DEPIS: A combined dielectrophoresis and impedance spectroscopy platform for rapid cell viability and antimicrobial susceptibility analysis

2021 ◽  
pp. 113190
Author(s):  
Pragya Swami ◽  
Ayush Sharma ◽  
Satyam Anand ◽  
Shalini Gupta
Keyword(s):  
Impedance Spectroscopy ◽  
Cell Viability ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Analysis

Rapid antimicrobial susceptibility profiling using impedance spectroscopy

2021 ◽  
pp. 113876
Author(s):  
Pragya Swami ◽  
Gajanand Verma ◽  
Anurag Holani ◽  
Saipriya Kamaraju ◽  
Vikas Manchanda ◽  
...  
Keyword(s):  
Impedance Spectroscopy ◽  
Antimicrobial Susceptibility

Interpretation of the results of antimicrobial susceptibility analysis of Escherichia coli isolates from bovine milk, meat and associated foodstuffs

2005 ◽  
Vol 22 (4) ◽  
pp. 353-358 ◽  
Author(s):  
Vladimír Babák ◽  
Jarmila Schlegelová ◽  
Hana Vlková
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Bovine Milk ◽  
Susceptibility Analysis

Electrical Impedance Spectroscopy Microflow Cytometer for Cell Viability Tests

2018 ◽  
Author(s):  
J.J. Gonzalez-Murillo ◽  
M. Monge-Azemar ◽  
J. Bartoli ◽  
A. Flores ◽  
M. Moreno ◽  
...  
Keyword(s):  
Impedance Spectroscopy ◽  
Cell Viability ◽  
Electrical Impedance ◽  
Electrical Impedance Spectroscopy ◽  
Microflow Cytometer

scholarly journals Use of Impedance Spectroscopy for the Characterization of In-Vitro Osteoblast Cell Response in Porous Titanium Bone Implants

Metals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1077
Author(s):  
Mercè Giner ◽  
Alberto Olmo ◽  
Miguel Hernández ◽  
Paloma Trueba ◽  
Ernesto Chicardi ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Impedance Spectroscopy ◽  
Cell Viability ◽  
Cellular Response ◽  
Stress Shielding ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Size And Morphology ◽  
Porous Substrates

The use of titanium implants with adequate porosity (content, size and morphology) could solve the stress shielding limitations that occur in conventional titanium implants. Experiments to assess the cellular response (adhesion, proliferation and differentiation of osteoblasts) on implants are expensive, time-consuming and delicate. In this work, we propose the use of impedance spectroscopy to evaluate the growth of osteoblasts on porous titanium implants. Osteoblasts cells were cultured on fully-dense and 40 vol.% porous discs with two ranges of pore size (100–200 μm and 355–500 μm) to study cell viability, proliferation, differentiation (Alkaline phosphatase activity) and cell morphology. The porous substrates 40 vol.% (100–200 µm) showed improved osseointegration response as achieved more than 80% of cell viability and higher levels of Cell Differentiation by Alkaline Phosphatase (ALP) at 21 days. This cell behavior was further evaluated observing an increase in the impedance modulus for all study conditions when cells were attached. However, impedance levels were higher on fully-dense due to its surface properties (flat surface) than porous substrates (flat and pore walls). Surface parameters play an important role on the global measured impedance. Impedance is useful for characterizing cell cultures in different sample types.

scholarly journals Epidemiological investigation and antimicrobial susceptibility analysis of mycoplasma in patients with genital manifestations

2019 ◽  
Vol 34 (4) ◽  
Author(s):  
Xiaodong Gu ◽  
Sudong Liu ◽  
Xuemin Guo ◽  
Ruiqiang Weng ◽  
Zhixiong Zhong
Keyword(s):  
Antimicrobial Susceptibility ◽  
Epidemiological Investigation ◽  
Susceptibility Analysis

scholarly journals Prevalence, biosecurity factor and antimicrobial susceptibility analysis of Salmonella species isolated from commercial duck farms in Korea

2020 ◽  
Author(s):  
Tae-Sik Kim ◽  
Gang-San Kim ◽  
Joo-Sung Son ◽  
Van Dam Lai ◽  
In-Pil Mo ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Analysis

Antimicrobial susceptibility of mastitis pathogens of dairy cows in Switzerland

2014 ◽  
Vol 156 (10) ◽  
pp. 483-488 ◽  
Author(s):  
F. Rüegsegger ◽  
J. Ruf ◽  
A. Tschuor ◽  
Y. Sigrist ◽  
M. Rosskopf ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Antimicrobial Susceptibility ◽  
Mastitis Pathogens

Cytoprotective Effect of Tocotrienol-Rich Fraction and α-Tocopherol Vitamin E Isoforms Against Glutamate-Induced Cell Death in Neuronal Cells

2014 ◽  
Vol 84 (3-4) ◽  
pp. 0140-0151 ◽  
Author(s):  
Thilaga Rati Selvaraju ◽  
Huzwah Khaza’ai ◽  
Sharmili Vidyadaran ◽  
Mohd Sokhini Abd Mutalib ◽  
Vasudevan Ramachandran ◽  
...  
Keyword(s):  
Vitamin E ◽  
Cell Viability ◽  
Neuronal Cells ◽  
Positive Control ◽  
Post Treatment ◽  
Mitochondrial Activity ◽  
Before And After ◽  
Pre Treatment ◽  
Tocotrienol Rich Fraction ◽  
Major Mediator

Glutamate is the major mediator of excitatory signals in the mammalian central nervous system. Extreme amounts of glutamate in the extracellular spaces can lead to numerous neurodegenerative diseases. We aimed to clarify the potential of the following vitamin E isomers, tocotrienol-rich fraction (TRF) and α-tocopherol (α-TCP), as potent neuroprotective agents against glutamate-induced injury in neuronal SK-N-SH cells. Cells were treated before and after glutamate injury (pre- and post-treatment, respectively) with 100 - 300 ng/ml TRF/α-TCP. Exposure to 120 mM glutamate significantly reduced cell viability to 76 % and 79 % in the pre- and post-treatment studies, respectively; however, pre- and post-treatment with TRF/α-TCP attenuated the cytotoxic effect of glutamate. Compared to the positive control (glutamate-injured cells not treated with TRF/α-TCP), pre-treatment with 100, 200, and 300 ng/ml TRF significantly improved cell viability following glutamate injury to 95.2 %, 95.0 %, and 95.6 %, respectively (p < 0.05).The isomers not only conferred neuroprotection by enhancing mitochondrial activity and depleting free radical production, but also increased cell viability and recovery upon glutamate insult. Our results suggest that vitamin E has potent antioxidant potential for protecting against glutamate injury and recovering glutamate-injured neuronal cells. Our findings also indicate that both TRF and α-TCP could play key roles as anti-apoptotic agents with neuroprotective properties.

Pretreatment antimicrobial susceptibility testing is cost saving in the eradication of

2003 ◽  
Vol 1 (4) ◽  
pp. 273-278 ◽  
Author(s):  
Marco Romano ◽  
Riccardo Marmo ◽  
Antonio Cuomo ◽  
Teresa De Simone ◽  
Caterina Mucherino ◽  
...  
Keyword(s):  
Cost Saving ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing
Sign in / Sign up

Export Citation Format

Share Document