Ulmusakidian, a new coumarin glycoside and antifungal phenolic compounds from the root bark of Ulmus davidiana var. japonica

Introduction: Capparis species (Capparaceae), also called caper, grow naturally in various regions of the world. Caper is a plant with medicinal and aromatic properties. Flower buds, root bark, and fruits of the plant are used in folk medicine due to their analgesic, wound healing, cell regeneration, tonic, and diuretic effects. Objective: The aim of this research was to evaluate in vitro (anti-urease, antioxidant, anticholinesterase) and in vivo (anti-inflammatory) biological activities of caper (C. ovata var. canescens). In addition, we aimed to identify its major phenolic compounds using high performance liquid chromatography with a photodiode array detector (HPLC-DAD) and confirmate them using quadrupole time-of-flight liquid chromatography with tandem mass spectrometry (Q-TOF-LC/MS). Also, we quantified the concentrations of several trace and major elements in plant samples using inductively coupled plasma-mass spectrometry (ICP-MS). Methods: The antioxidant, anti-urease and anticholinesterase activities of different plant extracts were evaluated using DPPH, FRAP, ABTS/TEAC, Indophenol and Ellman tests. The identification of phenolic compounds and trace element contents was performed using HPLC and Q-TOF-LC/MS and ICP-MS. Results: Soxhlet methanol extract exhibited the strongest anti-urease, antioxidant (ABTS/TEAC) and anticholinesterase activity. Soxhlet and maceration methanol extracts demonstrated significant anti-inflammatory effect in the altered edema size after the second hour of carrageenan injection. The active phenolic compounds in Soxhlet methanol extract were identified as rutin, quercetin-hexoside-hexoside, quercetin-3-O-hexoside and kaempferol-3-O-rutinoside. In addition, the average concentrations of vanadium, chromium, manganese, cobalt, copper, nickel, arsenic, selenium, zinc and lead were within the permissible limits defined by WHO for medicinal plants. However, it was found that the concentrations of cadmium and iron were higher than the maximum permissible limits. Conclusion: Our results suggest that although caper has a strong biological activity, it should be consumed carefully due to the excess amount of cadmium and iron elements it contains.

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Structure Related α-Glucosidase Inhibitory Activity and Molecular Docking Analyses of Phenolic Compounds From Paeonia Suffruticosa

10.21203/rs.3.rs-748621/v1 ◽

2021 ◽

Author(s):

Po-Chun Chen ◽

Bongani Sicelo Dlamini ◽

Chiy-Rong Chen ◽

Yueh-Hsiung Kuo ◽

Wen-Ling Shih ◽

...

Keyword(s):

Molecular Docking ◽

Phenolic Compounds ◽

Inhibitory Activity ◽

Inhibitory Effect ◽

Docking Studies ◽

Inhibition Mechanism ◽

Root Bark ◽

Paeonia Suffruticosa ◽

Glucosidase Inhibitors

Abstract In the continuous search for α-glucosidase inhibitors, eleven phenolic compounds (1-11) were isolated from the root bark of Paeonia suffruticosa. Their α-glucosidase inhibitory activity and inhibition mechanism were investigated using an in vitro inhibition assay and molecular docking studies. Compounds 2, 5, 6, and 8-11 (IC50 between 290 and 431 µM) inhibited α-glucosidase more effectively than the reference compound acarbose (IC50=1463 ± 29.5 µM). Among them, compound 10 exhibited the highest α-glucosidase inhibitory effect with an IC50 value of 290.4 ± 9.6 µM. Compounds 2, 5, 9 10 and 11 were found to be competitive inhibitors, while compounds 6 and 8 were noncompetitive inhibitors of α-glucosidase. Computational analyses showed that the main binding forces between the compounds and the main residues were hydrogen bonds. The results indicated that these compounds had considerable α-glucosidase inhibitory activity.

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Bakuchiol, main component of root bark of Ulmus davidiana var. japonica, inhibits TGF-β-induced in vitro EMT and in vivo metastasis

Archives of Biochemistry and Biophysics ◽

10.1016/j.abb.2021.108969 ◽

2021 ◽

pp. 108969

Author(s):

Da-Eun Lee ◽

Eun Hyang Jang ◽

Chaeeun Bang ◽

Gye Lim Kim ◽

So Young Yoon ◽

...

