Lysis and biological control of Aspergillus niger by Bacillus subtilis AF 1

1996 ◽  
Vol 42 (6) ◽  
pp. 533-538 ◽  
Author(s):  
A. R. Podile ◽  
A. P. Prakash
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Aspergillus Niger ◽  
Fungal Growth ◽  
Dry Weight ◽  
Crown Rot ◽  
Fungal Mycelium ◽  
Infested Soil ◽  
Dual Culture ◽  
Bacterial Cells

A biocontrol rhizobacterial strain of Bacillus subtilis AF 1 grown for 6 h was coinoculated with Aspergillus niger at different time intervals and microscopic observations revealed adherence of bacterial cells to the fungal mycelium. Bacterial cells multiplied in situ and colonized the mycelial surface. Growth of AF 1 resulted in damage to the cell wall, followed by lysis. AF 1 inoculation into media containing A. niger at 0, 6, and 12 h suppressed >90% fungal growth, while in 18- and 24-h cultures fungal growth inhibition was 70 and 56%, respectively, in terms of dry weight. In dual culture the fungal growth was not accompanied by formation of spores. The mycelial preparation of A. niger as principal carbon source supported the growth of B. subtilis, as much as chitin. Extracellular protein precipitate from B. subtilis culture filtrate had a significant growth-retarding effect on A. niger. Groundnut seeds bacterized with B. subtilis showed a reduced incidence of crown rot in A. niger infested soil, suggesting a possible role of B. subtilis in biological control of A. niger.Key words: mycolytic bacteria, Bacillus subtilis, Aspergillus niger, biological control.

scholarly journals Reduction of Landscape Pathogens in Florida by Soil Solarization

Plant Disease ◽  
2002 ◽  
Vol 86 (12) ◽  
pp. 1388-1395 ◽  
Author(s):  
R. J. McGovern ◽  
R. McSorley ◽  
M. L. Bell
Keyword(s):  
Biological Control ◽  
Crown Rot ◽  
Soil Solarization ◽  
Shoot Biomass ◽  
Infested Soil ◽  
Pseudomonas Chlororaphis ◽  
Biological Control Agents ◽  
Central Florida ◽  
Air Space ◽  
Polyethylene Mulch

Two experiments were conducted during autumn 1997 and 1998 in west-central Florida to evaluate the effectiveness of soil solarization alone and in combination with the biological control agents Streptomyces lydicus (Actinovate) and Pseudomonas chlororaphis (syn. P. aureofasciens, AtEze) and the reduced-risk fungicide fludioxonil (Medallion) in managing soilborne pathogens of impatiens (Impatiens × wallerana, ‘Accent Burgundy’). Naturally infested soil was solarized for 47 or 48 days during September and October using two layers of 25-μm clear, low-density polyethylene mulch, separated by an air space of up to 7.5 cm. Solarization decreased the final incidence and progress of Rhizoctonia crown rot and blight, incidence of Pythium spp. in roots, and root discoloration, and increased shoot biomass in both experiments. The technique also consistently reduced root-knot severity and population densities of Meloidogyne incognita, Dolichodorus heterocephalus, Paratrichodorus minor, and Criconemella spp. The incidence of Rhizoctonia crown rot and blight was reduced by fludioxonil, but not by the biological control agents.

Aflatoxin Production in Black Currant, Blueberry and Strawberry Jams

1978 ◽  
Vol 41 (5) ◽  
pp. 344-347 ◽  
Author(s):  
O. PENSALA ◽  
A. NISKANEN ◽  
S. LINDROTH
Keyword(s):  
Yeast Extract ◽  
Raw Materials ◽  
Fungal Growth ◽  
Dry Weight ◽  
Aflatoxin Production ◽  
Fungal Mycelium ◽  
Black Currant ◽  
Initial Ph ◽  
Different Temperatures ◽  
Yeast Extract Sucrose

