scholarly journals Is the traditional alkali extraction method valid in isolating chemically distinct humic acid?

2021 ◽  
Vol 6 ◽  
pp. 100077
Author(s):  
Jianmei Zou ◽  
Huichun Zhang ◽  
Dongbei Yue ◽  
Jianzhi Huang
Keyword(s):  
Humic Acid ◽  
Extraction Method ◽  
Alkali Extraction

scholarly journals Effect of Solution pH to Indigosol Blue Adsorption on Humic Acid Isolated from Kalimantan Peat Oil

2019 ◽  
Vol 2 ◽  
pp. 193-195
Author(s):  
Gita Citra Santi ◽  
Maya Rahmayanti
Keyword(s):  
Humic Acid ◽  
Ftir Spectroscopy ◽  
Extraction Method ◽  
Functional Group ◽  
Peat Soil ◽  
Wave Number ◽  
Solution Ph ◽  
Alkali Extraction ◽  
Ftir Characterization

There has been study on the isolated of humic acid and its interaction with indigosol blue. The objectives of study were to isolate humic acid from Kalimantan peat soil and to study pH optimation indigosol blue on humic acid. Isolated of humic acid using alkali extraction method with NaOH as solvent and precipitated with HCl. Humic acid was characterized using FTIR spectroscopy to determine functional group. Based on the result FTIR characterization, adsorption of humic acid was found in the wave number 3140,15 cm-1 which show the vibration of -OH, 2924,09 cm-1  show vibration of aliphatic  -CH, 1705 cm-1  show vibration -C = O of COOH, 1627,92 cm-1 show vibration of C=C aromatic and 1226,73 cm-1 indicate of -OH and CO from -COOH. Based on the result of study, pH optimation of indigosol blue at pH 5.

A solid phase extraction method for analysis of the iron–humic acid complex in natural water

2014 ◽  
Vol 6 (15) ◽  
pp. 5519-5526 ◽  
Author(s):  
Kai Fang ◽  
Dongxing Yuan ◽  
Lei Zhang ◽  
Lifeng Feng ◽  
Yaojin Chen ◽  
...  
Keyword(s):  
Humic Acid ◽  
Solid Phase Extraction ◽  
Natural Water ◽  
Extraction Method ◽  
Solid Phase ◽  
Phase Extraction ◽  
Acid Complex ◽  
Spe Method

A laboratory synthesized Fe-HA complex was used as a standard to develop a simple SPE method for analysis of Fe-HA complex in natural water.

scholarly journals Extraction and characterization of humic acid from plantation’s solid organic waste composts Ekstraksi dan karakterisasi asam humat dari kompos limbah padat organik perkebunan

2016 ◽  
Vol 68 (2) ◽  
Author(s):  
LAKSMITA P.SANTI P SANTI ◽  
D H GOENADI ◽  
H WIDIASTUTI ◽  
N MARDIANA ◽  
. ISROI
Keyword(s):  
Humic Acid ◽  
Extraction Method ◽  
Organic Waste ◽  
Pilot Scale ◽  
Empty Fruit Bunches ◽  
Air Atmosphere ◽  
Naoh Solution ◽  
Molecular Weight Fractions ◽  
Cocoa Pod Husks

