(+)-Altholactone exhibits broad spectrum immune modulating activity by inhibiting the activation of pro-inflammatory cytokines in RAW 264.7 cell lines

Acute lung injury (ALI) is a life-threatening syndrome characterized by acute and severe hypoxemic respiratory failure. Visfatin, which is known as an obesity-related cytokine with pro-inflammatory activities, plays a role in regulation of inflammatory cytokines. The mechanisms of ALI remain unclear in critically ill patients. Survival in ALI patients appear to be influenced by the stress generated by mechanical ventilation and by ALI-associated factors that initiate the inflammatory response. The objective for this study was to understand the mechanisms of how visfatin regulates inflammatory cytokines and promotes ALI. The expression of visfatin was evaluated in ALI patients and mouse sepsis models. Moreover, the underlying mechanisms were investigated using human bronchial epithelial cell lines, BEAS-2B and NL-20. An increase of serum visfatin was discovered in ALI patients compared to normal controls. Results from hematoxylin and eosin (H&E) and immunohistochemistry staining also showed that visfatin protein was upregulated in mouse sepsis models. Moreover, lipopolysaccharide (LPS) induced visfatin expression, activated the STAT3/NFκB pathway, and increased the expression of pro-inflammatory cytokines, including IL1-β, IL-6, and TNF-α in human bronchial epithelial cell lines NL-20 and BEAS-2B. Co-treatment of visfatin inhibitor FK866 reversed the activation of the STAT3/NFκB pathway and the increase of pro-inflammatory cytokines induced by LPS. Our study provides new evidence for the involvement of visfatin and down-stream events in acute lung injury. Further studies are required to confirm whether the anti-visfatin approaches can improve ALI patient survival by alleviating the pro-inflammatory process.

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Scopoletin suppresses pro-inflammatory cytokines and PGE2 from LPS-stimulated cell line, RAW 264.7 cells

Fitoterapia ◽

10.1016/j.fitote.2003.12.021 ◽

2004 ◽

Vol 75 (3-4) ◽

pp. 261-266 ◽

Cited By ~ 63

Author(s):

Hyung-Jin Kim ◽

Seon Il Jang ◽

Young-Jun Kim ◽

Hun-Taeg Chung ◽

Yong-Gab Yun ◽

...

Keyword(s):

Cell Line ◽

Inflammatory Cytokines ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Pro Inflammatory Cytokines

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Pandanus fascicularis Lam Extract Inhibits Pro-Inflammatory Cytokines Production in LPS-Stimulated RAW 264.7 Cells

Preventive Nutrition and Food Science ◽

10.3746/pnf.2019.24.3.344 ◽

2019 ◽

Vol 24 (3) ◽

pp. 344-347

Author(s):

Sun-Yup Shim

Keyword(s):

Inflammatory Cytokines ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Pro Inflammatory Cytokines

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Agrimonia pilosa Ledeb Root Extract: Anti-Inflammatory Activities of the Medicinal Herb in LPS-Induced Inflammation

The American Journal of Chinese Medicine ◽

10.1142/s0192415x20500949 ◽

2020 ◽

Vol 48 (08) ◽

pp. 1875-1893

Author(s):

Da-Sol Kim ◽

Kyoung-Eun Park ◽

Yeon-Ju Kwak ◽

Moon-Kyoung Bae ◽

Soo-Kyung Bae ◽

...

Keyword(s):

Porphyromonas Gingivalis ◽

Inflammatory Cytokines ◽

Mapk Signaling ◽

The Body ◽

Raw 264.7 Cells ◽

Root Extract ◽

Raw 264.7 ◽

Cox 2 ◽

Anti Inflammatory ◽

Pro Inflammatory Cytokines

Inflammation regulation is essential for maintaining healthy functions and normal homeostasis of the body. Porphyromonas gingivalis (P. gingivalis) is a gram-negative anaerobic bacterium and a major pathogen that causes oral inflammation and other systemic inflammations. This study aims to examine the anti-inflammatory effects of Agrimonia pilosa Ledeb root extracts (APL-ME) in Porphyromonas gingivalis LPS-induced RAW 264.7 cells and find anti-inflammatory effect compounds of APL-ME. The anti-inflammatory effects of APL-ME were evaluated anti-oxidant activity, cell viability, nitrite concentration, pro-inflammatory cytokines (interleukin-1[Formula: see text], interleukin-6, tumor necrosis factor (TNF)-[Formula: see text], and anti-inflammatory cytokine (interleukin-10 (IL-10)). Also, Inflammation related genes and proteins, cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), expression were decreased by APL-ME and mitogen-activated protein kinase (MAPK) signaling proteins expression was regulated by APL-ME. Liquid chromatography-mass spectrometer (LC/MS)-MS analysis results indicated that several components were detected in APL-ME. Our study indicated that APL-ME suppressed nitrite concentrations, pro-inflammatory cytokines such as IL-1[Formula: see text], IL-6 and TNF-[Formula: see text] in P. gingivalis LPS induced RAW 264.7 cells. However, IL-10 expression was increased by ALP-ME. In addition, protein expressions of COX-2 and iNOS were inhibited APL-ME extracts dose-dependently. According to these results, APL-ME has anti-inflammatory effects in P. gingivalis LPS induced RAW 264.7 cells.

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Polyamine Transport Protein PotD Protects Mice against Haemophilus parasuis and Elevates the Secretion of Pro-Inflammatory Cytokines of Macrophage via JNK–MAPK and NF–κB Signal Pathways through TLR4

Vaccines ◽

10.3390/vaccines7040216 ◽

2019 ◽

Vol 7 (4) ◽

pp. 216 ◽

Cited By ~ 1

Author(s):

Ke Dai ◽

Xiaoyu Ma ◽

Zhen Yang ◽

Yung-Fu Chang ◽

Sanjie Cao ◽

...

Keyword(s):

Immune Responses ◽

Inflammatory Cytokines ◽

Transport System ◽

Signal Pathways ◽

Raw 264.7 ◽

Raw 264.7 Macrophages ◽

Cellular Immune Responses ◽

Polyamine Transport ◽

Cellular Immune ◽

Pro Inflammatory Cytokines

The potD gene, belonging to the well-conserved ABC (ATP-binding cassette) transport system potABCD, encodes the bacterial substrate-binding subunit of the polyamine transport system. In this study, we found PotD in Haemophilus (Glaesserella) parasuis could actively stimulate both humoral immune and cellular immune responses and elevate lymphocyte proliferation, thus eliciting a Th1-type immune response in a murine immunity and infection model. Stimulation of Raw 264.7 macrophages with PotD validated that Toll-like receptor 4, rather than 2, participated in the positive transcription and expression of pro-inflammatory cytokines IL–1β, IL–6, and TNF–α using qPCR and ELISA. Blocking signal-regulated JNK–MAPK and RelA(p65) pathways significantly decreased PotD-induced pro-inflammatory cytokine production. Overall, we conclude that vaccination of PotD could induce both humoral and cellular immune responses and provide immunoprotection against H. parasuis challenge. The data also suggest that Glaesserella PotD is a novel pro-inflammatory mediator and induces TLR4-dependent pro-inflammatory activity in Raw 264.7 macrophages through JNK–MAPK and RelA(p65) pathways.

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