Bacterial adhesion on hybrid cationic nanoparticle–polymer brush surfaces: Ionic strength tunes capture from monovalent to multivalent binding

2011 ◽  
Vol 87 (1) ◽  
pp. 109-115 ◽  
Author(s):  
Bing Fang ◽  
Saugata Gon ◽  
Myoung Park ◽  
Kushi-Nidhi Kumar ◽  
Vincent M. Rotello ◽  
...  
Keyword(s):  
Ionic Strength ◽  
Bacterial Adhesion ◽  
Polymer Brush ◽  
Multivalent Binding

Bacterial adhesion and growth on a polymer brush-coating

Biomaterials ◽  
2008 ◽  
Vol 29 (30) ◽  
pp. 4117-4121 ◽  
Author(s):  
M. Reza Nejadnik ◽  
Henny C. van der Mei ◽  
Willem Norde ◽  
Henk J. Busscher
Keyword(s):  
Bacterial Adhesion ◽  
Polymer Brush ◽  
Brush Coating

Normally Oriented Adhesion versus Friction Forces in Bacterial Adhesion to Polymer-Brush Functionalized Surfaces Under Fluid Flow

2014 ◽  
Vol 24 (28) ◽  
pp. 4435-4441 ◽  
Author(s):  
Jan J. T. M. Swartjes ◽  
Deepak H. Veeregowda ◽  
Henny C. van der Mei ◽  
Henk J. Busscher ◽  
Prashant K. Sharma
Keyword(s):  
Fluid Flow ◽  
Bacterial Adhesion ◽  
Polymer Brush ◽  
Friction Forces ◽  
Functionalized Surfaces

scholarly journals Determination of the Shear Force at the Balance between Bacterial Attachment and Detachment in Weak-Adherence Systems, Using a Flow Displacement Chamber

2007 ◽  
Vol 74 (3) ◽  
pp. 916-919 ◽  
Author(s):  
M. Reza Nejadnik ◽  
Henny C. van der Mei ◽  
Henk J. Busscher ◽  
Willem Norde
Keyword(s):  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Shear Force ◽  
Polymer Brush ◽  
Bacterial Attachment ◽  
Adhesion Forces ◽  
Shear Forces ◽  
Flow Displacement

ABSTRACT We introduce a procedure for determining shear forces at the balance between attachment and detachment of bacteria under flow. This procedure can be applied to derive adhesion forces in weak-adherence systems, such as polymer brush coatings, which are currently at the center of attention for their control of bacterial adhesion and biofilm formation.

Polyelectrolyte brushes: Water Content, Zeta Potential and Mechanical Properties

2015 ◽  
Vol 1754 ◽  
pp. 53-58
Author(s):  
Joseba Irigoyen ◽  
Jagoba Iturri ◽  
José Luis Camacho ◽  
Edwin Donath ◽  
Sergio Moya
Keyword(s):  
Ionic Strength ◽  
Zeta Potential ◽  
Water Content ◽  
Polymer Brushes ◽  
Polymer Brush ◽  
Trimethylammonium Chloride ◽  
Surface Approach ◽  
Force Microscopy ◽  
Polyelectrolyte Brushes ◽  
Atomic Force

ABSTRACTPolymer brushes of poly[2-(methacryloyloxy)ethyl]trimethylammonium chloride (PMETAC) and poly(sulfo propyl methacrylate) (PSPM) were synthesized by Atomic Transfer Radical Polymerization from planar and colloidal surfaces. Polymer brush growth was followed by QCMD and the water content determined by combined QCMD and elipsometry. From the water content the percentage of water lost during the brush collapse with the ionic strength could be obtained.Highly charged PSPM brushes were indented by Atomic Force Microscopy at different ionic strengths. The force response was fitted to a phenomenological equation analogous to the equation of state of a compressible fluid. Internal energy and brush compressibility were obtained as a function of ionic strength.Spherical brushes of PMETAC and PSPM display an invariance of the zeta potential with ionic strength in the range from 20 mM to 200 mM NaCl, the zeta potential remains almost constant. This invariance can be explained applying a hairy surface approach.

scholarly journals A polysaccharide deacetylase enhances bacterial adhesion in high ionic strength environments

2021 ◽  
Author(s):  
Nelson K Chepkwony ◽  
Yves V Brun
Keyword(s):  
Ionic Strength ◽  
Bacterial Adhesion ◽  
Caulobacter Crescentus ◽  
High Ionic Strength ◽  
Aquatic Environments ◽  
Binding Properties ◽  
Surface Attachment ◽  
Physico Chemical ◽  
Temperature Shear ◽  
Chemical Conditions

The adhesion of organisms to surfaces in aquatic environments provides a diversity of benefits such as better access to nutrients or protection from the elements or from predation. Differences in ionic strength, pH, temperature, shear forces, and other environmental factors impact adhesion and organisms have evolved various strategies to optimize their adhesins for their specific environmental conditions. We know essentially nothing about how bacteria evolved their adhesive mechanisms to attach efficiently in environments with different physico-chemical conditions. Many species of Alphaproteobacteria, including members of the order Caulobacterales, use a polar adhesin, called holdfast, for surface attachment and subsequent biofilm formation in both freshwater and marine environments. Hirschia baltica, a marine member of Caulobacterales, produces a holdfast adhesin that tolerates a drastically higher ionic strength than the holdfast produced by its freshwater relative, Caulobacter crescentus. In this work, we show that the holdfast polysaccharide deacetylase HfsH plays an important role in adherence in high ionic strength environments. We show that deletion of hfsH in H. baltica disrupts holdfast binding properties and structure. Increasing expression of HfsH in C. crescentus improved holdfast binding in high salinity, whereas lowering HfsH expression in H. baltica reduced holdfast binding at high ionic strength. We conclude that HfsH plays a role in modulating holdfast binding at high ionic strength and hypothesize that this modulation occurs through varied deacetylation of holdfast polysaccharides.

