plastic solid wastes has been widely considered as an environmentally friendly process for converting the inherent polymeric units into fuel or chemicals. In the proposed study,...

Green integrated process for mitigation of municipal and industrial liquid and solid waste mixes for enhanced microalgal biomass and lipid synthesis for biodiesel

RSC Advances ◽  
2015 ◽  
Vol 5 (87) ◽  
pp. 70929-70938 ◽  
Author(s):  
Ankush Karemore ◽  
Ramkrishna Sen
Keyword(s):  
Solid Waste ◽  
Biomass Production ◽  
Lipid Synthesis ◽  
Integrated Process ◽  
Microalgal Biomass ◽  
Biodiesel Feedstock

We herein report: a sustainable green integrated process for effective waste mitigation and microalgal biomass production for biodiesel feedstock generation in a biorefinery model.

Activation of cytochrome c to a peroxidase compound I-type intermediate by H2O2: relevance to redox signalling in apoptosis

2004 ◽  
Vol 71 ◽  
pp. 97-106 ◽  
Author(s):  
Mark Burkitt ◽  
Clare Jones ◽  
Andrew Lawrence ◽  
Peter Wardman
Keyword(s):  
Cytochrome C ◽  
Hydroxyl Radical ◽  
Redox Regulation ◽  
Chemotherapeutic Agents ◽  
Tyrosyl Radical ◽  
Compound I ◽  
Definitive Evidence ◽  
Enhanced Production ◽  
Physico Chemical

The release of cytochrome c from mitochondria during apoptosis results in the enhanced production of superoxide radicals, which are converted to H2O2 by Mn-superoxide dismutase. We have been concerned with the role of cytochrome c/H2O2 in the induction of oxidative stress during apoptosis. Our initial studies showed that cytochrome c is a potent catalyst of 2′,7′-dichlorofluorescin oxidation, thereby explaining the increased rate of production of the fluorophore 2′,7′-dichlorofluorescein in apoptotic cells. Although it has been speculated that the oxidizing species may be a ferryl-haem intermediate, no definitive evidence for the formation of such a species has been reported. Alternatively, it is possible that the hydroxyl radical may be generated, as seen in the reaction of certain iron chelates with H2O2. By examining the effects of radical scavengers on 2′,7′-dichlorofluorescin oxidation by cytochrome c/H2O2, together with complementary EPR studies, we have demonstrated that the hydroxyl radical is not generated. Our findings point, instead, to the formation of a peroxidase compound I species, with one oxidizing equivalent present as an oxo-ferryl haem intermediate and the other as the tyrosyl radical identified by Barr and colleagues [Barr, Gunther, Deterding, Tomer and Mason (1996) J. Biol. Chem. 271, 15498-15503]. Studies with spin traps indicated that the oxo-ferryl haem is the active oxidant. These findings provide a physico-chemical basis for the redox changes that occur during apoptosis. Excessive changes (possibly catalysed by cytochrome c) may have implications for the redox regulation of cell death, including the sensitivity of tumour cells to chemotherapeutic agents.

Are BMP Criteria Really Environmentally Friendly

WRPMD'99 ◽  
1999 ◽  
Author(s):  
Larry A. Roesner ◽  
Robert W. Brashear
Keyword(s):  
Environmentally Friendly

Environmentally Friendly Preparation of Magnetic Composites Featuring Proteolytic Properties

INEOS OPEN ◽  
2020 ◽  
Author(s):  
N. A. Samoilova ◽  
Keyword(s):  
Enzyme Immobilization ◽  
Maleic Acid ◽  
Environmentally Friendly ◽  
Synthetic Polymer ◽  
Magnetic Composites ◽  
Interpolyelectrolyte Complex ◽  
Hydrolysis Rates ◽  
Poly Ethylene ◽  
Noncovalent Binding

The enzyme-containing magnetic composites are presented. The magnetic matrix for enzyme immobilization is obtained by sequential application of an amine-containing polysaccharide—chitosan and a synthetic polymer—poly(ethylene-alt-maleic acid) to the magnetite microparticles to form the interpolyelectrolyte complex shell. Then, the enzyme (trypsin) is immobilized by covalent or noncovalent binding. Thus, the suggested composites can be readily obtained in the environmentally friendly manner. The enzyme capacity of the resulting composites reaches 28.0–32.6 mg/g. The maximum hydrolysis rates of the H-Val-Leu-Lys-pNA substrate provided by these composites range within 0.60·10–7–0.77·10–7 M/min.

Method of high frequency therapy at cows' mastitis during machine milking

2020 ◽  
pp. 9-12
Author(s):  
Viktor E. Lyubimov ◽  
Keyword(s):  
Dairy Cows ◽  
Electromagnetic Fields ◽  
High Frequency ◽  
High Efficiency ◽  
Environmentally Friendly ◽  
Ultrahigh Frequency ◽  
Machine Milking ◽  
High Frequency Electromagnetic Field ◽  
Milking Machine ◽  
Methods Of Treatment

Health of dairy cows ensures human health, so it is important that dairy products do not contain antibiotics that are used to treat any inflammation, including mastitis. In Russia at present, the problem of mastitis in cows exists with both attached and loose housing of cows. Mastitis of dairy cows are the great problem in milking husbandry. Losses from mastitis in milk yield can reach 15-20%. The main reasons for the occurrence of nonspecific mastitis of cows are the shortcomings of the working components of the milking machine: the degree of deterioration of the nipple rubber and the violation of machine milking technology (the reduction in pre-milking time and vacuum fluctuations account for 70% of all causes). Treatment of mastitis with antibiotics is effective, but not environmentally friendly and unacceptable. For the treatment of inflammation of the udder, it is necessary to use more environmentally friendly methods of treatment, one of which is the exposure to ultra-high frequency electromagnetic field tested by medicine. Use of three types of medical-mobile milking machines with the same method of exposure to ultrahigh-frequency electromagnetic fields on cow's nipples through electrodes in milking cups: LPDA-1-UHF, LPDA-2-UHF and LPDA-UHF-30 M, is described in the article. Author proved that cows with subclinical forms of mastitis recovered faster during milking with exposure to the ultrahigh frequency than when treated by antibiotics, and milking with the ultrahigh frequency device helps to recover 82% of the affected quarters with clinical forms and 100% of cows with subclinical forms of mastitis or with udder irritation. The high efficiency of the method of exposure to electromagnetic fields of the ultrahigh frequency during machine milking by means of medical-mobile milking machines LPDA-UHF for the prevention and treatment of mastitis of cows was shown.

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