Unsweetened and sweetened (20 and 44% sucrose) black currant, blueberry and strawberry jams with spores of Aspergillus parasiticus NRRL 2999 were incubated at different temperatures and atmospheres for 0.5, 1, 2, and 6 months. Hyphal dry weight, pH of medium and aflatoxin production were examined. Also, the aflatoxin distribution between mold and jam layers was examined in jam with uncontrolled and controlled pH (initial pH 3.1–3.6 and 5.6 respectively) and in 20% yeast extract sucrose broth (initial pH 5.6) after 2 weeks of incubation. Aflatoxin was observed in black currant and strawberry jams stored at 22 and 30 C, but not in blueberry jam. Addition of sugar prevented production of aflatoxin in detectable amounts, although it enhanced fungal growth. Storage at 4 C resulted in a marked reduction in fungal growth. The high CO2 atmosphere prevented production of aflatoxin in detectable amounts in black currant and blueberry jams but not in strawberry jam. Raising the initial pH of the stored jam caused an increase in aflatoxin synthesis, although the amount of fungal mycelium, in contrast was reduced. Aflatoxin synthesis as a function of fungal growth was significantly weaker in the jams than in the yeast extract sucrose broth. The results imply that the jam raw materials, particularly blueberry, contain substances inhibiting production of atlatoxins. Alternatively, it is also possible that the jam materials contain only small amounts of nutrients necessary for synthesis of aflatoxin.

scholarly journals Antagonistic effectiveness of some bacteria against Fusarium graminearum causing crown rot disease on wheat (Triticum aestivum)

2021 ◽  
Vol 13 (1) ◽  
pp. 69-80
Author(s):  
Majida Hadi Mahdi Alsaady ◽  
Hussein Ali Salim ◽  
Rakib A. Al-ani ◽  
Hadi M. Aboud ◽  
Jamal Talib M Al Roubaie
Keyword(s):  
Fusarium Graminearum ◽  
Culture Method ◽  
Dry Weight ◽  
Antagonistic Effect ◽  
Crown Rot ◽  
Control Treatment ◽  
Enzyme Analysis ◽  
Dual Culture ◽  
Rot Disease

In this study, the antagonistic effect of five bacteria genera namely Bacillus, Pseudomonas, Azotobacter, Azospirillum, and Streptomyces isolated from rhizosphere of wheat were evaluated against Fusarium graminearum as potential biocontrol agents in vitro. F. graminearum was molecularly diagnosed using the Polymerase chain reaction (PCR) technique. Each bacteria were tested for the production of catalase enzyme, oxidase enzyme, analysis of starch, analyze of gelatin, and the motility, where Azotobacter, Azospirillum, and Bacillus subtilis were positive for all tested. Fungal inhibition tests were performed by using the dual culture method and agar well diffusion technique. Among them, Streptomyces and Azospirillum exhibited potent inhibition to the growth of F. graminearum (72.14% and 66.42%) respectively, followed by B.pumillus, P.fluorescens, B. subtilis and Azotobacter ( 58.28%, 43.23%, 39.71% and 35.71%) respectively as compared with the control treatment (0.0%).The dry weight of the fungus biomass was decreased with bacteria P. fluorescens, Streptomyces sp, Azotobacter sp, Azospirillum sp, B. subtilis, and B. pumillus which reached (0.114, 0.103, 0.147, 0.101, 0.143, and 0.107 g) respectively compared to the control treatment that was 0. 665 g.

scholarly journals Influence of Chloride and Nitrogen Form on Rhizoctonia Root and Crown Rot of Table Beets

Plant Disease ◽  
1997 ◽  
Vol 81 (6) ◽  
pp. 635-640 ◽  
Author(s):  
Wade H. Elmer
Keyword(s):  
Dry Weight ◽  
Anastomosis Group ◽  
Crown Rot ◽  
Infested Soil ◽  
Nitrogen Form ◽  
Root Yield ◽  
Field Soils ◽  
Root And Crown Rot ◽  
The Mean ◽  
Group 2

