scholarly journals HIV DNA loads, plasma residual viraemia and risk of virological rebound in heavily treated, virologically suppressed HIV-infected patients

2015 ◽  
Vol 21 (1) ◽  
pp. 103.e7-103.e10 ◽  
Author(s):  
N. Gianotti ◽  
F. Canducci ◽  
L. Galli ◽  
F. Cossarini ◽  
S. Salpietro ◽  
...  
Keyword(s):  
Hiv Dna ◽  
Residual Viraemia

Long-term follow-up of HIV-infected patients on dolutegravir monotherapy

2019 ◽  
Vol 75 (3) ◽  
pp. 675-680
Author(s):  
G Tebano ◽  
C Soulié ◽  
L Schneider ◽  
C Blanc ◽  
R Agher ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Viral Suppression ◽  
Integrase Inhibitors ◽  
Cd4 Cells ◽  
Hiv Dna ◽  
Kaplan Meier ◽  
Long Term Follow Up ◽  
Residual Viraemia

Abstract Background In recent years, dolutegravir monotherapy has been explored as a drug-reduced regimen for HIV patients. Methods This was a retrospective observational study, including patients virologically suppressed for ≥6 months, without previous virological failure (VF) under integrase inhibitors (INIs), who had been switched to dolutegravir monotherapy (50 mg/day). The primary aim was to report the proportion of VF at week 48 (W48) and week 96 (W96) of dolutegravir monotherapy. The evolution from baseline to W48 of residual viraemia on ultra-deep sequencing and HIV DNA was also evaluated. Results Sixty-one patients were included. Prior to switching to dolutegravir monotherapy, they had a median (IQR) of 15.4 (6.5–19.9) years of antiretroviral exposure, 5.8 (3.2–10.3) years of viral suppression and 687 (461–848) CD4+ cells/mm3. They remained on dolutegravir monotherapy for a median (IQR) of 100 (29–148) weeks. Forty-two out of 61 patients (68.9%) reached W48 and 32 out of 61 patients (52.5%) reached W96. VF occurred in three patients, with the emergence of INI resistance. VF occurred before W24 and in patients pre-exposed to INIs. At W48, the probability of VF (Kaplan–Meier analysis) was 5.6% (95% CI = 1.8%–16.4%). The same result was obtained at W96. Detectable residual viraemia did not increase and median HIV DNA did not change significantly (2.4 log/106 cells at baseline and 2.3 log/106 cells at W48). Dolutegravir plasma concentration was above the IC90 in 41/41 samples, from 22 patients. Conclusions Long-term follow-up showed a low risk of VF under dolutegravir monotherapy, in a selected population of patients with previous long-term virological suppression and low HIV reservoir.

HIV DNA and Immune Alteration During Successful HAART

2013 ◽  
Vol 11 (4) ◽  
pp. 255-262 ◽  
Author(s):  
Diallo Mamadou ◽  
Zheng Yu-Huang ◽  
Chen Xia ◽  
He Bo ◽  
Zhou Hua-Ying ◽  
...  
Keyword(s):  
Immune Alteration ◽  
Hiv Dna

Single-Particle Electrochemical Biosensor with DNA Walker Amplification for Ultrasensitive HIV-DNA Counting

2021 ◽  
Vol 93 (10) ◽  
pp. 4506-4512
Author(s):  
Fanwei Luo ◽  
Fei Chen ◽  
Yi Xiong ◽  
Zhen Wu ◽  
Xiuhua Zhang ◽  
...  
Keyword(s):  
Single Particle ◽  
Electrochemical Biosensor ◽  
Hiv Dna ◽  
Dna Walker

scholarly journals Initiating antiretroviral treatment early in infancy has long-term benefits on the HIV reservoir in late childhood and adolescence

2021 ◽  
Author(s):  
Véronique Avettand-Fenoel ◽  
Jérôme Lechenadec ◽  
Mariama Sadjo Diallo ◽  
Marine Fillion ◽  
Adeline Melard ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Ex Vivo ◽  
T Cell Subsets ◽  
Effector Memory ◽  
Childhood And Adolescence ◽  
Older Children ◽  
Peripheral Blood Mononuclear ◽  
Hiv Reservoir ◽  
Hiv Dna ◽  
Cart Initiation

