scholarly journals A direct isothermal amplification system adapted for rapid SNP genotyping of multifarious sample types

2018 ◽  
Vol 115 ◽  
pp. 70-76 ◽  
Author(s):  
Xiaonan Liu ◽  
Chao Zhang ◽  
Mengye Zhao ◽  
Kewu Liu ◽  
Hang Li ◽  
...  
Keyword(s):  
Snp Genotyping ◽  
Isothermal Amplification ◽  
Amplification System

scholarly journals Portable and label-free quantitative Loop-mediated Isothermal Amplification (qLAMP) for reliable COVID-19 diagnostics in 3 minutes: An Arduino-based detection system assisted by a pH microelectrode

2021 ◽  
Author(s):  
Mario Moisés Alvarez ◽  
Sergio Bravo-González ◽  
Everardo González-González ◽  
Grissel Trujillo-de Santiago
Keyword(s):  
Real Time ◽  
Detection System ◽  
Genetic Material ◽  
Cost Effective ◽  
Isothermal Amplification ◽  
Diagnostic Methods ◽  
Label Free ◽  
Viral Pathogens ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification System

Loop-mediated isothermal amplification (LAMP) has been recently studied as an alternative method for cost-effective diagnostics in the context of the current COVID-19 pandemic. Recent reports document that LAMP-based diagnostic methods have a comparable sensitivity and specificity to that of RT-qPCR. We report the use of a portable Arduino-based LAMP-based amplification system assisted by pH microelectrodes for the accurate and reliable diagnosis of SARS-CoV-2 during the first 3 minutes of the amplification reaction. We show that this simple system enables a straightforward discrimination between samples containing or not containing artificial SARS-CoV-2 genetic material in the range of 10 to 10,000 copies per 50 μL of reaction mix. We also spiked saliva samples with SARS-CoV-2 synthetic material and corroborated that the LAMP reaction can be successfully monitored in real time using microelectrodes in saliva samples as well. These results may have profound implications for the design of real-time and portable quantitative systems for the reliable detection of viral pathogens including SARS-CoV-2.

Isothermal amplification system based on template-dependent extension

2013 ◽  
Vol 49 (24) ◽  
pp. 2448 ◽  
Author(s):  
Hui Zhou ◽  
Su-Jin Xie ◽  
Song-Bai Zhang ◽  
Guo-Li Shen ◽  
Ru-Qin Yu ◽  
...  
Keyword(s):  
Isothermal Amplification ◽  
Amplification System

Isothermal Amplification Methods for the SNP Genotyping

2019 ◽  
Vol 19 (7) ◽  
pp. 461-472 ◽  
Author(s):  
Somayeh Heidari Sharafdarkolaee ◽  
Pooria Gill ◽  
Majid Motovali-Bashi ◽  
Fatemeh Heidari Sharafdarkolaee
Keyword(s):  
Mutation Detection ◽  
Driving Forces ◽  
Snp Genotyping ◽  
Isothermal Amplification ◽  
Chain Reaction ◽  
Novel Technologies ◽  
Detection Techniques ◽  
Wide Range ◽  
Polymerase Chain ◽  
Thermal Cycler

The demands for genotyping techniques with acceptable precision, accuracy, cost-effectiveness in high throughput formats made driving forces for continuous development of novel technologies. A wide range of mutation detection techniques based on polymerase chain reaction (PCR) have been introduced. The best alternatives were the isothermal amplification technologies that those did not require a thermal cycler. In this review, we aimed to describe the most known isothermal amplification techniques for SNP genotyping.

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