Transcriptomic analyses of the acute aerial and ammonia stress response in the gill and liver of large-scale loach (Paramisgurnus dabryanus)

2021 ◽  
Vol 250 ◽  
pp. 109185
Author(s):  
Ze-Hao Shang ◽  
Mei Huang ◽  
Meng-Xiao Wu ◽  
Di Mi ◽  
Kun You ◽  
...  
Keyword(s):  
Stress Response ◽  
Large Scale ◽  
Paramisgurnus Dabryanus ◽  
Ammonia Stress

scholarly journals Phyletic distribution and diversification of the Phage Shock Protein stress response system in bacteria and archaea

2021 ◽  
Author(s):  
Philipp F. Popp ◽  
Vadim M. Gumerov ◽  
Ekaterina P. Andrianova ◽  
Lisa Bewersdorf ◽  
Thorsten Mascher ◽  
...  
Keyword(s):  
Stress Response ◽  
Large Scale ◽  
Cell Envelope ◽  
Model Organisms ◽  
Microbial World ◽  
Natural Classification ◽  
Response System ◽  
Core Proteins ◽  
Envelope Stress

AbstractThe bacterial cell envelope is an essential structure that protects the cell from environmental threats, while simultaneously serving as communication interface and diffusion barrier. Therefore, maintaining cell envelope integrity is of vital importance for all microorganisms. Not surprisingly, evolution has shaped conserved protection networks that connect stress perception, transmembrane signal transduction and mediation of cellular responses upon cell envelope stress. The phage shock protein (PSP) stress response is one of such conserved protection networks. Most of the knowledge about the Psp response comes from studies in the Gram-negative model bacterium, Escherichia coli where the Psp system consists of several well-defined protein components. Homologous systems were identified in representatives of Proteobacteria, Actinobacteria, and Firmicutes; however, the Psp system distribution in the microbial world remains largely unknown. By carrying out a large-scale, unbiased comparative genomics analysis, we found components of the Psp system in many bacterial and archaeal phyla and demonstrated that the PSP system deviates dramatically from the proteobacterial prototype. Two of its core proteins, PspA and PspC, have been integrated in various (often phylum-specifically) conserved protein networks during evolution. Based on protein sequence and gene neighborhood analyses of pspA and pspC homologs, we built a natural classification system of PSP networks in bacteria and archaea. We performed a comprehensive in vivo protein interaction screen for the PSP network newly identified in the Gram-positive model organism Bacillus subtilis and found a strong interconnected PSP response system, illustrating the validity of our approach. Our study highlights the diversity of PSP organization and function across many bacterial and archaeal phyla and will serve as foundation for future studies of this envelope stress response beyond model organisms.

scholarly journals Stiffness and Strength Improvement of Geosynthetic-Reinforced Pavement Foundation Using Large-Scale Wheel Test

2020 ◽  
Vol 5 (4) ◽  
pp. 33
Author(s):  
Jason Wright ◽  
S. Sonny Kim ◽  
Bumjoo Kim
Keyword(s):  
Stress Response ◽  
Dynamic Load ◽  
Large Scale ◽  
Material Behavior ◽  
Traffic Conditions ◽  
Load Tests ◽  
Wheel Loading ◽  
Pavement Layers ◽  
Rolling Wheel ◽  
Pavement Foundation

Laboratory cyclic plate load tests are commonly used in the assessment of geosynthetic performance in pavement applications due to the repeatability of testing results and the smaller required testing areas than traditional Accelerated Pavement Testing facilities. While the objective of traditional plate load testing procedure is to closely replicate traffic conditions, the reality is that rolling wheel loads produce different stresses in pavement layers than traditional cyclic plate load tests. This two-fold study investigates the differences between the stress response of subgrade soil from a rolling wheel load (replicating rolling traffic conditions) and a unidirectional dynamic load (replicating traditional plate load test procedures) in order to obtain a more realistic stress response of pavement layers from rolling wheel traffic. Ultimately, results show that the testing specimens that experienced rolling wheel loading had an average of 17% higher pressure measurements in the top of the subgrade than vertically loaded (unidirectional dynamic load) specimens. The second segment of this study is used in conjunction with the first to analyze aggregate base material behavior when using a geosynthetic for reinforcement. The study aimed to determine the difference in the post-trafficked strength and stiffness of pavement foundation. A Dynamic Cone Penetrometer and Light Weight Deflectometer were utilized to determine material changes from this trafficking and revealed that all specimens that included a geosynthetic had a higher base stiffness and strength while the specimen with geotextile and geogrid in combination created the highest stiffness and strength after large-scale rolling wheel trafficking.

