Iridium oxide nanoparticles-based theranostic probe for in vivo tumor imaging and synergistic chem/photothermal treatments of cancer cells

The co-assembled SPION/PA system with its biocompatible and biodegradable properties can be considered as effective nanocomposite system for MR imaging.

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Copper Oxide Nanoparticles Stimulate Cytotoxicity and Apoptosis in Glial Cancer Cell Line

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v17i1.37126 ◽

2018 ◽

Vol 17 (1) ◽

pp. 105-111 ◽

Cited By ~ 5

Author(s):

Nasim Rahmani Kukia ◽

Ardeshir Abbasi ◽

Seyyed Maysam Abtahi Froushani

Keyword(s):

Copper Oxide ◽

Cancer Cells ◽

Cell Line ◽

Cytotoxic Effect ◽

Cancer Cell Line ◽

Copper Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Cuo Nps

Due to cytotoxic potential, Copper Oxide Nanoparticles (CuO NPs) have recently been studied in various in vivo and in culture cell line. Also, CuO has received much attention in cancer therapy. We aimed to evaluate the cytotoxicity of CuO NPs on glial cancer (B92) cell line. B92 cancer cells were cultured with CuO NPs at different concentrations (5, 10, and 20 μg/ml) with 30 and 60 nm particle size. Then, cancer cells were incubated for 24 hrs. The apoptosis and cytotoxicity of cells were estimated by acridine orange/propidium iodide staining and MTT assay, respectively. Both sizes of CuO NPs had cytotoxic effect. Even with the lowest concentration, the cytotoxic impact accommodated 32% of cell apoptosis with 30 nm size. When the concentration of CuO NPs increased, viability decreased and apoptosis increased. However, these amounts have no significant changes in the concentration of 10 to 20 μg/ml between two particle sizes (30 and 60 nm). The IC50 was decreased as the size of particles increased, but there was no significant change. This finding suggests that exposure to CuO NPs had significant cytotoxic effect with the sizes tested when compared to unexposed control in a way that the smaller size and higher concentration exerted the maximum cytotoxic effects. It seems that augmentation may not have any impact on their in vitro cytotoxicity.Dhaka Univ. J. Pharm. Sci. 17(1): 105-111, 2018 (June)

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Synthesis and Characterization of Radiogallium-Labeled Cationic Amphiphilic Peptides as Tumor Imaging Agents

Cancers ◽

10.3390/cancers13102388 ◽

2021 ◽

Vol 13 (10) ◽

pp. 2388

Author(s):

Takeshi Fuchigami ◽

Takeshi Chiga ◽

Sakura Yoshida ◽

Makoto Oba ◽

Yu Fukushima ◽

...

Keyword(s):

Cancer Cells ◽

Tumor Imaging ◽

Pharmacokinetic Profile ◽

Kb Cells ◽

High Accumulation ◽

Amphiphilic Peptides ◽

Amphiphilic Peptide ◽

Low Levels

SVS-1 is a cationic amphiphilic peptide (CAP) that exhibits a preferential cytotoxicity towards cancer cells over normal cells. In this study, we developed radiogallium-labeled SVS-1 (67Ga-NOTA-KV6), as well as two SVS-1 derivatives, with the repeating KV residues replaced by RV or HV (67Ga-NOTA-RV6 and 67Ga-NOTA-HV6). All three peptides showed high accumulation in epidermoid carcinoma KB cells (53–143% uptake/mg protein). Though 67Ga-NOTA-RV6 showed the highest uptake among the three CAPs, its uptake in 3T3-L1 fibroblasts was just as high, indicating a low selectivity. In contrast, the uptake of 67Ga-NOTA-KV6 and 67Ga-NOTA-HV6 into 3T3-L1 cells was significantly lower than that in KB cells. An endocytosis inhibition study suggested that the three 67Ga-NOTA-CAPs follow distinct pathways for internalization. In the biodistribution study, the tumor uptakes were found to be 4.46%, 4.76%, and 3.18% injected dose/g of tissue (% ID/g) for 67Ga-NOTA-KV6, 67Ga-NOTA-RV6, and 67Ga-NOTA-HV6, respectively, 30 min after administration. Though the radioactivity of these peptides in tumor tissue decreased gradually, 67Ga-NOTA-KV6, 67Ga-NOTA-RV6, and 67Ga-NOTA-HV6 reached high tumor/blood ratios (7.7, 8.0, and 3.8, respectively) and tumor/muscle ratios (5.0, 3.3, and 4.0, respectively) 120 min after administration. 67Ga-NOTA-HV6 showed a lower tumor uptake than the two other tracers, but it exhibited very low levels of uptake into peripheral organs. Overall, the replacement of lysine in SVS-1 with other basic amino acids significantly influenced its binding and internalization into cancer cells, as well as its in vivo pharmacokinetic profile. The high accessibility of these peptides to tumors and their ability to target the surface membranes of cancer cells make radiolabeled CAPs excellent candidates for use in tumor theranostics.

