Adipose tissue depot differences in adipokines and effects on skeletal and cardiac muscle

Lipoprotein lipase (LPL) activity and mRNA levels were measured in cardiac muscle and adipose tissue from lean, obese, and weight-stable reduced-obese Zucker rats, both fasted and 2 h after feeding. Fasting epididymal fat LPL activity was substantially higher in obese rats relative to lean rats [6.9 vs. 0.2 nmol free fatty acid (FFA).10(6) cells-1.min-1; P = 0.0001], and was higher still in reduced-obese rats (15.7 nmol FFA.10(6) cells-1.min-1; P = 0.002). Adipose tissue LPL increased with feeding in all three groups. In marked contrast, fasting cardiac muscle LPL was lower in obese rats relative to lean (28.8 vs. 38.5 nmol FFA.g-1.min-1; P = 0.0064) and was lower still in reduced-obese rats (14.5 nmol FFA.g-1.min-1; P = 0.0001). LPL mRNA levels increased in adipose tissue along with enzyme activity; however, the magnitude of the changes were relatively small, suggesting that the primary regulatory steps are posttranslational. Weight reduction studies were also carried out in Sprague-Dawley rats with similar results. These studies show that sustained weight reduction results in coordinate changes in tissue-specific LPL, favoring delivery of lipoprotein triglyceride fatty acids to adipose tissue relative to cardiac muscle and the restoration of energy stores.

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Cold-Induced Thermogenesis Depends on ATGL-Mediated Lipolysis in Cardiac Muscle, but Not Brown Adipose Tissue

Cell Metabolism ◽

10.1016/j.cmet.2017.09.004 ◽

2017 ◽

Vol 26 (5) ◽

pp. 753-763.e7 ◽

Cited By ~ 117

Author(s):

Renate Schreiber ◽

Clemens Diwoky ◽

Gabriele Schoiswohl ◽

Ursula Feiler ◽

Nuttaporn Wongsiriroj ◽

...

Keyword(s):

Adipose Tissue ◽

Brown Adipose Tissue ◽

Cardiac Muscle ◽

Brown Adipose

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Epicardial Adipose Tissue Reflects the Presence of Coronary Artery Disease: Comparison with Abdominal Visceral Adipose Tissue

BioMed Research International ◽

10.1155/2015/483982 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 9

Author(s):

Masayoshi Oikawa ◽

Takashi Owada ◽

Hiroyuki Yamauchi ◽

Tomofumi Misaka ◽

Hirofumi Machii ◽

...

Keyword(s):

Coronary Artery Disease ◽

Adipose Tissue ◽

Coronary Artery ◽

Visceral Adipose Tissue ◽

Coronary Calcification ◽

Atheromatous Plaque ◽

Multivariable Logistic Regression Analysis ◽

Artery Disease ◽

Visceral Adipose ◽

Adipose Tissue Depot

Accumulation of visceral adipose tissue is associated with a risk of coronary artery disease (CAD). The aim of this study was to examine whether different types of adipose tissue depot may play differential roles in the progression of CAD. Consecutive 174 patients who underwent both computed tomography (CT) and echocardiography were analyzed. Cardiac and abdominal CT scans were performed to measure epicardial and abdominal visceral adipose tissue (EAT and abdominal VAT, resp.). Out of 174 patients, 109 and 113 patients, respectively, presented coronary calcification (CC) and coronary atheromatous plaque (CP). The EAT and abdominal VAT areas were larger in patients with CP compared to those without it. Interestingly, the EAT area was larger in patients with CC compared to those without CC, whereas no difference was observed in the abdominal VAT area between patients with CC and those without. Multivariable logistic regression analysis revealed that the presence of echocardiographic EAT was an independent predictor of CP and CC, but the abdominal VAT area was not. These results suggest that EAT and abdominal VAT may play differential pathological roles in CAD. Given the importance of CC and CP, we should consider the precise assessment of CAD when echocardiographic EAT is detected.

