scholarly journals Ammonium pyrrolidine dithiocarbamate adsorption on copper surface in neutral chloride media

2016 ◽  
Vol 106 ◽  
pp. 96-107 ◽  
Author(s):  
W. Qafsaoui ◽  
M.W. Kendig ◽  
S. Joiret ◽  
H. Perrot ◽  
H. Takenouti
Keyword(s):  
Copper Surface ◽  
Pyrrolidine Dithiocarbamate ◽  
Ammonium Pyrrolidine Dithiocarbamate

Dithiocarbamate Derivative as an Efficient Inhibitor for Copper

2011 ◽  
Vol 347-353 ◽  
pp. 3724-3727
Author(s):  
Zhong Wen Yue ◽  
Qiang Qiang Liao ◽  
Dong Yang
Keyword(s):  
Corrosion Inhibition ◽  
Anodic Oxidation ◽  
Reduction Process ◽  
Copper Surface ◽  
The Self ◽  
Self Assembled Monolayers ◽  
Pyrrolidine Dithiocarbamate ◽  
Efficient Inhibitor ◽  
Assembled Monolayers ◽  
Ammonium Pyrrolidine Dithiocarbamate

The corrosion inhibition behaviors for the self-assembled monolayers (SAMs) of ammonium pyrrolidine dithiocarbamate（APDTC） on the copper surface in HCl and NaCl solutions had been investigated by polarization curve, respectively. Prone to be adsorbed on the copper surface, the APDTC SAMs restrain both anodic oxidation and cathodic reduction process. The results indicated that APDTC SAMs had good corrosion inhibition effects on copper in both HCl and NaCl solutions.

Determination of Cadmium, Copper, and Lead in Sodium Chloride Food Salts by Flame Atomic Absorption Spectroscopy

1986 ◽  
Vol 69 (5) ◽  
pp. 871-873
Author(s):  
M Jesus Alvarez de Eulate ◽  
Rosa Montoro ◽  
Nieves Ybañez ◽  
Miguel De La Guardia
Keyword(s):  
Sodium Chloride ◽  
Atomic Absorption ◽  
Absorption Spectroscopy ◽  
Atomic Absorption Spectroscopy ◽  
Pyrrolidine Dithiocarbamate ◽  
Flame Atomic Absorption Spectroscopy ◽  
Flame Atomic Absorption ◽  
Coefficients Of Variation ◽  
Ammonium Pyrrolidine Dithiocarbamate

Abstract A method for determination of Cd, Cu, and Pb in sodium chloride food salt samples has been developed. It consists of extraction in 4- methj 1-2-pentanonc of the complexes formed with ammonium pyrrolidine dithiocarbamate and further analysis of the extracts by flame atomic absorption spectroscopy. Detection limits in ng/g salt were 0.2 for Cd, 0.7 for Cu, and 10.0 for Pb. The coefficients of variation of 12 independent analyses were 13% for Cd (at a level of 0.4 ppb), 18% for Cu (1.6 ppb), and 5% for Pb (40 ppb). The recoveries were 100 ± 0% for Cd, 115 ± 14% for Cu, and 100 ± 13% for Pb.

scholarly journals Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis

2019 ◽  
Vol 2 (2) ◽  
pp. 40
Author(s):  
Ezzouhra El Maaiden ◽  
Youssef El Kharrassi ◽  
Abdel Khalid Essamadi ◽  
Khadija Moustaid ◽  
Boubker Nasser
Keyword(s):  
Dna Extraction ◽  
Sodium Dodecyl ◽  
Model Organism ◽  
Tetrahymena Pyriformis ◽  
Pyrrolidine Dithiocarbamate ◽  
Restriction Enzyme Digestion ◽  
Chelex 100 ◽  
Extraction And Purification ◽  
Ammonium Pyrrolidine Dithiocarbamate ◽  
Triton X