Ringkasan Kompos dari limbah padat organik (LPO) perkebunan memiliki kandungan asam humat yang relatif tinggi.  Namun, metode ekstraksi dan karakteristik asam humat asal kompos tersebut belum banyak diteliti.   Oleh karena itu suatu rangkaian penelitian dilakukan dengan tujuan memperoleh paket teknologi ekstraksi dan menetapkan karakteristik asam humat asal kompos tandan kosong kelapa sawit (TKKS), kulit buah kakao (KBK), dan sisa pangkasan teh (SPT). Pengomposan dilakukan melalui tahapan pengumpulan limbah organik padat perkebunan, pencacahan, pencampuran dengan bioaktivator, inkubasi dan pemanenan.   Hasil penelitian menunjukkan bahwa metode ekstraksi konvensional dengan larutan NaOH dalam atmosfer udara dapat digunakan untuk ekstraksi skala semi pilot.  Jumlah asam humat yang dihasilkan dari kompos asal TKKS dan SPT lebih banyak apabila dibandingkan dengan asam humat asal kompos KBK.  Waktu inkubasi pengomposan dan metode ekstraksi dengan gas N2 atau udara yang digunakan tidak berpengaruh nyata terhadap perolehan asam humat.  Pemurnian asam humat asal ketiga jenis LPO perkebunan dengan menggunakan kolom Sephadex G-50 mengindikasikan bahwa asam humat asal kompos TKKS, KBK,  dan SPT tersebut memiliki fraksi bobot molekul rendah serta didominasi oleh asam amino aspartat dan glutamat.  Konsentrasi asam amino dan senyawa karboksilat  tertinggi terdeteksi pada asam humat  asal kompos SPT.  Summary The plantation’s solid organic waste (SOW) composts contain relatively high humic acid  (HA) substances.  However, there is little information on extraction and characteristics of HA from the SOW-originated composts.  An investigation has been conducted to determine  extraction  and characterisation of HA from empty fruit bunches of oil palm (EFBOP), cocoa pod husks (CPH), and tea cutting residues (TCR). Composting was conducted using the method that involved SOW collection, shredding, mixing with bioactivator, incubation, and harvesting. The results showed that conventional extraction method using NaOH solution under air atmosphere  could be used for pilot scale extraction of humic acids (Has).    Amount of humic acid from EFBOP and TCR were higher than that of CPH.  The composting period  and the extraction method under air or N2 gas were not significantly affected  the amount of the humid acid obtained.    Purification of HA extracted from  EFBOP, TCR, and CPH composts by using Sephadex G-50 column  indicated that  EFBOP, TCR, and CPH contained HAs with lower molecular weight fractions and predominated by aspartic and glutamic acids.  The highest concentration of amino acids  and  carboxyl compounds were detected in the TCR-originated compost

PROPERTIES OF HUMIC ACID FRACTIONS IN FOREST HUMUS LAYERS IN BRITISH COLUMBIA

1975 ◽  
Vol 55 (3) ◽  
pp. 381-393 ◽  
Author(s):  
L. E. LOWE ◽  
CARLIEN H. GODKIN
Keyword(s):  
Humic Acid ◽  
Spectral Characteristics ◽  
Ir Absorption ◽  
Total Acidity ◽  
Spectral Absorption ◽  
Vegetative Cover ◽  
Alkali Extraction ◽  
Ir Absorption Spectra ◽  
Absorption Characteristics ◽  
Forest Humus

Thirty-eight chemical and spectral characteristics were determined on humic acid (HA) fractions extracted successively with cold and hot 0.1 N NaOH from F and H horizons under six types of vegetative cover. Some HA properties were significantly affected by extract, with the hot alkali extraction yielding less aromatic and more immature HA fractions. HA properties were relatively unaffected by horizon of origin. Significant differences between sites were observed with respect to H%, S%, atomic H/C, total acidity, molecular weight distribution and IR absorption spectra. Correlation and multiple regression analysis indicated relationships between properties, and suggested that some spectral absorption characteristics may be predicted from chemical composition, and that certain features of composition, related to aromaticity, can be predicted from easily determined spectral characteristics.

scholarly journals Evaluation of the Copper(II) Complexing Ability of Ultrafiltered Humic Acid by the Solvent Extraction Method

2003 ◽  
Vol 19 (9) ◽  
pp. 1277-1280 ◽  
Author(s):  
Hideyuki ITABASHI ◽  
Yusuke KAMATA ◽  
Daisuke KAWAGUCHI ◽  
Hiroshi KAWAMOTO
Keyword(s):  
Humic Acid ◽  
Solvent Extraction ◽  
Extraction Method ◽  
Complexing Ability ◽  
Solvent Extraction Method

scholarly journals Extraction of Polysaccharide from Spirulina and Evaluation of Its Activities