The effect of NaCl combined with Ca(NO3)2 or (NH4)2SO4 was examined on table beets (Beta vulgaris) in the presence and absence of Rhizoctonia solani (anastomosis group 2-2), the cause of Rhizoctonia root and crown rot. Transplants of cvs. Detroit Dark Red and Early Wonder grown in the greenhouse in infested soils and fertilized with Ca(NO3)2 (10 mmol of N) were 32% larger in dry weight than plants treated with (NH4)2SO4 (10 mmol of N). In noninfested soils, a 17% increase in dry weights was observed for plants treated with Ca(NO3)2 compared to plants that were fed (NH4)2SO4. When NaCl (0.17 mmol) was applied, the mean dry weight sincreased 40% in noninfested soil and 12% in infested soil compared to plants that received no NaCl. No significant interaction occurred between N fertilizer and NaCl in greenhouse trials. However, in field soils infested with R. solani, NaCl (560 kg/ha) combined with (NH4)2SO4 (112kg of N per ha) produced 26 to 47% more root yield than when (NH4)2SO4 was used alone. Inthe absence of NaCl, Ca(NO3)2 suppressed disease more than (NH4)2SO4, but adding NaCl to Ca(NO3)2 did not increase yield more than Ca(NO3)2 alone. The Cl salts KCl, CaCl2, and MgCl2did not significantly differ from NaCl in their ability to increase the dry weight of beets grownin infested soils. Leaf and root analyses revealed that (NH4)2SO4 applications increased N, P, S, and Mn in tissue more than Ca(NO3)2 applications. Applying NaCl increased tissue levels of Na, Cl, and Mn more than in plants that were not fed NaCl. All of the Cl salts had the effect of increasing concentrations of Cl and Mn in the plant. There was no evidence that the Na ion was disease suppressive. Chloride, however, may be of use in disease management of Rhizoctonia root and crown rot of table beets.

Biological control of Aspergillus niger, the cause of Black-rot disease of Allium cepa L. (onion), by Penicillium species

2012 ◽  
Vol 29 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Ibatsam Khokhar ◽  
Muhammad Saleem Haider ◽  
Irum Mukhtar ◽  
Sobia Mushtaq
Keyword(s):  
Biological Control ◽  
Aspergillus Niger ◽  
Agar Plate ◽  
Antagonistic Effect ◽  
Black Rot ◽  
Dual Culture ◽  
Penicillium Species ◽  
Allium Cepa L ◽  
Rot Disease ◽  
Very High

Abstract The purpose of the study was to explore the control of the onion black rot pathogen, Aspergillus niger, with Penicillium species as biological control agents. Fourteen Penicillium species were isolated from the rhizosphere of different plants. In dual culture agar plate assays, these isolates showed very high antagonistic effects on the growth of A. niger mycelium. Penicillium roqueforti and P. viridicatum greatly inhibited the growth of A. niger by 66% and 60%, respectively, followed by P. bilaii (57%) and P. olsonii (53%). However, it was also observed that the Penicillium species completely overgrew the A. niger colony. The study revealed that some species of the genus Penicillium possessed a high antagonistic effect on the onion black rot pathogen.

Aspergillus niger PSSG8 Mediated Bioleaching of Monazite for the Recovery of Rare Earth and other Metal Constituents

2015 ◽  
Vol 1130 ◽  
pp. 238-242
Author(s):  
Kishor Kumar Keekan ◽  
Jayesh C. Jalondhara
Keyword(s):  
Rare Earth ◽  
Aspergillus Niger ◽  
Fungal Growth ◽  
Fungal Mycelium ◽  
Mineral Particles ◽  
Shake Flasks ◽  
The Media ◽  
Stationary Conditions ◽  
And Control ◽  
Different Levels

Aspergillus nigerPSSG8 is assisted bioleaching of monazite to recover the constituent metals were investigated. Bioleaching was carried out using Bromfield (BM1/10P & BM-P) and sucrose media (SM1/10P & SM-P) with different levels of Phosphate under rotary and stationary conditions. The growth of theA. nigerPSSG8 significantly reduced the media pH to acidic. However, the media pH changed later in rotary shake flasks and were stable in roux bottles. A similar trend was observed for ORP. ORP increased as leaching progressed, but in rotary shake flasks ORP started decreasing after 15 days in Bromfield and 30 days in sucrose media. Fungal growth was maximum in SM when comparing to BM. The reduction and the omission of phosphate in both media did not significantly influence the culture parameters including the yield of biomass. ICP-AES analysis of leach liquor showed highest recovery of cerium (Ce) (1419 µg/L in BM-P) and thorium (Th) (182 µg/L in SM1/10P) in rotary conditions when comparing to the stationary conditions (229 µg/L Ce in BM 1/10P & 159 µg/L in SM-P). Reduction in the metal concentration was observed in the rotary shake flasks after 15 days of incubation. It was due to the biosorption of released metals by the fungal mycelium. Uranium was not detected in any of the media tested. SEM studies of the partially bioleached and control mineral particles show no changes in the surface features.