Tetrahymena pyriformis (protozoa) is intensely investigated as a model organism, offering numerous advantages in comprehensive and multidisciplinary studies using morphologic or molecular methods. Since DNA extraction is a vital step of any molecular experiment, here a new mixed surfactant (Sodium dodecyl sulfate (SDS) 20%/Triton X-100) was adopted for effective DNA extraction from Tetrahymena pyriformis under an easy, fast protocol. The efficiency of this technique was then compared with three widely-used alternative techniques, namely the Chelex 100 matrix, Ammonium pyrrolidine dithiocarbamate (APD) complex and SDS–chloroform methods. DNA extraction was analyzed by pulsed-field gel electrophoresis, spectral measurement, fluorometry (Qubit), restriction enzyme digestion, and polymerase chain reaction. Data analysis revealed that the quantity and quality of the recovered DNA varied depending on the applied DNA extraction method. The new method (SDS 20%/Triton X-100) was the most efficient for extracting DNA from Tetrahymena pyriformis with high integrity and purity, affordable cost, less time, and suitability for molecular applications.

Experimental Study on Stabilization of Chromium Contaminated Soil

2011 ◽  
Vol 414 ◽  
pp. 273-279
Author(s):  
Ji Da Chen ◽  
Li Liu ◽  
Li Wei Zhang ◽  
Shi Guo Liao ◽  
Yong Ting Song ◽  
...  
Keyword(s):  
Experimental Study ◽  
Contaminated Soil ◽  
Critical Value ◽  
Polluted Soil ◽  
Pyrrolidine Dithiocarbamate ◽  
Stabilization Method ◽  
Toxicity Assay ◽  
Long Time ◽  
Stabilization Effect ◽  
Ammonium Pyrrolidine Dithiocarbamate

To develope a practicable stabilization method for remediation chromium contaminated soil, reductant and chelate reagent were investigated for stabilization artificical chromium contaminated soil, and the stabilization effect was tested through extraction toxicity assay after stablized soil was oxidized at pH 12. The experimental results showed that the composition of sodium bisulfite & ammonium pyrrolidine dithiocarbamate was an ideal stabilizer of chromium in soil, and the extraction toxicity was much less than that of classical stabilized chromium polluted soil with only reductant or the maximum critical value in GB16889-2008, which suggested that the composition of reductant & compound of dithiocarbamate might be practically appllied for remediation chromium contaminated soil because it was likely to remain chromium much more stable in soil for a long time and significantly decrease Cr mobility.

Stimulation of ecto-5′-nucleotidase in human umbilical vein endothelial cells by lipopolysaccharide

2008 ◽  
Vol 295 (3) ◽  
pp. H1177-H1181 ◽  
Author(s):  
R. W. S. Li ◽  
Ricky Y. K. Man ◽  
Paul M. Vanhoutte ◽  
George P. H. Leung
Keyword(s):  
Endothelial Cells ◽  
Messenger Rna ◽  
Umbilical Vein ◽  
Pyrrolidine Dithiocarbamate ◽  
Phosphatidylinositol 3 Kinase ◽  
Human Umbilical Vein ◽  
Inflammatory Damage ◽  
Ammonium Pyrrolidine Dithiocarbamate ◽  
Umbilical Vein Endothelial Cells ◽  
Dose Dependent

The involvement of ecto-5′-nucleotidase (E-5′Nu) in the elevation of extracellular adenosine during inflammation is unclear. In the present study, the effect of lipopolysaccharide (LPS), an inflammation inducer, was investigated on E-5′Nu in human umbilical vein endothelial cells (HUVECs). E-5′Nu activity was enhanced after a 24 h exposure to LPS. This effect was dose dependent, with an EC50 of 1.66 ng/ml. At 10 μM, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY-294002 abolished the LPS-induced E-5′Nu activity. However, at 10 μM, the NF-κB inhibitor ammonium pyrrolidine dithiocarbamate had no effect. LPS upregulated the protein expression but not the messenger RNA expression of E-5′Nu. The inhibition of E-5′Nu by 100 μM α,β-methylene adenosine-5′-diphosphate increased the LPS-induced inflammation, suggesting that E-5′Nu plays a significant role in reducing inflammation, probably through the generation of adenosine. In conclusion, the experiments indicate that LPS upregulates E-5′Nu activity in HUVECs through a PI3K-dependent increase in the abundance of E-5′Nu on cell membranes. Since adenosine is an anti-inflammatory molecule, E-5′Nu upregulation may be crucial in protecting endothelial cells against inflammatory damage.

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