Bisphenol A and its analogues bisphenol S, bisphenol F and bisphenol AF induce oxidative stress and biomacromolecular damage in human granulosa KGN cells

Chemosphere ◽  
2020 ◽  
Vol 253 ◽  
pp. 126707 ◽  
Author(s):  
Mingquan Huang ◽  
Shuang Liu ◽  
Li Fu ◽  
Xue Jiang ◽  
Meng Yang
Keyword(s):  
Oxidative Stress ◽  
Bisphenol A ◽  
Bisphenol S ◽  
Bisphenol F ◽  
Bisphenol Af

Bisphenol A analogues bisphenol B, bisphenol F, and bisphenol S induce oxidative stress, disrupt daily sperm production, and damage DNA in rat spermatozoa: a comparative in vitro and in vivo study

2019 ◽  
Vol 35 (4) ◽  
pp. 294-303 ◽  
Author(s):  
Asad Ullah ◽  
Madeeha Pirzada ◽  
Sarwat Jahan ◽  
Hizb Ullah ◽  
Muhammad Jamil Khan
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Bisphenol A ◽  
Estrogenic Activity ◽  
Bisphenol S ◽  
Endocrine Disrupting Chemical ◽  
Sperm Dna Damage ◽  
Bisphenol F

Bisphenol A (BPA) is a well-known endocrine-disrupting chemical with estrogenic activity. The widespread exposure of individuals to BPA is suspected to affect a variety of physiological functions, including reproduction, development, and metabolism. Here we report the mechanisms by which BPA and three of its analogues bisphenol B (BPB), bisphenol F (BPF), and bisphenol S (BPS) cause generation of reactive oxygen species (ROS), sperm DNA damage, and oxidative stress in both in vivo and in vitro rat models. Sperm were incubated with different concentrations (1, 10, and 100 µg/L) of BPA and its analogues BPB, BPF, and BPS for 2 h. BPA and its analogues were observed to increase DNA fragmentation, formation of ROS, and affected levels of superoxide dismutase at higher concentration groups. In an in vivo experiment, rats were exposed to different concentrations (5, 25, and 50 mg/kg/day) of BPA, BPB, BPF, and BPS for 28 days. In the higher dose (50 mg/kg/day) treated groups of BPA and its analogues BPB, BPF, and BPS, DNA damage was observed while the motility of sperm was not affected.

scholarly journals Effects of BPA, BPS, and BPF on Oxidative Stress and Antioxidant Enzyme Expression in Bovine Oocytes and Spermatozoa

Genes ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 142
Author(s):  
Mimi Nguyen ◽  
Reem Sabry ◽  
Ola S. Davis ◽  
Laura A. Favetta
Keyword(s):  
Oxidative Stress ◽  
Bisphenol A ◽  
Western Blot ◽  
Enzyme Expression ◽  
Bisphenol S ◽  
Protein Levels ◽  
Bisphenol F ◽  
Mrna And Protein Expression ◽  
Bovine Oocytes ◽  
Ros Scavengers

Bisphenol A (BPA) and its analogs, bisphenol S (BPS) and bisphenol F (BPF), might impact fertility by altering oxidative stress pathways. Here, we hypothesize that bisphenols-induced oxidative stress is responsible for decreased gamete quality. In both female (cumulus-oocyte-complexes—COCs) and male (spermatozoa), oxidative stress was measured by CM-H2DCFDA assay and key ROS scavengers (SOD1, SOD2, GPX1, GPX4, CAT) were quantified at the mRNA and protein levels using qPCR and Western blot (COCs)/immunofluorescence (sperm). Either gamete was treated in five groups: control, vehicle, and 0.05 mg/mL of BPA, BPS, or BPF. Our results show elevated ROS in BPA-treated COCs but decreased production in BPS- and BPF-treated spermatozoa. Additionally, both mRNA and protein expression of SOD2, GPX1, and GPX4 were decreased in BPA-treated COCs (p < 0.05). In sperm, motility (p < 0.03), but not morphology, was significantly altered by bisphenols. SOD1 mRNA expression was significantly increased, while GPX4 was significantly reduced. These results support BPA’s ability to alter oxidative stress in oocytes and, to a lesser extent, in sperm. However, BPS and BPF likely act through different mechanisms.

scholarly journals Bisphenol AF and Bisphenol F Induce Similar Feminizing Effects in Chicken Embryo Testis as Bisphenol A

2020 ◽  
Vol 178 (2) ◽  
pp. 239-250
Author(s):  
Anna Mentor ◽  
Mimmi Wänn ◽  
Björn Brunström ◽  
Maria Jönsson ◽  
Anna Mattsson
Keyword(s):  
Bisphenol A ◽  
Chicken Embryo ◽  
Reproductive Organ ◽  
Testis Size ◽  
Organ Development ◽  
Bisphenol S ◽  
Left Testis ◽  
Bisphenol F ◽  
Bisphenol Af ◽  
Somatic Index