Keyword(s):

Root Bark ◽

Ulmus Davidiana ◽

Main Component

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Antispasmodic phenolic compounds isolated from Morus nigra root bark

10.1055/s-0039-3399964 ◽

2019 ◽

Author(s):

Z Zoofishan ◽

N Kúsz ◽

G Tóth ◽

J Hajagos-Tóth ◽

A Kothencz ◽

...

Keyword(s):

Phenolic Compounds ◽

Root Bark ◽

Morus Nigra

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Antispasmodic Activity of Prenylated Phenolic Compounds from the Root Bark of Morus nigra

Molecules ◽

10.3390/molecules24132497 ◽

2019 ◽

Vol 24 (13) ◽

pp. 2497 ◽

Cited By ~ 3

Author(s):

Zoofishan Zoofishan ◽

Norbert Kúsz ◽

Attila Csorba ◽

Gábor Tóth ◽

Judit Hajagos-Tóth ◽

...

Keyword(s):

Phenolic Compounds ◽

Folk Medicine ◽

Smooth Muscles ◽

Root Bark ◽

Methoxy Derivative ◽

Morus Nigra ◽

Marker Compounds ◽

Related Drug ◽

Nmr Signal Assignment ◽

Smooth Muscle Contractions

Black mulberry is a widely acknowledged ancient traditional medicine. Its extract and constituents have been reported to exert various bioactivities including antimicrobial, hypotensive, analgesic etc. effects. While black mulberry preparations are also used as antispasmodic agents in folk medicine, no related studies are available on its isolated constituents. Through an extensive chromatographic purification, seven phenolic compounds were isolated from the methanol extract of Morus nigra root bark, including morusin (1), kuwanon U (2), kuwanon E (3), moracin P (4), moracin O (5), albanol A (6), and albanol B (7). A complete NMR signal assignment of moracin P and O was achieved, and related literature errors confusing the identity of moracin derivatives are hereby clarified. Compounds 2, 5 and 7 were identified as strong antispasmodic agents on isolated rat ileum and tracheal smooth muscles, while compound 3, a methoxy derivative of 2, was inactive. Moracin O (5) inhibited the ileal and tracheal smooth muscle contractions with Emax values of 85% and 302 mg, respectively. Those actions were superior as compared with papaverine. Our findings demonstrate that prenylated arylbenzofurans, geranylated flavonoids and Diels-Alder adducts from Morus nigra are valuable antispasmodic agents. Compounds 2, 5 and 7 are suggested as marker compounds for quality control of antispasmodic mulberry preparations. Moracin O (5) is a new lead compound for related drug development initiatives.

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Potential Anti-Skin Aging Effect of (-)-Catechin Isolated from the Root Bark of Ulmus davidiana var. japonica in Tumor Necrosis Factor-α-Stimulated Normal Human Dermal Fibroblasts

Antioxidants ◽

10.3390/antiox9100981 ◽

2020 ◽

Vol 9 (10) ◽

pp. 981 ◽

Cited By ~ 1

Author(s):

Sullim Lee ◽

Jae Sik Yu ◽

Hung Manh Phung ◽

Jeong Gun Lee ◽

Ki Hyun Kim ◽

...

Keyword(s):

Tumor Necrosis Factor ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Aging Effect ◽

Skin Aging ◽

Root Bark ◽

Normal Human ◽

Ulmus Davidiana ◽

Factor Α ◽

Necrosis Factor

Reactive oxygen species (ROS) are generated during skin aging, including intrinsic (chronologic aging) and extrinsic aging (photoaging). Therefore, antioxidants that inhibit ROS generation can delay skin aging. In this study, we evaluated the potential anti-skin aging effect of (-)-phenolic compounds isolated from the root bark of Ulmus davidiana var. japonica. We preferentially investigated the possible preventive effects of isolates against the degradation of skin extracellular matrix. Among the isolates, (-)-catechin suppressed the activity of collagenase MMP-1, and reversed the degradation of collagen induced by tumor necrosis factor-α (TNF-α) in normal human dermal fibroblast. This action mechanism of (-)-catechin was validated by the suppression of tumor necrosis factor-α-induced accumulation of ROS and activation of mitogen-activated protein kinases, protein kinase B (Akt), and cyclooxygenase-2 (COX-2). The proinflammatory cytokines upregulate inflammatory reactions, and ultimately promote aging-related reactions. In this milieu, we demonstrated that (-)-catechin decreased the expression and secretion of proinflammatory cytokines, including interleukin (IL)-1β and IL-6. In conclusion, (-)-catechin is a candidate to ameliorate both intrinsic and extrinsic skin aging.

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