Bisphenol A, bisphenol S, bisphenol F and bisphenol AF induce different oxidative stress and damage in human red blood cells ( in vitro study)

2020 ◽

Vol 20 (8) ◽

pp. 1321-1327

Author(s):

Saleh M. Abdullah ◽

Hina Rashid

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Red Blood Cells ◽

Blood Cells ◽

In Vitro Study ◽

Limited Data ◽

Human Red Blood Cells ◽

Living Organisms ◽

Human Rbcs

Background: Bisphenol A (BPA) is a xenobiotic that causes oxidative stress in various organs in living organisms. Blood cells are also an endpoint where BPA is known to cause oxidative stress. Blood cells, especially red blood cells (RBCs), are crucial for maintaining homeostasis and overall wellbeing of the organism. They are highly susceptible to oxidative stress induced by xenobiotics. However, there is limited data about the oxidative stress induced by BPA in blood, especially in red blood cells. This study was carried out to evaluate BPA induced oxidative stress in human RBCs in vitro and its amelioration by melatonin. Objective: To find if melatonin exerts a protective effect on the oxidative stress induced by the BPA in human red blood cells in vitro. Methods: The erythrocyte suspensions (2 ml) were divided into six groups and treated with 0, 50, 100, 150, 200, and 250 μg/ml of BPA. Another set of erythrocyte suspension with similar BPA treatment and 50 μM Melatonin per group was also set. Incubations lasted for 12 hrs in the dark. Lipid peroxidation, glutathione, glutathione reductase, catalase, and superoxide dismutase were measured as indicators of oxidative stress. Results: BPA caused a significant increase in lipid peroxidation. A decrease in GSH levels was also observed. The activities of all the studied antioxidants also decreased with BPA treatment. Melatonin was seen to mitigate the oxidative stress induced by BPA. Conclusion: Treatment of red blood cells with BPA caused an increase in oxidative stress, while melatonin decreased the induced oxidative stress.

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Eryptosis-inducing activity of bisphenol A and its analogs in human red blood cells (in vitro study)

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2015.12.057 ◽

2016 ◽

Vol 307 ◽

pp. 328-335 ◽

Cited By ~ 44

Author(s):

Aneta Maćczak ◽

Monika Cyrkler ◽

Bożena Bukowska ◽

Jaromir Michałowicz

Keyword(s):

Bisphenol A ◽

Red Blood Cells ◽

Blood Cells ◽

In Vitro Study ◽

Vitro Study ◽

Human Red Blood Cells

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Bisphenol A analogues bisphenol B, bisphenol F, and bisphenol S induce oxidative stress, disrupt daily sperm production, and damage DNA in rat spermatozoa: a comparative in vitro and in vivo study

Toxicology and Industrial Health ◽

10.1177/0748233719831528 ◽

2019 ◽

Vol 35 (4) ◽

pp. 294-303 ◽

Cited By ~ 15

Author(s):

Asad Ullah ◽

Madeeha Pirzada ◽

Sarwat Jahan ◽

Hizb Ullah ◽

Muhammad Jamil Khan

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Bisphenol A ◽

Estrogenic Activity ◽

Bisphenol S ◽

Endocrine Disrupting Chemical ◽

Sperm Dna Damage ◽

Bisphenol F

Bisphenol A (BPA) is a well-known endocrine-disrupting chemical with estrogenic activity. The widespread exposure of individuals to BPA is suspected to affect a variety of physiological functions, including reproduction, development, and metabolism. Here we report the mechanisms by which BPA and three of its analogues bisphenol B (BPB), bisphenol F (BPF), and bisphenol S (BPS) cause generation of reactive oxygen species (ROS), sperm DNA damage, and oxidative stress in both in vivo and in vitro rat models. Sperm were incubated with different concentrations (1, 10, and 100 µg/L) of BPA and its analogues BPB, BPF, and BPS for 2 h. BPA and its analogues were observed to increase DNA fragmentation, formation of ROS, and affected levels of superoxide dismutase at higher concentration groups. In an in vivo experiment, rats were exposed to different concentrations (5, 25, and 50 mg/kg/day) of BPA, BPB, BPF, and BPS for 28 days. In the higher dose (50 mg/kg/day) treated groups of BPA and its analogues BPB, BPF, and BPS, DNA damage was observed while the motility of sperm was not affected.