Abstract The plastic component bisphenol A (BPA) impairs reproductive organ development in various experimental animal species. In birds, effects are similar to those caused by other xenoestrogens. Because of its endocrine disrupting activity, BPA is being substituted with other bisphenols in many applications. Using the chicken embryo model, we explored whether the BPA alternatives bisphenol AF (BPAF), bisphenol F (BPF), and bisphenol S (BPS) can induce effects on reproductive organ development similar to those induced by BPA. Embryos were exposed in ovo from embryonic day 4 (E4) to vehicle, BPAF at 2.1, 21, 210, and 520 nmol/g egg, or to BPA, BPF, or BPS at 210 nmol/g egg and were dissected on embryonic day 19. Similar to BPA, BPAF and BPF induced testis feminization, manifested as eg testis-size asymmetry and ovarian-like cortex in the left testis. In the BPS-group, too few males were alive on day 19 to evaluate any effects on testis development. We found no effects by any treatment on ovaries or Müllerian ducts. BPAF and BPS increased the gallbladder-somatic index and BPAF, BPF and BPS caused increased embryo mortality. The overall lowest-observed-adverse-effect level for BPAF was 210 nmol/g egg based on increased mortality, increased gallbladder-somatic index, and various signs of testis feminization. This study demonstrates that the BPA replacements BPAF, BPF, and BPS are embryotoxic and suggests that BPAF is at least as potent as BPA in inducing estrogen-like effects in chicken embryos. Our results support the notion that these bisphenols are not safe alternatives to BPA.

scholarly journals Binding of bisphenol A, bisphenol AF, and bisphenol S on the androgen receptor: Coregulator recruitment and stimulation of potential interaction sites

2017 ◽  
Vol 44 ◽  
pp. 287-302 ◽  
Author(s):  
Lalith Perera ◽  
Yin Li ◽  
Laurel A. Coons ◽  
Rene Houtman ◽  
Rinie van Beuningen ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Bisphenol A ◽  
Potential Interaction ◽  
Bisphenol S ◽  
Interaction Sites ◽  
Bisphenol Af ◽  
Stimulation Of

Bisphenol A, Bisphenol AF, and Bisphenol S - A comparison of pre- and postnatal endpoints

2018 ◽  
Vol 80 ◽  
pp. 32
Author(s):  
Vicki L. Sutherland ◽  
Barry McIntyre ◽  
Helen Cunny ◽  
Suramya Waidyanatha ◽  
Jui-Hua Hsieh ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Bisphenol S ◽  
Bisphenol Af

scholarly journals Bisphenol S and bisphenol F are less disruptive to cardiac electrophysiology and potentially safer for use in medical products, as compared to bisphenol A

2021 ◽  
Author(s):  
Tomas Prudencio ◽  
Luther Swift ◽  
Devon Guerrelli ◽  
Blake Cooper ◽  
Marissa Reilly ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Safety Profile ◽  
Intracellular Signaling ◽  
Cardiac Electrophysiology ◽  
Field Potential ◽  
Production Volume ◽  
Bisphenol S ◽  
Bisphenol F ◽  
Bisphenol Analogues ◽  
Whole Heart

ABSTRACTBackgroundBisphenol A (BPA) is a high-production volume chemical that is commonly used to manufacture consumer and medical-grade plastic products. Due to its ubiquity, the general population can incur daily environmental exposure to BPA, while heightened BPA exposure has been reported in intensive care patients and industrial workers. Due to health concerns, structural analogues are being explored as replacements for BPA.ObjectiveThis study aimed to examine the direct nongenomic effects of BPA on cardiac electrophysiology and compare its safety profile to recently developed alternatives, including BPS (bisphenol S) and BPF (bisphenol F).MethodsWhole-cell voltage-clamp recordings were performed on cell lines transfected with Nav1.5, hERG, or Cav1.2. Results of single channel experiments were validated by conducting electrophysiology studies on human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) and intact, whole heart preparations.ResultsOf the chemicals tested, BPA was the most potent inhibitor of both fast (INa-P) and late (INa-L) sodium channel (IC50 = 55.3 and 23.6 μM, respectively), L-type calcium channel (IC50 = 30.8 μM) and hERG channel current (IC50 = 127 μM). The inhibitory effects of BPA and BPF on L-type calcium channels were supported by microelectrode array recordings, which revealed shortening of the extracellular field potential (akin to QT interval). Further, BPA and BPF exposure impaired atrioventricular conduction in intact, whole heart experiments. BPS did not alter any of the cardiac electrophysiology parameters tested.DiscussionResults of this study demonstrate that BPA and BPF exert an immediate inhibitory effect on cardiac ion channels, and that BPS may be a safer alternative. Intracellular signaling or genomic effects of bisphenol analogues were not investigated; therefore, additional mechanistic studies are necessary to fully elucidate the safety profile of bisphenol analogues on the heart.

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