An in situ quenching electrochemiluminescence biosensor amplified with aptamer recognition-induced multi-DNA release for sensitive detection of pathogenic bacteria

A novel graphene oxide-based ternary magnetic molecularly imprinted polymer hybrid was synthesized for highly efficient enrichment and sensitive detection of microcystins.

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Fate and transport of faecal contamination microbial indicators, pathogenic protozoa and Campylobacter in the artificially recharged fractured aquifer of Salento, Italy

Water Science & Technology ◽

10.2166/wst.2008.183 ◽

2008 ◽

Vol 57 (6) ◽

pp. 849-856 ◽

Cited By ~ 4

Author(s):

R. La Mantia ◽

C. Masciopinto ◽

C. Levantesi ◽

V. Tandoi

Keyword(s):

Pathogenic Bacteria ◽

Fate And Transport ◽

Fractured Aquifer ◽

Specific Detection ◽

Microbial Indicators ◽

Faecal Contamination ◽

Time Required ◽

Indicators Of Faecal Contamination ◽

Campylobacter Spp

The study investigates the fate and transport of microorganisms introduced by artificial groundwater recharge at the Nardò fractured aquifer in Salento, Italy. Microbial indicators of faecal contamination, parasitic protozoa (Giardia and Cryptosporidium) and pathogenic bacteria (Campylobacter spp.), were monitored into injected water and groundwater to test the efficiency of the “natural disinfection” into the fractured aquifer. A remarkable decrease of microbial indicators and pathogens was observed suggesting that pathogens removal or inactivation may be possible during water flow in fractured aquifer. The recently described PNA probe CJE195 (Lehtola et al. 2005) was utilised for the rapid and specific detection of Campylobacter spp. by fluorescence in situ hybridization (FISH) after enrichment. FISH results were consistent with those of traditional cultural method (ISO 17995) applied in parallel: time required for Campylobacter identification was reduced of 4 days.

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The presence and absence of periplasmic rings in bacterial flagellar motors correlates with stator type

eLife ◽

10.7554/elife.43487 ◽

2019 ◽

Vol 8 ◽

Cited By ~ 17

Author(s):

Mohammed Kaplan ◽

Debnath Ghosal ◽

Poorna Subramanian ◽

Catherine M Oikonomou ◽

Andreas Kjaer ◽

...

Keyword(s):

Legionella Pneumophila ◽

Pathogenic Bacteria ◽

Bioinformatics Analysis ◽

Cell Envelope ◽

Shewanella Oneidensis ◽

Flagellar Motor ◽

Flagellar Motors ◽

A Cell ◽

Animal Hosts

The bacterial flagellar motor, a cell-envelope-embedded macromolecular machine that functions as a cellular propeller, exhibits significant structural variability between species. Different torque-generating stator modules allow motors to operate in different pH, salt or viscosity levels. How such diversity evolved is unknown. Here, we use electron cryo-tomography to determine the in situ macromolecular structures of three Gammaproteobacteria motors: Legionella pneumophila, Pseudomonas aeruginosa, and Shewanella oneidensis, providing the first views of intact motors with dual stator systems. Complementing our imaging with bioinformatics analysis, we find a correlation between the motor’s stator system and its structural elaboration. Motors with a single H+-driven stator have only the core periplasmic P- and L-rings; those with dual H+-driven stators have an elaborated P-ring; and motors with Na+ or Na+/H+-driven stators have both their P- and L-rings embellished. Our results suggest an evolution of structural elaboration that may have enabled pathogenic bacteria to colonize higher-viscosity environments in animal hosts.

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Targeted-Antibacterial-Plasmids (TAPs) combining conjugation and CRISPR/Cas systems achieve strain-specific antibacterial activity

10.1101/2020.10.12.335968 ◽

2020 ◽

Author(s):

Audrey Reuter ◽

Cécile Hilpert ◽

Annick Dedieu-Berne ◽

Sophie Lematre ◽

Erwan Gueguen ◽

...