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Lanthanide Europium MOF Nanocomposite as the Theranostic Nanoplatform for Microwave Thermo-Chemotherapy and Fluorescence Imaging

10.21203/rs.3.rs-1173765/v1 ◽

2021 ◽

Author(s):

Lirong Zhao ◽

Wei Zhang ◽

Qiong Wu ◽

Changhui Fu ◽

Xiangling Ren ◽

...

Keyword(s):

Liver Cancer ◽

Cancer Cells ◽

Fluorescence Imaging ◽

Tumor Imaging ◽

Drug Loading ◽

Tumor Therapy ◽

Liver Cancer Cells ◽

Good Ability ◽

Metal Organic

Abstract Backgrounds: Microwave sensitization nanoplatform, integrating multiple functional units for improving tumor selectivity, is of great significance for clinical tumor microwave therapy. Lanthanide europium metal organic framework materials (EuMOF) are expected to be a theranostic nanoplatform owing to its specific luminescent properties and microwave sensitization properties. However, it is difficult to be applied to complex biological systems for EuMOF due to its rapid degradation induced by the solvent molecular and ionic environment. In this work, a luminescent EuMOF nanocomposite (EuMOF@ZIF/AP-PEG, named EZAP) was designed, which brought the multifunctional characteristics of microwave sensitization, fluorescence imaging and drug loading. Results Lamellar EuMOF was synthesized by a hydrothermal method. Through the charge adsorption mechanism, the zeolite imidazole framework (ZIF) structure was densely assembled on the surface of EuMOF to realize the protection. Then, through in-situ apatinib drug loading and PEG modification, EZAP nanocomposite was finally obtained. Apatinib (AP) was a kind of chemotherapy drug approved by Food and Drug Administration for clinical use. PEG modification increased long-term circulation of EZAP nanocomposite. The physical and chemical structure and properties of EuMOF@ZIF (EZ) were systematically represented, indicating the successful synthesis of the nanocomposite. The toxic and side effects were negligible at a safe dose. The growth of human liver cancer cells and murine liver cancer cells in vitro was significantly inhibited, and the combined microwave-thermal therapy and chemotherapy in vivo achieved high anti-cancer efficacy. Moreover, EZAP nanocomposite possessed bright red fluorescence, which had good ability for tumor imaging in tumor-bearing mice in vivo. Conclusion Therefore, EZAP nanocomposite showed high microwave sensitization, excellent fluorescence properties and good drug loading capacity, establishing a promising theranostic nanoplatform for tumor therapy and fluorescence imaging. This work proposes a unique strategy to design for the first time a multifunctional nanoplatform with lanthanide metal organic frameworks for tumor treatment and diagnosis in the biological application.

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Development of anti-HER2 fragment antibody conjugated to iron oxide nanoparticles for in vivo HER2-targeted photoacoustic tumor imaging

Nanomedicine Nanotechnology Biology and Medicine ◽

10.1016/j.nano.2015.07.007 ◽

2015 ◽

Vol 11 (8) ◽

pp. 2051-2060 ◽

Cited By ~ 20

Author(s):

Kengo Kanazaki ◽

Kohei Sano ◽

Akira Makino ◽

Yoichi Shimizu ◽

Fumio Yamauchi ◽

...

Keyword(s):

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Tumor Imaging ◽

Oxide Nanoparticles

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Ultrastructural modification of cellular interactions in cancer tumor

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082455 ◽

1978 ◽

Vol 36 (2) ◽

pp. 318-319

Author(s):

N. P. Dmitrieva

Keyword(s):

Cancer Cells ◽

Human Thyroid ◽

Adjacent Cell ◽

Main Role ◽

Cellular Interactions ◽

Electron Microscopic ◽

Cancer Tumor ◽

Normal Human ◽

Characteristic Features

One of the most characteristic features of cancer cells is their ability to metastasia. It is suggested that the modifications of the structure and properties of cancer cells surfaces play the main role in this process. The present work was aimed at finding out what ultrastructural features apear in tumor in vivo which removal of individual cancer cells from the cell population can provide. For this purpose the cellular interactions in the normal human thyroid and cancer tumor of this gland electron microscopic were studied. The tissues were fixed in osmium tetroxide and were embedded in Araldite-Epon.In normal human thyroid the most common type of intercellular contacts was represented by simple junction formed by the parallelalignment of adjacent cell membranees leaving in between an intermembranes space 15-20 nm filled with electronlucid material (Fig. 1a). Sometimes in the basal part of cells dilatations of the intercellular space 40-50 nm wide were found (Fig. 1a). Here the cell surfaces may form single short microvilli.