scholarly journals Mycoparasitic nature of Egyptian Trichoderma isolates and their impact on suppression Fusarium wilt of tomato

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
A. M. Nofal ◽  
Mohamed Abd El-Rahman ◽  
T. M. Abdelghany ◽  
Mahmoud Abd El-Mongy
Keyword(s):  
Biological Control ◽  
Plant Disease ◽  
Defense Mechanism ◽  
Disease Incidence ◽  
Culture Method ◽  
Dry Weight ◽  
Protective Role ◽  
Significant Inhibition ◽  
Dual Culture ◽  
Tomato Plants

Abstract Background Plant disease administration is difficult due to the soil-borne nature of the phytopathogens. Biological control of plant disease is a safe mode to avoid the problems related to fungal diseases that affect crops productivity. Results Twenty-three Trichoderma isolates were isolated from soil, surrounding healthy tomato roots from different regions in the Egyptian Governorate of Menoufia. Using a dual culture method to test the efficiency of Trichoderma isolates, the most effective isolate identified as Trichoderma atrovirde with percentage inhibition against Fusarium oxysporum f. sp. lycopersici (92.11%) and scanning electron microscope examination documented the mycoparasitic nature of T. atrovirde to F. oxysporum. Treatments with 10% filtrate T. atrovirde improved the growth aspects of tomato plants than the control plants or infected only, as well as the increase in phenol content (15.09 ug. g-1dry weight) and decreased disease incidence percentage (8%) than the plants infected only (60%). Conclusions This study clearly demonstrated that T. atrovirde had a significant inhibition against F. oxysporum. Greenhouse assays displayed the protective role of T. atrovirde inoculation directly against pathogen or indirectly related to the defense mechanism in the plant. So, this study recommends using T. atrovirde for biological control of wilt disease in tomato plants.

scholarly journals Aspergillus flavus Growth Inhibition and Aflatoxin B1 Decontamination by Streptomyces Isolates and Their Metabolites

Toxins ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 340
Author(s):  
Ixchel Campos-Avelar ◽  
Alexandre Colas de la Noue ◽  
Noël Durand ◽  
Guillaume Cazals ◽  
Véronique Martinez ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Aflatoxin B1 ◽  
Fungal Growth ◽  
Dual Culture ◽  
Bacterial Cells ◽  
Specific Production ◽  
Penicillium Verrucosum ◽  
Streptomyces Spp ◽  
Residual Toxicity ◽  
Promising Source

Aflatoxin B1 is a potent carcinogen produced by Aspergillus flavus, mainly during grain storage. As pre-harvest methods are insufficient to avoid mycotoxin presence during storage, diverse curative techniques are being investigated for the inhibition of fungal growth and aflatoxin detoxification. Streptomyces spp. represent an alternative as they are a promising source of detoxifying enzymes. Fifty-nine Streptomyces isolates and a Streptomyces griseoviridis strain from the commercial product Mycostop®, evaluated against Penicillium verrucosum and ochratoxin A during previous work, were screened for their ability to inhibit Aspergillus flavus growth and decrease the aflatoxin amount. The activities of bacterial cells and cell-free extracts (CFEs) from liquid cultures were also evaluated. Fifty-eight isolates were able to inhibit fungal growth during dual culture assays, with a maximal reduction going down to 13% of the control. Aflatoxin-specific production was decreased by all isolates to at least 54% of the control. CFEs were less effective in decreasing fungal growth (down to 40% and 55% for unheated and heated CFEs, respectively) and aflatoxin-specific production, with a few CFEs causing an overproduction of mycotoxins. Nearly all Streptomyces isolates were able to degrade AFB1 when growing in solid and liquid media. A total degradation of AFB1 was achieved by Mycostop® on solid medium, as well as an almost complete degradation by IX20 in liquid medium (6% of the control). CFE maximal degradation went down to 37% of the control for isolate IX09. The search for degradation by-products indicated the presence of a few unknown molecules. The evaluation of residual toxicity of the tested isolates by the SOS chromotest indicated a detoxification of at least 68% of AFB1’s genotoxicity.

scholarly journals MEKANISME ANTAGONIS LIMA ISOLAT Bacillus subtilis TERHADAP Colletotrichum capsici DAN C. gloeospoiroides IN VITRO