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Bingyue Wang ◽  
Qian Liu ◽  
Yinghong Huang ◽  
Yueling Yuan ◽  
Qianqian Ma ◽  
...  
Keyword(s):  
High Purity ◽  
Spirulina Platensis ◽  
Extraction Method ◽  
Ph Value ◽  
Extraction Process ◽  
Water Soluble ◽  
Hot Water ◽  
Optimal Method ◽  
Alkali Extraction

Background. Polysaccharide of Spirulina platensis (PSP) is a kind of water-soluble polysaccharide extracted from Spirulina platensis. It has been proved to have antitumor, antioxidation, antiaging, and antivirus properties. And it has a promising prospect for wide application. Objective. This study aims to identify an extraction process for high-purity polysaccharide in Spirulina (PSP) through a series of optimization methods and then evaluates its initial antiaging activities. Methods. Four kinds of extraction methods—hot-water extraction, alkali extraction, ultrasonic-assisted extraction, and freeze-thaw extraction—were compared to find the optimal one, which was further optimized by response surface methodology. PSP was obtained after the crude PSP was deproteinized and depigmented. The antiaging effects of PSP were preliminarily evaluated through in vitro cell experiments. Results. The alkali extraction method was determined as the optimal method, with the optimized extraction process consisting of a solid-liquid ratio of 1 : 50, a pH value of 10.25, a temperature of 89.24°C, and a time of 9.99 h. The final PSP contained 71.65% of polysaccharide and 8.54% of protein. At a concentration of 50 μg/mL, PSP exerted a significant promoting effect on the proliferation and traumatic fusion of human immortalized epidermal cells HaCaT. Conclusion. An extraction method for high-purity PSP with a high extraction rate was established, and in vitro results suggest antioxidation and antiaging activities.

scholarly journals Extraction and characterization of humic acid from plantation’s solid organic waste composts Ekstraksi dan karakterisasi asam humat dari kompos limbah padat organik perkebunan

2016 ◽  
Vol 68 (2) ◽  
Author(s):  
LAKSMITA P.SANTI P SANTI ◽  
D H GOENADI ◽  
H WIDIASTUTI ◽  
N MARDIANA ◽  
. ISROI
Keyword(s):  
Humic Acid ◽  
Extraction Method ◽  
Organic Waste ◽  
Pilot Scale ◽  
Empty Fruit Bunches ◽  
Air Atmosphere ◽  
Naoh Solution ◽  
Molecular Weight Fractions ◽  
Cocoa Pod Husks

Ringkasan Kompos dari limbah padat organik (LPO) perkebunan memiliki kandungan asam humat yang relatif tinggi.  Namun, metode ekstraksi dan karakteristik asam humat asal kompos tersebut belum banyak diteliti.   Oleh karena itu suatu rangkaian penelitian dilakukan dengan tujuan memperoleh paket teknologi ekstraksi dan menetapkan karakteristik asam humat asal kompos tandan kosong kelapa sawit (TKKS), kulit buah kakao (KBK), dan sisa pangkasan teh (SPT). Pengomposan dilakukan melalui tahapan pengumpulan limbah organik padat perkebunan, pencacahan, pencampuran dengan bioaktivator, inkubasi dan pemanenan.   Hasil penelitian menunjukkan bahwa metode ekstraksi konvensional dengan larutan NaOH dalam atmosfer udara dapat digunakan untuk ekstraksi skala semi pilot.  Jumlah asam humat yang dihasilkan dari kompos asal TKKS dan SPT lebih banyak apabila dibandingkan dengan asam humat asal kompos KBK.  Waktu inkubasi pengomposan dan metode ekstraksi dengan gas N2 atau udara yang digunakan tidak berpengaruh nyata terhadap perolehan asam humat.  Pemurnian asam humat asal ketiga jenis LPO perkebunan dengan menggunakan kolom Sephadex G-50 mengindikasikan bahwa asam humat asal kompos TKKS, KBK,  dan SPT tersebut memiliki fraksi bobot molekul rendah serta didominasi oleh asam amino aspartat dan glutamat.  Konsentrasi asam amino dan senyawa karboksilat  tertinggi terdeteksi pada asam humat  asal kompos SPT.  Summary The plantation’s solid organic waste (SOW) composts contain relatively high humic acid  (HA) substances.  However, there is little information on extraction and characteristics of HA from the SOW-originated composts.  An investigation has been conducted to determine  extraction  and characterisation of HA from empty fruit bunches of oil palm (EFBOP), cocoa pod husks (CPH), and tea cutting residues (TCR). Composting was conducted using the method that involved SOW collection, shredding, mixing with bioactivator, incubation, and harvesting. The results showed that conventional extraction method using NaOH solution under air atmosphere  could be used for pilot scale extraction of humic acids (Has).    Amount of humic acid from EFBOP and TCR were higher than that of CPH.  The composting period  and the extraction method under air or N2 gas were not significantly affected  the amount of the humid acid obtained.    Purification of HA extracted from  EFBOP, TCR, and CPH composts by using Sephadex G-50 column  indicated that  EFBOP, TCR, and CPH contained HAs with lower molecular weight fractions and predominated by aspartic and glutamic acids.  The highest concentration of amino acids  and  carboxyl compounds were detected in the TCR-originated compost