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Influence of physical exercise on β-amyloid, α-synuclein and tau accumulation: an in vitro model of oxidative stress in human red blood cells

ARCHIVES ITALIENNES DE BIOLOGIE ◽

10.12871/000398292017124 ◽

2017 ◽

pp. 33-42

Author(s):

Iofrida ◽

S. Daniele ◽

D. Pietrobono

Keyword(s):

Oxidative Stress ◽

Physical Exercise ◽

Red Blood Cells ◽

Blood Cells ◽

In Vitro Model ◽

Human Red Blood Cells ◽

Β Amyloid ◽

Vitro Model

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Melatonin chelates iron and binds directly with phenylhydrazine to provide protection against phenylhydrazine induced oxidative damage in red blood cells along with its antioxidant mechanisms: an in vitro study

Melatonin Research ◽

10.32794/mr11250001 ◽

2018 ◽

Vol 1 (1) ◽

pp. 1-20 ◽

Cited By ~ 3

Author(s):

Sudeshna Paul ◽

Shamreen Naaz ◽

Arnab Kumar Ghosh ◽

Sanatan Mishra ◽

Aindrila Chattopadhyay ◽

...

Keyword(s):

Oxidative Stress ◽

Free Radical ◽

Red Blood Cells ◽

Blood Cells ◽

Molecular Mechanisms ◽

Radical Scavenging ◽

In Vitro Study ◽

Causative Factor ◽

Protective Effects

Oxidative stress is an important causative factor for a number of diseases. Phenylhydrazine (PHZ) is a widely accepted model for studying hemolytic anemia by induction of oxidative stress. In the present study, goat red blood cells (RBCs) were incubated in vitro with PHZ (1mM) to generate oxidative stress. To test whether melatonin exhibits protective effects on PHZ induced RBC damage and to explore its potential molecular mechanisms, different concentrations of melatonin (5, 10, 20 and 40 nmoles/ml) were also included. PHZ caused altered profiles on biomarkers of oxidative stress and antioxidative as well as glucose metabolic enzymes in RBCs. These alterations indicated a development of oxidative stress. Melatonin at a concentration of 40 nmoles/ml provided optimal protection against all alterations induced by PHZ. The important cellular membrane proteins, including spectrin and actin, were also damaged by PHZ and this led to RBC deformation similar to that of observed in severe β-thalassaemia; the RBC deformation was also prevented by melatonin. Binding profiles of melatonin with PHZ and ferrous iron indicated favorable binding of melatonin with both of them, respectively. Thus, in addition to the direct antioxidant and free radical scavenging capability, melatonin also inhibited iron overloading by chelating iron and binding with the PHZ. This action of melatonin further reduces free radical generation. Based on the results, melatonin may provide therapeutic relevance to ß-thalassemia and other hemolytic RBC disorders involving oxidative stress.

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The Relationship Between Extra- and Intracellular Lithium Concentration in Human Red Blood Cells: an in Vitro Study

The British Journal of Psychiatry ◽

10.1192/bjp.131.1.59 ◽

1977 ◽

Vol 131 (1) ◽

pp. 59-62 ◽

Cited By ~ 12

Author(s):

John J. Ratey ◽

Alan G. Mallinger

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Lithium Concentration ◽

In Vitro Study ◽

Individual Variability ◽

Vitro Study ◽

Normal Volunteers ◽

Human Red Blood Cells ◽

The Relationship

SummaryRed blood cells (RBCs) from normal volunteers were incubated in vitro at four different extracellular lithium concentrations. Extracellular lithium concentration affected RBC lithium accumulation in several ways. As extracellular lithium concentration increased, the inter-individual variability of RBC lithium accumulation increased. Furthermore, the ratio of RBC lithium concentration to extracellular lithium concentration (lithium ratio) increased in relation to increasing extracellular lithium concentration. A lack of agreement among the authors of recent reports dealing with the significance of the lithium ratio as a psychobiological measure has been noted. It is suggested that this lack of agreement may be due, in part, to the study of patients with differing levels of extracellular lithium.

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