Keyword(s):

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Bacterial Species ◽

Carbapenem Resistance ◽

Double Strand Breaks ◽

Resistant Bacteria ◽

Innovative Strategy ◽

Strand Breaks ◽

Drug Resistant Bacteria

AbstractThe global emergence of drug-resistant bacteria leads to the loss of efficacy of our antibiotics arsenal and severely limits the success of currently available treatments. Here, we developed an innovative strategy based on Targeted-Antibacterial-Plasmids (TAPs) that use bacterial conjugation to deliver CRISPR/Cas systems exerting a strain-specific antibacterial activity. TAPs are highly versatile as they can be directed against any specific genomic or plasmid DNA using the custom algorithm (CSTB) that identifies appropriate targeting spacer sequences. We demonstrate the ability of TAPs to induce strain-selective killing by introducing lethal double strand breaks (DSBs) into the targeted genomes. TAPs directed against a plasmid-born carbapenem resistance gene efficiently resensitise the strain to the drug. This work represents an essential step towards the development of an alternative to antibiotic treatments, which could be used for in situ microbiota modification to eradicate targeted resistant and/or pathogenic bacteria without affecting other non-targeted bacterial species.

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Wood-derived nanocellulose hydrogel incorporating gold nanoclusters using in situ multistep reactions for efficient sorption and sensitive detection of mercury ion

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.114142 ◽

2021 ◽

Vol 173 ◽

pp. 114142

Author(s):

Qiuyan Luo ◽

Yifeng Huang ◽

Zihua Lei ◽

Junwen Peng ◽

Dong Xu ◽

...

Keyword(s):

Gold Nanoclusters ◽

Mercury Ion ◽

Sensitive Detection ◽

Multistep Reactions

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A novel universal nano-luciferase-involved reporter system for long-term probing food-borne probiotics and pathogenic bacteria in mice by in situ bioluminescence imaging

RSC Advances ◽

10.1039/d0ra01283a ◽

2020 ◽

Vol 10 (22) ◽

pp. 13029-13036 ◽

Cited By ~ 1

Author(s):

Ning Zhao ◽

Jing-Min Liu ◽

Shuang Liu ◽

Xue-Meng Ji ◽

Huan Lv ◽

...

Keyword(s):

Experimental Design ◽

Pathogenic Bacteria ◽

Bioluminescence Imaging ◽

Reporter System ◽

Bioluminescent Bacteria ◽

Foodborne Bacteria ◽

Food Borne

Schematic illustration of the preparation of bioluminescent bacteria and the experimental design of tracing of the foodborne bacteria in vivo.

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CO2 Methanation over Rh/CeO2 Studied with Infrared Modulation Excitation Spectroscopy and Phase Sensitive Detection

Catalysts ◽

10.3390/catal10060601 ◽

2020 ◽

Vol 10 (6) ◽

pp. 601

Author(s):

Felix Hemmingsson ◽

Andreas Schaefer ◽

Magnus Skoglundh ◽

Per-Anders Carlsson

Keyword(s):

Reaction Pathway ◽

Sensitive Detection ◽

Side Reactions ◽

Co2 Methanation ◽

Individual Contributions ◽

Phase Sensitive ◽

Phase Sensitive Detection ◽

Modulation Excitation Spectroscopy ◽

Modulation Excitation

Methane is a well-established fuel molecule whose production from CO 2 through methanation garners increasing interest as an energy storage solution. While often produced with Ni based catalysts, other metals are of interest thanks to higher robustness and activity-selectivity numbers. The Rh/CeO 2 catalyst has shown appreciable properties for CO 2 methanation and its structural dynamics has been studied in situ. However, the reaction pathway is unknown. Here, we present infrared modulation excitation spectroscopy measurements with phase sensitive detection of a Rh/CeO 2 catalyst adsorbate composition during H 2 pulsing (0–2 vol.%) to a constant CO 2 (0.5 vol.%) feed. Various carbonyl (CO) and carbonate (b-CO 3 /p-CO 3 ) ad-species clearly respond to the hydrogen stimulus, making them potential reaction intermediates. The different CO ad-species are likely intermediates for product CO and CH 4 but their individual contributions to the respective formations are not unambiguously ascertained. As for the carbonate dynamics, it might be linked to the reduction/oxidation of the CeO 2 surface upon H 2 pulsing. Formate (HCOO) ad-species are clearly visible but appear to be, if not spectators, linked to slow side reactions possibly also affected by CeO 2 redox processes.

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