scholarly journals Comparative analysis of ionic strength tolerance between freshwater and marine Caulobacterales adhesins

2019 ◽  
Author(s):  
Nelson K. Chepkwony ◽  
Cécile Berne ◽  
Yves V. Brun
Keyword(s):  
Comparative Analysis ◽  
Chemical Composition ◽  
Ionic Strength ◽  
Physicochemical Properties ◽  
Bacterial Adhesion ◽  
Marine Bacterium ◽  
Caulobacter Crescentus ◽  
High Ionic Strength ◽  
Shear Forces ◽  
Surface Attachment

ABSTRACTBacterial adhesion is affected by environmental factors, such as ionic strength, pH, temperature, and shear forces, and therefore marine bacteria must have developed holdfasts with different composition and structures than their freshwater counterparts to adapt to their natural environment. The dimorphic α-proteobacterium Hirschia baltica is a marine budding bacterium in the Caulobacterales clade. H. baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk for surface attachment and cell-cell adhesion. The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast composition and properties in marine Caulobacterales. Here we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales. We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the two genera. We also determine that H. baltica produces larger holdfast than C. crescentus and that those holdfasts have a different chemical composition, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins, but lack DNA. Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus. We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high ionic strength environments.IMPORTANCEMost bacteria spend a large amount of their lifespan attached to surfaces, forming complex multicellular communities called biofilms. Bacteria can colonize virtually any surface, therefore they have adapted to bind efficiently in very different environments. In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica. We show that H. baltica holdfasts have a different morphology and chemical composition, and tolerate high ionic strength. Our results show that H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and providing insights on the physicochemical properties required for adhesion in the marine environment.

scholarly journals Comparative Analysis of Ionic Strength Tolerance between Freshwater and MarineCaulobacteralesAdhesins

2019 ◽  
Vol 201 (18) ◽  
Author(s):  
Nelson K. Chepkwony ◽  
Cécile Berne ◽  
Yves V. Brun
Keyword(s):  
Ionic Strength ◽  
Physicochemical Properties ◽  
Bacterial Adhesion ◽  
Marine Bacterium ◽  
Caulobacter Crescentus ◽  
High Ionic Strength ◽  
Chemical Compositions ◽  
Shear Forces ◽  
Surface Attachment ◽  
Content Type

ABSTRACTBacterial adhesion is affected by environmental factors, such as ionic strength, pH, temperature, and shear forces. Therefore, marine bacteria must have developed adhesins with different compositions and structures than those of their freshwater counterparts to adapt to their natural environment. The dimorphic alphaproteobacteriumHirschia balticais a marine budding bacterium in the cladeCaulobacterales.H. balticauses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion. The holdfast adhesin has been best characterized inCaulobacter crescentus, a freshwater member of theCaulobacterales, and little is known about holdfast compositions and properties in marineCaulobacterales. Here, we useH. balticaas a model to characterize holdfast properties in marineCaulobacterales. We show that freshwater and marineCaulobacteralesuse similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera. We determine thatH. balticaproduces a larger holdfast thanC. crescentusand that the holdfasts have different chemical compositions, as they containN-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA. Finally, we show thatH. balticaholdfasts tolerate higher ionic strength than those ofC. crescentus. We conclude that marineCaulobacteralesholdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCEMost bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms. Bacteria can colonize virtually any surface, and therefore, they have adapted to bind efficiently in very different environments. In this study, we compare the adhesive holdfasts produced by the freshwater bacteriumC. crescentusand a relative, the marine bacteriumH. baltica. We show thatH. balticaholdfasts have a different morphology and chemical composition and tolerate high ionic strength. Our results show that theH. balticaholdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.

scholarly journals Antibacterial Efficacy of Iron-Oxide Nanoparticles against Biofilms on Different Biomaterial Surfaces

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Monica Thukkaram ◽  
Soundarya Sitaram ◽  
Sathish kumar Kannaiyan ◽  
Guruprakash Subbiahdoss
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Polymer Brush ◽  
Oxide Nanoparticles ◽  
Implant Surface ◽  
Biofilm Growth ◽  
Biomaterial Surfaces ◽  
Coated Surfaces

Biofilm growth on the implant surface is the number one cause of the failure of the implants. Biofilms on implant surfaces are hard to eliminate by antibiotics due to the protection offered by the exopolymeric substances that embed the organisms in a matrix, impenetrable for most antibiotics and immune cells. Application of metals in nanoscale is considered to resolve biofilm formation. Here we studied the effect of iron-oxide nanoparticles over biofilm formation on different biomaterial surfaces and pluronic coated surfaces. Bacterial adhesion for 30 min showed significant reduction in bacterial adhesion on pluronic coated surfaces compared to other surfaces. Subsequently, bacteria were allowed to grow for 24 h in the presence of different concentrations of iron-oxide nanoparticles. A significant reduction in biofilm growth was observed in the presence of the highest concentration of iron-oxide nanoparticles on pluronic coated surfaces compared to other surfaces. Therefore, combination of polymer brush coating and iron-oxide nanoparticles could show a significant reduction in biofilm formation.

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