Detection and identification ofTrichophyton tonsuransfrom clinical isolates and hairbrush samples by loop-mediated isothermal amplification system

2016 ◽  
Vol 43 (9) ◽  
pp. 1037-1043 ◽  
Author(s):  
Ayaka Yo ◽  
Mikachi Yamamoto ◽  
Takako Nakayama ◽  
Jun Ishikawa ◽  
Koichi Makimura
Keyword(s):  
Isothermal Amplification ◽  
Clinical Isolates ◽  
Loop Mediated Isothermal Amplification ◽  
Detection And Identification ◽  
Amplification System

Visual and Real-Time Event-Specific Loop-Mediated Isothermal Amplification Based Detection Assays for Bt Cotton Events MON531 and MON15985

2015 ◽  
Vol 98 (5) ◽  
pp. 1207-1214 ◽  
Author(s):  
Gurinder Jit Randhawa ◽  
Rashmi Chhabra ◽  
Rajesh K Bhoge ◽  
Monika Singh
Keyword(s):  
Real Time ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Bt Cotton ◽  
Loop Mediated Isothermal Amplification ◽  
Commercial Cultivation ◽  
Detection And Identification ◽  
Cotton Seeds ◽  
Gm Detection ◽  
Time Loop

Abstract Bt cotton events MON531 and MON15985 are authorized for commercial cultivation in more than 18 countries. In India, four Bt cotton events have been commercialized; more than 95% of total area under genetically modified (GM) cotton cultivation comprises events MON531 and MON15985. The present study reports on the development of efficient event-specific visual and real-time loop-mediated isothermal amplification (LAMP) assays for detection and identification of cotton events MON531 and MON15985. Efficiency of LAMP assays was compared with conventional and real-time PCR assays. Real-time LAMP assay was found time-efficient and most sensitive, detecting up to two target copies within 35 min. The developed real-time LAMP assays, when combined with efficient DNA extraction kit/protocol, may facilitate onsite GM detection to check authenticity of Bt cotton seeds.

scholarly journals Portable and label-free quantitative Loop-mediated Isothermal Amplification (qLAMP) for reliable COVID-19 diagnostics in 3 minutes: An Arduino-based detection system assisted by a pH microelectrode

2021 ◽  
Author(s):  
Mario Moisés Alvarez ◽  
Sergio Bravo-González ◽  
Everardo González-González ◽  
Grissel Trujillo-de Santiago
Keyword(s):  
Real Time ◽  
Detection System ◽  
Genetic Material ◽  
Cost Effective ◽  
Isothermal Amplification ◽  
Diagnostic Methods ◽  
Label Free ◽  
Viral Pathogens ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification System

Loop-mediated isothermal amplification (LAMP) has been recently studied as an alternative method for cost-effective diagnostics in the context of the current COVID-19 pandemic. Recent reports document that LAMP-based diagnostic methods have a comparable sensitivity and specificity to that of RT-qPCR. We report the use of a portable Arduino-based LAMP-based amplification system assisted by pH microelectrodes for the accurate and reliable diagnosis of SARS-CoV-2 during the first 3 minutes of the amplification reaction. We show that this simple system enables a straightforward discrimination between samples containing or not containing artificial SARS-CoV-2 genetic material in the range of 10 to 10,000 copies per 50 μL of reaction mix. We also spiked saliva samples with SARS-CoV-2 synthetic material and corroborated that the LAMP reaction can be successfully monitored in real time using microelectrodes in saliva samples as well. These results may have profound implications for the design of real-time and portable quantitative systems for the reliable detection of viral pathogens including SARS-CoV-2.

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