scholarly journals Experience and activity-dependent control of glucocorticoid receptors during the stress response in large-scale brain networks

Stress ◽  
2020 ◽  
pp. 1-24 ◽  
Author(s):  
Damien Huzard ◽  
Virginie Rappeneau ◽  
Onno C. Meijer ◽  
Chadi Touma ◽  
Margarita Arango-Lievano ◽  
...  
Keyword(s):  
Stress Response ◽  
Large Scale ◽  
Glucocorticoid Receptors ◽  
Brain Networks ◽  
Activity Dependent

Transcriptome characterization and SSR discovery in large-scale loach Paramisgurnus dabryanus (Cobitidae, Cypriniformes)

Gene ◽  
2015 ◽  
Vol 557 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Caijuan Li ◽  
Qufei Ling ◽  
Chen Ge ◽  
Zhuqing Ye ◽  
Xiaofei Han
Keyword(s):  
Large Scale ◽  
Paramisgurnus Dabryanus

scholarly journals Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the First Week of Germination

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1878
Author(s):  
Kristýna Kundrátová ◽  
Martin Bartas ◽  
Petr Pečinka ◽  
Ondřej Hejna ◽  
Andrea Rychlá ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Stress Responses ◽  
Proteomic Analysis ◽  
Large Scale ◽  
Crop Yields ◽  
Opium Poppy ◽  
Metabolic Pathway Analysis ◽  
Ubiquitin E3 Ligase ◽  
Key Genes

Water deficiency is one of the most significant abiotic stresses that negatively affects growth and reduces crop yields worldwide. Most research is focused on model plants and/or crops which are most agriculturally important. In this research, drought stress was applied to two drought stress contrasting varieties of Papaver somniferum (the opium poppy), a non-model plant species, during the first week of its germination, which differ in responses to drought stress. After sowing, the poppy seedlings were immediately subjected to drought stress for 7 days. We conducted a large-scale transcriptomic and proteomic analysis for drought stress response. At first, we found that the transcriptomic and proteomic profiles significantly differ. However, the most significant findings are the identification of key genes and proteins with significantly different expressions relating to drought stress, e.g., the heat-shock protein family, dehydration responsive element-binding transcription factors, ubiquitin E3 ligase, and others. In addition, metabolic pathway analysis showed that these genes and proteins were part of several biosynthetic pathways most significantly related to photosynthetic processes, and oxidative stress responses. A future study will focus on a detailed analysis of key genes and the development of selection markers for the determination of drought-resistant varieties and the breeding of new resistant lineages.

scholarly journals Application of RNA Interference Technology to Acroporid Juvenile Corals

2021 ◽  
Vol 8 ◽  
Author(s):  
Ikuko Yuyama ◽  
Tomihiko Higuchi ◽  
Michio Hidaka
Keyword(s):  
Gene Expression ◽  
Rna Interference ◽  
Stress Response ◽  
Large Scale ◽  
Elevated Temperatures ◽  
Fluorescent Protein ◽  
Fluorescence Emission ◽  
Pcr Analysis ◽  
Genes Encoding ◽  
Green Fluorescent

Numerous genes involved in calcification, algal endosymbiosis, and the stress response have been identified in corals by large-scale gene expression analysis, but functional analysis of those genes is lacking. There are few experimental examples of gene expression manipulation in corals, such as gene knockdown by RNA interference (RNAi). The purpose of this study is to establish an RNAi method for coral juveniles. As a first trial, the genes encoding green fluorescent protein (GFP, an endogenous fluorophore expressed by corals) and thioredoxin (TRX, a stress response gene) were selected for knockdown. Synthesized double-stranded RNAs (dsRNAs) corresponding to GFP and TRX were transformed into planula larvae by lipofection method to attempt RNAi. Real-time PCR analysis to verify knockdown showed that GFP and TRX expression levels tended to decrease with each dsRNA treatment (not significant). In addition, stress exposure experiments following RNAi treatment revealed that planulae with TRX knockdown exhibited increased mortality at elevated temperatures. In GFP-knockdown corals, decreased GFP fluorescence was observed. However, the effect of GFP-knockdown was confirmed only in the coral at the initial stages of larval metamorphosis into polyps, but not in planulae and 1 month-old budding polyps. This study showed that lipofection RNAi can be applied to coral planulae and polyps after settlement, and that this method provides a useful tool to modify expression of genes involved in stress tolerance and fluorescence emission of the corals.

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