scholarly journals Humic Acid Reduces the Available Cadmium, Copper, Lead, and Zinc in Soil and Their Uptake by Tobacco

2020 ◽  
Vol 10 (3) ◽  
pp. 1077 ◽  
Author(s):  
Qun Rong ◽  
Kai Zhong ◽  
He Huang ◽  
Chuanzhang Li ◽  
Chaolan Zhang ◽  
...  
Keyword(s):  
Humic Acid ◽  
Sequential Extraction ◽  
Extraction Method ◽  
Bcr Sequential Extraction ◽  
Plant Materials ◽  
Lead And Zinc ◽  
Nicotiana Tabacum L ◽  
Sequential Extraction Method ◽  
The Difference ◽  
Cu And Zn

Tobacco (Nicotiana tabacum L.) is a crop that is able to accumulate metals. In this study, humic acid was selected as a Cd, Cu, Pb, and Zn passivator, and added to calcareous field soil in amounts of 6.4, 10.3, and 14.8 kg·ha−1. Its impact on the soil fractions of the metals in the soil was extracted by the Community Bureau of Reference (BCR) sequential extraction method, and their accumulation of the metals in tobacco leaves was investigated. Application of 14.8 kg·ha−1 humic acid decreased the DTPA-extracted concentrations of Pb, Cd, Zn, and Cu by 39%, 37%, 29%, and 18%, respectively, as compared with untreated soil. The fractions of Cd, Pb, Cu, and Zn in soil were extracted by the BCR sequential extraction method, and the relationship between the difference metal fractions in the soil and the metal contents in the plant materials were analyzed. The exchangeable fractions of Cd, Pb, Cu, and Zn and the reducible fractions of Pb and Cu are the main bioavailable fractions. Additionally, the reducible fractions of Cd and Zn, the oxidizable fractions of Pb, Cu, and Zn, and all residual fractions of metals were nonbioavailable fractions in the soil. The soils were treated with humic acid (HA) to shift bioavailable metals to stable phases that were less bioavailable. The available Cd, Pb, Cu, and Zn were strongly retained in the soil after the application of humic acid, which decreased the uptake in tobacco in the upper, middle, and lower leaves.

Complex formation of Eu(III) with humic acid and polyacrylic acid

2002 ◽  
Vol 90 (9-11) ◽  
Author(s):  
Takumi Kubota ◽  
Osamu Tochiyama ◽  
K. Tanaka ◽  
Yuichi Niibori
Keyword(s):  
Humic Acid ◽  
Solvent Extraction ◽  
Complex Formation ◽  
Comparative Study ◽  
Polyacrylic Acid ◽  
Extraction Method ◽  
Solvent Extraction Method ◽  
Polyelectrolyte Effect

SummaryWith the intention of estimating the effect of the heterogeneous composition in the humic acid separately from its polyelectrolyte effect, comparative study on the interaction of Eu(III) with humic acid and polyacrylic acid has been carried out by the solvent extraction method with 10

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