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Patients with fever of unknown origin (FUO): diagnosis by nuclear medicine imaging

Nuklearmedizin ◽

10.1055/s-0038-1623872 ◽

2001 ◽

Vol 40 (03) ◽

pp. 59-70 ◽

Cited By ~ 13

Author(s):

W. Becker ◽

J. Meiler

Keyword(s):

Nuclear Medicine ◽

Fever Of Unknown Origin ◽

Tumor Imaging ◽

White Blood Cells ◽

Unknown Origin ◽

Final Diagnosis ◽

Recurrent Fever ◽

Nuclear Medicine Imaging

SummaryFever of unknown origin (FUO) in immunocompetent and non neutropenic patients is defined as recurrent fever of 38,3° C or greater, lasting 2-3 weeks or longer, and undiagnosed after 1 week of appropriate evaluation. The underlying diseases of FUO are numerous and infection accounts for only 20-40% of them. The majority of FUO-patients have autoimmunity and collagen vascular disease and neoplasm, which are responsible for about 50-60% of all cases. In this respect FOU in its classical definition is clearly separated from postoperative and neutropenic fever where inflammation and infection are more common. Although methods that use in-vitro or in-vivo labeled white blood cells (WBCs) have a high diagnostic accuracy in the detection and exclusion of granulocytic pathology, they are only of limited value in FUO-patients in establishing the final diagnosis due to the low prevalence of purulent processes in this collective. WBCs are more suited in evaluation of the focus in occult sepsis. Ga-67 citrate is the only commercially available gamma emitter which images acute, chronic, granulomatous and autoimmune inflammation and also various malignant diseases. Therefore Ga-67 citrate is currently considered to be the tracer of choice in the diagnostic work-up of FUO. The number of Ga-67-scans contributing to the final diagnosis was found to be higher outside Germany than it has been reported for labeled WBCs. F-l 8-2’-deoxy-2-fluoro-D-glucose (FDG) has been used extensively for tumor imaging with PET. Inflammatory processes accumulate the tracer by similar mechanisms. First results of FDG imaging demonstrated, that FDG may be superior to other nuclear medicine imaging modalities which may be explained by the preferable tracer kinetics of the small F-l 8-FDG molecule and by a better spatial resolution of coincidence imaging in comparison to a conventional gamma camera.

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Click Chemistry Expedited Radiosynthesis: Sulfur [18F]fluoride Exchange of Aryl Fluorosulfates

10.26434/chemrxiv.10314647.v1 ◽

2019 ◽

Cited By ~ 1

Author(s):

Qinheng Zheng ◽

Hongtao Xu ◽

Hua Wang ◽

Wen-Ge Han Du ◽

Nan Wang ◽

...

Keyword(s):

Click Chemistry ◽

High Performance ◽

Isotopic Exchange ◽

Tumor Imaging ◽

Radiochemical Yield ◽

Exchange Method ◽

Molar Activity ◽

Positron Emission ◽

Sulfur Fluoride

The lack of simple, efficient [18F]fluorination processes and new target-specific organofluorine probes remains the major challenge of fluorine-18-based positron emission tomography (PET). We report here a fast isotopic exchange method for the radiosynthesis of aryl [18F]fluorosulfate based PET agents enabled by the emerging sulfur fluoride exchange (SuFEx) click chemistry. The method has been applied to the fully-automated 18F-radiolabeling of twenty-five structurally diverse aryl fluorosulfates with excellent radiochemical yield (83–100%) and high molar activity (up to 281 GBq µmol–1) at room temperature in 30 seconds. The purification of radiotracers requires no time-consuming high-performance liquid chromatography (HPLC), but rather a simple cartridge filtration. The utility of aryl [18F]fluorosulfate is demonstrated by the in vivo tumor imaging by targeting poly(ADP-ribose) polymerase 1 (PARP1).

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