Agrin ◽  
2018 ◽  
Vol 21 (2) ◽  
Author(s):  
Nur Kholida Wulansari ◽  
Nur Prihatiningsih ◽  
Heru Adi Djatmiko
Keyword(s):  
Bacillus Subtilis ◽  
Culture Method ◽  
Dry Weight ◽  
Chili Pepper ◽  
Dual Culture ◽  
In Vitro Test ◽  
Colletotrichum Capsici ◽  
Randomized Design ◽  
Vitro Test

Colletotrichum capsici dan C. gloeospoiroides adalah jamur patogen penting pada cabai merah yang dapatmenurunkan produktivitas. Upaya untuk mengatasi permasalahan ini yaitu dengan pengendalian hayatimenggunakan Bacillus subtilis. Penelitian ini dilakukan untuk mengetahui daya hambat lima isolat B.subtilis yaituB46, B209, B211, B298, dan B315 terhadap patogen C. capsici dan C. gloeospoiroides asal tanaman cabai.Percobaan laboratorium dilakukan berdasarkan Rancangan Acak Lengkap dengan 5 ulangan. Pengujian in vitrodilakukan menggunakan teknik dual culture pada medium PDA. Hasil penelitian menunjukkan efektifitaspenghambatan C.capsici paling baik pada isolat B209 sebesar 34,25%. Efektifitas penghambatan C.gloeospoiroides pada isolat B211 sebesar 28,89%. Efektifitas penghambatan berpengaruh pada bobot keringmisellium dan morfologi hifa C. capsici dan C. gloeospoiroides. Morfologi hifa C. capsici dan C. gloeospoiroidesmengalami lisis, menebal, dan membengkak.Kata kunci: C. capsici, C. gloeospoiroides, B. subtilis, antagonistik, daya hambatABSTRACTColletotrichum capsici and C. gloeospoiroides are an important pathogen on red chili pepper and causedproductivity losses. Biological control using B. subtilis is an attempt to solve the problem. The objectives of thisstudy is to determines the inhibition ability of five B. subtilis isolates, i.e. B46, B209, B211, B298, dan B315against C. capsici and C. gloeospoiroides pathogens from chili pepper. Laboratory experinments arranged incomplete randomized design with five replication. Dual culture method used PDA medium on in vitro test. Resultshowed the best inhibition effectiveness of C.capsici on B209 isolates at 34.25%. The best inhibition effectivenessof C. gloeospoiroides on B211 isolates at 28,89%. The inhibition effectiveness affects mycellium dry weight andhypha morphology of C. capsici and C. gloeospoiroides. Hypha morphology of C. capsici and C. gloeospoiroidesis lysis, thickening, and swelling.Key words: C. capsici, C. gloeospoiroides, B. subtilis, antagonistic, inhibitor ability

scholarly journals Biological control in the germination of seeds from two species native of the Cerrado region

2020 ◽  
Author(s):  
J. C. Oliveira ◽  
J. F. Sales ◽  
A. Rubio-Neto ◽  
C. F. Silva ◽  
M. A. Soares ◽  
...  
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Aspergillus Niger ◽  
Tree Species ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Fungal Contamination ◽  
Antimicrobial Substances ◽  
Hard Seeds ◽  
Brevibacillus Brevis

Abstract Microorganisms have been efficiently used for the biological control of phytopathogens through the production of antimicrobial substances. However, the objectives of this work were: to study the germination of Butia purpurascens Glassman and Butia archeri Glassman seeds in different substrates, to select and identify the endophytic and rhizospheric bacterial isolates of B. purpurascens and B. archeri, and to perform antibiosis tests based on the isolated microorganisms of these tree species. No difference was found between the cultivation substrates for the percentages of germination, hard seeds, and fungal contamination in the B. purpurascens seeds. The Bacillus subtilis isolated showed the best capacity for suppressing the growth of the two deteriorative fungi tested in B. purpurascens seeds. No difference was found for inhibition of the growth of Aspergillus niger fungus (deteriorative fungus of B. archeri seeds) between the microorganisms with Bacillus sp. and Brevibacillus brevis compared to the control. In the microbiolization of B. purpurascens and B. archeri seeds performed with microbiological solutions produced from the endophytic and rhizospheric strains of Bacillus sp., no differences were observed in the percentages of germination and contamination by fungi. For B. archeri seeds, there was contamination by fungi and bacteria after one day of cultivation, primarily in the regions with lesions caused by the extraction and scarification process.

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