Abstract Background Early combined antiretroviral therapy (cART) limits the total HIV-DNA load in children. However, data on its impact in older children and adolescents remain scarce. This study aims to compare HIV reservoirs in children (5-12 years) and adolescents (13-17 years) who started cART before 6 months (early (E-)group) or after 2 years old (late (L-)group). Methods The ANRS-EP59-CLEAC study prospectively enrolled 76 HIV-1 perinatally-infected patients who reached HIV-RNA<400 copies/mL less than 24 months after cART initiation, regardless of subsequent viral suppression (E-group: 27 children, 9 adolescents; L-group: 19 children, 21 adolescents). Total and integrated HIV-DNA were quantified in blood and in CD4+ T cell subsets. A substudy assessed HIV reservoir inducibility after ex vivo peripheral blood mononuclear cells (PBMCs) stimulation. Results Total HIV-DNA levels were lower in early- than late-treated patients (Children: 2.14 vs 2.87 log cp/million PBMCs, p<0.0001; Adolescents: 2.25 vs 2.74log, p<0.0001). Low reservoir was independently associated with treatment precocity, protective HLA and low cumulative viremia since cART initiation. The 60 participants with undetectable integrated HIV-DNA started cART earlier than the other patients (4 vs 54 months, p=0.03). In those with sustained virological control, transitional memory and effector memory CD4+T cells were less infected in the E-group than in the L-group (p=0.03 and 0.02, respectively). Viral inducibility of reservoir cells after normalization to HIV-DNA levels was similar between the groups. Conclusions Early cART results in a smaller blood HIV reservoir until adolescence, but all tested participants had an inducible reservoir. This deserves cautious consideration for HIV remission strategies.

Nanosurface energy transfer indicating Exo III-propelled stochastic 3D DNA walkers for HIV DNA detection

The Analyst ◽  
2021 ◽  
Author(s):  
Na Wang ◽  
Yong Jian Jiang ◽  
Xu Zhang ◽  
Hua Rong Lin ◽  
Feng Cheng ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Signal Amplification ◽  
Dna Detection ◽  
Hiv Dna ◽  
Dna Walker ◽  
Exo Iii ◽  
Dna Walkers

An Exo III-propelled stochastic 3D DNA walker based on nanosurface energy transfer was developed for enzyme-assisted DNA detection with signal amplification.

A rapid label- and enzyme-free G-quadruplex-based fluorescence strategy for highly-sensitive detection of HIV DNA

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 206-212 ◽  
Author(s):  
Feng Zhang ◽  
Ling Xiang ◽  
Xianghui Xiao ◽  
Xiaoming Chen ◽  
Chunyan Chen ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Sensitive Detection ◽  
Hiv Dna ◽  
Highly Sensitive ◽  
G Quadruplex ◽  
Highly Sensitive Detection

Because rapid and selective methods for HIV detection are urgently needed, herein, a simple label- and enzyme-free strategy is constructed for fluorescence detection of HIV DNA.

scholarly journals Baseline Cellular HIV DNA Load Predicts HIV DNA Decline and Residual HIV Plasma Levels during Effective Antiretroviral Therapy

2011 ◽  
Vol 50 (2) ◽  
pp. 258-263 ◽  
Author(s):  
S. G. Parisi ◽  
S. Andreis ◽  
C. Mengoli ◽  
R. Scaggiante ◽  
R. Ferretto ◽  
...  
Keyword(s):  
Antiretroviral Therapy ◽  
Plasma Levels ◽  
Hiv Dna

scholarly journals Circulating HIV DNA Correlates With Neurocognitive Impairment in Older HIV-infected Adults on Suppressive ART

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Michelli Faria de Oliveira ◽  
Ben Murrell ◽  
Josué Pérez-Santiago ◽  
Milenka Vargas ◽  
Ronald J. Ellis ◽  
...  
Keyword(s):  
Inflammatory Markers ◽  
Neurocognitive Impairment ◽  
Neurocognitive Functioning ◽  
Neurocognitive Disorder ◽  
Prevention Strategies ◽  
Hiv Dna ◽  
Treatment And Prevention ◽  
Markers Of Inflammation ◽  
Underlying Mechanisms ◽  
Dna Reservoir

Abstract Older HIV-infected adults have a higher risk of neurocognitive impairment, but the underlying mechanisms are poorly understood. Here, we investigated the associations between levels of HIV DNA in peripheral blood, soluble markers of inflammation and cellular trafficking in blood and cerebrospinal fluid (CSF) and neurocognitive functioning among 18 younger (22–40 years) and 26 older (50–71 years) HIV-infected subjects, who were administered a comprehensive neurocognitive battery. Older HIV-infected individuals presented higher levels of inflammation in CSF and blood compared to younger individuals, but no difference was observed in HIV DNA levels. Among older participants, higher HIV DNA levels were significantly associated with more severe neurocognitive impairment (p = 0.005), particularly in the Executive Functions domain (p = 0.004). No association was observed between HIV DNA and neurocognition among younger individuals. Despite significantly increased inflammation observed in the older group, none of the inflammatory markers were associated with neurocognitive impairment among older HIV+ individuals (p > 0.05). Our study supports the involvement of peripheral HIV DNA reservoir in the pathogenesis of neurocognitive disorder during suppressive ART. Correlates of neurocognitive impairment might differ between younger and older adults, suggesting that future treatment and prevention strategies for HIV-associated neurocognitive disorders likely need to be tailored based on age.

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