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Hepatic and Adipose Tissue Depot-Specific Changes in Lipid Metabolism in Late-Onset Obese (LOB) Rats

Lipids ◽

10.1007/s11745-008-3164-7 ◽

2008 ◽

Vol 43 (4) ◽

pp. 313-324 ◽

Cited By ~ 2

Author(s):

Fredrik Frick ◽

Randip Hume ◽

Iain C. Robinson ◽

Staffan Edén ◽

Jan Oscarsson

Keyword(s):

Adipose Tissue ◽

Lipid Metabolism ◽

Late Onset ◽

Adipose Tissue Depot

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Dermal adipocytes and hair cycling: is spatial heterogeneity a characteristic feature of the dermal adipose tissue depot?

Experimental Dermatology ◽

10.1111/exd.12941 ◽

2016 ◽

Vol 25 (4) ◽

pp. 258-262 ◽

Cited By ~ 30

Author(s):

Ilja L. Kruglikov ◽

Philipp E. Scherer

Keyword(s):

Adipose Tissue ◽

Characteristic Feature ◽

Spatial Heterogeneity ◽

Adipose Tissue Depot

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Вплив гормону росту (rhGH) та Biolaminin 521 LN на проліферативну активність стовбурових клітин кота

Вісник Полтавської державної аграрної академії ◽

10.31210/visnyk2018.03.22 ◽

2018 ◽

pp. 143-148

Author(s):

А. Й. Мазуркевич ◽

В. В. Ковпак ◽

О. С. Ковпак

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Growth Hormone ◽

Adipose Tissue ◽

Cardiac Muscle ◽

Cell Cultures ◽

Human Growth Hormone ◽

Proliferative Activity ◽

Recombinant Human Growth Hormone ◽

Human Growth

Досліджено вплив гормону росту (rhGH) у різних концентраціях та Biolaminin 521 LN на проліферативну активність та генетичну стабільність стовбурових клітин, отриманих з кісткового мозку, жирової тканини та міокарду кота. Встановлено, що гормон росту у низьких концентраціях (10 нг/мл) позитивно впливає на проліферативну активність стовбурових клітин, отриманих із жирової тканини та міокарду кота. Разом із тим, на культуру стовбурових клітин кісткового мозку ефект низьких концентрацій rhGH був протилежний. Культивування стовбурових клітин за додавання Biolaminin 521 LN призвело до достовірного збільшення індексу проліферації у всіх досліджуваних культурах. За даними цитогенетичного аналізу встановлено, що додавання гормону росту у культуральне середовище не призводить до достовірного збільшення кількості генетичних помилок. Водночас, додавання Biolaminin 521 LN призводить до зменшення кількості клітин із зміненим каріотипом (у порівнянні з контролем) у всіх досліджуваних культурах. The usage of cell technology in clinical practice requires a large amount of cell material. It in turn provokes the development of methods that will allow to obtain a greater amount of cell material for a much shorter period of time. According to literary sources it is known that the growth hormone and the Biolaminin LN 521 can have a positive influence on the mitotic activity of stem cells. Considering the differences in the cell composition of the cell cultures obtained from different tissues, the effects of recombinant human growth hormone (rhGH) and Biolaminin 521 LN will be different. Therefore, our aim was to study the effects of recombinant human growth hormone (rhGH) in various concentrations and to study the Biolaminin LN 521 for the proliferative activity of stem cells obtained from bone marrow, adipose tissue and cardiac muscle of cat. The effects of recombinant human growth hormone (rhGH) in various concentrations and the effects of Biolaminin LN 521 for proliferative activity and genetic stability of stem cells obtained from bone marrow, adipose tissue and cardiac muscle of cat were studied. It was found that the growth hormone has a positive effect on the proliferative activity of stem cells in cell cultures of adipose tissue and cardiac muscle of cat at low concentrations (10 ng/ml), while the effect on the cell culture of bone marrow stem cells was the opposite. According to the cytogenetic analysis it was found that adding recombinant human growth hormone to the culture media does not lead to a significant increase in the number of genetic errors, while adding the Biolaminin 521 LN leads to a decrease in the number of cells with altered karyotype (in comparison with control) in all the studied cell cultures.

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MYH9 facilitates autoregulation of adipose tissue depot development

JCI Insight ◽

10.1172/jci.insight.136233 ◽

2021 ◽

Vol 6 (9) ◽

Author(s):

Sin Ying Cheung ◽

Mohd Sayeed ◽

Krishnamurthy Nakuluri ◽

Liang Li ◽

Brian J. Feldman

Keyword(s):

Adipose Tissue ◽

Adipose Tissue Depot

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